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Rhizosphere engineering approach is considered a quantum leap in plant sciences. The current study focused on investigating rhizobacterial efficiency to mobilize bioavailable phosphate from insoluble-phosphate source. Four efficient phosphate-solubilizing bacterial isolates, i.e., Pseudomonas songnenensis (GR3), Stutzerimonas stutzeri (HH2), Bacillus bingmayongensis (KH3), and Achromobacter aegrifaciens (MH1) were selected for the current study. Interactions between various physiological parameters and phosphate solubilization efficiency of isolates revealed that glucose significantly facilitated phosphorus solubilization at 37 ℃, with media having pH 7 and 0.5% phosphorous. Additionally, positive correlation among P-solubilization potential, acids produced, and pH was observed. Plant microbe-interaction analysis was performed to evaluate the efficiency of these bacterial isolates on various morpho-physiological responses of Zea mays L. For this purpose, various concentrations of tricalcium phosphate (TCP) (0, 10, 20, 30, 40, and 50 mM) were applied to plants in the presence and absence of bacterial isolates. The results showed that lower phosphate levels (10 and 20 mM) trigger shoot development and improve plant weight and leaf formation whereas higher phosphate concentrations (30 mM and above) stimulated the development of longer root system. The bacterial isolates, KH3 and HH2, were observed as efficient phosphate-solubilizing bacteria (PSB) that positively stimulated various plant growth and biochemical attributes over untreated plants. At lower phosphate levels, substantial increase of 92, 65, and 200% in shoot length, fresh weight, and number of leaves was recorded with bacterial isolate HH2, whereas, at 30 mM TCP, increase of 165% was observed in root length of plants treated with bacterial isolate KH3 compared to control. Similarly, at lower phosphate levels, increment of 57.3, 76.7, and 217% in phosphate, protein, and auxin content was recorded in plants treated with bacterial isolate HH2, and increase of 188.8% in total soluble carbohydrates was observed in plants treated with bacterial isolate KH3 as compared to control. Contrarily, increment in total chlorophyll content was most substantial (207%) by the bacterial isolate KH3 when provided with 30 mM TCP. Hence, the current study reviled that the use of these phosphates (KH3 and HH2)-solubilizing PGPR, as an efficient phytostimulator used for crop production in the replacement of chemical fertilizers, is carcinogenic and deteriorating our eco-system.

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For agricultural safety and sustainability, instead of synthetic fertilizers the eco-friendly and inexpensive biological applications include members of plant-growth-promoting rhizobacteria (PGPR) genera, Pseudomonas spp. will be an excellent alternative option to bioinoculants as they do not threaten the soil biota. The effect of phosphate solubilizing bacteria (PSB) Pseudomonas aeruginosa (MK 764942.1) on groundnuts' growth and yield parameters was studied under field conditions. The strain was combined with a single super phosphate and tested in different combinations for yield improvement. Integration of bacterial strain with P fertilizer gave significantly higher pod yield ranging from 7.36 to 13.18% compared to plots where sole inorganic fertilizers were applied. Similarly, the combined application of PSB and inorganic P fertilizer significantly influenced plant height and number of branches compared to sole. However, a higher influence of phosphorous application (both PSB and P fertilizer) observed both nodule dry weight and number of nodules. Combined with single super phosphate (100% P) topped in providing better yield attributing characters (pod yield, haulm yield, biomass yield, 1000 kernel weight, and shelling percentage) in groundnut. Higher oil content was also recorded with plants treated with Pseudomonas aeruginosa combined with single super phosphate (SSP) (100% P). Nutrients like nitrogen (N), phosphorous (P), and potassium (K) concentrations were positively influenced in shoot and kernel by combined application. In contrast, Ca, Mg, and S were found to be least influenced by variations of Phosphorous. Plants treated with Pseudomonas aeruginosa and lower doses of SSP (75% P) recorded higher shoot and kernel P. We found that co-inoculation with PSB and SSP could be an auspicious substitute for utilizing P fertilizer in enhancing yield and protecting nutrient concentrations in groundnut cultivation. Therefore, PSB can be a good substitute for bio-fertilizers to promote agricultural sustainability.

