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Symbionts dominate planetary diversity and three primary symbiont diversification processes have been proposed: co-speciation with hosts, speciation by host-switching, and within-host speciation. The last mechanism is prevalent among members of an extraordinary marine symbiosis in the Indian River Lagoon, Florida, composed of a host mantis shrimp, Lysiosquilla scabricauda, and seven host-specific commensal vasconielline "yoyo" clams (Galeommatoidea) that collectively occupy two distinct niches: burrow-wall-attached, and host-attached/ectocommensal. This within-host symbiont radiation provides a natural experiment to test how symbiont coexistence patterns are regulated in a common ancestral habitat. The competitive exclusion principle predicts that sister taxa produced by adaptive speciation (with distinct morphologies and within-burrow niches) are most likely to coexist whereas the neutral theory predicts no difference among adaptive and non-adaptive sister taxa co-occurrence. To test these predictions, we engaged in (1) field-censusing commensal species assemblages; (2) trophic niche analyses; (3) laboratory behavioral observations. Although predicted by both models, the field census found no mixed-niche commensal assemblages: multi-species burrows were exclusively composed of burrow-wall commensals. Their co-occurrence matched random assembly process expectations, but presence of the single ectocommensal species had a highly significant negative effect on recruitment of all burrow-wall commensal species (P < 0.001), including on its burrow-wall commensal sister species (P < 0.001). Our stable isotope data indicated that commensals are suspension feeders and that co-occurring burrow-wall commensals may exhibit trophic niche differentiation. The artificial burrow behavioral experiment yielded no evidence of spatial segregation among burrow-wall commensals, and it was terminated by a sudden breakdown of the host-commensal relationship resulting in a mass mortality of all commensals unattached to the host. This study system appears to contain two distinct, superimposed patterns of commensal distribution: (1) all burrow-wall commensal species; (2) the ectocommensal species. Burrow-wall commensals (the plesiomorphic condition) broadly adhere to neutral theory expectations of species assembly but the adaptive evolution of ectocommensalism has apparently led to ecological exclusion rather than coexistence, an inverse outcome of theoretical expectations. The ecological factors regulating the observed burrow-wall/ectocommensal exclusion are currently obscure but potentially include differential recruitment to host burrows and/or differential survival in "mixed" burrow assemblages, the latter potentially due to changes in host predatory behavior. Resampling host burrows during commensal recruitment peak periods and tracking burrow-wall commensal survival in host burrows with and without added ectocommensals could resolve this outstanding issue.

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The kleptoparasitic pea crab Zaops ostreum lives within the gills of bivalves, including the economically important eastern oyster Crassostrea virginica. Previous research along the east coast of central Florida has found an average of 2.3 pieces of plastic per oyster. The goals of our research were to determine if filter-feeding oysters transfer microfibers to Z. ostreum via the crab: 1) actively consuming plastic particles, or 2) passively becoming entangled in microfibers. Our results show that both occur. While only 11.6 % of Z. ostreum (total n = 122) consumed microfibers, those that did had up to 14 pieces in their soft tissues. Similarly, only 7.4 % of Z. ostreum had microfibers entangled around their appendages. Mean lengths of consumed and entangled fibers were similar, 1.9 and 2.7 mm, respectively. Additional research is needed to understand the positive and negative impacts of microfibers associated with pea crabs on both species.

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Mortality patterns in cetaceans are critical to understanding population health. Common bottlenose dolphins (Tursiops truncatus truncatus) inhabiting the Indian River Lagoon (IRL), Florida have been subjected to four unusual mortality events (UMEs), highlighting the need to evaluate morbidity and mortality patterns. Complete gross examinations were conducted on 392 stranded dolphins and histopathological analyses were conducted for 178 animals (2002-2020). The probable causes of mortality were grouped by etiologic category: degenerative, metabolic, nutritional, inflammatory (infectious and noninfectious disease), and trauma. Probable cause of mortality was determined in 57% (223/392) of cases. Inflammatory disease (infectious/noninfectious) and trauma were the most common. Inflammatory disease accounted for 41% of cases (91/223), with the lungs (pneumonia) most commonly affected. Trauma accounted for 36% of strandings (80/223). The majority of trauma cases were due to anthropogenic activities (entanglement, fishing gear or other debris ingestion, and propeller strikes), accounting for 58% of trauma cases (46/80). Natural trauma (prey-associated esophageal obstruction or asphyxiation, shark bites, and stingray interactions) accounted for 12% of all cases (26/223), and trauma of undetermined origin was identified in 4% of cases (8/223). Starvation or inanition (nutritional) were the probable cause of mortality in 17% of cases and peaked during the 2013 UME (61% of cases). Degenerative and metabolic etiologies accounted for 5% of cases. This study represents the most comprehensive evaluation of morbidity and mortality patterns in IRL dolphins. Because IRL dolphins are routinely exposed to anthropogenic threats and have endured multiple UMEs, these baseline data are critical to the conservation and management of this population.

