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Mosquito surveillance is critical for actively tracking the location and monitoring population levels and the threat of mosquito-borne disease. Although light-emitting diodes (LEDs) light traps have grown in popularity, there is still a limited understanding of the application of light wavelengths for trapping nocturnally active wild mosquitoes in forest ecotypes. This study evaluated the performance of different UV wavelengths in trapping mosquito populations in a forested mountainous area in Nakhon Ratchasima province, Thailand. Traps with different UV wavelengths were deployed in 6 locations, following a 6 × 6 Latin square replicated 6 times over a total of 36 nights. Light traps were operated between 18:00 and 06:00 h from October 2022 to August 2023. Mosquitoes were separately collected from individual traps every 4 h at 22.00, 2.00, and 6.00 h. Mosquitoes were killed by placing in a freezer (- 20 °C) for at least 30 min and then were morphologically identified using illustrated keys for adult females. Traps fitted with the LED 365 wavelength light source were the most effective in capturing 790 (23.66%) of the total mosquitoes collected, followed by the UV fluorescent 632 (18.93%), with the other 4 LED wavelengths collecting between 16.89% (LED 385) and 12.64% (LED 375) of the mosquitoes. Culex was the most common genus, representing 56.00% of total mosquito abundance. LED 365 and LED 385 were comparable to the UV fluorescent traps (the standard reference). Optimal trapping times were during 18:00-22:00 h. Compared to the other wavelengths, LED 365 was significantly more effective at capturing Coquillettidia and Culex mosquitoes than the UV-based traps.

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BACKGROUND: Analyses of the temporal distribution of mosquitoes are presented in statistical charts, but it is difficult to prove in statistics whether differences in peak periods exist among different years or habitats. This study aimed to investigate the application of circular statistics in determining the peak period and a comparison of differences. METHODS: Surveillance of adult mosquitoes was conducted twice a month by light traps in five different habitats from March to November for 3 years (2021-2023) in Qingdao, Shandong Province, China. The Kruskal-Wallis test was performed to determine the differences in mosquito density among different years and habitats. Circular statistics and line charts were employed to determine the peak period and a comparison of differences. RESULTS: Among a total of 14,834 adult mosquitoes comprising five mosquito species from four genera, Culex pipiens pallens was dominant and accounted for 89.6% of the specimens identified. Aedes albopictus, Armigeres subalbatus, and Anopheles sinensis made up 5.7%, 4.2%, and 0.5%, respectively. Culex tritaeniorhynchus accounted for less than 0.1%. The mean mosquito density (females/trap night) for the trapping period was 10.3 in 2021, 5.6 in 2022, and 3.6 in 2023. Among five habitats, the highest mosquito density was 8.9 in livestock sheds, followed by 6.8 in parks, 5.9 in rural dwellings, 5.5 in urban dwellings, and 5.4 in hospitals. No statistically significant differences were found among different years (H = 1.96, d.f. 2, P = 0.376) and habitats (H = 0.45, d.f. 4, P = 0.978). Overall, the peak period of mosquito activity fell in the months from June to September. The peak period among 3 years differed significantly (F(2,7022) = 119.17, P < 0.01), but there were no statistically significant differences in peak period among different habitats (F(4,7020) = -159.09, P > 0.05). CONCLUSION: Circular statistics could be effectively combined with statistical charts to elucidate the peak period of mosquitoes and determine the differences in statistics among different years and habitats. These findings will provide valuable information for mosquito control and public health management.

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Fluctuations in reef fish settlement and recruitment significantly impact local population dynamics and adult population size. We use light traps to evaluate reef fish settlement in inshore and offshore reefs in the Abrolhos Bank, in addition to the spatio-temporal distribution patterns, and environmental variables' influence. Data were collected from inshore and offshore reefs, and statistical analyses, including GLMs, PERMANOVA and nMDS, aimed to understand spatial-temporal species distribution patterns and the relationship between environmental variables. Results indicate greater species abundance and diversity offshore, influenced by winds, tides, and distance from shore, explaining 50% of larval patterns and highlighting the importance of spatial-temporal variations in early-life reef fish assemblages. However, several factors, including habitat type, quality, complexity, seascape configuration, and MPA location, may influence these patterns. Additionally, we provide evidence that commercially important species like Lutjanids use Abrolhos reef areas as nursery grounds.

