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The slow stabilization process of landfill had brought obstacles to urbanization. The paper investigated the efficacy and mechanism of micro-aeration intensity for landfill stabilization. The micro-aeration intensity of 0.05 L/(h·kg) resulted in a significant increase of volatile fatty acids (VFAs) in the hydrolysis stage, and the NH4+-N concentration was reduced by 22.1 %. At the end of landfill, VFAs were rapidly degraded and organic matter was reduced from 36 % to 16 %, which was 55.5 % more efficient than the control group. In addition, the community succession and structure of bacteria and archaea were analyzed. The micro-aeration intensity of 0.05 L/(h·kg) increased the abundance of hydrolyzing functional bacteria such as Pseudomonas and Bacillus, and allowed methanogenic bacteria such as Methanobacterium and Methanothrix to gradually establish oxygen tolerance in the microaerobic environment. The appropriate micro-aeration intensity can accelerate the stabilization process of landfill, which has environmental and economic benefits.

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To effectively treat actual ammonia-rich Chinese medicine residue (CMR) resource utilization wastewater, we optimized an anaerobic-microaerobic two-stage expanded granular sludge bed (EGSB) and moving bed sequencing batch reactor (MBSBR) combined process. By controlling dissolved oxygen (DO) levels, impressive removal efficiencies were achieved. Microaeration, contrasting with anaerobic conditions, bolstered dehydrogenase activity, enhanced electron transfer, and enriched the functional microorganism community. The increased relative abundance of Synergistetes and Proteobacteria facilitated hydrolytic acidification and fostered nitrogen and phosphorus removal. Furthermore, we examined the impact of DO concentration in MBSBR on pollutant removal and microbial metabolic activity, pinpointing 2.5 mg/L as the optimal DO concentration for superior removal performance and energy conservation.

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Biotechnology for wastewater treatment is mainstream and effective depending upon microbial redox reactions to eliminate diverse contaminants and ensure aquatic ecological health. However, refractory organic nitrogen compounds (RONCs, e.g., nitro-, azo-, amide-, and N-heterocyclic compounds) with complex structures and high toxicity inhibit microbial metabolic activity and limit the transformation of organic nitrogen to inorganic nitrogen. This will eventually result in non-compliance with nitrogen discharge standards. Numerous efforts suggested that applying exogenous electron donors or acceptors, such as solid electrodes (electrostimulation) and limited oxygen (micro-aeration), could potentially regulate microbial redox reactions and catabolic pathways, and facilitate the biotransformation of RONCs. This review provides comprehensive insights into the microbial regulation mechanisms and applications of electrostimulation and micro-aeration strategies to accelerate the biotransformation of RONCs to organic amine (amination) and inorganic ammonia (ammonification), respectively. Furthermore, a promising approach involving in-situ hybrid anaerobic biological units, coupled with electrostimulation and micro-aeration, is proposed towards engineering applications. Finally, employing cutting-edge methods including multi-omics analysis, data science driven machine learning, technology-economic analysis, and life-cycle assessment would contribute to optimizing the process design and engineering implementation. This review offers a fundamental understanding and inspiration for novel research in the enhanced biotechnology towards RONCs elimination.

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This research aims to conduct a comparative investigation of the role played by microaeration and sludge recirculation in the novel anaerobic baffled biofilm-membrane bioreactor (AnBB-MBR) for enhancing pharmaceutical removal from building wastewater. Three AnBB-MBRs - R1: AnBB-MBR, R2: AnBB-MBR with microaeration and R3: AnBB-MBR with microaeration and sludge recirculation - were operated simultaneously to remove Ciprofloxacin (CIP), Caffeine (CAF), Sulfamethoxazole (SMX) and Diclofenac (DCF) from real building wastewater at the hydraulic retention time (HRT) of 30 h for 115 days. From the removal profiles of the targeted pharmaceuticals in the AnBB-MBRs, it was found that the fixed-film compartment (C1) could significantly reduce the targeted pharmaceuticals. The remaining pharmaceuticals were further removed with the microaeration compartment. R2 exhibited the utmost removal efficiency for CIP (78.0 %) and DCF (40.8 %), while SMX was removed most successfully by R3 (microaeration with sludge recirculation) at 91.3 %, followed by microaeration in R2 (88.5 %). For CAF, it was easily removed by all AnBB-MBR systems (>90 %). The removal mechanisms indicate that the microaeration in R2 facilitated the adsorption of CIP onto microaerobic biomass, while the enhanced biodegradation of CAF, SMX and DCF was confirmed by batch biotransformation kinetics and the adsorption isotherms of the targeted pharmaceuticals. The microbial groups involved in biodegradation of the targeted compounds under microaeration were identified as nitrogen removal microbials (Nitrosomonas, Nitrospira, Thiobacillus, and Denitratisoma) and methanotrophs (Methylosarcina, Methylocaldum, and Methylocystis). Overall, explication of the integration of AnBB-MBR with microaeration (R2) confirmed it as a prospective technology for pharmaceutical removal from building wastewater due to its energy-efficient approach characterized by minimal aeration supply.