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The Indian River Lagoon (IRL) spans approximately one-third of the east coast of Florida and, in recent years, has faced frequent harmful algal blooms (HABs). Blooms of the potentially toxic diatom, Pseudo-nitzschia, occur throughout the lagoon and were reported primarily from the northern IRL. The goal of this study was to identify species of Pseudo-nitzschia and characterize their bloom dynamics in the southern IRL system where monitoring has been less frequent. Surface water samples collected from five locations between October 2018 and May 2020 had Pseudo-nitzschia spp. present in 87% of samples at cell concentrations up to 1.9×103 cells mL-1. Concurrent environmental data showed Pseudo-nitzschia spp. were associated with relatively high salinity waters and cool temperatures. Six species of Pseudo-nitzschia were isolated, cultured, and characterized through 18S Sanger sequencing and scanning electron microscopy. All isolates demonstrated toxicity and domoic acid (DA) was present in 47% of surface water samples. We report the first known occurrence of P. micropora and P. fraudulenta in the IRL, and the first known DA production from P. micropora.

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Meteoric groundwater discharge (MGD) to coastal regions transports terrestrial freshwater and nutrients that may alter coastal ecosystems by supporting harmful algal blooms. Estimation of MGD-driven nutrients is crucial to assess potential effects on coastal zones. These estimates require a reliable assessment of MGD rates and pore water nutrient concentrations below subterranean estuaries. To estimate nutrient delivery into a subterranean estuary in the Indian River Lagoon, FL., pore water and surface water samples were collected from nested piezometers along a selected transect on five sampling episodes. Groundwater hydraulic head and salinity were measured in thirteen onshore and offshore piezometers. Numerical models were developed, calibrated, and validated using SEAWAT to simulate MGD flow rates. Lagoon surface water salinity exhibits no spatial but mild temporal variation between 21 and 31. Pore water salinity shows tremendous variation in time and space throughout the transect except in the middle region of the lagoon which exhibits uniform but elevated salinities up to 40. Pore water salinity as low as that of freshwater happens to occur in the shoreline regions during most of the sampling episodes. Both pore water and surface water show remarkably higher total nitrogen TN than total phosphorus TP concentrations and most TN is exported as NH4, reflecting the effect of mangroves on the geochemical reactions that reduce NO3 into NH4. Nutrient contributions of pore water and lagoon water exceed the Redfield TN/TP molar ratio in all sampling trips by up to a factor of 48 and 4, respectively. Estimated TP and TN fluxes receives by the lagoon via MGD are 41-106 and 113-1478 mg/d/m of shoreline. The molar TN/TP ratio of nutrient fluxes exceeds the Redfield ratio by a factor of up to 3.5 which indicates the potential of MGD-driven nutrients to alter the lagoon water quality and support harmful algal blooms.

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Microplastics (MP) are a pervasive environmental pollutant that enter coastal water bodies, posing an ingestion risk to marine biota. This study quantified the ability of the Eastern oyster (Crassostrea virginica) to egest MP in-situ in their biodeposits - feces and pseudofeces. Oysters of all sizes were able to egest environmental MP at a mean rate of 1 MP per 1 h through feces, and 1 MP per 2 h through pseudofeces. Smaller C. virginica were more efficient at egesting MP, and efficiency decreased by 0.8% per 1-g increase in tissue weight, with C. virginica of harvestable size being much less efficient. These findings are of relevance to resource managers for C. virginica populations as it further contributes to our understanding of MP accumulation in wild populations and has implications for not just C. virginica but also for their consumers.

