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The Qinghai-Tibetan Plateau has a booming tourism industry and an increasingly sophisticated road system. There is a paucity of studies quantifying the contributions of anthropogenic and natural factors to microplastic pollution in remote plateau areas. In this study, water and sediment samples were collected from eight lake tourist attractions and four remote lakes in northern and southern regions of the Qinghai-Tibetan Plateau. Microplastics were detected in all samples, with a mean abundance of 0.78 items/L in water and 44.98 items/kg in sediment. The abundance of microplastics in the study area was lower than previously observed in more populated areas of China. Small-sized (<1 mm and 1-2 mm), fiber, and transparent microplastics were predominant, with polyethylene and polypropylene microplastics as the primary polymer types. The compositions of microplastic communities indicated that tourism and road networks were the major sources of microplastics in the lakes. Distance-decay models revealed greater influence of environmental distances on microplastic community similarity than geographic distance. Compared to climate factors, urban spatial impact intensity and traffic flow impact played a leading role in the structuring of microplastic communities in lake water and sediment. Our findings provide novel quantitative insights into the role of various factors in shaping the distribution patterns of microplastic communities in plateau lakes.

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Water diversion has been widely utilized to enhance lake water quality and mitigate cyanobacterial blooms. However, previous studies have mainly focused on investigating the effects of water diversion on water quality or aquatic ecological health. Consequently, there is limited research investigating the combined impact of water diversion on the water quality and the ecological health of eutrophic lakes, and whether the WQI and phytoplankton assemblages demonstrate similar patterns following water diversion. In this study, the effects of water diversion on the ecosystem health of eutrophic lakes were comprehensively evaluated based on the WQI indices and phytoplankton assemblages during the NWDP-21 and WDP-22. The results showed that the annual mean of WQI increased from 52.02 to 54.36 after water diversion, which improved the water quality of the lake, especially NH3-N and TN decreased by 58.6% and 15.2%, respectively. The phytoplankton assemblages changed significantly before and after water diversion, and we observed that the total biomass of phytoplankton decreased by 12.3% and phytoplankton diversity indices (Shannon-Wiener diversity, Pielou evenness, and Simpson index) increased by 8.6%-8.9% after water diversion, with an improvement in the connectivity and stability of the phytoplankton. Notably, enhanced adaptations of rare sub-communities for resource use in water diversion environments, and water diversion inhibited the dispersal ability of dominant functional groups, and the effects of hydrological disturbances on the structure of phytoplankton assemblage favored the ecological health of eutrophic lakes. VPA analysis further reveals that water diversion alters the drivers of phytoplankton functional group biomass and phytoplankton diversity. The results of the PLS-PM analysis clarify that water diversion indirectly impacts the total phytoplankton biomass and phytoplankton diversity primarily by modifying light availability. Significant correlations are observed between the dominant functional groups biomass and diversity indices of WQI. The trends in changes observed in water quality indices and phytoplankton following water diversion align with the evaluation of water ecological health. This study provides valuable guidance for the ecological management of the diversion project in Yilong Lake and serves as a reference for similar projects in other lakes.

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As an emerging environmental pollutant, microplastics (MPs) have received widespread attention. Recently, studies examining microplastic pollution in plateau lakes have been increasing, but few have examined the distributions, sources, and fates of MPs in different plateau areas. In this work, the abundances and characteristics of MPs in surface waters and sediments in lakes of the Qinghai-Tibet Plateau (QTP) and Yunnan-Guizhou Plateau (YGP) were systematically investigated. The abundances of MPs in the lakes of the QTP ranges within 0.05-1.8 n/L in surface waters and 10-2643.7 n/kg in sediments. In the lakes of the YGP, the abundances of MPs ranged within 1.3-10.1 n/L in surface waters and 171.7-4260 n/kg in sediments. The dominant shape, color, and size class of MPs were fiber, transparent, and 0-0.5 mm in plateau lakes, respectively. MPs were mainly composed of polypropylene, polyethylene, and polyethylene terephthalate polymers. The different sources of MPs in the QTP and YGP lakes were mainly due to differences in human activities. The primary sources of microplastic pollution in the lakes of the QTP were tourism and atmospheric transport, while sewage discharge, agriculture, and fishing activities were the main sources of MPs in urban lakes of the YGP. Although the level of microplastic pollution in plateau lakes was relatively low, the sources should be identified and monitored so that the effects and extent of microplastic pollution in these fragile environments can be fully understood. This study provides a valuable dataset and theoretical basis for subsequent research on microplastic pollution in plateau lakes.

