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Traditional nitrogen fertilizers (TNF), such as urea, percolate easily in arid fields, posing low nitrogen use efficiency (NUE) and a high non-point pollution risk. Controlled-release fertilizers (CRF) exhibit significantly lower deep seepage, rendering it a favorable choice in arid fields due to its ability to enhance NUE through slow-release mechanisms. However, current models do not fully account for the soil nitrogen dynamics and crop interactions under controlled-release conditions, and lack quantification. This study improved the APSIM model by adjustment the urea hydrolysis rate to assess the impact of CRF and TNF applications on soil health, crop growth, and water quality. Calibration and validation were conducted through experiments in the Hetao Irrigation District of China from 2019 to 2020, with different nitrogen application rates (135, 225, and 315 kg/ha). The model accurately simulated soil NO3-N concentration (SNC), cumulative NO3-N leaching (CNL), nitrogen uptake (NU), and sunflower yield. During the validation process, R2 and Nash-Sutcliffe efficiency (NSE) values were both above 0.75. Results indicated that the average SNC, NU, and yield under CRF application were significantly higher than those under TNF application, with increases of 38.62%, 44.92%, and 18.38%, respectively. Notably, the proportion of soil nitrogen available (PSNA), a novel metric proposed in this study, was 159.50% higher in the 0-40 cm soil layer with CRF compared to TNF. Additionally, CNL and NO3-N leaching loss rate (NLLR) decreased by 25.76% and 25.77%, respectively. Scenario simulations indicated that the optimal fertilization strategy for this region is to use 180-193.5 kg/ha of CRF with a release period of 80-85.5 d to balance agricultural productivity and ecological protection. This study confirms the significant advantages of CRF in enhancing yield, improving nitrogen management, and promoting environmental sustainability, providing a scientific basis for CRF management strategies and supporting the shift towards more efficient and environmentally friendly agricultural practices.

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This study successfully established Iron Sulfide-Mediated mixotrophic Partial Denitrification/Anammox system, achieving nitrogen and phosphorus removal efficiency of 97.26% and 78.12%, respectively, with COD/NO3--N of 1.00. Isotopic experiments and X-ray Photoelectron Spectroscopy analysis confirmed that iron sulfide enhanced autotrophic Partial Denitrification performance. Meanwhile, various sulfur valence states functioned as electron buffers, reinforcing nitrogen and sulfur cycles. Microbial community analysis indicated reduced heterotrophic denitrifiers (OLB8, OLB13) under lower COD/NO3--N, creating more niche space for autotrophic bacteria and other heterotrophic denitrifiers. The prediction of functional genes illustrated that iron Sulfide upregulated genes related to carbon metabolism, denitrification, anammox and sulfur oxidation-reduction, facilitating the establishment of carbon-nitrogen-sulfur cycle. Furthermore, this cycle primarily produced electrons via nicotinamide adenine dinucleotide and sulfur oxidation-reduction processes, subsequently utilized within the electron transfer chain. In summary, the Partial Denitrification/Anammox system under the influence of iron sulfide achieved effient nitrogen removal by expediting electron transfer through the carbon-nitrogen-sulfur cycle.

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The research on the deviations caused by different resolutions is relevant to the study of spatial scale effects. In 2018, spatial interpolations were performed using the removal ratios of the TN, NH4-N, and NO3-N of the layers of different resolutions, respectively. Based on the mean and the standard deviation, the area, shape, and position were obtained for four levels related to the removal ratios of the three nitrogen forms. The linear and 6th function fitting methods were used to reveal the differences in nitrogen removal in wetland water at different spatial resolutions. The results showed that a resolution of 25 times the original was the key scale of the spatial effects. Due to the fact that 52 of the 72 functions did not reach a significant level (P < 0.05), the spatial scale effect of the nitrogen removal was mainly characterized by disorderly fluctuations. The results have a certain extrapolation value for the analysis of spatial scale effects. PRACTITIONER POINTS: The resolution difference was not sufficient to change the spatial pattern of the geographic phenomena. The resolution of 25 times the original was the important scale for determining spatial effects. When studying the spatial scale effects caused by differences in resolution, it was necessary to comprehensively consider various resolutions.

