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Pharmaceutical uptake involves processes that vary across aquatic systems and biota. However, single studies examining multiple environmental compartments, microhabitats, biota, and exposure pathways in mesoconsumer fish are sparse. We investigated the pharmaceutical burden in bonefish (Albula vulpes), pathways of exposure, and estimated exposure to a human daily dose. To evaluate exposure pathways, the number and composition of pharmaceuticals across compartments and the bioconcentration in prey and bonefish were assessed. To evaluate bioaccumulation, we proposed the use of a field-derived bioaccumulation factor (fBAF), due to variability inherent to natural systems. Exposure to a human daily dose was based on bonefish daily energetic requirements and consumption rates using pharmaceutical concentrations in prey. Pharmaceutical number and concentration were highest in prey, followed by bonefish, water and sediment. Fifteen pharmaceuticals were detected in common among bonefish, prey, and water; all of which bioconcentrated in prey and bonefish, and four bioaccumulated in bonefish. The composition of detected pharmaceuticals was compartment specific, and prey were most similar to bonefish. Bonefish were exposed to a maximum of 1.2 % of a human daily dose via prey consumption. Results highlight the need for multicompartment assessments of exposure and consideration of prey along with water as a pathway of exposure.

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To date, the presence of pharmaceuticals has been extensively documented across a wide range of aquatic systems and biota. Further, substantial progress has been made in transitioning from laboratory assessments of pharmaceutical fate and effects in fish to in situ assessments of exposure and effects; however, certain research areas remain understudied. Among these is investigation of differential accumulation across multiple internal tissues in wild marine fish beyond the species commonly sampled in laboratory and freshwater field settings. This study examined the presence of pharmaceuticals across four tissues (plasma, muscle, brain, and liver) in a wild marine fish, bonefish (Albula vulpes), throughout coastal South Florida, USA. Differential accumulation across tissues was assessed for the number and concentration, identity, and composition of accumulated pharmaceuticals by sampling 25 bonefish and analyzing them for 91 pharmaceuticals. The concentration of pharmaceuticals was highest in plasma > liver > brain > muscle, while the number of pharmaceuticals was highest in liver > brain > plasma > muscle. The identity of detected pharmaceuticals was tissue specific, and there was an inverse relationship between the number of detections for each pharmaceutical and its log Kow. The composition of pharmaceuticals was tissue specific for both pharmaceutical presence/absence and concentration. Across all tissues, the greatest similarity was between brain and liver, which were more similar to plasma than to muscle, and muscle was the most distinct tissue. For tissue compositional variability, muscle was the most diverse in accumulated pharmaceuticals, while plasma, brain, and liver were similarly variable. With the highest concentrations in plasma and highest number in liver, and documented variability in accumulated pharmaceuticals across tissues, our results highlight the importance of tissue selection when surveying exposure in wild fish, suggesting that multi-tissue analysis would allow for a more comprehensive assessment of exposure diversity and risk of adverse effects.

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Iron is a key micronutrient essential for various essential biological processes. As a consequence, alteration in iron concentration in seawater can deeply influence marine biodiversity. In polar marine environments, where environmental conditions are characterized by low temperatures, the role of iron becomes particularly significant. While iron limitation can negatively influence primary production and nutrient cycling, excessive iron concentrations can lead to harmful algal blooms and oxygen depletion. Furthermore, the growth of certain phytoplankton species can be increased in high-iron-content environments, resulting in altered balance in the marine food web and reduced biodiversity. Although many chemical/physical methods are established for inorganic iron quantification, the determination of the bio-available iron in seawater samples is more suitably carried out using marine microorganisms as biosensors. Despite existing challenges, whole-cell biosensors offer other advantages, such as real-time detection, cost-effectiveness, and ease of manipulation, making them promising tools for monitoring environmental iron levels in polar marine ecosystems. In this review, we discuss fundamental biosensor designs and assemblies, arranging host features, transcription factors, reporter proteins, and detection methods. The progress in the genetic manipulation of iron-responsive regulatory and reporter modules is also addressed to the optimization of the biosensor performance, focusing on the improvement of sensitivity and specificity.