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A hydrodynamic model is applied to a domain encompassing Mosquito Lagoon and a segment of north Indian River Lagoon on the east coast of Florida with the goals of (1) describing the hydrodynamic regime of the area according to existing conditions and (2) to determine the flushing rates of the system after introducing a hypothetical pollutant (numerical dye tracer). Results from the 1-year simulation agree with observations and show that the Mosquito Lagoon (ML) and North Indian River Lagoon (NIRL) are dominated by the nontidal (low frequency) water level oscillations except at the northernmost part of the ML where the influence of tidal renewal dominates. The global tracer application results reveal that only the two northernmost segments of Mosquito Lagoon (ML-ac and ML-df) reach 50% of tracer concentrations (R50) within the year of simulation (day 19 and day 176 respectively). The five (discrete) segments tracer study resulted in lower R50 values and the hypothetical pollutant is removed (flushed) from each of the segments within the year and with an averaged renewal time value for the Mosquito Lagoon basin of 98 days. Observed throughout the simulation period is the interaction across the different segments and a predominantly two-layered water mass exchange through the Haulover Canal. Most significant is that the resulting renewal times (R50) indicate the existence of three dynamically distinct sections within Mosquito Lagoon. These findings aid in the identification of poorly flushed zones and serve as a baseline for the duration and possible propagation of detrimental conditions such as a harmful algal bloom (HAB) event or a contaminant spill.

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Fibropapillomatosis (FP) is a tumorous disease affecting all species of sea turtles and is associated with the pathogen chelonid alphaherpesvirus 5 (ChHV5). Hypothesized ChHV5 vectors include the marine leeches Ozobranchus branchiatus and O. margoi, but data on their associations with FP and ChHV5 are minimal. To establish relationships between leech parasitism, turtle hosts, and FP, we compared green and loggerhead turtles from the Indian River Lagoon (IRL), Florida, USA, in terms of (1) the presence or absence of ChHV5 within associated leeches, (2) the association between leech parasitism and host FP status, and (3) seasonal variation in leech presence. We identified 55 leeches collected from green turtles as O. branchiatus and 22 leeches collected from loggerhead turtles as O. margoi. Of 77 sequenced leeches, 10 O. branchiatus and 5 O. margoi were ChHV5 positive. ChHV5-positive O. branchiatus trended towards coming from FP-positive hosts. Using 12 yr of turtle capture data from the IRL, we found that leech parasitism was significantly correlated with FP and capture month in green turtles but not in loggerhead turtles. These results suggest that O. branchiatus and O. margoi may differ in their ability to transmit ChHV5 or to encounter and remain on FP-positive hosts. Alternatively, potential immunological differences between green and loggerhead turtles may explain the observed relationships. This study is the first to provide robust statistical evidence of an association between leeches and FP, as well as seasonal fluctuations in leech presence, in green turtles but not in loggerhead turtles.

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We report 24 new records of the Brazilian cownose ray Rhinoptera brasiliensis outside its accepted geographic range. Sequencing of a 442-base pair portion of the mitochondrial NADH dehydrogenase subunit 2 gene for 282 Rhinoptera samples revealed eight records off the east coast of the USA and 16 from the eastern Gulf of Mexico. Both sexes of all life stages were documented in all seasons over multiple years in the Indian River and Lake Worth lagoons, Florida, indicating that their range extends further in the western North Atlantic than previously described.

