RESUMEN

This study demonstrates the utility of a PCR-based DNA sequencing approach to make a specific diagnosis of onchocerciasis in a returned traveller. Although a clinical diagnosis was not possible, the surgical excision of a suprascapular nodule from this patient, combined with an histological examination of this nodule and PCR-based sequencing of DNA from a nematode from this lesion solved the case. The analysis of DNA sequence data confirmed the presence of Onchocerca volvulus infection, supporting an effective treatment-clinical management strategy for the patient.

Asunto(s)

Onchocerca/genética , Oncocercosis/diagnóstico , Oncocercosis/parasitología , Reacción en Cadena de la Polimerasa , Análisis de Secuencia de ADN , Adulto , Animales , Biopsia , Manejo de la Enfermedad , Femenino , Genes Mitocondriales , Humanos , Onchocerca/clasificación , Oncocercosis/terapia , Filogenia , Reacción en Cadena de la Polimerasa/métodos