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Nitrate (NO3-N), as one of the ubiquitous contaminants in groundwater worldwide, has posed a serious threat to public health and the ecological environment. Despite extensive research on its genesis, little is known about the differences in the genesis of NO3-N pollution across different concentrations. Herein, a study of NO3-N pollution concentration classification was conducted using the Shaying River Basin as a typical area, followed by examining the genesis differences across different pollution classifications. Results demonstrated that three classifications (0-9.98 mg/L, 10.14-27.44 mg/L, and 28.34-136.30 mg/L) were effectively identified for NO3-N pollution using Jenks natural breaks method. Random forest exhibited superior performance in describing NO3-N pollution and was thereby affirmed as the optimal explanatory method. With this method coupling SEMs, the genesis of different NO3-N pollution classifications was proven to be significantly different. Specifically, strongly reducing conditions represented by Mn2+, Eh, and NO2-N played a dominant role in causing residual NO3-N at low levels. Manure and sewage (represented by Cl-) leaching into groundwater through precipitation is mainly responsible for NO3-N in the 10-30 mg/L classification, with a cumulative contribution rate exceeding 80 %. NO3-N concentrations >30 mg/L are primarily caused by the anthropogenic loads stemming from manure, sewage, and agricultural fertilization (represented by Cl- and K+) infiltrating under precipitation in vulnerable hydrogeological conditions. Pathway analysis based on standardized effect and significance further confirmed the rationality and reliability of the above results. The findings will provide more accurate information for policymakers in groundwater resource management to implement effective strategies to mitigate NO3-N pollution.

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Nitrosation of pyridine alkaloids in tobacco generates tobacco-specific nitrosamines (TSNAs), which are notable toxicants in tobacco products and smoke. Burley tobacco, a chloroplast- and nitrogen (N)-deficient phenotype that accumulates high levels of nitrate-nitrogen (NO3-N) in its leaves, is particularly susceptible to TSNAs formation. In this study, reciprocal pot and field grafting experiments were conducted using burley tobacco Eyan No.1 and flue-cured tobacco K326 to investigate whether grafting burley tobacco scions on flue-cured tobacco rootstocks could enhance pigment biosynthesis and photosynthesis, while reducing the NO3-N level in burley tobacco leaves. Grafting burley tobacco scions on flue-cured tobacco rootstocks significantly increased the total pigment content, photosynthetic rate, biomass, nitrate reductase and glutamine synthetase activities, as well as ammonium-nitrogen (NH4-N), total soluble and reducing sugar, and soluble protein levels in burley tobacco leaves compared with burley tobacco self-rooting, while decreasing the NO3-N level and nitrate-N to total N ratio. Transcriptomic analysis revealed that grafting resulted in upregulated expression of genes involved in starch, sucrose, porphyrin, chlorophyll, and N metabolism, as well as carbon fixation and carotenoid biosynthesis. The findings suggest that grafting on high N use efficiency rootstock is an exceptionally promising means of decreasing NO3-N accumulation by improving photosynthesis and N metabolism in the scion, thereby reducing the levels of harmful TSNAs.

