RESUMEN

Genetically Modified (GM) Organisms have been used in various domains since their introduction in the 1980s. According to ISAAA data, the use of GM crops in agriculture has also increased significantly in the past 30 years. However, even after 3 decades of commercialisation, GM crops are still surrounded with controversies with different countries adopting varying approaches to their introduction in the consumer markets, owing to different stances of various stakeholders. Motivated by this multitude of opinions, and absence of knowledge mapping, this study has undertaken scientometric analysis of the publication (Web of Science) and patent (Lens.org) data about genetically modified technology use in agriculture to explore the changing knowledge patterns and technological advancements in the area. It explores both scientific and technological perspectives regarding the use of Genetically Modified Crops, by using publication as well as patent data. The findings of this study highlight the major domains of research, technology development, and leading actors in the ecosystem. These findings can be helpful in taking effective policy decisions, and furthering the research activities. It presents a composite picture using both publications and patent data. Further, it will be of utility to explore the other technologies which are replacing GM technology in agriculture in future studies.

RESUMEN

A dual-emission fluorescent biosensing method was developed for simultaneous determination of CaMV35S and NOS in genetically modified (GM) plants. Two designed hairpin DNA (H1, H2) sequences were used as templates to synthesize H1-AgNCs (λex = 570 nm, λem = 625 nm) and H2-AgNCs (λex = 470 nm, λem = 555 nm). By using H1-AgNCs and H2-AgNCs as dual-signal tags, combined with signal amplification strategy of magnetic separation to reduce background signal and an enzyme-free catalytic hairpin assembly (CHA) signal amplification strategy, a novel multi-target fluorescent biosensor was fabricated to detect multiple targets based on FRET between signal tags (donors) and magnetic Fe3O4 modified graphene oxide (Fe3O4@GO, acceptors). In the presence of the target NOS and CaMV35S, the hairpin structures of H1 and H2 can be opened respectively, and the exposed sequences will hybridize with the G-rich hairpin sequences HP1 and HP2 respectively, displacing the target sequences to participate in the next round of CHA cycle. Meanwhile, H1-HP1 and H2-HP2 double-stranded DNA sequences (dsDNA) were formed, resulting in the desorption of dsDNA from the surface of Fe3O4@GO due to weak π-π interaction between dsDNA and Fe3O4@GO and leading to the fluorescence recovery of AgNCs. Under optimal conditions, the linear ranges of this fluorescence sensor were 5 ~ 300 nmol L-1 for NOS and 5 ~ 200 nmol L-1 CaMV35S, and the LODs were 0.14 nmol L-1 and 0.18 nmol L-1, respectively. In addition, the fluorescence sensor has good selectivity for the detection of NOS and CaMV35S in GM soybean samples, showing the potential applications in GM screening.

Asunto(s)

Técnicas Biosensibles , Límite de Detección , Nanopartículas del Metal , Plata , Plata/química , Técnicas Biosensibles/métodos , Nanopartículas del Metal/química , Transferencia Resonante de Energía de Fluorescencia/métodos , Grafito/química , Secuencias Invertidas Repetidas , Plantas Modificadas Genéticamente/genética , Catálisis , Colorantes Fluorescentes/química , Caulimovirus/genética , Técnicas de Amplificación de Ácido Nucleico/métodos , Proteínas Virales/química , Proteínas Virales/genética , Aminoácido Oxidorreductasas

RESUMEN

Insect-protected soybean (SIP) that produces the Cry1A.105 and Cry2Ab2 insecticidal crystal proteins has been developed to provide protection from feeding damage caused by targeted lepidopteran insect pests. Typically, as part of environmental risk assessment (ERA), plant characterization is conducted, and the data submitted to regulatory agencies prior to commercialization of genetically modified (GM) crops. The objectives of this research were to: (a) compare soybean with and without the SIP trait in plant characterization field trials designed to fulfill requirements for submissions to global regulatory agencies and address China-specific considerations and (b) compare risk assessment conclusions across regions and the methodologies used in the field trials. The soybean with and without the SIP trait in temperate, tropical, and subtropical germplasm were planted in replicated multi-location trials in the USA (in 2012 and 2018) and Brazil (in 2013/2014 and 2017/2018). Agronomic, phenotypic, plant competitiveness, and survival characteristics were assessed for soybean entries with and without the SIP trait. Regardless of genetic background, growing region, season, or testing methodology, the risk assessment conclusions were the same: the evaluated insect-protected soybean did not differ from conventional soybean in evaluated agronomic, phenotypic, competitiveness, and survival characteristics indicating no change in plant pest/weed potential. These results reinforce the concept of data transportability across global regions, different seasons, germplasm, and methodologies that should be considered when assessing environmental risks of GM crops.