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BACKGROUND: Vector surveillance is among the World Health Organization global vector control response (2017-2030) pillars. Human landing catches are a gold standard but difficult to implement and potentially expose collectors to malaria infection. Other methods like light traps, pyrethrum spray catches and aspiration are less expensive and less risky to collectors. METHODS: Three mosquito sampling methods (UV light traps, CDC light traps and Prokopack aspiration) were evaluated against human landing catches (HLC) in two villages of Rarieda sub-county, Siaya County, Kenya. UV-LTs, CDC-LTs and HLCs were conducted hourly between 17:00 and 07:00. Aspiration was done indoors and outdoors between 07:00 and 11:00 a.m. Analyses of mosquito densities, species abundance and sporozoite infectivity were performed across all sampling methods. Species identification PCR and ELISAs were done for Anopheles gambiae and Anopheles funestus complexes and data analysis was done in R. RESULTS: Anopheles mosquitoes sampled from 608 trapping efforts were 5,370 constituting 70.3% Anopheles funestus sensu lato (s.l.), 19.7% Anopheles coustani and 7.2% An. gambiae s.l. 93.8% of An. funestus s.l. were An. funestus sensu stricto (s.s.) and 97.8% of An. gambiae s.l. were Anopheles arabiensis. Only An. funestus were sporozoite positive with 3.1% infection prevalence. Indoors, aspiration captured higher An. funestus (mean = 6.74; RR = 8.83, P < 0.001) then UV-LT (mean = 3.70; RR = 3.97, P < 0.001) and CDC-LT (mean = 1.74; RR = 1.89, P = 0.03) compared to HLC. UV-LT and CDC-LT indoors captured averagely 0.18 An. arabiensis RR = 5.75, P = 0.028 and RR = 5.87, P = 0.028 respectively. Outdoors, UV-LT collected significantly higher Anopheles mosquitoes compared to HLC (An. funestus: RR = 5.18, P < 0.001; An. arabiensis: RR = 15.64, P = 0.009; An. coustani: RR = 11.65, P < 0.001). Anopheles funestus hourly biting indoors in UV-LT and CDC-LT indicated different peaks compared to HLC. CONCLUSIONS: Anopheles funestus remains the predominant mosquito species. More mosquitoes were collected using aspiration, CDC-LTs and UV-LTs indoors and UV-LTs and CD-LTs outdoors compared to HLCs. UV-LTs collected more mosquitoes than CDC-LTs. The varied trends observed at different times of the night suggest that these methods collect mosquitoes with diverse activities and care must be taken when interpreting the results.

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Environmentally friendly technologies for the prevention and control of crop diseases and insect pests are important to reduce the use of chemical pesticides, improve the quality of agricultural products, protect the environment, and promote sustainable development of crop production. On the basis of Internet of Things (IoT) technology, we developed a prevention and control system for crop diseases and insect pests with two main components: a plant protection device (the hardware) and an information management system (the software). To be suitable for both facility- and field-based production scenarios, we incorporated two types of plant protection devices, utilizing ozone sterilization and light-trap technologies. The devices were equipped with various sensors to realize real-time collection and monitoring of data on the crop production environment. The information management system has an IoT-based architecture and includes a mobile device app to enable remote control of the plant protection devices for intelligent management of plant protection data. The system can achieve efficient management of large-scale equipment applications and multi-device collaborative work to prevent and control pests and diseases. The developed system has operated successfully for several years in China and has been applied to cucumber, tomato, rice, and other crops. We demonstrate the effectiveness and practicality of the system in a greenhouse facility and in the field.

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Light traps are a useful method for monitoring and controlling the important migratory pest, the fall armyworm, Spodoptera frugiperda. Studies have shown that S. frugiperda is sensitive to blue, green, or ultraviolet (UV) light, but the conclusions are inconsistent. Furthermore, conventional black light traps are less effective for trapping S. frugiperda. To improve the trapping efficiency of this pest, it is crucial to determine the specific wavelength to which S. frugiperda is sensitive and measure its flight capability under that wavelength. This study investigated the effects of light wavelength on the phototaxis and flight performance of S. frugiperda. The results showed that blue light was the most sensitive wavelength among the three different LED lights and was unaffected by gender. The flight capability of S. frugiperda varied significantly in different light conditions, especially for flight speed. The fastest flight speed was observed in blue light, whereas the slowest was observed in UV light compared to dark conditions. During a 12 h flight period, speed declined more rapidly in blue light and more slowly in UV, whereas speed remained stable in dark conditions. Meanwhile, the proportion of fast-flying individuals was highest under blue light, which was significantly higher than under UV light. Therefore, the use of light traps equipped with blue LED lights can improve the trapping efficiency of S. frugiperda. These results also provide insights for further research on the effects of light pollution on migratory insects.