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Human activity has doubled the turnover rate of the terrestrial nitrogen cycle, leading to a series of environmental problems. A little-studied nitrogen source in terrestrial and aquatic environments is the nitrogen release associated with rock strata. Southwest China features the largest continuous karsts in the world, featuring a fragile ecological environment but abundant coal resources. The current study selected a typical coal mining area to evaluate the migration and transformation of nitrogen related to coal mining in surface watershed. The findings reveal that the total nitrogen in coal seams was as high as 10,162.3 mg/kg, mainly in the form of organic nitrogen, followed by NH4+-N, while the content of NO3--N was negligible. Based on the isotope fractionation and the co-evolution between Δ15NNO3-NH4 and δ15N-NO3-/δ15N-NH4+, coal mining changed the coal seams' oxidation-reduction state, resulting in the mineralization of organic nitrogen to NH4+-N. Next, NH4+-N gradually oxidized to NO3--N. Various forms of coal-origin nitrogen may be leached out by acid mine drainage (AMD), potentially contributing >10 % of NO3--N and 90 % of NH4+-N to the surface river. Another nitrogen source that requires serious consideration is the wide use of ammonium nitrate explosives in coal mining, as blasting residues may contribute about another 10 % to NO3--N in surface water. Since organic nitrogen accounts for >90 % of extractable nitrogen, the release of coal-origin nitrogen may contribute much more to the total nitrogen in surface water than to NO3--N. Based on the fractionation of nitrogen and oxygen isotopes of nitrate, low-pH AMD promotes the volatilization of nitrate in the form of nitric acid. The conversion of different forms of nitrogen in AMD will be the focus of future attention.

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Nitrogen (N) limits plant productivity, and its uptake and assimilation may be regulated by N sources, N assimilating enzymes, and N assimilation genes. Mastering the regulatory mechanisms of N uptake and assimilation is a key way to improve plant nitrogen use efficiency (NUE). However, it is poorly known how these factors interact to influence the growth process of pecans. In this study, the growth, nutrient uptake and N assimilation characteristics of pecan were analyzed by aeroponic cultivation at varying NH4 +/NO3 - ratios (0/0, 0/100,25/75, 50/50, 75/25,100/0 as CK, T1, T2, T3, T4, and T5). The results showed that T4 and T5 treatments optimally promoted the growth, nutrient uptake and N assimilating enzyme activities of pecan, which significantly increased aboveground biomass, average relative growth rate (RGR), root area, root activity, free amino acid (FAA) and total organic carbon (TOC) concentrations, nitrate reductase (NR), nitrite reductase (NiR), glutamine synthetase (GS), glutamate synthase (Fd-GOGAT and NADH-GOGAT), and glutamate dehydrogenase (GDH) activities. According to the qRT-PCR results, most of the N assimilation genes were expressed at higher levels in leaves and were mainly significantly up-regulated under T1 and T4 treatments. Correlation analysis showed that a correlation between N assimilating enzymes and N assimilating genes did not necessarily exist. The results of partial least squares path model (PLS-PM) analysis indicated that N assimilation genes could affect the growth of pecan by regulating N assimilation enzymes and nutrients. In summary, we suggested that the NH4 +/NO3 - ratio of 75:25 was more beneficial to improve the growth and NUE of pecan. Meanwhile, we believe that the determination of plant N assimilation capacity should be the result of a comprehensive analysis of N concentration, N assimilation enzymes and related genes.

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Ionic liquids (ILs) are thought to have negative effects on human health. Researchers have explored the effects of ILs on zebrafish development during the early stages, but the intergenerational toxicity of ILs on zebrafish development has rarely been reported. Herein, parental zebrafish were exposed to different concentrations (0, 12.5, 25, and 50 mg/L) of [Cn mim]NO3 (n = 2, 4, 6) for 1 week. Subsequently, the F1 offspring were cultured in clean water for 96 h. [Cn mim]NO3 (n = 2, 4, 6) exposure inhibited spermatogenesis and oogenesis in F0 adults, even causing obvious lacunae in the testis and atretic follicle oocytes in ovary. After parental exposure to [Cn mim]NO3 (n = 2, 4, 6), the body length and locomotor behavior were measured in F1 larvae at 96 hours post-fertilization (hpf). The results showed that the higher the concentration of [Cn mim]NO3 (n = 2, 4, 6), the shorter the body length and swimming distance, and the longer the immobility time. Besides, a longer alkyl chain length of [Cn mim]NO3 had a more negative effect on body length and locomotor behavior. RNA-seq analysis revealed several downregulated differentially expressed genes (DEGs)-grin1b, prss1, gria3a, and gria4a-enriched in neurodevelopment-related pathways, particularly the pathway for neuroactive ligand-receptor interaction. Moreover, several upregulated DEGs, namely col1a1a, col1a1b, and acta2, were mainly associated with skeletal development. Expression of DEGs was tested by RT-qPCR, and the outcomes were consistent with those obtained from RNA-Seq. We provide evidence showing the effects of parental exposure to ILs on the regulation of nervous and skeletal development in F1 offspring, demonstrating intergenerational effects.