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Fluorescent ligands have proved to be powerful tools in the study of G protein-coupled receptors in living cells. Here we have characterized a new fluorescent ligand PSB603-BY630 that has high selectivity for the human adenosine A2B receptor (A2BR). The A2BR appears to play an important role in regulating immune responses in the tumor microenvironment. Here we have used PSB603-BY630 to monitor specific binding to A2BRs in M1- and M2-like macrophages derived from CD14+ human monocytes. PSB603-BY630 bound with high affinity (18.3 nM) to nanoluciferase-tagged A2BRs stably expressed in HEK293G cells. The ligand exhibited very high selectivity for the A2BR with negligible specific-binding detected at NLuc-A2AR, NLuc-A1R, or NLuc-A3R receptors at concentrations up to 500 nM. Competition binding studies showed the expected pharmacology at A2BR with the A2BR-selective ligands PSB603 and MRS-1706 demonstrating potent inhibition of the specific binding of 50 nM PSB603-BY630 to A2BR. Functional studies in HEK293G cells using Glosensor to monitor Gs-coupled cyclic AMP responses indicated that PSB603-BY630 acted as a negative allosteric regular of the agonist responses to BAY 60-6583. Furthermore, flow cytometry analysis confirmed that PSB603-BY630 could be used to selectively label endogenous A2BRs expressed on human macrophages. This ligand should be an important addition to the library of fluorescent ligands which are selective for the different adenosine receptor subtypes, and will enable study of the role of A2BRs on immune cells in the tumor microenvironment.

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Background: Several local regional anesthesia regimes have been described in the literature to reduce post-surgical pain following total knee arthroplasty (TKA), but it is unclear which regime has the best analgetic effect combined with the best motor function. The aim of this study was to determine if patients with infiltration between the popliteal artery and capsule of the posterior knee (IPACK) combined with an adductor canal block (SACB) had less pain, better motor function, and less opioid consumption after TKA than patients with a femoral nerve block (FNB) combined with a popliteal sciatic nerve block (PSB). Methods: In a retrospective cohort analysis, 342 patients following primary TKA were examined; 175 patients were treated with an IPACK combined with a SACB, and 167 patients with a femoral FNB combined with a PSB. The outcome parameters postoperative pain (visual analogue scale (VAS) for mobilization and at rest, functional recovery, opioid consumption, hospital discharge, and complications were analyzed and compared between both groups. Results: The IPACK/SACB group had a higher postoperative need for opioids despite higher doses of ropivacaine compared to the FNB/PSB group, accompanied by higher VAS scores. Patients' satisfaction was equal between the groups. Both groups showed comparable mobilization rates and walking distances following TKA. Conclusions: IPACK/SACB showed equal results compared to FNB/PSB for mobilization rates and patients' satisfaction following TKA without a reduction in opioid consumption.

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Rubber trees are a commercial cash crop, and the milky latex or polyisoprene they produce is the natural source of rubber. Little is known about the bacterial populations found in active zone of latex-bearing caulosphere. We employed a tailored cloud microbial bioinformatic approach for the identification and potential hypothetical ecological roles of an uncultured endophytic hidden bacterial community in the active zone of the latex-bearing caulosphere of Hevea brasiliensis. Small pieces of slivers were collected from healthy plant from the village: Belonia, South Tripura, rubber plantation in Northeastern India. These uncultured bacteria were identified using the V3-V4 hypervariable amplicon region of the 16 S rDNA gene. A total of 209,586 contigs have been generated. EasyMAP Version 1.0, a cloud-based microbial bioinformatics tool with an integrated QIIME2 pipeline, was used to analyze contigs. We detected 15 phyla and 91 OTUs (operational taxonomic units). Proteobacteria (73.5%) was the most enriched phylum, followed by Firmicutes (13.8%), Bacteroidetes (5.2%), and Actinobacteria (3.2%). Ammonia oxidizers, sulfate reducers, dehalogenation, chitin degradation, nitrite reducers, and aromatic hydrocarbon degraders were the most prevalent functional categories in the active zones of caulosphere. Furthermore, Gammaproteobacteria (49.2%) and Erwinia (29.19%) were the most abundant classes and genera of endophytic bacterial communities. Thus, the presence of a substantial amount of phosphate-solubilizing Gammaproteobacteria (PSB) may stimulate growth, increase plant resilience, suppress disease, and aid in the rubber and sugar breakdown. This is the first report of microbial endophytes associated with Hevea caulosphere.