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Device structure, light source height, and climatic factors can potentially affect the catching of target pests in light traps. In this study, the installation of an anti-escape cover in a newly designed light trap significantly increased the number of catches of tea leafhoppers, Empoasca onukii, an economically significant pest of tea gardens, and it prevented 97.95% of leafhoppers from escaping. A series of assessments were performed in the field and showed that the optimal trapping window of the light trap was between 1.5 and 2.5 h (2 ± 0.35 h) after sunset, and the starting time of the window was positively correlated with the sunset time. The number of leafhopper catches decreased sharply when the height of the light source was above the flight height range of E. onukii adults. The height of the light source was optimal between 20 and 40 cm above the tea canopy. The efficacy of the light traps for capturing leafhoppers decreased in the autumn peak period. High numbers of leafhopper catches by the newly designed light trap in the summer could reduce E. onukii population sizes in the autumn. Overall, the newly designed light trap can be used to reduce E. onukii adult populations in tea gardens.

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A novel design of a portable funnel light trap (PFLT) was presented for collecting insects in ecological studies. The trap consists of a compact plastic box equipped with a light source and power source, along with two plastic polypropylene interception vanes. The PFLT costs 18.3 USD per unit and weighs approximately 300 g. A maximum of six PFLT units can be packed in one medium-sized backpack (32 cm × 45 cm × 15 cm, 20 L), making it easier to set up multiple units in remote areas wherein biodiversity research is needed. The low cost and weight of the trap also allows for large-scale deployment. The design is customizable and can be easily manufactured to fit various research needs. To validate the PFLT's efficacy in collecting insects across different habitat types, a series of field experiments were conducted in South Korea and Laos, where 37 trials were carried out. The PFLT successfully collected 7497 insects without experiencing battery issues or damage by rain or wind. Insect compositions and abundances differed across the three sampled habitat types: forests, grasslands, and watersides. This new FLT trap is an important tool for studying and protecting insect biodiversity, particularly in areas wherein conventional light traps cannot be deployed.

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BACKGROUND: After several years without bluetongue disease, a ruminant illness caused by Culicoides-borne bluetongue virus (BTV), two new autochthonous cases were reported in 2018 in Germany. By contrast, Schmallenberg virus (SBV), another Culicoides-borne virus pathogenic to ruminants, has continuously circulated in Germany since its first emergence in 2011. The disease outbreaks have triggered numerous studies on the biology of the Culicoides vectors, but many ecological details are still obscure. METHODS: Culicoides biting midge species were collected with UV-light traps on 10 farms in Germany, with one trap inside and one trap outside barns on each of the farms. Traps were run once a week for 24 h from January to December 2019. Collected biting midges were morphologically identified, counted and statistically evaluated, with a focus on the Obsoletus Group and the Pulicaris Complex of the ceratopogonid genus Culicoides, which are believed to contain the major virus vectors. Temperature and relative humidity recorded at each trap were linked to the quantity of caught Culicoides. Correlations between relative Culicoides abundance and presence of livestock or type of husbandry were also investigated. RESULTS: A total of 38,886 Culicoides biting midges were trapped, with most of them belonging to the Obsoletus Group (51.0%) and the Pulicaris Complex (38.8%). The majority of captured specimens were collected in traps inside the barns. Obsoletus Group individuals were caught from late January to the last week of December while Pulicaris Complex individuals were captured from the end of March to early December. The lowest average temperatures at which members of the two groups were collected were 10.7 °C and 12.8 °C, respectively. While temperature had a statistically significant effect on the activity of both the Obsoletus Group and the Pulicaris Complex, relative humidity only significantly affected the activity of the latter. The presence of livestock significantly influenced the number of captured Obsoletus Group, but not of Pulicaris Complex specimens. Inside the barns, no statistical difference was found between numbers of caught Obsoletus Group and Pulicaris Complex specimens in livestock holdings with deep litter and manure scraper or slatted floor husbandry systems. CONCLUSIONS: The almost year-round presence of Obsoletus Group biting midges and the demonstrated high relative abundance of other potential Culicoides vector species inside barns suggest a high risk of indoor virus transmission to ruminants should BTV or SBV circulate locally. Appropriate structural, organisational and vector control measures to reduce biting midge exposure should be implemented.