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This study investigated the response of nitrite accumulation to elevated COD/NO3--N ratio (C/N) during partial denitrification (PD). Results indicated nitrite was gradually accumulated and remained stable (C/N = 1.5 âˆ¼ 3.0), while that rapidly declined after reaching the peak (C/N = 4.0 âˆ¼ 5.0). The polysaccharide (PS) and protein (PN) content of tightly-bound extracellular polymeric substances (TB-EPS) reached the maximum at C/N of 2.5 âˆ¼ 3.0, which might be stimulated by high level of nitrite. Illumina MiSeq sequencing showed Thauera and OLB8 were dominated denitrifying genera at C/N of 1.5 âˆ¼ 3.0, while Thauera was further enriched with fading OLB8 at C/N of 4.0 âˆ¼ 5.0. Meanwhile, the highly-enriched Thauera might enhance the activity of nitrite reductase (nirK) promoting further nitrite reduction. Redundancy analysis (RDA) showed positive correlations between nitrite production and PN content of TB-EPS, denitrifying bacteria (Thauera and OLB8) and nitrate reductases (narG/H/I) in low C/N. Finally, their synergistic effects for driving nitrite accumulation were comprehensively elucidated.

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Nitrate (NO3-) is one of the ubiquitous environmental chemicals which multiplies negative impacts on aquatic life such as amphibian larvae. However, the data involving the dynamics of amphibians in response to NO3-N are scarce. This study investigated the effects of NO3-N on locomotor ability, growth performance, oxidative stress parameters, intestinal histology, and intestinal microbiota of Bufo raddei Strauch tadpoles. The tadpoles were chronically exposed to different concentrations of NO3-N (10, 50, 100, and 200 mg/L) from Gosner stage 26 to 38. Our results revealed that NO3-N exposure caused significantly reduced body weight and length, impaired locomotor activity, and severe oxidative damage to liver tissue. Moreover, the high NO3-N (50, 100, and 200 mg/L) exposure caused irregular arrangement and indistinct cell borders of mucosal epithelial cells in the tadpoles intestine. The NO3-N exposure significantly changed the structure of the intestinal microbiota. The phylum Cyanobacteria occupy the main niche of intestinal microbes and have a certain negative correlation with the growth and motility of tadpoles. In addition, the functional prediction revealed that NO3-N exposure obviously downregulated the metabolism of enzyme families in tadpoles. Our comprehensive research shows the toxicity of NO3-N exposure in B. raddei Strauch, explores the potential links between development and intestinal microbiota of tadpole, and provides a new framework for the potential health risk of nitrate in amphibians.

