RESUMEN
Hepatitis E virus (HEV) infection is considered a neglected disease of major concern in developed countries. Clinically, HEV occurs as an acute and self-limited disease, though chronic cases mostly associated to HEV-3 are now being commonly reported in immunocompromised individuals and solid organ transplant recipients. Transmission of HEV through blood and derivatives have been increasingly described in the last years, highlighting the importance of including this agent on the screening programs. Since 2010 both acute and chronic hepatitis E cases have been frequently reported in Uruguay. However, updated prevalence data among different population groups are lacking and HEV is not currently screened in blood banks. Herein, we report a seroprevalence and molecular survey of HEV in 400 plasma samples from blood donors. Overall, our results showed an HEV seroprevalence rate of 10% (40/400); almost 10-fold higher than 20 years ago. Total anti-HEV immunoglobulin antibodies were found to increase with age. Moreover, we reported an RNA detection rate of at least 0.75%, and two strains were sequenced. Phylogenetic analysis grouped them with human and swine HEV-3 strains from Uruguay. Data presented here should prompt public health policies of HEV screening in blood banks to minimize the risk of transfusion-transmitted hepatitis E.
Asunto(s)
Donantes de Sangre , Virus de la Hepatitis E/genética , Virus de la Hepatitis E/inmunología , Hepatitis E/epidemiología , Hepatitis E/inmunología , Adolescente , Adulto , Factores de Edad , Donantes de Sangre/estadística & datos numéricos , Transfusión Sanguínea , Anticuerpos Antihepatitis/sangre , Hepatitis E/sangre , Hepatitis E/transmisión , Humanos , Persona de Mediana Edad , Filogenia , Prevalencia , ARN Viral/sangre , ARN Viral/genética , Estudios Seroepidemiológicos , Receptores de Trasplantes/estadística & datos numéricos , Uruguay/epidemiología , Adulto JovenRESUMEN
El virus papiloma humano o VPH representa una de las enfermedades de transmisión sexual más frecuente, principal causa de cáncer cervical. Objetivo. Determinar la prevalencia de las infecciones genitales por VPH, identificar factores clínico-epidemiológicos asociados a dicha prevalencia y obtener la frecuencia de los tipos virales, en mujeres universitarias sintomáticas y asintomáticas en Ecuador, año 2020. Metodología. Investigación descriptiva, transversal, experimental de campo, la población estuvo constituida por 135 estudiantes con edad comprendida entre 18-28 años. Como instrumento de recolección de datos se realizó una encuesta sobre factores clínicos, la detección del VPH fue por reacción en cadena de la polimerasa (PCR) amplificándose una sección de 450 pares de bases del genoma viral perteneciente al fragmento L1, la detección del genotipo de VPH se realizó mediante el estudio del polimorfismo de la longitud de los fragmentos de restricción (RFLP). Resultado. Se encontró una prevalencia de VPH de 15,56% (21/135), la edad la mayor prevalencia se encontró en mujeres de 24-28 años (57,14%), 19,05% (4/21) de las mujeres que habían tenido embarazos salieron VPH (+), referente a los síntomas, 60,00% era sintomáticas y 40,00% asintomáticas, de las cuales 20,37 (11/54) resultaron VPH (+). En 66,67 % (8/12) se detectó secuencias de ADN de VPH de alto riesgo. El conocimiento integrado de todos estos factores o elementos que involucran al paciente como entidad social y biológica, al virus y a los posibles co-factores es fundamental para la implementación de programas de prevención (educativos y profilácticos) y para el óptimo manejo de los recursos disponibles en el tratamiento y seguimiento de los distintos niveles de esta patología(AU)
The human papillomavirus or HPV represents one of the most common sexually transmitted diseases, the main cause of cervical cancer. Objective. Determine the prevalence of genital HPV infections, identify clinical-epidemiological factors associated with said prevalence and obtain the frequency of viral types in symptomatic and asymptomatic women university in Ecuador, year 2020. Methodology. Descriptive, cross-sectional, experimental field research, the population consisted of 155 students aged between 18-28 years. As a data collection instrument, a survey on clinical factors was carried out, the detection of HPV was by polymerase chain reaction (PCR), amplifying a section of 450 base pairs of the viral genome belonging to the L1 fragment, the detection of the genotype of HPV was performed by studying the restriction fragment length polymorphism (RFLP). Outcome. An HPV prevalence of 15.56% (21/135) was found, the age the highest prevalence was found in women 24-28 years (57.14%), 19.05% (4/21) of women who had pregnancies came out HPV (+), referring to symptoms, 60.00% were symptomatic and 40.00% asymptomatic, of which 20.37 (11/54) were HPV (+). High-risk HPV DNA sequences were detected in 66.67% (8/12). Integrated knowledge of all these factors or elements that involve the patient as a social and biological entity, the virus and possible co-factors is essential for the implementation of prevention programs (educational and prophylactic) and for the optimal management of available resources in the treatment and monitoring of the different levels of this pathology
Asunto(s)
Humanos , Femenino , Adolescente , Adulto , PapillomaviridaeRESUMEN
Bovine anaplasmosis, caused by the tick-borne pathogen Anaplasma marginale, is a hemolytic disease that constitutes a major constraint to cattle production in tropical and subtropical regions including Ecuador. However, the epidemiological situation of this hemoparasitosis in Ecuador is poorly characterized. The present study was aimed to determine the prevalence and genetic diversity of A. marginale in cattle of Ecuador. A cross-sectional study was carried out covering several farms from six out nine cantons of the Zamora-Chinchipe province. A total of 185 cattle were randomly selected and blood samples were collected from the animals. The studied group of animals included six breeds, three age groups, and both sexes. The molecular diagnostic was performed based on a nPCR assay targeting the A. marginale msp5 gene. Anaplasma marginale prevalence was 63.8 % and the bacteria were detected in all the cantons studied. Thirteen representative strains were selected and genetically characterized based on the msp1α gene. Genetic diversity analysis revealed that different strains circulate in the bovine herds studied. The results suggest that cattle movement may contribute to the circulation of common strains in the area. The results demonstrate a high prevalence of A. marginale in the region which should be considered by the sanitary authorities. The epidemiological surveillance for this disease should increase to anticipate acute disease outbreaks with high mortality. Bovine anaplasmosis outbreaks can cause economic losses and the death of several animals; therefore, measures for the prevention and control of this disease are required.