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Micro-aeration was shown to improve anaerobic digestion (AD) processes, although oxygen is known to inhibit obligate anaerobes, such as syntrophic communities of bacteria and methanogens. The effect of micro-aeration on the activity and microbial interaction in syntrophic communities, as well as on the potential establishment of synergetic relationships with facultative anaerobic bacteria (FAB) or aerobic bacteria (AB), was investigated. Anaerobic sludge was incubated with ethanol and increasing oxygen concentrations (0-5% in the headspace). Assays with acetate or H2/CO2 (direct substrates for methanogens) were also performed. When compared with the controls (0% O2), oxygen significantly decreased substrate consumption and initial methane production rate (MPR) from acetate or H2/CO2. At 0.5% O2, MPR from these substrates was inhibited 30-40%, and close to 100% at 5% O2. With ethanol, significant inhibition (>36%) was only observed for oxygen concentrations higher than 2.5%. Oxygen was consumed in the assays, pointing to the stimulation of AB/FAB by ethanol, which helped to protect the syntrophic consortia under micro-aerobic conditions. This highlights the importance of AB/FAB in maintaining functional and resilient syntrophic communities, which is relevant for real AD systems (in which vestigial O2 amounts are frequently present), as well as for AD systems using micro-aeration as a process strategy. KEY POINTS: •Micro-aeration impacts syntrophic communities of bacteria and methanogens. •Oxygen stimulates AB/FAB, maintaining functional and resilient consortia. •Micro-aeration studies are critical for systems using micro-aeration as a process strategy.

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Biogas is produced by a consortium of bacteria and archaea. We studied how the microbiome of poultry litter digestate was affected by time and treatments that enhanced biogas production. The microbiome was analyzed at six, 23, and 42 weeks of incubation. Starting at week seven, the digesters underwent four treatments: control, microaeration with 6 mL air L-1 digestate per day, treatment with a 1000 Hz sine wave, or treatment with the sound wave and microaeration. Both microaeration and sound enhanced biogas production relative to the control, while their combination was not as effective as microaeration alone. At week six, over 80% of the microbiome of the four digesters was composed of the three phyla Actinobacteria, Proteobacteria, and Firmicutes, with less than 10% Euryarchaeota and Bacteroidetes. At week 23, the digester microbiomes were more diverse with the phyla Spirochaetes, Synergistetes, and Verrucomicrobia increasing in proportion and the abundance of Actinobacteria decreasing. At week 42, Firmicutes, Bacteroidetes, Euryarchaeota, and Actinobacteria were the most dominant phyla, comprising 27.8%, 21.4%, 17.6%, and 12.3% of the microbiome. Other than the relative proportions of Firmicutes being increased and proportions of Bacteroidetes being decreased by the treatments, no systematic shifts in the microbiomes were observed due to treatment. Rather, microbial diversity was enhanced relative to the control. Given that both air and sound treatment increased biogas production, it is likely that they improved poultry litter breakdown to promote microbial growth.

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Developing efficient landfill leachate treatment is still necessary to reduce environmental risks. However, nitrogen removal in biological treatment systems is often poor or costly. Studying biofilms in anoxic/aerobic zones of rotating biological contactors (RBC) can elucidate how microbial interactions confer resistance to shock loads and toxic substances in leachate treatment. This study assessed the nitritation-anammox performance in an intermittent-rotating bench-scale RBC treating mature leachate (diluted). Despite the leachate toxicity, the system achieved nitritation with an efficiency of up to 34 % under DO values between 0.8 and 1.8 mg.L-1. The highest average ammoniacal nitrogen removal was 45.3 % with 10 h of HRT. The 16S rRNA sequencing confirmed the presence of Nitrosonomas, Aquamicrobium, Gemmata, and Plantomyces. The coexistence of these bacteria corroborated the selective pressure exerted by leachate in the community structure. The microbial interactions found here highlight the potential application of RBC to remove nitrogen in landfill leachate treatment.