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Marine ecosystems are facing a dramatic loss of biodiversity worldwide, together with a widespread collapse of habitats and their functionality. In this context, Marine Citizen Science (MCS) can be a powerful tool to monitor these changes over time. The flowering of very well-structured international projects is strengthening the scientific credibility of MCS data, especially when data are collected after specifically designed training programs and shared in public user-friendly repositories. Here we present a new perspective on the use of MCS in the Mediterranean area, along with the main benefits for the stakeholders (i.e., diving centers, trainers, and policymakers) and the users (i.e., divers), resumed in three pillars: Pillar I - MCS as a tool for the site valorization; Pillar II - MCS as a new career opportunity for graduated students; Pillar III - MCS as a business opportunity for diving centers. In the frame of the Quintuple Helix Approach, for which there is a strong need of a socioecological transition of the society and economy, we show how MCS can be a win-win-win solution for all the actors involved, providing the vision for new and highly qualified job and business opportunities for the diving sector.

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Understanding the diversity and functions of hydrocarbon-degrading microorganisms in marine environments is crucial for both advancing knowledge of biogeochemical processes and improving bioremediation methods. In this study, we leveraged nearly 20,000 metagenome-assembled genomes (MAGs), recovered from a wide array of marine samples across the global oceans, to map the diversity of aerobic hydrocarbon-degrading microorganisms. A broad bacterial diversity was uncovered, with a notable preference for degrading aliphatic hydrocarbons over aromatic ones, primarily within Proteobacteria and Actinobacteriota. Three types of broad-spectrum hydrocarbon-degrading bacteria were identified for their ability to degrade various hydrocarbons and possession of multiple copies of hydrocarbon biodegradation genes. These bacteria demonstrate extensive metabolic versatility, aiding their survival and adaptability in diverse environmental conditions. Evidence of gene duplication and horizontal gene transfer in these microbes suggested a potential enhancement in the diversity of hydrocarbon-degrading bacteria. Positive correlations were observed between the abundances of hydrocarbon-degrading genes and environmental parameters such as temperature (-5 to 35 °C) and salinity (20 to 42 PSU). Overall, our findings offer valuable insights into marine hydrocarbon-degrading microorganisms and suggest considerations for selecting microbial strains for oil pollution remediation.

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Pharmaceutical compounds are micropollutants of emerging concern, as well as other classes of chemicals such as UV filters and artificial sweeteners. They enter marine environments via wastewater treatment plants, aquaculture runoff, hospital effluents, and shipping activities. While many studies have investigated the presence and distribution of these pollutants in numerous coastal areas, our study is the first to focus on their occurrence, spatial distribution, and vertical distribution in the sea surface microlayer (SML) and the near-surface layer of marine environments. We analyzed 62 pharmaceutical compounds, one UV filter, and six artificial sweeteners from the SML to the corresponding underlying water (0 cm, 20 cm, 50 cm, 100 cm, and 150 cm) at four stations in the southern North Sea. One station is the enclosed Jade Bay, one is the Weser estuary at Bremerhaven, and the other two stations (NS_7 and NS_8) are in the open German Bight. Jade Bay receives pollutants from surrounding wastewater treatment plants, while the Weser estuary receives pollutants from cities like Bremerhaven, which has dense populations and industrial activities. Concentrations of pharmaceutical compounds were higher in the upper water layers (from the SML to 20 cm). Eleven pharmaceutical compounds (caffeine, carbamazepine, gemfibrozil, ibuprofen, metoprolol, salicylic acid, clarithromycin, novobiocin, clindamycin, trimethoprim, and tylosin) were detected in >95 % of our samples. One UV filter (benzophenone-4) was found in 83 % and three artificial sweeteners (acesulfame, saccharin, and sucralose) in 100 % of all our samples. All artificial sweeteners posed high risks to the freshwater invertebrate Daphnia magna. Understanding the spatial and vertical distribution of pharmaceuticals and other micropollutants in marine environments may be essential in assessing their dispersal and detection in other aquatic environments.

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The pollution of microplastics (MPs) to the marine environment has become a widespread focus of attention. To assess MP-induced ecotoxicity on marine ecosystems, periphytic protozoan communities were used as test organisms and exposed to five concentrations of MPs: 0, 1, 5, 25, and 125 mg l-1. Protozoan samples were collected using microscope slides from coastal waters of the Yellow Sea, northern China. A total of 13 protozoan species were identified and represented different tolerance to MP-induced ecotoxicity. Inhibition effects of MPs on the test protozoan communities were clearly shown in terms of both the species richness and individual abundance and followed linear relationships to MP concentrations. The community patterns were driven by MPs and significantly shifted at concentrations over 5 mg l-1. Our findings demonstrated that MPs may induce the community-level ecotoxic response of periphytic protozoan fauna and followed significant community dynamics. Thus, it is suggested that periphytic protozoan fauna may be used as useful community-based test model organisms for evaluating MP-induced ecotoxicity in marine environments.