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Due to the large spatiotemporal variability in the processes controlling carbon emissions from lakes, estimates of global lake carbon emission remain uncertain. Identifying the most reliable predictors of CO2 and CH4 concentrations across different hydrological features can enhance the accuracy of carbon emission estimates locally and globally. Here, we used data from 71 lakes in Southwest China varying in surface area (0.01‒702.4 km2), mean depth (< 1‒89.6 m), and climate to analyze differences in CO2 and CH4 concentrations and their driving mechanisms between the dry and rainy seasons and between different mixing regimes. The results showed that the average concentrations of CO2 and CH4 in the rainy season were 23.9 ± 18.8 µmol L-1 and 2.5 ± 4.9 µmol L-1, respectively, which were significantly higher than in the dry season (10.5 ± 10.3 µmol L-1 and 1.8 ± 4.2 µmol L-1, respectively). The average concentrations of CO2 and CH4 at the vertically mixed sites were 24.1 ± 21.8 µmol L-1 and 2.6 ± 5.4 µmol L-1, being higher than those at the stratified sites (14.8 ± 13.4 µmol L-1 and 1.7 ± 3.5 µmol L-1, respectively). Moreover, the environmental factors were divided into four categories, i.e., system productivity (represented by the contents of total nitrogen, total phosphorus, chlorophyll a and dissolved organic matter), physicochemical factors (water temperature, Secchi disk depth, dissolved oxygen and pH value), lake morphology (lake area, water depth and drainage ratio), and geoclimatic factors (altitude, wind speed, precipitation and land-use intensity). In addition to the regression and variance partitioning analyses between the concentrations of CO2 and CH4 and environmental factors, the cascading effects of environmental factors on CO2 and CH4 concentrations were further elucidated under four distinct hydrological scenarios, indicating the different driving mechanisms between the scenarios. Lake morphology and geoclimatic factors were the main direct drivers of the carbon concentrations during the rainy season, while they indirectly affected the carbon concentrations via influencing physicochemical factors and further system productivity during the dry season; although lake morphology and geoclimatic factors directly contributed to the carbon concentrations at the vertically mixed and stratified sites, the direct effect of system productivity was only observed at the stratified sites. Our results emphasize that, when estimating carbon emissions from lakes at broad spatial scales, it is essential to consider the influence of precipitation-related seasons and lake mixing regimes.

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The death of aquatic and terrestrial organisms caused by cyanobacterial blooms has been a topic of considerable concern since the 19th century. Microcystin-LR (MC-LR) produced by cyanobacterial blooms threaten natural ecosystems and human health. Therefore, establishing an effective monitoring and early warning system to detect MC-LR in water bodies is crucial. However, rapidly and intuitively assessing the distribution traits of MC-LR in lakes is a challenging task due to the complexities and expenses associated with conventional detection methods. To overcome these technical limitations, we introduce a novel and effective method for evaluating the distribution of MC-LR in lakes. This method is achieved by using a fluorescence probe (BAD) technology, marking the first application of this technology in evaluating the distribution of MC-LR in natural lake environments. The probe BAD is endowed with unique functions through clever functionalization modification. Experimental results exhibit that BAD has different fluorescence signals at various lake sampling points. The correlation analysis of fluorescence data and physicochemical indicators determines that the fluorescence data of the probe exhibit good correlation with MC-LR, implying that BAD is capable of detecting MC-LR in lakes. Moreover, the introduction of fluorescence technology to achieve the intuitive distribution of MC-LR in the entire plateau lake. This study provides a new method for evaluating the distribution of MC-LR in plateau lakes. It opens a new avenue for exploring the relationship between cyanobacterial blooms and MC-LR in natural waters.

