RESUMEN

This protocol describes the genomic phage (gPhage) display platform, a large-scale antigen and epitope mapping technique. We constructed a gPhage display peptide library of a eukaryotic organism, Trypanosoma cruzi (causative agent of Chagas disease), to map the antibody response landscape against the parasite. Here, we used an organism with a relatively large but intronless genome, although future applications could include other prevalent or (re)emerging infectious organisms carrying genomes with a limited number of introns. For complete details on the use and execution of this protocol, please refer to Teixeira et al. (2021).

Asunto(s)

Técnicas de Visualización de Superficie Celular/métodos , Biblioteca Genómica , Anticuerpos Antiprotozoarios/química , Anticuerpos Antiprotozoarios/metabolismo , Genoma de Protozoos/genética , Trypanosoma cruzi/genética