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Exp Parasitol ; 222: 108062, 2021 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-33383024

RESUMEN

Long non-coding RNAs (lncRNAs) perform several types of regulatory functions and have been recently explored in the genus Schistosoma. Although sequencing and bioinformatics approaches have demonstrated the presence of hundreds of lncRNAs and microRNAs (miRNAs) in this genus, information regarding their abundance, characteristics, and potential functions linked to Schistosoma mansoni biology and parasite-host interaction is limited. Our objectives in the present study were to verify whether 15 previously identified S. mansoni lncRNAs are detectable in the host liver. In addition, we assess whether these lncRNAs are present in the S. mansoni infective form and the stages inside the definitive host. The detection of these 15 S. mansoni lncRNAs and a long terminal repeat (LTR) retrotransposon Saci 4 was performed in the eggs, cercariae, and 3.5-h schistosomula. All lncRNAs were found to be expressed in these stages; some of the lncRNAs were found in the livers of the infected C57BL/6 mice. In conclusion, S. mansoni lncRNAs were detected in host livers and quantified. Furthermore, many of the lncRNAs analyzed showed differential expression in the larval stages, indicating that they play a stage-specific regulatory role.


Asunto(s)
Hígado/parasitología , ARN Largo no Codificante/aislamiento & purificación , Schistosoma mansoni/genética , Esquistosomiasis mansoni/parasitología , Animales , Mapeo Cromosómico , Hígado/patología , Masculino , Ratones , Ratones Endogámicos C57BL , Reacción en Cadena en Tiempo Real de la Polimerasa , Retroelementos/fisiología , Transcripción Reversa , Schistosoma mansoni/crecimiento & desarrollo , Schistosoma mansoni/aislamiento & purificación , Esquistosomiasis mansoni/patología
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