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The global ocean has been receiving massive amounts of plastic wastes. Marine biodegradation, influenced by global climate, naturally breaks down these wastes. In this study, we systematically compared the biodegradation performance of petroleum- and bio-based plastic films, i.e., low-density polyethylene (LDPE), polylactic acid (PLA), and polyhydroxyalkanoates (PHAs) under three ambient temperatures (4, 15, and 22 °C). We deployed the our previously isolated cold-tolerant plastic-degrading Alcanivorax to simulate the accelerated marine biodegradation process and evaluated the alteration of bacterial growth, plastic films, and released degradation products. Notably, we found that marine biodegradation of PHA films enriched more bacterial amounts, induced more conspicuous morphological damage, and released more microplastics (MPs) and dissolved organic carbon (DOC) under all temperatures compared to LDPE and PLA. Particularly, MPs were released from film edges and cracks with a mean size of 2.8 µm under all temperatures. In addition, the degradation products released by biodegradation of PHA under 22 °C induced the highest acute toxicity to Vibrio fischeri. Our results highlighted that: (1) marine biodegradation of plastics would release millions of MPs per cm2 exposed surface area even in cold environments within 60 days; (2) different marine biodegradation scenarios of these plastics may raise disparate impacts and mitigation-related studies.

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Most research on pharmaceutical presence in the environment to date has focused on smaller scale assessments of freshwater and riverine systems, relying mainly on assays of water samples, while studies in marine ecosystems and of exposed biota are sparse. This study investigated the pharmaceutical burden in bonefish (Albula vulpes), an important recreational and artisanal fishery, to quantify pharmaceutical exposure throughout the Caribbean Basin. We sampled 74 bonefish from five regions, and analyzed them for 102 pharmaceuticals. We assessed the influence of sampling region on the number of pharmaceuticals, pharmaceutical assemblage, and risk of pharmacological effects. To evaluate the risk of pharmacological effects at the scale of the individual, we proposed a metric based on the human therapeutic plasma concentration (HTPC), comparing measured concentrations to a threshold of 1/3 the HTPC for each pharmaceutical. Every bonefish had at least one pharmaceutical, with an average of 4.9 and a maximum of 16 pharmaceuticals in one individual. At least one pharmaceutical was detected in exceedance of the 1/3 HTPC threshold in 39% of bonefish, with an average of 0.6 and a maximum of 11 pharmaceuticals exceeding in a Key West individual. The number of pharmaceuticals (49 detected in total) differed across regions, but the risk of pharmacological effects did not (23 pharmaceuticals exceeded the 1/3 HTPC threshold). The most common pharmaceuticals were venlafaxine (43 bonefish), atenolol (36), naloxone (27), codeine (27), and trimethoprim (24). Findings suggest that pharmaceutical detections and concentration may be independent, emphasizing the need to monitor risk to biota regardless of exposure diversity, and to focus on risk quantified at the individual level. This study supports the widespread presence of pharmaceuticals in marine systems and shows the utility of applying the HTPC to assess the potential for pharmacological effects, and thus quantify impact of exposure at large spatial scales.

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To investigate effects of salinity variability on colonization dynamics of periphytic protozoan fauna, a 21-day study was conducted in temperature-controlled circulation systems (TCCSs). Periphytic protozoan communities were incubated using glass slides as artificial substrata in five TCCS aquaria with a large-scale salinity gradient of 9, 19, 29 (control), 39, and 49 PSU, respectively. The colonization dynamics were observed on days 3, 5, 7, 10, 14, and 21. The colonization dynamics were well fitted to the MacArthur-Wilson and logistic model equations in colonization and growth curves in all five treatments, respectively. However, the maximum species richness and abundance were reduced, and the colonization patterns were significantly shifted in four treatments with salinity changed by 20 PSU compared to the control (29 PSU). Thus, it is suggested that the large-scale salinity variability may reduce the species richness significantly and affect colonization dynamics of periphytic protozoan fauna in marine environments.