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Microplastic pollution in freshwater environments has received increasing attention. However, limited research on the occurrence and distribution of microplastics in plateau lakes. This study investigated the microplastic characteristics and influencing factors in lakes with different land cover types on the Inner Mongolia Plateau. Results showed that microplastic abundance ranged from 0.5 to 12.6 items/L in water and 50-325 items/kg in sediments. Microplastics in water were predominantly polypropylene (50.5%), fragments (40.5%), and 50-200 µm (66.7%). High-density (27.9%), fibrous (69.3%), and large-sized microplastics (47.7%) were retained primarily in lake sediments. The highest microplastic abundance in water was found in cropland lakes and grassland lakes, while that in sediments was in descending order of desert lakes > cropland lakes > grassland lakes > forest-grassland lakes. Differences among lake types suggest that agriculture, tourism, and atmospheric transport may be critical microplastic sources. Microplastic distribution was positively correlated with farmland and artificial surface coverage, showing that land cover types related to human activities could exacerbate microplastic pollution in lakes. Redundancy analysis showed that ammonia nitrogen and pH were the key physicochemical factors affecting microplastic distribution in lakes, indicating the potential sources of microplastics in lakes and the uniqueness of microplastic occurrence characteristics in desert saline-alkaline lakes, respectively.

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The presence of organic matter in lakes profoundly impacts drinking water supplies, yet treatment processes involving coagulants and disinfectants can yield carcinogenic disinfection by-products. Traditional assessments of organic matter, such as chemical oxygen demand (CODMn) and biochemical oxygen demand (BOD5), are often time-consuming. Alternatively, optical measurements of dissolved organic matter (DOM) offer a rapid and reliable means of obtaining organic matter composition data. Here we employed DOM optical measurements in conjunction with parallel factor analysis to scrutinize CODMn and BOD5 variability. Validation was performed using an independent dataset encompassing six lakes on the Yungui Plateau from 2014 to 2016 (n = 256). Leveraging multiple linear regressions (MLRs) applied to DOM absorbance at 254 nm (a254) and fluorescence components C1-C5, we successfully traced CODMn and BOD5 variations across the entire plateau (68 lakes, n = 271, R2 > 0.8, P < 0.0001). Notably, DOM optical indices yielded superior estimates (higher R2) of CODMn and BOD5 during the rainy season compared to the dry season and demonstrated increased accuracy (R2 > 0.9) in mesotrophic lakes compared to oligotrophic and eutrophic lakes. This study underscores the utility of MLR-based DOM indices for inferring CODMn and BOD5 variability in plateau lakes and highlights the potential of integrating in situ and remote sensing platforms for water pollution early warning.

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Understanding the genomic differentiation between marine and non-marine aquatic microbes remains a compelling question in ecology. While previous research has identified several lacustrine lineages within the predominantly marine Roseobacteraceae family, limited genomic data have constrained our understanding of their ecological adaptation mechanisms. In this study, we isolated four novel Yoonia strains from a brackish lake on the Tibetan Plateau. These strains have diverged from their marine counterparts within the same genus, indicating a recent habitat transition event from marine to non-marine environments. Metabolic comparisons and ancestral genomic reconstructions in a phylogenetic framework reveal metabolic shifts in salinity adaptation, compound transport, aromatics degradation, DNA repair, and restriction systems. These findings not only corroborate the metabolic changes commonly observed in other non-marine Roseobacters but also unveil unique adaptations, likely reflecting the localized metabolic changes in responses to Tibetan Plateau environments. Collectively, our study expands the known genomic diversity of non-marine Roseobacteraceae lineages and enhances our understanding of microbial adaptations to lacustrine ecosystems.

