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Clin Transl Oncol ; 24(11): 2210-2221, 2022 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-35842894

RESUMEN

OBJECTIVE: To investigate the effects of resveratrol (RSVL) on epithelial-mesenchymal transition (EMT) and biological behaviors of gastric cancer cells. METHODS: SGC-7901 cells were treated with RSVL, followed by TGF-ß1 treatment for induction of EMT. Cell proliferation was tested by MTT assay, migration and invasion by Transwell and scratch assays, and Hippo-YAP signaling pathway activation by immunofluorescence. The RNA and protein expressions of E-cadherin, Vimentin, N-cadherin, and Snail were detected by qPCR and Western blot. A tumor model was constructed to examine the effect of RSVL on gastric tumor growth. RESULTS: RSVL inhibited the migration, invasion, and growth of gastric cancer cells in concentration- and time-dependent manners. RSVL inhibited TGF-ß1-induced EMT of gastric cancer cells, which might relate to inactivation of the Hippo-YAP pathway. In the mouse tumor model, RSVL inhibited the EMT process by suppressing the Hippo-YAP pathway. CONCLUSION: RSVL inhibited EMT of gastric cancer cells probably by weakening the Hippo-YAP signaling pathway.


Asunto(s)
Transición Epitelial-Mesenquimal , Neoplasias Gástricas , Animales , Cadherinas/metabolismo , Línea Celular Tumoral , Movimiento Celular , Vía de Señalización Hippo , Ratones , Proteínas Serina-Treonina Quinasas , ARN , Resveratrol/farmacología , Neoplasias Gástricas/metabolismo , Factor de Crecimiento Transformador beta1/metabolismo , Vimentina/metabolismo , Proteínas Señalizadoras YAP
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