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BACKGROUND: Joint efforts by government and non-government organizations have helped to reduce malaria in Bangladesh and set the country on a clear path to eventual malaria elimination. However, achieving that goal would be challenging without a comprehensive understanding of vector bionomics. METHODS: Targeted capturing of Anopheles mosquitoes over a rainy season, utilizing specific sampling methods, including human landing catches (HLCs), CDC-light traps (CDC-LTs), and pyrethrum spray catches (PSCs) were aimed to characterize entomological drivers of transmission in four sites of Bandarban, Bangladesh. RESULTS: Molecular characterization of a subset of 4637 mosquitoes has demonstrated the presence of at least 17 species whose capture rates were representative of the rainy season. Species compositions and bionomic traits did not vary between sites with Anopheles maculatus having the highest landing rate by HLCs and Anopheles vagus having the highest capture rate with CDC-LTs. Interestingly, Anopheles species compositions and capture rates varied significantly (p < 0.05) for An. vagus, between HLCs and its often-used proxy-CDC-LTs- suggesting impacts on downstream analysis. CDC-LTs capture rates demonstrated differing compositions with indoor and outdoor biting rates. For example, Anopheles nigerrimus and Anopheles nivipes were more endophagic by HLCs and more exophagic by CDC-LTs. The use of a cow-baited CDC-LT also demonstrated significantly different results when compared to a human-baited CDC-LT considering the high degree of anthropophily in these species. The exception to both zoophily and indoor resting was An. vagus, which demonstrated both anthropophily and high resting rates indoors-pointing to this species being a possible primary vector at this site. CONCLUSION: A diverse Anopheles fauna in Bandarban has been confirmed through molecular methods, highlighting the potential impact of sampling techniques. Given the complexity of the local ecosystem, a better understanding of mosquito behaviour and ecology is required to achieve the goal of malaria elimination in Bangladesh.

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Insects use subtle and complex visual systems to capture spectral information and guide life activities. Spectral sensitivity of insect describes the relationship between the threshold of insect response to light stimulation and wavelength, which is the physiological basis and necessary condition for the generation of sensitive wavelength. The sensitive wavelength is the light wave with the strong reaction at the physiological or behavioral level of insects, which is the special and specific manifestation of spectral sensitivity. Understanding the physiological basis of insect spectral sensitivity can effectively guide the determination of sensitive wavelength. In this review, we summarized the physiological basis of insect spectral sensitivity, analyzed the intrinsic influence of each link in the photosensitive process on spectral sensitivity, and summarized and compared the measurement methods and research results about the sensitive wavelength of different insect species. The optimal scheme of the sensitive wavelength measurement based on the analysis of the key influencing factors can provide references for the development and improvement of light trapping and control technology. Finally, we proposed that neurological research of spectral sensitivity of insect should be strengthened in the future.

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Usutu virus (USUV) is an emergent arbovirus in Europe causing mortality in bird populations. Similar to West Nile virus (WNV), USUV is maintained in sylvatic cycles between mosquito vectors and bird reservoirs. Spillover events may result in human neurological infection cases. Apart from indirect evidence provided by a recent serological study in wild birds, the circulation of USUV in Romania was not assessed. We aimed to detect and molecular characterize USUV circulating in mosquito vectors collected in South-Eastern Romania-a well-known WNV endemic region-during four transmission seasons. Mosquitoes were collected from Bucharest metropolitan area and Danube Delta, pooled, and screened by real-time RT-PCR for USUV. Partial genomic sequences were obtained and used for phylogeny. USUV was detected in Culex pipiens s.l. female mosquitoes collected in Bucharest, in 2019. The virus belonged to Europe 2 lineage, sub-lineage EU2-A. Phylogenetic analysis revealed high similarity with isolates infecting mosquito vectors, birds, and humans in Europe starting with 2009, all sharing common origin in Northern Italy. To our knowledge, this is the first study characterizing a strain of USUV circulating in Romania.

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Phlebotomine sand flies are responsible for the transmission of different pathogens, including flagellate protozoan of the genus Leishmania. To locate a vertebrate host, females use chemical cues called kairomones, which are also used by males and females to locate carbohydrate sources of food. In addition to these chemical cues, visual cues (such as light and color) can play a key role in determining host location. In this way, incandescent white light is widely used to capture these insects in the field, being more recently replaced by light-emitting diode (LEDs) technology, which can be used with different colors. In this review, we summarize the main results of the use of LEDs as attractants for sand flies. Considering the different LEDs colors, blue and green are responsible for catching the highest number of sand fly species, especially for field trials conducted in New World countries. The intensity of light colors is an important factor to be considered, but so far little explored. In general, the use of LEDs can be considered as a feasible tool for the improvement of light traps. Future research is required to assert the contribution of different colors individually, in different combinations and intensities, as well as the possible synergistic effect of LEDs in association with attractive compounds in the behavior of sand flies.