Asunto(s)

Glycine max , Plantas Modificadas Genéticamente , Glycine max/genética , Glycine max/parasitología , Glycine max/crecimiento & desarrollo , Animales , Plantas Modificadas Genéticamente/genética , Plantas Modificadas Genéticamente/crecimiento & desarrollo , Endotoxinas/genética , Brasil , Control Biológico de Vectores , Proteínas Hemolisinas/genética , Productos Agrícolas/genética , Insectos/genética , Insectos/patogenicidad , Lepidópteros/patogenicidad , Lepidópteros/genética , Proteínas Bacterianas/genética , Toxinas de Bacillus thuringiensis/genética

RESUMEN

Using the amino acid sequences and analysis of selected known structures of Bt Cry toxins, Cry1Ab, Cry1Ac, Cry1Ah, Cry1B, Cry1C and Cry1F we specifically designed immunogens. After antibodies selection, broad-spectrum polyclonal antibodies (pAbs) and monoclonal antibody (namely 1A0-mAb) were obtained from rabbit and mouse, respectively. The produced pAbs displayed broad spectrum activity by recognizing Cry1 toxin, Cry2Aa, Cry2Ab and Cry3Aa with half maximal inhibitory concentration (IC50) values of 0.12-9.86 µg/mL. Similarly, 1A0-mAb showed broad spectrum activity, recognizing all of the above Cry protein (IC50 values of 4.66-20.46 µg/mL) with the exception of Cry2Aa. Using optimizations studies, 1A10-mAb was used as a capture antibody and pAbs as detection antibody. Double antibody sandwich enzyme-linked immunosorbent assays (DAS-ELISAs) were established for Cry1 toxin, Cry2Ab and Cry3Aa with the limit of detection (LOD) values of 2.36-36.37 ng/mL, respectively. The present DAS-ELISAs had good accuracy and precisions for the determination of Cry toxin spiked tap water, corn, rice, soybeans and soil samples. In conclusion, the present study has successfully obtained broad-spectrum pAbs and mAb. Furthermore, the generated pAbs- and mAb-based DAS-ELISAs protocol can potentially be used for the broad-spectrum monitoring of eight common subtypes of Bt Cry toxins residues in food and environmental samples.

Asunto(s)

Anticuerpos Monoclonales , Toxinas de Bacillus thuringiensis , Endotoxinas , Ensayo de Inmunoadsorción Enzimática , Proteínas Hemolisinas , Animales , Ensayo de Inmunoadsorción Enzimática/métodos , Conejos , Ratones , Endotoxinas/análisis , Endotoxinas/inmunología , Proteínas Hemolisinas/inmunología , Proteínas Hemolisinas/análisis , Proteínas Hemolisinas/química , Anticuerpos Monoclonales/inmunología , Anticuerpos Monoclonales/química , Proteínas Bacterianas/inmunología , Proteínas Bacterianas/química , Proteínas Bacterianas/análisis , Bacillus thuringiensis/química , Ratones Endogámicos BALB C

RESUMEN

Insect resistant genetically modified Bt cotton (containing a gene of Bacillus thuringiensis) has substantial potentiality of mounting cotton productivity. This study unveils an early insight on the economic viability of Bt cotton in Bangladesh. A total of 248 traditional cotton farmers and 8 Bt cotton experimental fields were surveyed in April 2022 for achieving the objectives. The data were analysed using descriptive statistics. Findings showed that the cost of Bt cotton production was slightly higher than that of conventional cotton. However, Bt cotton yielded a productivity increase of 0.81 t/ha. The cultivation of Bt cotton resulted in a higher net return (USD 2436/ha) compared to conventional cotton (USD 1624/ha). The results further indicated that the use of insecticides and pesticides in Bt cotton was significantly lower compared to traditional cotton, thereby contributing to the preservation of the natural environment. Overall, cultivation of Bt cotton is economically viable and may generate environmental benefits. Steps are warranted to disseminate and expand its cultivation.

RESUMEN

BACKGROUND: Advancement in agricultural biotechnology has resulted in increasing numbers of commercial varieties of genetically modified (GM) crops worldwide. Though several databases on GM crops are available, these databases generally focus on collecting and providing information on transgenic crops rather than on screening strategies. To overcome this, we constructed a novel tool named, Genetically Modified Organisms Identification Tool (GMOIT), designed to integrate basic and genetic information on genetic modification events and detection methods. RESULTS: At present, data for each element from 118 independent genetic modification events in soybean, maize, canola, and rice were included in the database. Particularly, GMOIT allows users to customize assay ranges and thus obtain the corresponding optimized screening strategies using common elements or specific locations as the detection targets with high flexibility. Using the 118 genetic modification events currently included in GMOIT as the range and algorithm selection results, a "6 + 4" protocol (six exogenous elements and four endogenous reference genes as the detection targets) covering 108 events for the four crops was established. Plasmids pGMOIT-1 and pGMOIT-2 were constructed as positive controls or calibrators in qualitative and quantitative transgene detection. CONCLUSIONS: Our study provides a simple, practical tool for selecting, detecting, and screening strategies for a sustainable and efficient application of genetic modification.