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Dissolved organic matter (DOM) is the largest carbon pool and directly affects the biogeochemistry in lakes. In the current study, fourier transform ion cyclotron mass spectrometry (FT-ICR-MS) combined with fluorescent spectroscopy was used to assess the molecular composition and driving mechanism of DOM in 22 plateau lakes in Mongolia Plateau Lakes Region (MLR), Qinghai Plateau Lakes Region (QLR) and Tibet Plateau Lakes Region (TLR) of China. The limnic dissolved organic carbon (DOC) content ranged from 3.93 to 280.8 mg L-1 and the values in MLR and TLR were significantly higher than that in QLR. The content of lignin was the highest in each lake and showed a gradually decreasing trend from MLR to TLR. Random forest model and structural equation model implied that altitude played an important role in lignin degradation while the contents of total nitrogen (TN) and chlorophyll a (Chl-a) have a great influence on the increase of DOM Shannon index. Our results also suggested that the inspissation of DOC and the promoted endogenous DOM production caused by the inspissation of nutrient resulted in a positive relationship between limnic DOC content and limnic factors such as salinity, alkalinity and nutrient concentration. From MLR to QLR and TLR, the molecular weight and the number of double bonds gradually decreased but the humification index (HIX) also decreased. In addition, from the MLR to the TLR, the proportion of lignin gradually decreased, while the proportion of lipid gradually increased. Both above results suggested that photodegradation was dominated in lakes of TLR, while microbial degradation was dominated in lakes of MLR.

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Algal extracellular organic matter (EOM), a major fraction of the dissolved organic matter found in eutrophic plateau lakes, can act as a photosensitizer to drive the abiotic oxidation of Mn(II). This process has the potential to generate reactive Mn(III) and influence the fate of organic pollutants. In this study, the photodegradation of 17α-ethinylestradiol (EE2) in the presence of Mn(II) and EOM was investigated with emphasis on the photogeneration mechanism of Mn(III). The results indicated that Mn(II) can accelerate EE2 photodegradation in EOM solution owing to the photogeneration of reactive Mn(III), and the enhancement was greater at higher Mn(II) concentrations. The generation of reactive Mn(III) was mainly attributable to the action of superoxide radical generated by photosensitization of EOM. In addition, the photodegradation of EE2 was slower at higher pH, possibly because of the deactivation of Mn(III) under alkaline conditions. Single-electron transfer was an indispensable process in the photodegradation. The differences in fluorophore content, pH, and NO3- concentrations are all important determinants for EE2 photodegradation in natural waters. The information obtained in this research would contribute to the understanding of reactions between Mn(II) and EOM, and provide new insights into the behaviors of reactive Mn(III) in eutrophic water irradiated by sunlight.

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Lugu Lake is one of the best plateau lakes in China in terms of water quality, but in recent years the eutrophication of Lugu Lake has accelerated due to high nitrogen and phosphorus loads. This study aimed to determine the eutrophication state of Lugu Lake. Specifically, the spatio-temporal variations of nitrogen and phosphorus pollution during the wet and dry seasons were investigated in Lianghai and Caohai, and the primary environmental effect factors were defined. Adopting the endogenous static release experiments and the exogenous improved export coefficient model, a novel approach (a combination of internal and external sources) was developed for the estimation of nitrogen and phosphorus pollution loads in Lugu Lake. It was indicated that the order of nitrogen and phosphorus pollution in Lugu Lake was Caohai > Lianghai and dry season > wet season. Dissolved oxygen (DO) and chemical oxygen demand (CODMn) were the main environmental factors causing nitrogen and phosphorus pollution. Endogenous nitrogen and phosphorus release rates in Lugu Lake were 668.7 and 42.0 t/a, respectively, and exogenous nitrogen and phosphorus input rates were 372.7 and 30.8 t/a, respectively. The contributions of pollution sources, in descending order, were sediment > land-use categories > residents and livestock breeding > plant decay, of which sediment nitrogen and phosphorus loads accounted for 64.3 % and 57.4 %, respectively. Regulating the endogenous release of sediment and obstructing the exogenous input from shrubland and woodland are emphasized for the management of nitrogen and phosphorus contamination in Lugu Lake. Thus, this study can serve as a theoretical foundation and technical guide for eutrophication control in plateau lakes.