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Parallel to the widespread decline of plants and animals, there is also an ongoing expansion of many species, which is especially pronounced in certain taxonomic groups and in northern latitudes. In order to inform an improved understanding of population dynamics in range-expanding taxa, we studied species richness, abundance and population growth in a sample of 25,138 individuals representing 107 range-expanding moth species at three light-trap sites in southeastern Sweden over 16 years (from 2005 to 2020) in relation to temperature and years since colonisation. Species richness and average abundance across range-expanding moths increased significantly over time, indicating a continuous influx of species expanding their ranges northward. Furthermore, average abundance and population growth increased significantly with increasing average ambient air temperature during the recording year, and average abundance also increased significantly with increasing temperature during the previous year. In general, population growth increased between years (growth rate > 1), although the population growth rate decreased significantly in association with years since colonisation. These findings highlight that, in contrast to several other studies in different parts of the world, species richness and abundance have increased in southeastern Sweden, partly because the warming climate enables range-expanding moths to realise their capacity for rapid distribution shifts and population growth. This may lead to fast and dramatic changes in community composition, with consequences for species interactions and the functioning of ecosystems. These findings are also of applied relevance for agriculture and forestry in that they can help to forecast the impacts of future invasive pest species.

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BACKGROUND: Methods used to sample mosquitoes are important to consider when estimating entomologic metrics. Human landing catches (HLCs) are considered the gold standard for collecting malaria vectors. However, HLCs are labour intensive, can expose collectors to transmission risk, and are difficult to implement at scale. This study compared alternative methods to HLCs for collecting Anopheles mosquitoes in eastern Uganda. METHODS: Between June and November 2021, mosquitoes were collected from randomly selected households in three parishes in Tororo and Busia districts. Mosquitoes were collected indoors and outdoors using HLCs in 16 households every 4 weeks. Additional collections were done indoors with prokopack aspirators, and outdoors with pit traps, in these 16 households every 2 weeks. CDC light trap collections were done indoors in 80 households every 4 weeks. Female Anopheles mosquitoes were identified morphologically and Anopheles gambiae sensu lato were speciated using PCR. Plasmodium falciparum sporozoite testing was done with ELISA. RESULTS: Overall, 4,891 female Anopheles were collected, including 3,318 indoors and 1,573 outdoors. Compared to indoor HLCs, vector density (mosquitoes per unit collection) was lower using CDC light traps (4.24 vs 2.96, density ratio [DR] 0.70, 95% CIs 0.63-0.77, p < 0.001) and prokopacks (4.24 vs 1.82, DR 0.43, 95% CIs 0.37-0.49, p < 0.001). Sporozoite rates were similar between indoor methods, although precision was limited. Compared to outdoor HLCs, vector density was higher using pit trap collections (3.53 vs 6.43, DR 1.82, 95% CIs 1.61-2.05, p < 0.001), while the sporozoite rate was lower (0.018 vs 0.004, rate ratio [RR] 0.23, 95% CIs 0.07-0.75, p = 0.008). Prokopacks collected a higher proportion of Anopheles funestus (75.0%) than indoor HLCs (25.8%), while pit traps collected a higher proportion of Anopheles arabiensis (84.3%) than outdoor HLCs (36.9%). CONCLUSION: In this setting, the density and species of mosquitoes collected with alternative methods varied, reflecting the feeding and resting characteristics of the common vectors and the different collection approaches. These differences could impact on the accuracy of entomological indicators and estimates of malaria transmission, when using the alternative methods for sampling mosquitos, as compared to HLCs.

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The fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae), is an important migratory pest, causing great losses to agricultural production. Light trapping is a pesticide-free method for pest control and is influenced by many factors, especially wavelength and light intensity. In this study, a series of phototactic behavioral assays were carried out and the physical parameters were included to identify phototactic responses of S. frugiperda, with Helicoverpa armigera as control. It was found that S. frugiperda showed the highest average phototactic rate to blue light among five different LED lights. The phototactic rates of the two moths increased gradually with light intensity and were not obviously influenced by sex. In addition, the phototactic rate of S. frugiperda was significantly lower under a low light intensity of UV light than that of H. armigera, further confirmed by the indoor simulation experiment and EC50. According to the obtained parameters, the trapping distance of S. frugiperda to blue light was smaller than that of H. armigera to UV light. Therefore, we summarized a proposal of using blue light for light traps to control S. frugiperda, with a maximum distance of no more than 108 m. These results provide an experimental and theoretical basis for improving light-trapping techniques for managing S. frugiperda.