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A novel anaerobic baffled biofilm-membrane bioreactor (AnBB-MBR) with microaeration of 0.62 LO2/LFeed was developed to improve VFA and nitrogen removal from building wastewater. Three different membrane bioreactor systems - R1: AnBB-MBR (without microaeration); R2: AnBB-MBR with microaeration; and R3: AnBB-MBR with integrated microaeration and sludge recirculation - were operated in parallel at the same hydraulic retention time of 20 h and sludge retention time of 100 d. The microaeration promoted greater microbial richness and diversity, which could significantly enhance the removal of acetic acid and dissolved methane in the R2 and R3 systems. Moreover, the partial nitrification and the ability of anammox (Candidatus Brocadia) to thrive in R2 enabled NH4+-N removal to be enhanced by up to 57.8 %. The worst membrane fouling was found in R1 due to high amount of protein as well as fine particles (0.5-5.0 µm) acting as foulants that contributed to pore blocking. While the integration of sludge recirculation with microaeration in R3 was able to improve the membrane permeate flux slightly as compared to R2. Therefore, the AnBB-MBR integrated with a microaeration system (R2) can be considered as promising technology for building wastewater treatment when considering VFA and nutrient removal and an energy-saving approach with low aeration intensity.

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Nanaerobes are a newly described class of microorganisms that use a unique cytochrome bd oxidase to achieve nanaerobic respiration at <2 µM dissolved oxygen (∼1% of atmospheric oxygen) but are not viable above this value due to the lack of other terminal oxidases. Although sharing an overlapping ecological niche with methanogenic archaea, the role of nanaerobes in methanogenic systems has not been studied so far. To explore their occurrence and significance, we re-analyzed published meta-omic datasets from animal rumina and waste-to-energy digesters, including conventional anaerobic digesters and anaerobic digesters with ultra-low oxygenation. Results show that animal rumina share broad similarities in the microbial community and system performance with oxygenated digesters, rather than with conventional anaerobic digesters, implying that trace levels of oxygen drive the efficient digestion in ruminants. The rumen system serves as an ideal model for the newly named nanaerobic digestion, as it relies on the synergistic co-occurrence of nanaerobes and methanogens for methane yield enhancement. The most abundant ruminal bacterial family Prevotellaceae contains many nanaerobes, which perform not only anaerobic fermentation but also nanaerobic respiration using cytochrome bd oxidase. These nanaerobes generally accompany hydrogenotrophic methanogens to constitute a thermodynamically and physiologically consistent framework for efficient methane generation. Our findings provide new insights into ruminal methane emissions and strategies to enhance methane generation from biomass.

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The long duration of landfill stabilization is one of the challenges faced by municipalities. In this paper, a combination of micro-aeration and leachate recirculation is used to achieve rapid degradation of organic matter in landfill waste. The results showed that the content of volatile fatty acids (VFAs) in the hydrolysis phase increased significantly and could enter the methanogenic phase quickly. Until the end of the landfill, the removal rates of chemical oxygen demand (COD), total phosphorus (TP) and ammonia nitrogen (NH4+-N) by micro-aeration and leachate recirculation reached 80.17 %, 48.30 % and 48.56 %, respectively, and the organic matter degradation rate reached 50 %. Micro-aeration and leachate recirculation enhanced the abundance of facultative hydrolytic bacteria such as Rummeliibacillus and Bacillus and the oxygen tolerance of Methanobrevibacter and Methanoculleus. Micro-aeration and leachate recirculation improved the organic matter degradation efficiency of landfill waste by promoting the growth of functional microorganisms.

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The effects of microbial electrolysis cells (MECs) at three applied voltages (0.8, 1.3, and 1.6 V) on simultaneously enhancing methanization and reducing hydrogen sulfide (H2S) production in the anaerobic digestion (AD) of sewage sludge were studied. The results showed that the MECs at 1.3 V and 1.6 V simultaneously enhanced the methane production by 57.02 and 12.70% and organic matter removal by 38.77 and 11.13%, and reduced H2S production by 94.8 and 98.2%, respectively. MECs at 1.3 V and 1.6 V created a micro-aerobic conditions for the digesters with oxidation-reduction potential as -178∼-232 mv, which enhanced methanization and reduced H2S production. Sulfur reduction, H2S and elemental sulfur oxidation occurred simultaneously in the ADs at 1.3 V and 1.6 V. The relative abundances of sulfur-oxidizing bacteria increased from 0.11% to 0.42% and those of sulfur-reducing bacteria decreased from 1.24% to 0.33% when the applied voltage of MEC increased from 0 V to 1.6 V. Hydrogen produced by electrolysis enhanced the abundance of Methanobacterium and changed the methanogenesis pathway.