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Microbes serve as important components of ecosystem services and biogeochemical processes in plateau lakes. However, the features of microbiota assembly, abundance and diversity in plateau lakes remain unclear. The microbial communities in surface water from nine plateau lakes in Yunnan Plateau, southwestern China, in the dry and rainy seasons were explored using 16S rRNA gene and 18S rRNA gene sequencing. The results showed that the bacterial community compositions were homogeneous while those of micro-eukaryotes were heterogeneous in plateau lakes. In both seasons, the bacterial phyla of Proteobacteria and Actinobacteriota predominated in oligotrophic lakes. The mesotrophic lakes were dominated by Proteobacteria, Actinobacteriota, Bacteroidota and Cyanobacteria. The eutrophic lakes were mainly dominated by Proteobacteria, Actinobacteriota and Cyanobacteria. The phylum SAR_k_norank had the major micro-eukaryotes in these plateau lakes. The alpha-diversity of bacteria declined in the rainy season, while that of micro-eukaryotes varied from lake to lake. The drivers of microbiotic community assembly in the dry season were identified as nutrient level-related factors. In the rainy season, however, the microbiota in oligotrophic lakes were related to nutrient levels. Microbial communities were driven by precipitation in mesotrophic and eutrophic lakes with large water volumes, while those in lakes with small water volumes were regulated by nutrient level-related factors. Our findings pose first and unique insights into the microbiota of the nine plateau lakes in Yunnan Plateau, providing important ecological knowledge for these lakes with different characteristics.

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The extensive application of phosphorus fertilizers to croplands and the aggregation of towns and villages around plateau lakes has resulted in the continuous accumulation of phosphorus in the soil profile and the discharge of phosphorus pollutants, which causes phosphorus pollution in shallow groundwater around the lakes. The phosphorus entering the lake with shallow underground runoff in the region around the lake also affects the water quality safety of plateau lakes. The spatiotemporal differences in phosphorus concentrations in 452 shallow groundwater samples and the driving factors were analyzed by monitoring wells in croplands and residential areas around the eight lakes in Yunnan province during the rainy and dry seasons from 2019 to 2021. The results showed that seasonal changes and land use influenced phosphorus concentrations and their composition in shallow groundwater. The concentration of phosphorus in shallow groundwater in the rainy season was higher than that in the dry season, and it was also greater in cropland than that in residential areas. DTP was the dominant form of TP, accounting for 75%-81%, and DIP was the dominant form of DTP, accounting for 74%-80%. Nearly 30% of the samples around the eight lakes had TP concentrations exceeding the surface water Class Ⅲ standard (GB 3838); the exceeded rates of phosphorus in groundwater around the Erhai Lake (52%), Qiluhu Lake (45%), Xingyun Lake (42%), and Dianchi Lake (29%) were far higher than those of Yangzonghai Lake (16%), Fuxianhu Lake (13%), Chenghai Lake (6%), and Yilonghu Lake (5%). The key driving factors of phosphorus concentrations in shallow groundwater were water-soluble phosphorus (WEP), water content (MWC), soil organic matter (SOM), total nitrogen (TN), pH in the soil profile, and pH and groundwater level in the shallow groundwater (P<0.05). The increases in WEP, SOM, TN, and MWC in the soil and pH in groundwater significantly increased the concentrations of DIP and DTP in shallow groundwater, whereas the decrease in groundwater level significantly reduced the concentrations of DTP and DIP in the groundwater.

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The long-term accumulation, burial and release of nutrients, such as carbon (C), nitrogen (N), and phosphorus (P) in lacustrine sediments are responsible for the global lake eutrophication. Interpretation of the spatiotemporal sedimentary record of nutrients (C, N, and P) in contrasting trophic level of lakes is helpful for understanding the evolutionary process of water eutrophication. Based on the radiochronology of 210Pbex and 137Cs, a comparative study of spatial and temporal concentrations, burial of total organic carbon (TOC), total nitrogen (TN), and total phosphorus (TP), the sources of organic matter were conducted using sediment cores from two plateau lakes Dianchi (DC) and Fuxian (FX) of SW China. Results showed that concentrations and burial of C, N, and P in sediments of DC, a shallow hypertrophic lake with the maximum depth of 5.8 m, were both higher than those in FX, an oligotrophic deep lake with the maximum depth of 155.0 m. For both lakes the molar ratio of TOC/TN increased in the sediments moving from north to south. The values of TOC/TN molar ratios increased over time in DC and were higher than in FX. The extremely high values of TOC/TN appeared in the central and southern parts of FX, indicating the impacts of accumulation effect and sediment focusing in the deeper region and indirect supplement from the Lake Xingyun (XY), an adjoining lake connected with FX via the Gehe River. Time-integrated sources identification in DC indicated the contribution of allochthonous sources was dominant over the past few decades, which contributed to the increased trophic level of the lake. The comparison of relationships of carbon accumulation rates (CAR), nitrogen accumulation rates (NAR), and phosphorous accumulation rates (PAR), the ratios of N/P and the utilizations of N and P fertilizer between DC and FX implied that both of N and P inputs should be limited for reducing the trophic level, but N control was predominant in comparison with P for both lakes. The results indicated that caution is required in plateau lakes to limit transition from oligotrophic to eutrophic in these lakes.