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The development of a highly sensitive, synthetically simple and economical SERS substrate is technically very important. A fast, economical, sensitive and reproducible CuNPs@AgNPs@ Porous silicon Bragg reflector (PSB) SERS substrate was prepared by electrochemical etching and in situ reduction method. The developed CuNPs@AgNPs@PSB has a large specific surface area and abundant "hot spot" region, which makes the SERS performance excellent. Meanwhile, the successful synthesis of CuNPs@AgNPs can not only modulate the plasmon resonance properties of nanoparticles, but also effectively prolong the time stability of Cu nanoparticles. The basic performance of the substrate was evaluated using rhodamine 6G (R6G). (Detection limit reached 10-15 M, R2 = 0.9882, RSD = 5.3 %) The detection limit of Forchlorfenuron was 10 µg/L. The standard curve with a regression coefficient of 0.979 was established in the low concentration range of 10 µg/L -100 µg/L. This indicates that the prepared substrates can accomplish the detection of pesticide residues in the low concentration range. The prepared high-performance and high-sensitivity SERS substrate have a very promising application in detection technology.

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BACKGROUND: Child sexual abuse (CSA) and problematic sexual behavior (PSB) are worldwide phenomena that occur across all ages. Kindergarten teachers' proactive involvement can be crucial to the prevention, disclosure and intervention of CSA and PSB. However, research on their experiences of contending with CSA and PSB remains limited. OBJECTIVE: This study examines kindergarten teachers' experiences in Israel with the CSA and PSB of their students. PARTICIPANTS AND SETTING: Semi-structured interviews were conducted with 31 teachers: 11 secular Jewish, seven religious Jewish, nine Druze Arab, and four Muslim Arab. METHODS: A qualitative analysis was conducted using the interview transcripts as data. RESULTS: The analysis revealed three themes illustrating teachers' professional transformations regarding their knowledge of these phenomena: 1) initial shock, uncertainty and sense of responsibility when exposed to CSA and PSB due to missing knowledge, 2) implementation of prevention and intervention strategies regarding CSA and PSB, and 3) embracing a social role to disseminate CSA and PSB knowledge. The findings indicated that the majority of the teachers went from overwhelming shock and fear due to a lack of knowledge in coping with CSA and PSB to a sense of responsibility as a community leader. CONCLUSIONS: The fragmentation of the Israeli education system isolates kindergartens, and the lack of training and education for the teachers left them alone when contending with the CSA and PSB of their students. Nevertheless, the participants exhibited remarkable agency and resourcefulness, gaining the necessary knowledge and acting as knowledge agents within their communities.

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The growing global population has led to a heightened need for food production, and this rise in agricultural activity is closely tied to the application of phosphorus-based fertilizers, which contributes to the depletion of rock phosphate (RP) reserves. Considering the limited P reserves, different approaches were conducted previously for P removal from waste streams, while the adsorption of ions is a novel strategy with more applicability. In this study, a comprehensive method was employed to recover phosphorus from wastewater by utilizing biochar engineered with minerals such as calcium, magnesium, and iron. Elemental analysis of the wastewater following a batch experiment indicated the efficiency of the engineered biochar as an adsorbent. Subsequently, the phosphorus-enriched biochar, hereinafter (PL-BCsb), obtained from the wastewater, underwent further analysis through FTIR, XRD, and nutritional assessments. The results revealed that the PL-BCsb contained four times higher (1.82%) P contents which further reused as a fertilizer supplementation for Brassica napus L growth. PL-BCsb showed citric acid (34.03%), Olsen solution (10.99%), and water soluble (1.74%) P desorption. Additionally, phosphorous solubilizing bacteria (PSB) were incorporated with PL-BCsb along two P fertilizer levels P45 (45 kg ha-1) and P90 (90 kg ha-1) for evaluation of phosphorus reuse efficiency. Integrated application of PL-BCsb with half of the suggested amount of P45 (45 kg ha-1) and PSB increased growth, production, physiological, biochemical, and nutritional qualities of canola by almost two folds when compared to control. Similarly, it also improved soil microbial biomass carbon up to four times, alkaline and acid phosphatases activities both by one and half times respectively as compared to control P (0). Furthermore, this investigation demonstrated that waste-to-fertilizer technology enhanced the phosphorus fertilizer use efficiency by 55-60% while reducing phosphorus losses into water streams by 90%. These results have significant implications for reducing eutrophication, making it a promising approach for mitigating environmental pollution and addressing climate change.