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The sources of P and N in water-carrying lakes include exogenous input and endogenous release. However, the influence of pollution from different sources on the dynamic distribution of N and P at the sediment-water interface in water-carrying lakes remains unclear. The objectives of this study were to investigate the differences in dynamic distribution characteristics of P compounds and N elements in Lake Jiaogang, a major water-carrying lake in eastern China. Four functional regions with different types of pollutant sources and different kinds of aquatic plants were selected to study the distribution of total P (TP), inorganic P, organic P, ammonium (NH4+-N), and nitrate (NO3--N). The results revealed that regions with internal-source pollutants contained the highest concentration of TP, Ca-P, and Fe-P with high concentrations. L-P, Al-P, mostly organic P, and soluble reactive phosphorous (SRP), the region with internal-source pollutants were lower than that with the imported-source pollutant. The concentration of dissolved NH4+-N showed high in regions with imported-source pollutants, however, in regions with internal-source pollutants, the dissolved NO3--N was with the highest concentration. Overall, P from upstream was still dominant in the sediments despite uptake by the aquatic plants. SRP showed high concentration in regions with imported-source pollutants due to the imported pollution and the improved bioavailability by plant root exudates. Feces and feed residues from aquatic livestock breeding resulted in the highest concentration of TN, NH4+-N, and dissolved NO3--N in the sediments of the region with internal-source pollutants. High concentrations of dissolved NH4+-N were due to the input of N from imported source pollutants. This study provides insights into the contributions of P and N to the eutrophication of the water-carrying lake.

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The rhizosphere of invasive plants presumably develops different soil microbial assemblages compared with native plants, which may hinder or promote their invasion. However, to date, no studies have clearly explored rhizosphere microbial community assemblages during invasion. The invasive species Ambrosia artemisiifolia L. and Bidens pilosa L. are widely distributed in China and are known to reduce local biodiversity and cause agricultural losses. Monoculture of A. artemisiifolia or B. pilosa, a mixture of each invasive and native species, and monoculture of native species were established to simulate different degrees of invasion. Metagenomic sequencing techniques were used to test microbial community structure and function. The aim was to explore the drivers of the assembly of peculiar functional microbes in the rhizosphere soil of invasive species during the long-term invasive-native species interaction. Compared with the native species, the relative abundance of 34 microbial genera was higher in the rhizosphere soil of the invasive species. The NO3-N concentration in the rhizosphere soil from the A. artemisiifolia and B. pilosa monocultures was lower than that from monocultures of the three native plants, whereas pH followed the opposite trend. The NO3-N concentration was significantly and negatively correlated with Sporichthya, Afipia, Actinokineospora, and Pseudolabrys. pH was positively correlated with Bradyrhizobium, Actinoplanes, Micromonospora, Steroidobacter, Burkholderia, and Labilithrix. The differences in soil microbes, NO3-N concentrations, and pH between native and invasive species suggest that the rhizosphere soil microbial assemblages may vary. The reduced NO3-N concentration and increased pH corelated with changes in rhizosphere microbial community during A. artemisiifolia and B. pilosa invasion. IMPORTANCE Soil microbial communities play a vital role in the growth of invasive plants. Invasive species may shape peculiar functional microbes in the rhizosphere soil of an invasive species to benefit its growth. However, the drivers of the assembly of soil microbial communities in the rhizosphere soil of invasive species remain unclear. Our study established the relationship between soil microbial communities and soil chemical properties during invasion by A. artemisiifolia and B. pilosa. Additionally, it showed that the presence of the invasive plants correlated with changes in NO3-N and pH, as well as in rhizosphere microbial community assemblage. Furthermore, the study provided important insights into the difference in the microbial community assembly between native and invasive plant species.