Asunto(s)

Productos Agrícolas , Glycine max , Oryza , Plantas Modificadas Genéticamente , Productos Agrícolas/genética , Plantas Modificadas Genéticamente/genética , Oryza/genética , Glycine max/genética , Zea mays/genética , Transgenes , Brassica napus/genética

RESUMEN

Genetically engineered (GE) cotton event MON 88702, producing Mpp51Aa2 (previously mCry51Aa2) from Bacillus thuringiensis (Bt), controls sucking pests, such as Lygus spp. (Hemiptera: Miridae) and thrips (Thysanoptera). Ingesting high doses of the insecticidal protein resulted in adverse effects on life table parameters of beneficial, predatory Orius spp. (Hemiptera: Anthocoridae). This triggered laboratory studies with more realistic food treatments, including different combinations of prey types with and without Bt protein to further characterize risks to this important group of non-target organisms. In this work, exclusive feeding of frozen spider mites (Tetranychus urticae, Acari: Tetranychidae) from Bt cotton confirmed adverse effects on longevity and fecundity of O. majusculus adults. Alternate feeding of Bt protein-containing spider mites and Bt-free Ephestia kuehniella (Lepidoptera: Pyralidae) eggs mitigated effects on longevity, but not on fecundity. When living larvae of Spodoptera littoralis (Lepidoptera: Noctuidae) from Bt cotton were fed to the predators, however, no effects on longevity and reproduction of female O. majusculus were observed, despite the fact that Bt protein concentrations in larvae were almost as high as concentrations in spider mites. When a diverse mix of prey species with various Bt protein concentrations is consumed in the field, it is unlikely that exposure of Orius spp. to Mpp51Aa2 is high enough to exert adverse effects on predator populations. MON 88702 cotton may thus be a valuable tool for integrated management of sucking pests.

Asunto(s)

Bacillus thuringiensis , Gossypium , Longevidad , Control Biológico de Vectores , Plantas Modificadas Genéticamente , Reproducción , Animales , Gossypium/genética , Gossypium/parasitología , Gossypium/crecimiento & desarrollo , Gossypium/metabolismo , Plantas Modificadas Genéticamente/genética , Plantas Modificadas Genéticamente/crecimiento & desarrollo , Plantas Modificadas Genéticamente/parasitología , Bacillus thuringiensis/genética , Reproducción/genética , Proteínas Bacterianas/genética , Proteínas Bacterianas/metabolismo , Conducta Predatoria , Fertilidad/genética , Spodoptera/crecimiento & desarrollo , Spodoptera/fisiología , Spodoptera/genética , Larva/crecimiento & desarrollo , Larva/genética , Toxinas de Bacillus thuringiensis/genética , Endotoxinas/genética , Endotoxinas/metabolismo , Heterópteros/genética , Heterópteros/fisiología , Heterópteros/crecimiento & desarrollo , Proteínas Hemolisinas/genética , Proteínas Hemolisinas/metabolismo , Tetranychidae/genética , Femenino

RESUMEN

Genetically modified crops (GMCs) have been discussed due to unknown safety, and thus, it is imperative to develop an effective detection technology. CRISPR/Cas is deemed a burgeoning technology for nucleic acid detection. Herein, we developed a novel detection method for the first time, which combined thermostable Cas12b with loop-mediated isothermal amplification (LAMP), to detect genetically modified (GM) soybeans in a customized one-pot vessel. In our method, LAMP-specific primers were used to amplify the cauliflower mosaic virus 35S promoter (CaMV35S) of the GM soybean samples. The corresponding amplicons activated the trans-cleavage activity of Cas12b, which resulted in the change of fluorescence intensity. The proposed bioassay was capable of detecting synthetic plasmid DNA samples down to 10 copies/µL, and as few as 0.05% transgenic contents could be detected in less than 40 min. This work presented an original detection method for GMCs, which performed rapid, on-site, and deployable detection.