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With the accelerated construction of biogas plants, the amount of biogas residues are expanding. Composting has been widely implemented to deal with biogas residues. Aeration regulation is the main factor affecting the post-composting treatment of biogas residues as high-quality fertilizer or soil amendment. Therefore, this study aimed to investigate the impact of different aeration regulations on full-scale biogas residues compost maturity by controlling oxygen concentration under micro-aeration and aeration conditions. Results showed that micro-aerobic extended the thermophilic stage of 17 days at above 55 ℃ and facilitated the mineralization process of organic nitrogen into nitrate nitrogen to retain higher N nutrition levels compared to aerobic treatment. For biogas residues with high moisture, aeration should be regulated at different full-scale composting stages. Total organic carbon (TOC), NH4+-N, NO3--N, total potassium (TK), total phosphorus (TP) and the germination index (GI) could be used to evaluate stabilization, fertilizer efficiency and phytotoxicity of compost with frequent monitoring times. However, seedling growth trials were still necessary in full-scale composting plants when changing of composting process or biogas residues feedstock.

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Pretreatment of waste before anaerobic digestion (AD) has been extensively studied during the last decades. One of the biological pretreatments studied is the microaeration. This review examines this process, including parameters and applications to different substrates at the lab, pilot and industrial scales, to guide further improvement in large-scale applications. The underlying mechanisms of accelerating hydrolysis and its effects on microbial diversity and enzymatic production were reviewed. In addition, modelling of the process and energetic and financial analysis is presented, showing that microaerobic pretreatment is commercially attractive under certain conditions. Finally, challenges and future perspectives were also highlighted to promote the development of microaeration as a pretreatment before AD.

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Improvement of refractory nitrogen-containing organics biodegradation is crucial to meet discharged nitrogen standards and guarantee aquatic ecology safety. Although electrostimulation accelerates organic nitrogen pollutants amination, it remains uncertain how to strengthen ammonification of the amination products. This study demonstrated that ammonification was remarkably facilitated under micro-aerobic conditions through the degradation of aniline, an amination product of nitrobenzene, using an electrogenic respiration system. The microbial catabolism and ammonification were significantly enhanced by exposing the bioanode to air. Based on 16S rRNA gene sequencing and GeoChip analysis, our results indicated that aerobic aniline degraders and electroactive bacteria were enriched in suspension and inner electrode biofilm, respectively. The suspension community had a significantly higher relative abundance of catechol dioxygenase genes contributing to aerobic aniline biodegradation and reactive oxygen species (ROS) scavenger genes to protect from oxygen toxicity. The inner biofilm community contained obviously higher cytochrome c genes responsible for extracellular electron transfer. Additionally, network analysis indicated the aniline degraders were positively associated with electroactive bacteria and could be the potential hosts for genes encoding for dioxygenase and cytochrome, respectively. This study provides a feasible strategy to enhance nitrogen-containing organics ammonification and offers new insights into the microbial interaction mechanisms of micro-aeration assisted with electrogenic respiration.

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This study aimed at evaluating the microaeration as an alternative for hydrogen sulfide removal from biogas of UASB reactors treating sewage. The set-up consisted of two pilot-scale UASB reactors, including a conventional anaerobic and a modified UASB reactor, operated under microaerated conditions. Air was supplied in the digestion zone, at 1 and 3 m from the bottom of the reactor, and three different air flows were investigated: 10, 20, and 30 mL.min-1, corresponding to 0.003, 0.005 and 0.005 LO2/Linfluent, respectively. The main results showed that the microaeration provided a substantial decrease in hydrogen sulfide concentrations when compared to the concentrations observed in the biogas of the anaerobic UASB reactor. Hydrogen sulfide concentrations remained below 70 ppmv throughout the experimental period, corresponding to an average removal efficiency of 98%. Although a decrease in methane concentrations in biogas was observed, the feasibility of energy use would not be affected. The effect of microaeration on the overall performance of the reactor was evaluated, however, no significant differences were observed. The feasibility of limiting aeration conditions in the reactor digestion zone as an efficient alternative for hydrogen sulfide removal from biogas was demonstrated.

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In this study, different micro-aeration (MA) strategies for anaerobic digestion (AD) of poultry litter (PL) and wheat straw (WS) were examined. MA at different stages (pretreatment, middle, pretreatment plus middle, and daily) in batch AD of WS showed that daily MA had the highest increase (16.5 %) of the cumulative methane yield (CMY) compared to the control. Batch co-digestion (Co-AD) of WS and PL with daily MA obtained a furtherly improved (15.1 %) CMY of 225.44 N mL CH4/g vS added. The modified Gompertz model and Cone model were good in fitting the methane yield kinetics of MA engaged AD process (R2 greater than 0.99). Daily MA shortened the lag phase of Co-AD by 3.4 %. The sequencing batch reactor for the Co-AD of WS and PL showed an increased (21.5 %) daily methane yield when 0.5-h/d MA was employed. The results provided support for the application of micro-aeration in the AD of agricultural wastes.