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Mosquito control programs in the State of Florida are charged with protecting human and animal health, fostering economic development of the State, permitting enjoyment of the natural attractions in Florida, and improving the quality of life of citizens. Mosquito control programs must accomplish these tasks in such a manner as will protect the environment and terrestrial, marine, and freshwater ecosystems. The Florida Keys Mosquito Control District provides a science-based Integrated Pest Management mosquito control program to the residents of the Florida Keys, Monroe County, Florida. Operational decisions are based on surveillance of adult and immature mosquitoes. Mosquito populations are monitored by means of carbon dioxide-baited light traps BG Sentinel traps, truck traps, gravid traps, oviposition traps, and human landing rate counts. Larvae and pupae are monitored by inspections of natural and human-made immature habitats. Due to past and current reliance on chemical pesticides for control of mosquitoes, the District maintains a pesticide resistance detection program consisting of CDC bottle bioassays and larval bioassays, challenging local mosquito species with currently used adulticides and larvicides.

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Mosquitoes (Culicidae) are ubiquitous flying insects that function as vectors for several viruses that cause disease in humans. Mosquito abundance and diversity are influenced by landscape features and environmental factors such as temperature and precipitation and vary across seasons and years. The range and phenology of many mosquito species that vector viruses relevant to human health are changing. We sampled mosquito communities in central Oklahoma for four years at thirteen sites, collecting over 25,000 mosquitoes; among these, we identified 27 different species, including several that transmit human pathogens and were collected in suburban backyards. Community composition differed across the landscape and changed from early season to late season and year to year. This effort to describe mosquito communities in Oklahoma is a first step toward assessing and predicting arbovirus risk, an ongoing and dynamic public health challenge.

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Aggregation of flying insects such as gypsy moths at commercial light sources in summer not only has an aesthetically negative impact on public facilities but also permits the establishment of new insect populations there from the next year. Although energy-efficient light traps equipped with light-emitting diodes (LEDs) have recently been used for controlling pest insects in agriculture, there are very few maintenance-free light traps that are available on the market. Based on the results of field surveys, we fabricated a prototype light trap in which the preferences of insects for light irradiation angle and wavelength are implemented. Field experiments revealed that flying moths were attracted more to light with a narrow irradiation angle than to light with a wide irradiation angle. Moreover, there was a tendency for fewer moths to be collected when fluorescent paint was applied to the surface of the flight-interception board, indicating that a high contrast made by illumination and the background is preferred by flying moths. Taken together with our previous results, we found that the moth catch was influenced more by modification of the light design than by change in visible light wavelengths. A semi-portable light trap, named the "Kurihara trap" after the primary contributor to its development, is made of light-weight plastic and is driven by solar power. This light trap is omnidirectional and maintenance-free and is therefore suitable for deployment in the backyards of rest areas as well as at houses for long-term macromoth sampling.

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Using collection methods for Aedes adults as surveillance tools provides reliable indices and arbovirus detection possibilities. This study compared the effectiveness of different methods for collecting Ae. aegypti and Ae. albopictus and detecting arboviruses circulating in field-caught female specimens. Collection sites were defined in urban, peri-urban, and rural landscapes in two Brazilian cities. Collections were performed using Adultraps (ADT), BG-Sentinel (BGS), CDC-like traps (CDC), and indoor (ASP-I) and outdoor (ASP-O) aspiration during the rainy and dry seasons of 2015 and 2016. Generalized linear mixed models were used to model the effectiveness of each collection method. A total of 434 Ae. aegypti and 393 Ae. albopictus were collected. In total, 64 Ae. aegypti and sixteen Ae. albopictus female pools were tested for DENV, CHIKV, ZIKV, or YFV; none were positive. Positivity and density were linear at low densities (<1 specimen); thereafter, the relationship became non-linear. For Ae. aegypti, ADT and CDC were less effective, and ASP-I and ASP-O were as effective as BGS. For Ae. albopictus, all collection methods were less effective than BGS. This study highlights the need for an integrated surveillance method as an effective tool for monitoring Aedes vectors.