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Harmful algal bloom (HAB) toxins have severe negative impacts on marine mammals, particularly for Florida bottlenose dolphins (Tursiops truncatus) which frequently experience mass mortality events. Dolphins on the Florida Atlantic coast inhabit a region endemic to two HAB species, Karenia brevis and Pyrodinium bahamense, which produce the neurotoxins brevetoxin (PbTx) and saxitoxin (STX), respectively. Although toxic HABs and associated dolphin mortality events have been reported from this region, there is a lack of available data necessary for comparing toxin exposure levels between bloom ('exposed') conditions and non-bloom ('baseline') conditions. Here we present a 10-year dataset of PbTx and STX concentrations detected in dolphins stranding in this region, and compare the toxin loads from HAB-exposed dolphins to those detected in dolphins recovered in the absence of a HAB. We analyzed liver tissue samples from dead-stranded dolphins (n = 119) recovered and necropsied between 2002-2011, using an enzyme-linked immunosorbent assay (ELISA) modified for use with mammalian tissues. For dolphins recovered during baseline conditions, toxin-positive samples ranged in concentration from 0.27 to 1.2 ng/g for PbTx and from 0.41 to 1.9 ng/g for STX. For K. brevis-exposed dolphins, concentrations of up to 12.1 ng PbTx/g were detected, and for P. bahamense-exposed dolphins, concentrations of up to 9.9 ng STX/g were detected. Baseline PbTx values were similar to those reported in other regions where K. brevis blooms are more frequent and severe, but HAB-exposed PbTx values were considerably lower relative to these other regions. Since no baseline STX dolphin data exist for any region, our data serve as a first step towards establishing reference STX values for potential dolphin mortality events associated with STX-producing blooms in the future. This study demonstrates that although HABs in eastern Florida are only infrequently associated with dolphin mortalities, the presence of toxins in these animals may pose significant health risks in this region.

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Redfin needlefish Strongylura notata from Florida coastal waters were observed with unusual neoplastic lesions. Affected specimens were collected from 1 Atlantic estuary (Indian River Lagoon, prevalence = 0.32%, n = 5314) and 2 Gulf of Mexico estuaries (Tampa Bay, prevalence = 0.02%, n = 10762; Charlotte Harbor, prevalence = 0.02%, n = 5112) during routine fisheries-independent monitoring surveys conducted from 1999-2009. Grossly, each lesion manifested as a large (18-30 mm × 20-50 mm), raised (approximately 10 mm), white, creamy, or pinkish nodule on the flank, dorsal trunk, base of the pectoral fin, or head. Multiple small (<5 mm) nodules possessing poorly demarcated borders with neighboring tissues on the external jaw surface and at the base of the teeth were also observed. Histopathologically, neoplastic cells were found in the dermis, beneath the skeletal muscle, and in the soft tissue at the base of teeth of the premaxilla and the dentary jaw processes. Neoplastic cells usually had prominently invaded among the myosepta of the skeletal muscle. Neoplastic parenchymal cells had the basic characteristics of atypical, mononuclear, round, histiocytic cells with an eccentric, reniform nucleus and abundant cytoplasmic vacuolation, while some exhibited bizarre nuclear pleomorphism. Transmission electron microscopy revealed that neoplastic cells had a grooved nucleus and cytoplasmic organelles with rough endoplasmic reticulum, mitochondria, Golgi apparatus, and lysosomes. Neoplastic cells had possibly metastasized to liver, spleen, and kidney. Positive immunohistochemical staining with Ki67, p53, S-100, and CD163 support neoplastic features and a putative diagnosis of histiocytic sarcoma.

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The success of many sessile invertebrates in marine benthic communities is linked to their ability to efficiently remove suspended organic matter from the surrounding water column. To investigate the diet of the barnacle Amphibalanus eburneus, a dominant suspension feeder within the Indian River Lagoon (IRL) of central Florida, we compared the stable isotopes ratios (δ13C and δ15N) of barnacle tissue to those of particulate organic matter (POM). Collections were carried out quarterly for a year from 29 permanent sites and at sites impacted by an Aureoumbra lagunensis bloom. δ13C and δ15N values of Amphibalanus eburneus varied across sites, but δ15N was more stable over time. There was a range of δ15N values of Amphibalanus eburneus tissue from 6.0‰ to 10.5‰ across sites. Because land-based sources such as sewage are generally enriched in 15N, this suggests a continuum of anthropogenic influence across sites in the IRL. Over 70% of the variation in δ15N values of Amphibalanus eburneus across sites was driven by the δ15N values of POM, supporting a generalist feeding strategy on available sources of suspended organic matter. The dominance of this generalist consumer in the IRL may be linked to its ability to consume spatially and temporally variable food resources derived from natural and anthropogenic sources, as well as Aureoumbra lagunensis cells. Generalist consumers such as Amphibalanus eburneus serve an important ecological role in this ecosystem and act as a sentinel species and recorder of local, site-specific isotopic baselines.