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IMPORTANCE: PP biodegradation has not been clearly shown (it has been uncertain whether the PP structure is actually biodegraded or not). This is the first report on the obvious biodegradation of PP. At the same time, this study shows that Alcanivorax bacteria could be major degraders of PP in mesopelagic environments. Moreover, PP biodegradation has been investigated by using solid PP as the sole carbon source. However, this study shows that PP would not be used as a sole carbon and energy source. Our data thus provide very important and key knowledge for PP bioremediation.

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Climate change is affecting the distribution of marine organisms worldwide, including venomous marine gastropods that offer risks to human health, but also potential pharmacological resources, such as Conus sp. Species Distribution Models (SDMs) are valuable tools for predicting species distribution under climate change. The objective of our study was to evaluate the potential distribution of Conus geographus and C. textile in the Indo-Pacific region under different climate change scenarios for 2050 and 2090. We constructed SDMs with MaxEnt for each species, using bioclimatic variables from Bio-ORACLE and NOAA, and occurrence data from GBIF. We projected the best-fit model for the present and different future climate change scenarios (SSP1-2.6, SSP2-4.5, SSP3-7.0, SSP5-8.5). We obtained high accuracy SDMs for C. geographus and C. textile, with Temperature and Primary Productivity as the main explanatory variables. Our future projections reveal that both species may react differently to climate change. Southeast Asia and Micronesia will continue to provide a climatically appropriate environment for both species; however, they may become more suitable for C. geographus and less suitable for C. textile. This may lead to a higher risk of human envenomation by C. geographus, but a lower risk by C. textile. A decreased suitability for C. textile may also lead to the loss of potential pharmacological resources among its range. Our study emphasizes how SDMs can be used to assess the future distribution of species with human health implications, which can aid in the monitoring of venomous marine species.

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This paper is focused on the use of radio frequency identification (RFID) technology operating at 125 kHz in a communication layer for a network of mobile and static nodes in marine environments, with a specific focus on the Underwater Internet of Things (UIoT). The analysis is divided into two main sections: characterizing the penetration depth at different frequencies and evaluating the probabilities of data reception between antennas of static nodes and a terrestrial antenna considering the line of sight (LoS) between antennas. The results indicate that the use of RFID technology at 125 kHz allows for data reception with a penetration depth of 0.6116 dB/m, demonstrating its suitability for data communication in marine environments. In the second part of the analysis, we examine the probabilities of data reception between static-node antennas at different heights and a terrestrial antenna at a specific height. Wave samples recorded in Playa Sisal, Yucatan, Mexico, are used for this analysis. The findings show a maximum reception probability of 94.5% between static nodes with an antenna at a height of 0 m and a 100% data reception probability between a static node and the terrestrial antenna when the static-node antennas are optimally positioned at a height of 1 m above sea level. Overall, this paper provides valuable insights into the application of RFID technology in marine environments for the UIoT, considering the minimization of impacts on marine fauna. The results suggest that by adjusting the characteristics of the RFID system, the proposed architecture can be effectively implemented to expand the monitoring area, considering variables both underwater and on the surface of the marine environment.

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As is the case for many others in the world, Mexican seas face complex pollution challenges; two of the contaminants that require special attention for their prevalence, possible chemical interactions, and relation to the country's economy are leaked petroleum and microplastics (MP). This research assessed the sorption of total petroleum hydrocarbons (TPH) as fuel oil on microplastics in laboratory and field scenarios. Preliminary tests allowed the development and validation of a methodology to measure the sorbed fuel oil by Soxhlet extraction, with a 99.65% recovery rate. The amount of TPH sorbed in the lab followed the order LDPE > PS > PP > PVC > PET > HDPE, with the highest concentration found on LDPE. The sorption of fuel oil on microplastics is correlated to the surface area of the plastic particles and could also be related to the crystallinity of plastics. Sorption, for all plastics, was consistent with a second-order kinetic model. The analysis of field samples collected on beaches of the Gulf of Mexico varied from 1660 to 35,258 mg/kg MP. It must be noticed that, unlike others, this research quantified a family of contaminants, which could explain the high concentrations observed on microplastics.