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Climate change globally accelerates the shrinkage of inland lakes, resulting in increases in both water salinity and dissolved inorganic carbon (DIC). The increases of salinity and DIC generate contrasting effects on microbial primary producers and primary production, however, their combined effects remain unclear in aquatic ecosystems. We hypothesized that increased DIC mitigates the constraints of enhanced salinity on microbial primary producers and primary production. To test this, we employed isotope labeling and molecular methods to explore primary production and four dominant types of microbial primary producers (form IA, IB, IC and ID) in lakes on the Tibetan Plateau. Results showed that DIC was positively correlated with the abundance of the form IAB and ID microbial primary producers and primary production (all P < 0.001) and offset salinity constraints. Structural equation models elucidated that DIC substantially enhanced primary production by stimulating the abundance of form ID microbial primary producers. The abundance of form ID primary producers explained more variations (14.6%) of primary production than form IAB (6%) and physicochemical factors (6.8%). Diatoms (form ID) played a determinant role in primary production in the lakes by adapting to high DIC and high salinity. Our findings suggest that inland lakes may support higher primary productivity in future climate change scenarios.

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Qinghai-Tibet Plateau (QTP) Lake Region has largest abundance and size distribution of lakes in China. Being relatively away from major human activities, the water quality of these lakes has not attracted concerns in the past. However, dramatic climate change and intensified anthropogenic activities over the past 30 years have exerted multiple pressures on the water environment of the lakes, resulting in elevated nutrient concentrations in major freshwater lakes of the region. Rapid water quality deterioration and eutrophication of the lakes were first found in Lake Hurleg in the northeast of the plateau. Analyses of driving forces associated with these changes indicate that both the intrinsic characteristics of the QTP lakes and climate change were responsible for the vulnerability to human activities than other lakes in different regions of China, with accelerated urbanization and extensive economic development in the lake basin playing a decisive role in creating water pollution events. Under combination pressures from both natural and anthropogenic effect, the increasing rate of nutrient concentrations in Lake Hurleg has been 53-346 times faster than in Lake Taihu and Lake Dianchi during the deterioration stage. The result suggests the current development mode of Lake Hurleg basin is not suitable for setting protection targets for the QTP lake region more broadly due to its extremely poor environmental carrying capacity. To stop worsening the lake water environment condition, it is necessary to review the achievements made and lessons learned from China's fight against lake pollution and take immediate measures, inform policies into the development mode in the QTP lake region, and avoid irreversible consequences and ensure good water quality in the "Asian Water Tower."

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Salinity is one of the most important environmental factors influencing bacterial plankton communities in lake waters, while its influence on bacterial interactions has been less explored. Here, we investigated the influence of salinity on the bacterial diversity, interactions and community structure in Tibetan Plateau lakes. Our results revealed that saline lakes (salinity between 0.5 and 50 g/L) harboured similar or even higher bacterial diversity compared with freshwater lakes (< 0.5 g/L), while hyper-saline lakes (> 50 g/L) exhibited the lowest diversity. Network analysis demonstrated that hyper-saline lakes exhibited the highest network complexity, with higher total correlation numbers (particularly the negative correlations), but lower network module numbers than freshwater and saline lakes. Furthermore, salinity dominantly explained the bacterial community structure variations in saline lakes, while those in freshwater and hyper-saline lakes were predominately explained by water temperature and geospatial distance, respectively. The core operational taxonomic units (OTUs), which were ubiquitously present in all lakes, were less sensitive to enhancing salinity than the indicative OTUs whose presence was dependent on lake type. Our findings offer a new understanding of how salinity influences bacterial community in plateau lakes.