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Chickpeas (Cicer arietinum L.) are a valuable legume crop due to their nutritional value. To maintain chickpea productivity and avoid the adverse effects of climate change on soil and plant processes, it is crucial to address demand. Achieving this necessitates implementing sustainable agricultural practices incorporating the use of biostimulants, adaptable crops for arid conditions, as well as pest and disease-resistant crops that are sustainable over time. Three varieties of chickpeas were analysed to determine the effect of two different biostimulant application methods on both germination and vegetative growth. Possible effects due to location were also examined by conducting tests at two different sites. Significant variations in biostimulant response were evident only during the germination period, but not during the vegetative development stage, where the observed statistical differences were influenced more by the location or variety of chickpeas employed. Furthermore, this study examined the effect of biostimulants on nutrient cycling within the soil-plant microbiota system. Nitrogen-fixing bacteria (NFB) are present in the soil of chickpea crops at an order of magnitude of 107 CFU/g DS. Additionally, an average concentration of 106 CFU/g DS of phosphorus-mobilising bacteria was observed. Applying biostimulants (BioE) to seeds resulted in a successful germination percentage (GP) for both Amelia (AM) and IMIDRA 10 (IM) varieties.

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Extraction and utilization of effective phosphorus from solid waste have been an important approach for alleviating phosphorus resource shortage. The extraction of available phosphorus by microbial method with low cost, mild conditions and simple process has been drawing attention from the majority of research scholars. However, relevant studies on special microbial communities for effective phosphorus extraction from solid waste are less. In this work，a functional Bacillus_cereus strain screened from phosphate tailings, phosphate ore and forest rhizosphere soil was inoculated into phosphate tailings (PT), modified phosphate tailings (IS) and highland red soil (SS). Compared with SS, the water-holding properties, fertility, leaching toxicity and microbial community diversity of PT and IS with and without bacteria were analyzed. PT+, SS+ and IS+ (after adding bacteria to PT, SS and IS) showed moderately alkaline pH, and the available phosphorus content enhanced by 31.73%, 20.05% and 39.41% respectively. The leaching toxicity phosphate of PT+ and IS + decreased by 4.89 mg/kg and 2.61 mg/kg respectively, while that of SS + increased by 5.45 mg/kg, indicating differences in the phosphorus solubilization mechanism of Bacillus_cereus for different soils. Furthermore, the modification and bacteria treatment improved the relative abundance of Pedobacter, Alcaligenaceae and Pseudomonas, thus enhancing the phosphorus solubility of the PT bacterial community. This work may achieve efficient utilization and ecological restoration of phosphorus tailings-based soil and contribute to long-term sustainable agricultural development.

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Phosphate solubilizing bacteria (PSB) has been considered an environmental-friendly phosphate fertilizer without cadmium (Cd) input into soils, but its possibility of Cd fixation in soil needs to be explored. Since direct inoculation results in a rapid decline of the population and activity, we immobilized Bacillus megaterium with maize straw biochar (B-PSB) and investigated its feasibility in remediating Cd-contaminated soil. Pot experiments showed that the application of B-PSB significantly ameliorated the growth of Brassica chinensis under Cd stress, with a fresh weight increased by 59.08% compared to the Cd-control. B-PSB reduced Cd accumulation in Brassica chinensis by 61.69%, and promoted the uptake of P and N by 134.97% and 98.71% respectively. Microbial community analysis showed B-PSB recruited more plant growth-promoting bacteria in near-rhizosphere soil, which provides a favorable microenvironment for both PSB and crops. Column leaching experiments verified that B-PSB achieved the dissolution of stable P while fixing Cd. Batch tests further revealed that biochar served as a successful carrier facilitating the growth of B. megaterium and Cd immobilization. Given the widespread Cd contamination in agricultural soils, our results indicate that B-PSB is a promising soil amendment to secure food safety.

