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OBJECTIVE: The purposes of this study were to examine to (1) the relation between OA and sarcopenia and to identify the most practical, easily accessible, and inexpensive method for investigating sarcopenia; (2) evaluation of sarcopenia risk factors in patients with OA. DESIGN: One hundred two patients with clinical and radiological diagnosis of knee osteoarthritis and 33 healthy control subjects were included in the study and all subjects were evaluated for sarcopenia. Dual-X-ray absorptiometry (DEXA) is used to measure body composition parameters and muscle thickness measurements with ultrasonography for diagnosis of sarcopenia. RESULTS: The mean age of the group with sarcopenia was statistically higher than the other two groups (p < 0.001). The weight, body mass index (BMI), waist circumference, upper-mid-arm circumference, thigh, and leg circumference of osteoarthritis (OA) patients with sarcopenia were statistically lower than those of non-sarcopenic and control group (p < 0.01, p < 0.001). Body composition parameter results showed that sarcopenic patients had statistically lower values as fat mass, lean body mass, and skeletal muscle index (p < 0.001, p = 0.001, p < 0.001, respectively) than those of non-sarcopenic and control group and fat mass index values (p = 0.012) are lower than the non-sarcopenic group. With respect to sarcopenia, the effect of adiponectin and leptin levels were not detected. It was determined that body composition values measured with DEXA, ultrasonographic measures, isokinetic muscle strength assessment, handgrip strength, and gait speed had predictive values for sarcopenia. CONCLUSIONS: We found that patients with sarcopenic OA were older, weaker, undernourished, and restricted in their level of physical activity in the study. Among the methods of determining sarcopenia, ultrasound becomes prominent with its practical, cheap, and easily accessible features. We think that our results will increase the awareness of the presence of sarcopenia in OA patients.
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Osteoartritis de la Rodilla , Sarcopenia , Humanos , Sarcopenia/complicaciones , Sarcopenia/diagnóstico por imagen , Fuerza de la Mano , Osteoartritis de la Rodilla/complicaciones , Osteoartritis de la Rodilla/diagnóstico por imagen , Obesidad/complicaciones , Absorciometría de Fotón , Músculo EsqueléticoRESUMEN
AIMS: To analyze the potential role of synovial fluid peptidase activity as a measure of disease burden and predictive biomarker of progression in knee osteoarthritis (KOA). METHODS: A cross-sectional study of 39 patients (women 71.8%, men 28.2%; mean age of 72.03 years (SD 1.15) with advanced KOA (Ahlbäck grade ≥ 3 and clinical indications for arthrocentesis) recruited through the (Orthopaedic Department at the Complejo Asistencial Universitario de León, Spain (CAULE)), measuring synovial fluid levels of puromycin-sensitive aminopeptidase (PSA), neutral aminopeptidase (NAP), aminopeptidase B (APB), prolyl endopeptidase (PEP), aspartate aminopeptidase (ASP), glutamyl aminopeptidase (GLU) and pyroglutamyl aminopeptidase (PGAP). RESULTS: Synovial fluid peptidase activity varied significantly as a function of clinical signs, with differences in levels of PEP (p = 0.020), ASP (p < 0.001), and PGAP (p = 0. 003) associated with knee locking, PEP (p = 0.006), ASP (p = 0.001), GLU (p = 0.037), and PGAP (p = 0.000) with knee failure, and PEP (p = 0.006), ASP (p = 0.001), GLU (p = 0.037), and PGAP (p < 0.001) with knee effusion. Further, patients with the greatest functional impairment had significantly higher levels of APB (p = 0.005), PEP (p = 0.005), ASP (p = 0.006), GLU (p = 0.020), and PGAP (p < 0.001) activity, though not of NAP or PSA, indicating local alterations in the renin-angiotensin system. A binary logistic regression model showed that PSA was protective (p = 0.005; Exp (B) 0.949), whereas PEP (p = 0.005) and GLU were risk factors (p = 0.012). CONCLUSION: These results suggest synovial fluid peptidase activity could play a role as a measure of disease burden and predictive biomarker of progression in KOA. Cite this article: Bone Joint Res 2020;9(11):789-797.
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Osteoarthritis (OA) is the most prevalent disabling disease, affecting quality of life and contributing to morbidity, particularly during aging. Current treatments for OA are limited to palliation: pain management and surgery for end-stage disease. Innovative approaches and animal models are needed to develop curative treatments for OA. Here, we investigated the naked mole-rat (NMR) as a potential model of OA resistance. NMR is a small rodent with the maximum lifespan of over 30 years, resistant to a wide range of age-related diseases. NMR tissues accumulate large quantities of unique, very high molecular weight, hyaluronan (HA). HA is a major component of cartilage and synovial fluid. Importantly, both HA molecular weight and cartilage stiffness decline with age and progression of OA. As increased polymer length is known to result in stiffer material, we hypothesized that NMR high molecular weight HA contributes to stiffer cartilage. Our analysis of biomechanical properties of NMR cartilage revealed that it is significantly stiffer than mouse cartilage. Furthermore, NMR chondrocytes were highly resistant to traumatic damage. In vivo experiments using an injury-induced model of OA revealed that NMRs were highly resistant to OA. While similarly treated mice developed severe cartilage degeneration, NMRs did not show any signs of OA. Our study shows that NMRs are remarkably resistant to OA, and this resistance is likely conferred by high molecular weight HA. This work suggests that NMR is a useful model to study OA resistance and NMR high molecular weight HA may hold therapeutic potential for OA treatment.
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Osteoartritis/fisiopatología , Animales , Modelos Animales de Enfermedad , Ratas TopoRESUMEN
PURPOSE: The purpose of this study was to compare the femoral mechanical-anatomical (FMA) and mechanical femoro-tibial (MFT) angles in an osteoarthritic population using the 2D (two dimension) and the 3D (three dimension) EOS low-dose biplanar radiographic system (EOS). METHODS: FMA and MFT angles were calculated in 127 adults with osteoarthritis. In 2D, FMA angle was measured between the femoral mechanical axis and the femoral anatomical axis, and MFT angle between the femoral mechanical axis and the tibial mechanical axis. In 3D, the measurement of FMA angle consisted of identifying specific anatomical landmarks on X-rays. MFT angle was then measured between the femoral mechanical axis and the tibial mechanical axis. The distribution of 2D and 3D values was assessed in terms of means and variances. RESULTS: Mean age was 69 ± 12 years. A total of 10% of the patients having a 3D FMA angle between 4° and 7° have a 2D-measured FMA over or underestimated. Particularly, FMA values tend to be underestimated in women in 2D. Finally, we found that men showed a tendency to a more varus morphology, with MFT values being significantly underestimated in 2D. CONCLUSIONS: The EOS 3D reconstruction system is a reliable method to measure FMA and MFT angles in an osteoarthritic population.