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Sustainable field crop management has been considered to reach the food security issue due to global warming and water scarcity. The effect of deficit irrigation and nitrogen rates on quinoa yield is a challenging issue in those areas. In this regard, the interaction effects of different N rates (0, 125, 250, and 375 kg N ha-1) and irrigation regimes [full irrigation (FI) and deficit irrigation at 0.75 FI and 0.5 FI] on quinoa yield and water and nitrogen efficiencies were evaluated with a two-year field experiment. Increasing nitrogen fertilizer application levels from 250 to 375 kg N ha-1 under FI and deficit irrigation did not cause a significant difference in seed yield and the total dry matter of quinoa. Furthermore, 20% and 34% reductions were observed for nitrogen use efficiency (NUE) and nitrogen yield efficiency with the application of 375 kg N ha-1 compared with that obtained in 250 kg N ha-1 nitrogen fertilizer, respectively. Therefore, a Nitrogen application rate of 250 kg ha-1 and applying 0.75 FI is suggested as the optimum rate to reach the highest seed water use efficiency (0.7 kg m-3) and NUE (0.28 kg m-3) to gain 4.12 Mg ha-1 quinoa seed yield. Under non-limited water resource conditions, an FI and N application rate of 375 kg ha-1 could be used for higher seed yield; however, under water-deficit regimes, an N application rate of 250 kg ha-1 could be adequate. However, questions about which environmental factors impressively restricted the quinoa growth for optimizing the potential yield need further investigation.

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Nutrient management, as described in NRCS Code 590, has been intensively investigated, with research largely focused on crop yields and water quality. Yet, due to complex processes and mechanisms in nutrient cycling (especially the nitrogen (N) cycle), there are many challenges in evaluating the effectiveness of nutrient management practices across site conditions. We therefore synthesized data from peer-reviewed publications on subsurface-drained agricultural fields in the Midwest U.S. with corn yield and drainage nitrate-N (NO3-N) export data published from 1980 to 2019. Through literature screening and data extraction from 43 publications, we obtained 577 site-years of data with detailed information on fertilization, corn yields, precipitation, drainage volume, and drainage NO3-N load/concentration or both. In addition, we estimated flow-weighted NO3-N concentrations ([NO3-N]) in drainage for those site-years where only load and volume were reported. Furthermore, we conducted a cost analysis using synthesized and surveyed corn yield data to evaluate the cost-effectiveness of different nutrient management plans. Results from the synthesis showed that N fertilizer rate was strongly positively correlated with corn yields, NO3-N loads, and flow-weighted [NO3-N]. Reducing N fertilizer rates can effectively mitigate NO3-N losses from agricultural fields; however, our cost analysis showed negative economic returns for continuous corn production at lower N rates. In addition, organic fertilizers significantly boosted corn yields and NO3-N losses compared to inorganic fertilizers at comparable rates; however, accurate quantification of plant-available N in organic fertilizers is necessary to guide appropriate nutrient management plans because the nutrient content may be highly variable. In terms of fertilizer application methods, we did not find significant differences in NO3-N export in drainage discharge. Lastly, impact of fertilization timing on NO3-N export varied depending on other factors such as fertilizer rate, source, and weather. According to these results, we suggest that further efforts are still required to produce effective local nutrient management plans. Furthermore, government agencies such as USDA-NRCS need to work with other agencies such as USEPA to address the potential economic losses due to implementation of lower fertilizer rates for water quality improvement.

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To increase crops yields, applying large amounts of fertilizers has become increasingly common in agricultural regions, resulting in NO3--N groundwater pollution. Agricultural non-point pollution is the main source of groundwater NO3--N pollution. To ensure drinking water safety and quality, it is crucial to clarify the sources of NO3--N pollution in agricultural regions. In this study, 35 sampling sites were randomly selected in the Qingdao agricultural area in 2009 and 2019. The spatial distribution of NO3--N concentration was analyzed by the inverse distance weighting method (IDW). The nitrogen and oxygen isotopes were used as a tool to trace sources of NO3--N and the SIAR model was used to quantify contribution proportion of pollution sources. The results showed that the concentration of NO3--N (average) in groundwater in Qingdao has been reduced from 38.49 mg·L-1 in 2009 to 22.37 mg·L-1 in 2019, but it is still higher than the maximum allowable concentration of NO3--N in drinking water set by the World Health Organization (WHO). The NO3--N concentration gradually increased from south to north both in 2009 and 2019. The cross diagram of δ15N-NO3- and δ18O-NO3- show that the main sources of NO3--N in groundwater in Qingdao are chemical fertilizers, soil nitrogen, and manure and sewage. Water isotopes indicate that precipitation was the main source of groundwater in Qingdao. The SIAR model results indicated that the contribution of each source ranked as follows:manure and sewage (47.42%) > soil nitrogen (27.80%) > chemical fertilizer (14.32%) > atmospheric nitrogen depositions (10.43%). From 2009 to 2019, the quality of groundwater in Qingdao has been improved, but NO3--N pollution still cannot be ignored. According to the results, prevention and control should be made to ensure the safety of drinking water and the sustainable development of agriculture.