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BACKGROUND: The release of organic acids (OAs) is considered the main mechanism used by phosphate-solubilizing bacteria (PSB) to dissolve inorganic phosphate in soil. Nevertheless, little is known about the effect of individual OAs produced by a particular PSB in a soil-plant system. For these reasons, the present work aimed at investigating the effect of Enterobacter sp. strain 15S and the exogenous application of its OAs on (i) the solubilization of tricalcium phosphate (TCP), (ii) plant growth and (iii) P nutrition of cucumber. To this purpose two independent experiments have been performed. RESULTS: In the first experiment, carried out in vitro, the phosphate solubilizing activity of Enterobacter 15S was associated with the release of citric, fumaric, ketoglutaric, malic, and oxalic acids. In the second experiment, cucumber plants were grown in a Leonard jar system consisting of a nutrient solution supplemented with the OAs previously identified in Enterobacter 15S (jar's base) and a substrate supplemented with the insoluble TCP where cucumber plants were grown (jar's top). The use of Enterobacter 15S and its secreted OAs proved to be efficient in the in situ TCP solubilization. In particular, the enhancement of the morpho-physiological traits of P-starved cucumber plants was evident when treated with Enterobacter 15S, oxalate, or citrate. The highest accumulation of P in roots and shoots induced by such treatments further corroborated this hypothesis. CONCLUSION: In our study, the results presented suggest that organic acids released by Enterobacter 15S as well as the bacterium itself can enhance the P-acquisition by cucumber plants.

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Phosphorus (P) is often the limiting factor for plant growth because of its low mobility and availability in soils. Phosphate-solubilizing bacteria (PSB) have been shown to increase the availability of soil P fractions, thereby promoting plant growth. We herein investigated the effects of PSB on P availability in two important Chinese soil types: Lateritic red earths (La) and Cinnamon soils (Ci). We initially isolated 5 PSB strains and assessed their effects on soil P fractions. PSB mainly increased moderately labile P in La and labile P in Ci. We then selected the most promising PSB isolate (99% similarity with Enterobacter chuandaensis) and examined its effects on P accumulation in maize seedlings. The results obtained showed that plant P accumulation increased in response to a PSB inoculation in both soil types and the combination of the PSB inoculation and tricalcium phosphate fertilization in La significantly enhanced P accumulation in plant shoots. The present study demonstrated that the PSB isolates tested differed in their ability to mobilize P from distinct P fertilizers and that PSB isolates have potential as a valuable means of sustainably enhancing seedling growth in Chinese agricultural soils.

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Bioremediation is one of the most promising strategies for heavy metal immobilization. A new remediation system was demonstrated in this research, which combined phosphate solubilizing bacteria (PSB) with nZVI@Carbon/Phosphate (nZVI@C/P) composite to remediate lead contaminated soil. Experimental results indicated that the new system (nZVI@C/P + PSB) could effectively convert the labile Pb into the stable fraction after 30 days of incubation, which increased the maximum residual fraction percentage of Pb by 70.58%. The characterization results showed that lead may exist in the forms of Pb5(PO4)3Cl, PbSO4 and 3PbCO3·2Pb(OH)2·H2O in the soil treated with nZVI@C/P + PSB. Meanwhile, soil enzyme activities and Leclercia abundance were enhanced in the treated soil compared with CK during the incubation time. In addition, the specialized functions (e.g. ABC transporters, siderophore metabolism, sulfur metabolism and phosphorus metabolism) in PSB and nZVI@C/P + PSB group were also enhanced. These phenomena proved that the key soil metabolic functions may be maintained and enhanced through the synergistic effect of incubated PSB and nZVI@C/P. The study demonstrated that this new bioremediation system provided feasible way to improve the efficacy for lead contaminated soil remediation.

