RESUMEN
In this study, a new non-enzymatic carbon paste biosensor was developed for the determination of Bisphenol-A (BPA) based on Multiwalled Carbon Nanotube (MWCNT) modified Myoglobin (Mb). The measurement principle of the biosensor was developed based on the inhibition effect of BPA on the heme group of myoglobin in the presence of hydrogen peroxide. With the designed biosensor, measurements were taken in the potential range of (-0.15 V & +0.65 V) using the differential pulse voltammetry (DPV) method in the medium containing K4[Fe(CN)6]. The linear range for BPA was determined to be 100-1000 µM. Response time was calculated as 16 s. The limit of detection was set at 89 µM. As a result, it has been proven that MWCNT modified myoglobin based biosensor is an alternative method that can be used for BPA determination, giving very sensitive and fast results.
Asunto(s)
Técnicas Biosensibles , Nanotubos de Carbono , Mioglobina , Técnicas Biosensibles/métodos , Compuestos de Bencidrilo , FenolesRESUMEN
A commercial arsenic field kit designed to measure inorganic arsenic (iAs) in water was modified into a field deployable method (FDM) to measure iAs in rice. While the method has been validated to give precise and accurate results in the laboratory, its on-site field performance has not been evaluated. This study was designed to test the method on-site in Malawi in order to evaluate its accuracy and precision in determination of iAs on-site by comparing with a validated reference method and giving original data on inorganic arsenic in Malawian rice and rice-based products. The method was validated by using the established laboratory-based HPLC-ICPMS. Statistical tests indicated there were no significant differences between on-site and laboratory iAs measurements determined using the FDM (pâ¯=â¯0.263, άâ¯=â¯0.05) and between on-site measurements and measurements determined using HPLC-ICP-MS (pâ¯=â¯0.299, άâ¯=â¯0.05). This method allows quick (within 1â¯h) and efficient screening of rice containing iAs concentrations on-site.