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The effects of biochar and straw return on soil N2O emissions were studied in the winter wheat-summer maize rotation system of intensively farmed land in North China to provide a theoretical basis for N2O emission reduction and the efficient straw utilization. The experiment included the following four treatments:① Control (CK); ② Biochar application at a rate of 9.0 t·(hm2·a)-1 (C); ③ Straw return (SR); and ④ Straw return plus biochar application at a rate of 9.0 t·(hm2·a)-1 (C+SR). The results showed that in the wheat season, the CK treatment showed a slight decrease in soil N2O emission while the SR and C+SR treatments promoted soil N2O emission by 47.4% and 71.8%, respectively. In the maize-growing season, the CK treatment reduced soil N2O emission by 29.8% while the SR and C+SR treatments increased soil N2O emission by 13.4% and 35.8%, respectively. During the wheat-growing season, the soil water, NH4+-N, and MBN content were the main environmental factors affecting N2O emissions; during the maize-growing season, NO3--N, NH4+-N, and MBC content were the main environmental factors affecting emissions. Based on our results, the application of biochar to cropland is an effective option for mitigating greenhouse gas emissions, whereas direct straw return to fields might not be an effective strategy. More research is now needed to examine the effect of the return of straw of different maturity on N2O emissions.

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In order to enhance nitrogen removal through anammox process in the full-scale swine wastewater treatment plant, an innovative regulation strategy of nitrate-based carbon dosage and intermittent aeration was developed to apply the combined biological nitrogen removal process in a full scale anaerobic-anoxic-oxic (A2/O) system. TN removal efficiency reached at 65.5 ± 6.0% in Phase 1 with decreasing external carbon dosage in influent due to the reduction of return nitrate concentration, and it increased to 83.5 ± 6.7% when intermittent aeration was adopted in oxic zone and external carbon source was stopped adding into influent in Phase 2. As a result, the energy consumption for the swine wastewater treatment decreased from 1.93 to 0.9 kW h/m3 and 4.18 to 2.57 kW h/kg N, respectively. Microbial community analysis revealed that the average abundances of Candidatus Brocadia increased from 0.76% to 2.43% and removal of TN through anammox increased from 39% to 77%.

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Combining organic fertilizer with chemical fertilizer may affect the microbial processes related to nitrous oxide (N2O) emissions under different degrees of soil salinization. A mild saline soil (S1; electrical conductivity (EC) 0.46 dS·m-1) and moderate saline soil (S2; EC 1.07 dS·m-1) in the Hetao irrigation district of Inner Mongolia were selected. Under equal N rates, the study involved five treatments:U1 (240 kg·hm-2 of chemical fertilizer), U3O1 (180 kg·hm-2 of chemical fertilizer+60 kg·hm-2 of organic fertilizer), U1O1 (120 kg·hm-2 of chemical fertilizer+120 kg·hm-2 of organic fertilizer), U1O3 (60 kg·hm-2 of chemical fertilizer+180 kg·hm-2 of organic fertilizer), and O1 (240 kg·hm-2 of organic fertilizer). In addition, a blank control treatment (CK) was employed to investigate the effects of different fertilization treatments on the N2O emissions from the two saline soils. The results showed that the total N2O emissions from the S2 soil in the same treatment were between 11.86% and 47.23% higher than those from the S1 soil (P<0.05). the dynamic trend of the soil N2O fluxes among the different treatments were similar. The peak N2O emissions occurred after fertilization, and the N2O cumulative emission fluxes accounted for nearly 60% of the emissions during the entire growth period. The proper application of organic fertilizer could significantly reduce the soil N2O emission; the S1 and S2 saline soils treated with U1O1 and O1, respectively, had the lowest N2O emissions, whereby there were significant reductions of 33.62% and 28.51% in comparison to the U1 treatment (P<0.05). Moreover, higher maize yields could be obtained and the N2O flux was significantly positively correlated with the NH4+-N content (P<0.01) but negatively correlated with the NO3--N content. The results demonstrated that nitrification was the main way to produce N2O in the studied salinized maize farmland, and that the addition of organic fertilizer could reduce the N2O production by decreasing the soil NH4+-N content. Based on the changes in the corn yield and a reduction in the greenhouse effect, suitable organic and inorganic fertilizer management models for the Hetao irrigation area were the mild saline soil:120 kg·hm-2 of urea +120 kg·hm-2 of organic fertilizer), and the moderate saline soil:240 kg·hm-2 of organic fertilizer.

