RESUMEN
This study aims to evaluate the wound healing potential of lectin isolated from the seeds of Centrolobium microchaete (Mart. ex Benth) (CML) on cutaneous wounds in mice. CML did not show cytotoxicity on murine dermal fibroblasts (L929 cell line). The wounds treated with CML (200 µg/mL) showed a decrease in area within 12 days post-operative (P.O.) when compared to control. On 3rd and 7th day P.O., the CML-treated group exhibited fibroblast proliferation and neovascularization. On 12th day P.O., complete restructuring of the epithelial layer and connective tissue was observed in the CML-treated group, whereas control groups exhibited incomplete reepithelialization. CML treatment enhanced the wound closure via the wound contraction process, resulting in the restructuring of the skin layers on 12th day P.O. In conclusion, CML induced a fast and efficient wound healing, suggesting that it can be used as a promising therapeutic tool to heal acute wounds.
Asunto(s)
Fabaceae , Lectinas , Animales , Lectinas/farmacología , Ratones , Semillas , Piel , Cicatrización de HeridasRESUMEN
With the rapid development of nanotechnology, much has been anticipated with silver nanoparticles (AgNPs) due to their extensive industrial and commercial applications. However, it has raised concerns over environmental safety and human health effects. In this study, AgNPs were bio-fabricated using aqueous extract of Microchaete and their medical applications like antioxidant, anti-proliferative, and apoptosis were done. The biosynthesis of AgNPs was continuously followed by UV-vis spectrophotometric analysis. The physiochemical properties like shape, size, crystallinity, and polydispersity of the nanoparticles were determined by Scanning Electron Microscopy (SEM) along with EDX, Transmission Electron Microscope (TEM), Atomic Force Microscope (AFM), dynamic light scattering (DLS), and X-Ray Diffraction (XRD). Biosynthesized 7.0 nm sized AgNPs with the crystalline structure (crystalline size 4.8 nm) having a hydrodynamic diameter of 38.74 ± 2.6 nm was achieved due to the involvement of reducing agents present in the cyanobacterial extract. The IC50 values of the AgNPs were evaluated as 75 µg/ml and 79.41 µg/ml with HepG2 and MCF-7 cell lines. Different in-vitro cellular assays investigated in the present study exhibited antioxidant, anti-proliferative, and apoptotic activities. Probably delayed apoptosis in HepG2 and MCF-7 is due to better antioxidant activities of Microchaete based AgNPs.
Asunto(s)
Antineoplásicos/farmacología , Antioxidantes/química , Apoptosis/efectos de los fármacos , Nanopartículas del Metal/química , Plata/química , Antineoplásicos/síntesis química , Antineoplásicos/química , Línea Celular Tumoral , Cianobacterias/química , Cianobacterias/metabolismo , Tecnología Química Verde , Humanos , Peróxido de Hidrógeno/química , Nanopartículas del Metal/toxicidad , Estrés Oxidativo/efectos de los fármacosRESUMEN
The green synthesis of metallic nanoparticles has paved the way for improving and protecting the environment by decreasing the use of toxic chemicals and eliminating biological risks in biomedical applications. Biological synthesis of metal nanoparticles is gaining more importance due to simplicity, rapid rate of synthesis and eco-friendliness. In the present investigation cyanobacterial (Microchaete NCCU-342) cell free aqueous extract has been used for optimizing biosynthesis of silver nanoparticles (AgNP). The optimized reaction parameters for efficient synthesis of AgNP were: biomass quantity of 80⯵g/ml, pHâ¯5.5, 60⯰C temperature, duration of 60â¯min UV light exposure and 1â¯mM AgNO3 concentration. AgNP was characterized by UV-Visible Spectrophotometery, Transmission Electron Microscopy and Dynamic light scattering. The smallest nanoparticles (obtained from biomass parameter were spherical, polydisperessed and in the range of 60-80â¯nm) were characterized further in a degradation study of azo dye methyl red. Degradation of methyl red within 2â¯h was more with AgNP (84.60%) as compared to cyanobacterial extract (49.80%).
Asunto(s)
Cianobacterias/metabolismo , Nanopartículas del Metal/química , Nanotecnología/métodos , Plata , Catálisis , Tamaño de la PartículaRESUMEN
This study aimed to purify and characterize a novel mannose-binding lectin from the seeds of Centrolobium microchaete. Centrolobium microchaete lectin (CML) was purified by affinity chromatography in mannose-Sepharose-4B column. CML agglutinated rabbit erythrocytes and was inhibited by D-mannose, α-methyl-D-mannoside, D-glucose, N-Acetyl-D-glucosamine and sucrose. The lectin was stable at pH 7.0 and 8.0 and temperatures up to 60°C. The monomeric form of CML showed approximately 28kDa, and its native form is probably a homodimer, as determined by gel filtration chromatography. The primary structure of CML was determined by tandem mass spectrometry that showed CML as a protein with two distinct forms (isolectins CML-1 and CML-2) with 246 and 247 residues, respectively. CML-2 possesses one residue of Asn more than CML-1 in C-terminal. The primary structure of CML agrees with the molecular weights found by electrospray ionization mass spectrometry: 27,224 and 27,338Da for CML-1 and CML-2, respectively. CML is a metal-dependent glycoprotein. Moreover, the glycan composition of CML and its structure were predicted.