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In aquatic systems, dissolved organic matter (DOM) plays an important role in regulating the reactivity and transport of environmental pollutants. Fluorescence excitation-emission matrix (EEM) analysis combined with fluorescence regional integration (FRI) and parallel factorial analysis (PARAFAC) were applied to investigate the composition, humification degree and depth-dependent variation of DOM in Lake Baihua, China. The results showed that humic-like materials with more than 60% of percentage fluorescence response (Pi,n) were dominant in DOM. The molecular complexity and structural condensation of PARAFAC components showed the order of C1 (humic-like components) > C3 (protein-like components) > C4 (fulvic-like components) > C2 (fulvic-like components). The lower maximum fluorescence intensities (Fmax) of components in surface layers were attributed to photo-oxidation of DOM by radiation. With depths less than 16 m, the Fmax increases and decreases were attributed to accumulation of recalcitrant humic substances and microbial/abiotic degradation of particulate DOM, respectively. A combination of biological and humification indices could be used as indicators for distinguishing different degrees of humification and sources of DOM. DOM from Lake Baihua mainly originated from an aquatic bacterial source that consisted of an important/intermediate autochthonous component with a weak humic character. The fluorescence indices of PI,n/PII,n, PI+II+IV,n/PIII+V,n, C2, C1/C2 and C2/(C3+C4) were more suitable to determine the humification degree. Stronger humic characters and higher humification degree for DOM were present at depths of 10-13 m. The fluorescence spectroscopy combined with fluorescence indices is convenient to investigate depth-dependent DOM characteristics and to assess water quality or pollution risk in lake systems.

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This study established the hydrochemistry and stable isotope variations in three lakes, namely brackish Zigetang Co, freshwater Cona and Ranwu lakes located in the central and southeastern Tibetan Plateau of China. Vertical profile fluctuations in the water column, such as temperature and dissolved oxygen (DO) concentration, displayed significant differences which were linked to the thermocline. The hydrochemistry of the three lakes showed that [Formula: see text] as the dominant anion, whereas Na+ is the main cation in Zigetang Co and Cona lake and Ca2+ is the prevailing cation in Ranwu lake. In Zigetang Co, K+ and Na+ concentrations decreased by 42 % from 1999 to 2012, caused by the enlargement of the lake area, documented by field investigations carried out in 1998, 2002, 2006 and 2012. The δ13CDIC and δ18OH2O values analysed from the three lakes varied from -6.0 to 2.0 ‰, and from -14.8 to -6.4 ‰, respectively. The closed Zigetang lake showed higher δ13CDIC and δ18OH2O values compared to those of the rivers, the semi-closed Cona and open Ranwu lakes. The δ13CDIC values of lake water in Zigetang Co were mainly controlled by CO2 exchange between lake water and atmosphere; the δ18OH2O values were dominated by the evaporation/freshwater input ratios.

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Elevated environmental pressures negatively affect the bacterial community structure. However, little knowledge about the nonlinear responses of spatially related environmental variable across multiple plateau lake ecosystems on bacterioplankton communities has been gathered. Here, we used 454 pyrosequencing of 16S rRNA genes to study the associations of bacterial communities in terms of environmental characteristics as well as the potentially ecological threshold-inducing shifts of the bacterial community structure along the key environmental variables based on hypothesized structural equation models and the SEGMENTED method in 21 plateau lakes. Our results showed that water transparency was the major driving force and that total nitrogen was more significant than total phosphorus in determining the taxon composition of the bacterioplankton community. Significant community threshold estimates for bacterioplankton were observed at 7.36 for pH and 25.6% for the percentage of the agricultural area, while the remarkable change point of the cyanobacteria community structure responding to pH was at 7.74. Furthermore, the findings indicated that increasing nutrient loads can induce a distinct shift in dominance from Proteobacteria to Cyanobacteria, as well as a sharp decrease and adjacent increase when crossing the change point for Actinobacteria and Bacteroidetes along the gradient of the agricultural area.

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