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The use of microbial photoelectrochemical cells (MPECs) for the removal of contaminants is a cost-effective and environment-friendly method. Based on the preparation of polyaniline/titanium dioxide nanotube array (PANI/TiO2-NTs) composite photoelectrodes, an MPEC system comprising PANI/TiO2-NTs photoanode and biocathode was constructed and the removal performance of nitrate nitrogen (NO3--N) was studied. The experimental results showed that the PANI/TiO2-NT electrode exhibited the best photoelectric performance when the PANI loading time was 80 s. Compared with the TiO2-NTs electrode, the photocurrent density doubled. The light-driven MPEC system could realize autotrophic denitrification without an external voltage. The biodegradation of NO3--N conformed to the pseudo first-order kinetics. The higher the photoresponse current density, the better the denitrification performance of the system. When the initial concentration of NO3--N was 25 mg·L-1 and the photoresponse current density increased from 0.17 mA·cm-2 to 0.67 mA·cm-2, the average denitrification rate increased from 0.83 mg·(L·h)-1 to 2.83 mg·(L·h)-1. High-throughput sequencing of the biocathode microbial membranes revealed that Pseudomonas (27.37%) was the dominant bacteria. It was considered that the photogenerated electrons generated by the PANI/TiO2-NTs photoanode were transmitted to the cathode through an external circuit. Pseudomonas and other microorganisms with autotrophic denitrification and electrochemical activity directly used the electrons on the electrode as the sole electron donors for autotrophic denitrification reaction.

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The effect of hydraulic retention time (HRT) on denitrifying phosphorus and nitrogen removal in a modified two sludge A2/O-BAF system was studied. The influent COD, NH4+-N, and TP were 189.6, 60.4, and 5.1 mg·L-1, respectively. When HRT was 9, 8, 7, and 6 h, the average effluent COD was less than 42 mg·L-1. The average effluent NH4+-N levels were 2.4, 2.8, 3.3, and 6.5 mg·L-1, respectively. The average effluent TP values were 0.3, 0.4, 0.7, and 0.8 mg·L-1, respectively. The ratio of the denitrifying phosphorus accumulation organisms (DPAOs/PAOs) in the system of anoxic zone was reduced from 76.8% to 48.8%. When HRT was 8 h, the ratio of denitrifying phosphorus to nitrogen (ΔPO43-/ΔNO3--N) was increased by 37.5% by a mathematical statistics method. The ΔPO43-/ΔNO3--N in the anoxic zone was 1.24 (the theoretical value is 1.41). At this time, the effect of denitrifying phosphorus to nitrogen was the best. The SVI value was lower than 100 mL·g-1 throughout the experiment, and the MLVSS/MLSS gradually decreased from 0.74 to 0.63, indicating that the sludge activity was reduced.