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Phosphorus (P), an essential macronutrient for all life on Earth, has been shown to be a vital limiting nutrient element for plant growth and yield. P deficiency is a common phenomenon in terrestrial ecosystems across the world. Chemical phosphate fertilizer has traditionally been employed to solve the problem of P deficiency in agricultural production, but its application has been limited by the non-renewability of raw materials and the adverse influence on the ecological health of the environment. Therefore, it is imperative to develop efficient, economical, environmentally friendly and highly stable alternative strategies to meet the plant P demand. Phosphate-solubilizing bacteria (PSB) are able to improve plant productivity by increasing P nutrition. Pathways to fully and effectively use PSB to mobilize unavailable forms of soil P for plants has become a hot research topic in the fields of plant nutrition and ecology. Here, the biogeochemical P cycling in soil systems are summarized, how to make full use of soil legacy P via PSB to alleviate the global P resource shortage are reviewed. We highlight the advances in multi-omics technologies that are helpful for exploring the dynamics of nutrient turnover and the genetic potential of PSB-centered microbial communities. Furthermore, the multiple roles of PSB inoculants in sustainable agricultural practices are analyzed. Finally, we project that new ideas and techniques will be continuously infused into fundamental and applied research to achieve a more integrated understanding of the interactive mechanisms of PSB and rhizosphere microbiota/plant to maximize the efficacy of PSB as P activators.

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It is well-known that phosphate-solubilizing bacteria (PSB) promote crop growth and yield. The information regarding characterization of PSB isolated from agroforestry systems and their impact on wheat crops under field conditions is rarely known. In the present study, we aim to develop psychrotroph-based P biofertilizers, and for that, four PSB strains (Pseudomonas sp. L3, Pseudomonas sp. P2, Streptomyces sp. T3, and Streptococcus sp. T4) previously isolated from three different agroforestry zones and already screened for wheat growth under pot trial conditions were evaluated on wheat crop under field conditions. Two field experiments were employed; set 1 includes PSB + recommended dose of fertilizers (RDF) and set 2 includes PSB - RDF. In both field experiments, the response of the PSB-treated wheat crop was significantly higher compared to the uninoculated control. In field set 1, an increase of 22% in grain yield (GY), 16% in biological yield (BY), and 10% in grain per spike (GPS) was observed in consortia (CNS, L3 + P2) treatment, followed by L3 and P2 treatments. Inoculation of PSB mitigates soil P deficiency as it positively influences soil alkaline phosphatase (AP) and soil acid phosphatase (AcP) activity which positively correlated with grain NPK %. The highest grain NPK % was reported in CNS-treated wheat with RDF (N-0.26%, P-0.18%, and K-1.66%) and without RDF (N-0.27, P-0.26, and K-1.46%), respectively. All parameters, including soil enzyme activities, plant agronomic data, and yield data were analyzed by principal component analysis (PCA), resulting in the selection of two PSB strains. The conditions for optimal P solubilization, in L3 (temperature-18.46, pH-5.2, and glucose concentration-0.8%) and P2 (temperature-17°C, pH-5.0, and glucose concentration-0.89%), were obtained through response surface methodology (RSM) modeling. The P solubilizing potential of selected strains at <20°C makes them a suitable candidate for the development of psychrotroph-based P biofertilizers. Low-temperature P solubilization of the PSB strains from agroforestry systems makes them potential biofertilizers for winter crops.

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INTRODUCTION: In an era of workplace safety where psychosocial risks are widely recognized as occupational hazards, emerging research has sought to clarify the impact of these risks and the requisite interventions for improving psychosocial safety climate and reducing psychological injury risk. METHOD: The construct of psychosocial safety behavior (PSB) provides a novel framework for emerging research that seeks to apply a behavior-based safety approach to workplace psychosocial risks across several high-risk industries. This scoping review aims to provide a synthesis of existing literature on PSB, including its development as a construct and application in workplace safety interventions to date. RESULTS: Although a limited number of studies of PSB were identified, the findings of this review provide evidence for growing cross-sector applications of behaviorally-focused approaches to improving workplace psychosocial safety. In addition, the identification of a broad spectrum of terminology surrounding the construct of PSB provides evidence of key theoretical and empirical gaps, with implications for future intervention-based research to address emerging areas of focus.