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Measured salt compositions in dust collected over roughly the last decade from surfaces of in-service stainless-steel alloys at four locations around the United States are presented, along with the predicted brine compositions that would result from deliquescence of these salts. The salt compositions vary greatly from ASTM seawater and from laboratory salts (i.e., NaCl or MgCl2) commonly used on corrosion testing. The salts contained relatively high amounts of sulfates and nitrates, evolved to basic pH values, and exhibited deliquescence relative humidity values (RH) higher than seawater. Additionally, inert dust in components were quantified and considerations for laboratory testing are presented. The observed dust compositions are discussed in terms of the potential corrosion behavior and are compared to commonly used accelerated testing protocols. Finally, ambient weather conditions and their influence on diurnal fluctuations in temperature (T) and RH on heated metal surfaces are evaluated and a relevant diurnal cycle for laboratory testing a heated surface has been developed. Suggestions for future accelerated tests are proposed that include exploration of the effects of inert dust particles on atmospheric corrosion, chemistry considerations, and realistic diurnal fluctuations in T and RH. Understanding mechanisms in both realistic and accelerated environments will allow development of a corrosion factor (i.e., scaling factor) for the extrapolation of laboratory-scale test results to real world applications.

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Microbially influenced corrosion (MIC) of metals is an important industrial problem, causing 300-500 billion dollars of economic loss worldwide each year. It is very challenging to prevent or control the MIC in the marine environment. Eco-friendly coatings embedded with corrosion inhibitors developed from natural products may be a successful approach for MIC prevention or control. As a natural renewable resource, cephalopod chitosan has a number of unique biological properties, such as antibacterial, antifungal and non-toxicity effects, which attract scientific and industrial interests for potential applications. Chitosan is a positively charged molecule, and the negatively charged bacterial cell wall is the target of its antimicrobial action. Chitosan binds to the bacterial cell wall and disrupts the normal functions of the membrane by, for example, facilitating the leakage of intracellular components and impeding the transport of nutrients into the cells. Interestingly, chitosan is an excellent film-forming polymer. Chitosan may be applied as an antimicrobial coating substance for the prevention or control of MIC. Furthermore, the antimicrobial chitosan coating can serve as a basal matrix, in which other antimicrobial or anticorrosive substances like chitosan nanoparticles, chitosan silver nanoparticles, quorum sensing inhibitors (QSI) or the combination of these compounds, can be embedded to achieve synergistic anticorrosive effects. A combination of field and laboratory experiments will be conducted to test this hypothesis for preventing or controlling MIC in the marine environment. Thus, the proposed review will identify new eco-friendly MIC inhibitors and will assay their potential in future applications in the anti-corrosion industry.

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: Despite its small area, Araçá Bay (AB) holds cultural, historical, and economic value and displays great benthic biodiversity. Thus, it is crucial to monitor its environmental health, including knowing the main groups of phytoplankton and their temporal variability. The shallow waters of Araçá Bay are continuously modified by the complex hydrography of the adjacent São Sebastião channel (SSC), challenging standard experimental designs for phytoplankton collection. Here we report changes in phytoplankton composition at intervals of five to six weeks from September 2013 to August 2014 in both Araçá Bay and SSC. Samples were collected twice daily for three consecutive days to increase taxonomic resolution. Our goal was to provide an inventory of species occurrences to aid future public policies and environmental management of the area. Analyses revealed high species richness and 166 different phytoplankton taxa. Diatoms and dinoflagellates were always numerically dominant, but taxa occurrence changed markedly. Diatoms of the genera Pseudo-nitzschia were abundant during spring and summer concurrently to signatures of South Atlantic Central Water in the SSC, while Thalassiosira occurred when waters displayed relatively lower salinity. The inventory demonstrated several potentially harmful species of microalgae and cyanobacteria, strongly suggesting investments in monitoring programs in this area that currently experience an increase in population.