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This study determined the quantity and diversity of microplastics in water and soft tissues of eastern oysters (Crassostrea virginica) and Atlantic mud crabs (Panopeus herbstii) in Mosquito Lagoon, a shallow, microtidal estuary along the east coast of central Florida. One-liter water samples had an average of 23.1 microplastic pieces (n = 15). Crabs (n = 90) had an average of 4.2 pieces in tissues/individual plus an average of 20.3 pieces/individual temporarily entangled in exposed surfaces and released within 5 days in tanks. Adult oysters (n = 90) had an average of 16.5 microplastic pieces/individual. Fibers, mostly royal/dark blue in color, dominated our collections. When compared per gram of tissue, crabs had two orders of magnitude more microplastic pieces than oysters. Our numbers were higher than previous studies on invertebrate microplastics; this is potentially the result of extensive urbanization, limited flushing, and intensive recreational usage of Mosquito Lagoon.

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The Indian River Lagoon (IRL) system, a poorly flushed 240 km long estuary in east-central Florida (USA), previously received 200 MLD of point source municipal wastewater that was largely mitigated by the mid-1990's. Since then, non-point source loads, including septic tank effluent, have become more important. Seventy sites were sampled for bloom-forming macroalgae and analyzed for δ15N, % nitrogen, % phosphorus, carbon:nitrogen, carbon:phosphorus, and nitrogen:phosphorus ratios. Data were fitted to geospatial models showing elevated δ15N values (>+5‰), matching human wastewater in most of the IRL system, with elevated enrichment (δ15N ≥ +7‰ to +10‰) in urbanized portions of the central IRL and Banana River Lagoon. Results suggest increased mobilization of OSDS NH4+ during the wetter 2014 season. Resource managers must improve municipal wastewater treatment infrastructure and commence significant septic-to-sewer conversion to mitigate nitrogen over-enrichment, water quality decline and habitat loss as mandated in the Tampa and Sarasota Bays and the Florida Keys.

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Understanding anthropogenic and hydro-climatic influences on nutrient concentrations and export from highly managed catchments often necessitates trend detection using long-term monitoring data. This study analyzed the temporal trend (1979-2014) of total nitrogen (TN) and total phosphorus (TP) concentrations and export from four adjacent coastal basins in south Florida where land and water resources are highly managed through an intricate canal network. The method of integrated seasonal-trend decomposition using LOESS (LOcally weighted regrESSion) was employed for trend detection. The results indicated that long-term trends in TN and TP concentrations (increasing/decreasing) varied with basins and nutrient species, reflecting the influence of basin specific land and water management practices. These long-term trends were intervened by short-term highs driven by high rainfall and discharges and lows associated with regional droughts. Seasonal variations in TP were more apparent than for TN. Nutrient export exhibited a chemostatic behavior for TN from all the basins, largely due to the biogenic nature of organic N associated with the ubiquity of organic materials in the managed canal network. Varying degrees of chemodynamic export was present for TP, reflecting complex biogeochemical responses to the legacy of long-term fertilization, low soil P holding capacity, and intensive stormwater management. The anthropogenic and hydro-climatic influences on nutrient concentration and export behavior had great implications in nutrient loading abatement strategies for aquatic ecosystem restoration of the downstream receiving waterbody.

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Cyanobacterial blooms have been increasing worldwide due to increased nutrients associated with urban, industrial, and agricultural development. Blooms that occur in the Indian River Lagoon (IRL), Florida may be increased by nutrient-laden runoff from storm water and non-point sewage pollution due to alterations to the watershed. In the IRL, during the summer of 2006, extensive blooms of the marine cyanobacterium, Lyngbya majuscula, were observed forming mats throughout beds of the seagrass Halodule wrightii in Fort Pierce, Florida. The effects of cyanobacterial blooms were compared to artificial shading of H. wrightii to assess the shading potential of L. majuscula. The combined effects of L. majuscula removal and artificial shading showed increases in the below ground biomass of H. wrightii. However, leaf length increased in the presence of L. majuscula. In response to artificial shading, H. wrightii decreased in density, but showed similar leaf elongation. A common bivalve in our study area, Macoma constricta, increased in density when L. majuscula was removed. Therefore, when L. majuscula blooms occur, light limitation is one of the mechanisms altering H. wrightii density and leaf lengths in the IRL. Loss of H. wrightii biomass due to shading from cyanobacterial mats may further damage the diversity and habitat value of the IRL.