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Micro-aeration can facilitate anaerobic digestion (AD) by regulating microbial communities and promoting the growth of facultative taxa, thereby increasing methane yield and stabilizing the AD process. Additionally, micro-aeration contributes to hydrogen sulfide stripping by oxidization to produce molecular sulfur or sulfuric acid. Although micro-aeration can positively affect AD, it must be strictly regulated to maintain an overall anaerobic environment that permits anaerobic microorganisms to thrive. Even so, obligate anaerobes, especially methanogens, could suffer from oxidative stress during micro-aeration. This review describes the applications of micro-aeration in AD and examines the cutting-edge advances in how methanogens survive under oxygen stress. Moreover, barriers and corresponding solutions are proposed to move micro-aeration technology closer to application at scale.

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The landfill is still the primary waste treatment method in developing countries. Due to the long stability time and long-term occupation of a large amount of land, the landfill poses a significant threat to the ecological environment and affects the process of urbanization. This study conducted a landfill simulation reactor (LSR) experiment to achieve rapid landfill stabilization through micro-aeration and leachate recirculation. More than 60 % of the degradable organic carbon in the enhanced process (LSR-IV contains 24 % of the retained carbon) can be relatively quickly converted to a gaseous state, which is nearly half higher than the degradation efficiency of the traditional process (LSR-I contains 59.3 % of the retained carbon). A comprehensive environmental assessment is developed for the enhanced process, and better environmental benefits are obtained from the whole landfill process. Compared with conventional treatment process, the enhanced process is applied to the actual landfill to analyze the economic cost. In terms of the total cost, the enhanced process cost (60.1 CNY) is about 44 % lower than the conventional landfill process cost (107.6 CNY). The enhanced process saves nearly half of the time cost and reduces the cost of land acquisition. This study can provide a reference for governmental and municipal administrations to carry out the technological transformation of traditional landfills from the aspects of technology, economy and environment.

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In high-load anaerobic digestion such as in kitchen waste, side-stream micro-aeration (SMA) shows excellent operational performance to direct micro-aeration (DMA). It immediately restores the acidification to stability. Methanogenic performance remained stable when organic load ratios (OLR) was further increased to 5.5 g VS/L. Enhanced enzyme activity, microbial aggregation, and proliferation of bacteria and archaea were observed in SMA. The results indicates that SMA enriched Methanosaeta (relative abundance exceeded 93%) and induced the change of the main methanogenic pathway to acetoclastic methanogenesis. Mechanisms was further explored by using metagenomic analysis, and the results show SMA avoids mass formation of ROS (reactive oxygen species) by cycling the aerated slurry, and retains benefits of trace O2 on material and energic metabolism, which poses great application potentials and deserves further investigation.

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Micro-aeration hydrolysis acidification (HA) is an effective method to enhance the removal of toxic and refractory organic matter, but the difficulty in stable dosing control of trace oxygen limits its wide application. Membrane-based bubbleless aeration has been proved as an ideal aeration method because of its higher oxygen transfer rate, more uniform mass transfer, and lower cost than HA. However, the available information on its application in HA is limited. In this study, membrane-based bubbleless micro-aeration coupled with hydrolysis acidification (MBL-MHA) was exploited to investigate the performance of 2,4-dinitrophenol (2,4-DNP) degradation via comparing it with bubble micro-aeration HA (MHA) and anaerobic HA. The results indicated that the performances in MBL-MHA and MHA were higher than those in HA during the experiment. 2,4-DNP degradation rates under redox microenvironments caused by counter-diffusion in MBL-MHA (84.43∼97.28%) were higher than those caused by co-diffusion in MHA (82.41∼94.71%) under micro-aeration of 0.5-5.0 mL air/min. The 2,4-DNP degradation pathways in MBL-MHA were nitroreduction, deamination, aromatic ring cleavage, and fermentation, while those in MHA were hydroxylation, aromatic ring cleavage, and fermentation. Reduction/oxidation-related, interspecific electron transfer-related species, and fermentative species in MBL-MHA were more abundant than that in MHA. Ultimately, more reducing/oxidizing forces formed by more redox proteins/enzymes from these rich species could enhance 2,4-DNP degradation in MBL-MHA.

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