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Mercury (Hg) has seven stable isotopes that can be utilized to trace the sources of Hg and evaluate the importance of transport and transformation processes in the cycling of Hg in the environment. The ocean is an integral part of the Earth and plays an important role in the global mercury cycle. However, there is a lack of a systematic review of Hg stable isotopes in marine environments. This review is divided into four sections: a) advances in Hg stable isotope analysis, b) the isotope ratios of Hg in various marine environmental matrices (seawater, sediment, and organisms), c) processes governing stable Hg isotope ratios in the ocean, and d) application of Hg stable isotopes to understand biotic uptake and migration. Mercury isotopes have provided much useful information on marine Hg cycling that cannot be given by Hg concentrations alone. This includes (i) sources of Hg in coastal or estuarine environments, (ii) transformation pathways and mechanisms of different forms of Hg in marine environments, (iii) trophic levels and feeding guilds of marine fish, and (iv) migration/habitat changes of marine fish. With the improvement of methods for seawater Hg isotope analysis (especially species-specific methods) and the measurement of Hg isotope fractionation during natural biogeochemical processes in the ocean, Hg stable isotopes will advance our understanding of the marine Hg cycle in the future, e.g., mercury exchange at the sea-atmosphere interface and seawater-sediment interface, contributions of different water masses to Hg in the ocean, fractionation mechanisms of Hg and MeHg transformation in seawater.

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Four extremely halophilic archaeal strains (ZJ2T, BND6T, DT87T, and YPL30T) were isolated from marine environments and a salt mine in China. The 16S rRNA and rpoB' gene sequence similarities among strains ZJ2T, BND6T, DT87T, YPL30T and the current species of Natrinema were 93.2-99.3% and 89.2-95.8%, respectively. Both phylogenetic and phylogenomic analyses revealed that strains ZJ2T, BND6T, DT87T, and YPL30T cluster with the Natrinema members. The overall genome-related indexes (ANI, isDDH, and AAI) among these four strains and the current species of genus Natrinema were 70-88%, 22-43% and 75-89%, respectively, clearly below the threshold values for species boundary. Strains ZJ2T, BND6T, DT87T, and YPL30T could be distinguished from the related species according to differential phenotypic characteristics. The major polar lipids of the four strains were phosphatidic acid (PA), phosphatidylglycerol (PG), phosphatidylglycerol phosphate methyl ester (PGP-Me), sulfated mannosyl glucosyl diether (S-DGD-1), and disulfated mannosyl glucosyl diether (S2-DGD). The phenotypic, chemotaxonomic, phylogenetic and phylogenomic features indicated that strains ZJ2T (= CGMCC 1.18786 T = JCM 34918 T), BND6T (= CGMCC 1.18777 T = JCM 34909 T), DT87T (= CGMCC 1.18921 T = JCM 35420 T), and YPL30T (= CGMCC 1.15337 T = JCM 31113 T) represent four novel species of the genus Natrinema, for which the names, Natrinema caseinilyticum sp. nov., Natrinema gelatinilyticum sp. nov., Natrinema marinum sp. nov., and Natrinema zhouii sp. nov., are proposed.

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Species from shallow marine environments are particularly vulnerable to extreme weather events (heatwaves and extreme rainfall) that can promote abrupt environmental shifts, namely in temperature and salinity (respectively). To assess how these shifts impact species' cellular stress responses (CSR), ditch shrimps Palaemon varians were exposed to a chronic (28 days) thermohaline stress experiment. Three levels of temperature (20, 23 and 26 °C) and two levels of salinity (20 and 40) were tested in a full factorial experiment, and shrimps sampled at the 7th, 14th, 21st and 28th day of exposure. Survival, wet weight (as proxy for growth), and cellular stress biomarkers associated with oxidative stress (LPO - Lipid Peroxidation, GST - Glutathione-S-Transferase, SOD - Superoxide Dismutase, TAC - Total Antioxidant Capacity and CAT - Catalase) and protein denaturation (UBI - Ubiquitin and HSP-70 - Heat Shock Protein 70 kDa) were analysed in shrimps' muscle at each sampling day. Temperature and time of exposure significantly affected biomarker levels, with shrimps exposed to 20 and 26 °C revealing more pronounced differences. No interactions were detected between temperature and salinity, suggesting that these factors display additive effects on shrimps' CSR. Antioxidant agents (CAT and TAC) increased under elevated temperature, while protein denaturation markers (UBI and HSP-70) were mostly affected by time of exposure, decreasing at 28 days. Total protein reserves increased throughout time and no effects on wet weight were observed. A negative correlation between wet weight and HSP-70 was detected, suggesting that HSP-70 levels are dependent on organism size. Peak survival (~73 %) was found under 20 °C and salinity 40 and lower survival (~30-40 %) was associated with higher temperatures (23 and 26 °C) and lower salinity (20). We conclude that P. varians displays some level of acclimation capacity but differences in survival may indicate effects on osmoregulation processes and the need for longer timeframes to fully acclimate to heat and hyposaline stress.

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