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Seagrasses are the foundation of many coastal ecosystems and are in global decline because of anthropogenic impacts. For the Indian River Lagoon (Florida, U.S.A.), we developed competing multistate statistical models to quantify how environmental factors (surrounding land use, water depth, and time [year]) influenced the variability of seagrass state dynamics from 2003 to 2014 while accounting for time-specific detection probabilities that quantified our ability to determine seagrass state at particular locations and times. We classified seagrass states (presence or absence) at 764 points with geographic information system maps for years when seagrass maps were available and with aerial photographs when seagrass maps were not available. We used 4 categories (all conservation, mostly conservation, mostly urban, urban) to describe surrounding land use within sections of lagoonal waters, usually demarcated by land features that constricted these waters. The best models predicted that surrounding land use, depth, and year would affect transition and detection probabilities. Sections of the lagoon bordered by urban areas had the least stable seagrass beds and lowest detection probabilities, especially after a catastrophic seagrass die-off linked to an algal bloom. Sections of the lagoon bordered by conservation lands had the most stable seagrass beds, which supports watershed conservation efforts. Our results show that a multistate approach can empirically estimate state-transition probabilities as functions of environmental factors while accounting for state-dependent differences in seagrass detection probabilities as part of the overall statistical inference procedure.

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Bottlenose dolphins (Tursiops truncatus) inhabiting the Indian River Lagoon, FL (IRL) have tissue mercury concentrations among the highest reported worldwide. Analysis of total mercury (THg) concentrations in blood collected between 2003 and 2012 showed a significant linear decrease over time (p=0.04). Significant differences in the spatial distribution of THg in resident IRL dolphins were also observed with a general gradient in concentration from north to south. Evaluation of local biogeochemistry and accumulation of mercury in prey species is needed to better understand factors influencing the distribution of Hg in the apex predator. Analyses of temporal and spatial patterns of exposure to THg in this sentinel species may have implications for both ecosystem and public health in the region.

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Estuaries are important ecosystems for mercury methylation and accumulation, yet few studies have quantified mercury levels in prey species that expose large estuarine piscivores to contamination. We measured mercury concentrations in key prey species from the Indian River Lagoon, Florida, USA, to characterize variability and assess the spatial distribution of mercury levels throughout the estuary. Mean mercury concentrations ranged from 0.015mg/kg in Mugil cephalus to 0.222mg/kg in Bairdiella chrysoura and varied considerably in some species. Spatial variation was observed within species, but a universal geographic pattern among species was not apparent, likely reflecting the broad habitat-use patterns associated with the estuarine-transient life histories of many of these common prey species. In contrast, estuarine-resident species, such as B. chrysoura, may be useful indicators of regional mercury methylation potential and bioaccumulation in biota. These results can advance future studies that relate dietary mercury uptake and mercury level variations in estuarine piscivores.

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Globally distributed, the bottlenose dolphin (Tursiops truncatus) is found in a range of offshore and coastal habitats. Using 15 microsatellite loci and mtDNA control region sequences, we investigated patterns of genetic differentiation among putative populations along the eastern US shoreline (the Indian River Lagoon, Florida, and Charleston Harbor, South Carolina) (microsatellite analyses: n = 125, mtDNA analyses: n = 132). We further utilized the mtDNA to compare these populations with those from the Northwest Atlantic, Gulf of Mexico, and Caribbean. Results showed strong differentiation among inshore, alongshore, and offshore habitats (ФST = 0.744). In addition, Bayesian clustering analyses revealed the presence of 2 genetic clusters (populations) within the 250 km Indian River Lagoon. Habitat heterogeneity is likely an important force diversifying bottlenose dolphin populations through its influence on social behavior and foraging strategy. We propose that the spatial pattern of genetic variation within the lagoon reflects both its steep longitudinal transition of climate and also its historical discontinuity and recent connection as part of Intracoastal Waterway development. These findings have important management implications as they emphasize the role of habitat and the consequence of its modification in shaping bottlenose dolphin population structure and highlight the possibility of multiple management units existing in discrete inshore habitats along the entire eastern US shoreline.

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