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The current investigation depicts the individual and synergistic effects of two important plant growth promoting microbial groups viz. Plant Growth Promoting Rhizobacteria (PGPR) and Phosphate Solubilizing Bacteria (PSB) in alleviating the phytotoxic impacts of chromium in Vigna radiata (L) R. Wilczek (green gram) seedlings. Cr6+ (100 ppm) treatment caused a stiff decline of about 44%, 72%, 68%, and 49% reduction in root and shoot length as well as leaf number and leaf area respectively as compared to control after 90 d of exposure. However, combined amendment with PGPR and PSB causes a significant amelioration of Cr toxicity though doubling the shoot length and leaf area with a 4 times increase in root length and leaf number after 90 d of growth. Total chlorophyll synthesis showed a 68% reduction in Cr6+ (100 ppm) which was ameliorated by combined treatments of PGPR and PSB. It showed a 123% increased total chlorophyll content than Cr6+ (100 ppm) whereas individual application of PGPR and PSB showed a 46% and 27% increase respectively. Combined application of PGPR and PSB with a toxic dose of Cr showed significant boosting alleviation ability and indicates its ameliorative role for abatement of Cr-induced toxicity.

The present research work suggests the combined potential of PGPR and PSB in agriculture and can be adopted as a sustainable strategy for the alleviation of Cr toxicity stress in mining soil.

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Potassium (K) and phosphorus (P) are the important macronutrients needed for the plant development, but it is widely present in an insoluble form for the plant's uptake. In order to increase the productivity, biofertilisers play crucial role in plant growth enhancement. Our present work focused to isolate potassium-phosphate solubilizing bacteria from the agricultural soil of tomato cultivated soil. Potassium and phosphate solubilization and degradation of monocrotophos was estimated spectrophotometrically. Out of thirteen isolates, two isolates proved to be the best P and K solubilizers. The bacterial isolates (SDKVG02 and SDKVG04) were optimized to obtain maximum P and K solubilization of 57.5 mg L-1 and 15.07 mg L-1 by the isolates. Pot experiments were conducted using SDKVG 02 and 04, immobilized on carrier materials, peat proving the best carrier with the total average green gram and chick pea length of 11.66 ± 0.0666 22.22 ± 0.0577. The MCP degradation percentage was achieved at 80 ppm of MCP with 75.8% and 64.10% by SDKVG 02 and SDKVG 04. Furthermore, production of organic acids such as malic acid, phthalic acid, ascorbic acid, nicotinic acid, and tartaric acid paves solubilization of P and K. The isolates were recognized based on 16S rRNA gene sequencing as Enterobacter hormaechei- SDKVG-02, Enterobacter cloacae SDKVG- 04. The KSB-PSB isolates also express N-fixing activity which is proved through In-silico analysis. It is worth to highlight SDKVG 02 and 04 would be potent biofertiliser exploited in increasing the soil fertility and crop productivity as well in degradation of monocrotophos present in the soil.

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BACKGROUND: Rhinoplasty with autologous costal cartilage (ACC) is followed by severe pain in the chest. Ultrasound-guided (USG) serratus anterior plane block (SAPB), in combination with parasternal block (PSB), was earlier reported to be highly efficacious in relieving pain associated with thoracic anterior lateral surgery. However, it is unclear whether it is effective for pain relief after ACC harvest. METHODS: Sixty-four patients, aged 18 to 60, who received rhinoplasty with ACC, were randomly separated into a SAPB+PSB or SAPB group. The analyzed parameters of both groups included the rest and coughing numerical rating scale (NRS) pain scores of the chest and the NRS pain scores of the nose at postoperative 2, 4, 8, 12, 24, and 48 hours, oral rescue analgesic usage, side effect incidence and patient satisfaction, etc. RESULTS: Thirty patients per group were recruited for analysis. The rest and coughing NRS scores of the chest and the NRS scores of the nose at postoperative 2, 4, 8, 12 h were lower in the SAPB+PSB group, compared to the SAPB group (all P < 0.05). However, these scores were comparable between the two groups at postoperative 24 and 48 h (all P > 0.05). Additionally, relative to the SAPB group, the oral rescue analgesic usage was drastically lower (P < 0.05), the postoperative nausea and vomiting (PONV) incidence was diminished (P < 0.05), and the patient satisfaction was markedly higher (P < 0.001) in the SAPB+PSB group. CONCLUSION: USG-SAPB, in combination with improved PSB, is superior to SAPB alone in relieving pain after ACC harvest in rhinoplasty. LEVEL OF EVIDENCE II: This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .

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