Asunto(s)

Glycine max , Técnicas de Amplificación de Ácido Nucleico , Plantas Modificadas Genéticamente , Técnicas de Amplificación de Ácido Nucleico/métodos , Técnicas de Amplificación de Ácido Nucleico/instrumentación , Plantas Modificadas Genéticamente/genética , Plantas Modificadas Genéticamente/química , Glycine max/genética , Glycine max/química , Bioensayo/métodos , Sistemas CRISPR-Cas , Caulimovirus/genética , Proteínas Bacterianas/genética

RESUMEN

Bt soybean cultivation is increasing worldwide. The Cry1Ac protein expressed in Bt soybean efficiently controls several lepidopteran pests. The stink bug, Piezodorus guildinii (Westwood), a major pest for soybean in the Americas, is not controlled by Bt crops, although possible sub-lethal effects may occur. Even if there were no negative effects for sting bug, ingesting toxins could affect its bio-controllers. We tested through ELISA detection if P. guildinii ingests Cry1Ac from Bt soybean and possible effects on its development, reproduction, survival, and feeding behavior. Biological traits were evaluated under controlled conditions of nymphs and adults feeding on pods of near-isogenic cultivars DM5958iPRO (Bt) and DM59i (non-Bt). Feeding behavior was recorded using an AC-DC electropenetrography (EPG) device. Results indicated that P. guildinii ingested the Cry1Ac protein; however, nymphal period and accumulated survival percentage did not differ between cultivars. Feeding on Bt soybean pods did not affect fecundity (i.e., number of egg masses and eggs/female) nor egg viability. Different feeding behaviors were only detected on the pathway phase (stylet penetration into plant tissue), which was more pronounced in the Bt cultivar. However, the total duration of the feeding activities on seeds was numerically higher (ca. 2X) on Bt plants compared to non-Bt. This is the first study to demonstrate that P. guildinii does ingest the Cry1Ac protein and excrete it without being absorbed, probably explaining the lack of direct adverse effects on its biological parameters. EPG could indicate that Bt soybean plants might be less palatable than non-Bt to red-banded stink bug.

Asunto(s)

Glycine max , Heterópteros , Animales , Conducta Alimentaria , Reproducción , Semillas , Ninfa

RESUMEN

Glyphosate hormesis, identified as a potential means to enhance crop yields, encounters practical constraints because it is typically assessed through foliar applications. The expression and extend of hormesis in this approach are influenced by unpredictable environmental conditions, highlighting the need to explore alternative glyphosate application methods, such as seed treatment. This study aimed to assess glyphosate hormesis on growth rates and biomass accumulation in seedlings soybean cultivars. Two dose-response experiments [doses from 0 to 2880 g acid equivalent (ae) ha-1], one via foliar and one via seed, were conducted on three soybean cultivars [one non-glyphosate-resistant (NGR) and two glyphosate-resistant (GR, one RR and one RR2)]. In a subsequent experiment, three safe glyphosate doses (0, 90 and 180 g ae ha-1) applied via seed were evaluated on four soybean cultivars (two RR and two RR2). For foliar applications, the range of glyphosate doses increasing growth rates and dry biomass by 12-28 % were 5.6-45 g ae ha-1 for the NGR cultivar, of 45-720 g ae ha-1 for RR and of 11.25-180 g ae ha-1 for RR2. In the seed treatment, biomass increases of 16-60 % occurred at 45-180 g ae ha-1 for the NGR and RR cultivars, and 90-360 g ae ha-1 for RR2. Glyphosate doses of 90 and 180 g ae ha-1, applied via seeds, provided greater growth and biomass accumulation for the RR and RR2 soybean cultivars. Both foliar and seed applications of glyphosate increased growth and biomass accumulation in soybean cultivars, with seed treatments showing greater and more consistent enhancements. These findings propose practical and viable alternative for harnessing glyphosate hormesis to facilitate the early development of soybeans and potentially enhance crop yield.

Asunto(s)

Glifosato , Herbicidas , Glycine max , Plantones , Glicina/toxicidad , Hormesis , Herbicidas/toxicidad , Biomasa , Semillas

RESUMEN

Previous work demonstrated the utility of using human-derived intestinal epithelial cell (IEC) lines cultured as polarized monolayers on Transwell® filters to differentiate between hazardous and non-hazardous proteins. The current study seeks to further resolve appropriate concentrations for evaluating proteins of unknown hazard potential using the IEC experimental platform and leverages these parameters for evaluating the potential toxicity of insecticidal proteins characteristic of those expressed in genetically modified (GM) agricultural biotechnology crops. To establish optimal test protein concentrations, effects of several known hazardous (C. perfringens epsilon toxin, Listeriolysin O, Phaseolus vulgaris erythroagglutinin, E. coli Shiga toxin 1, C. difficile Toxin B and wheat germ agglutinin) and non-hazardous (Ara-h2, ß-lactoglobulin, fibronectin and Rubisco) proteins on IEC barrier integrity and cell viability were evaluated at concentration ranges. Two insecticidal proteins (AfIP-1A and AfIP-1B) were evaluated for effects in the IEC assay, a seven-day insecticidal bioassay, and assessed in a high-dose 14-day acute oral toxicity study in mice. The results obtained from the human in vitro IEC assay were consistent with results obtained from an in vivo acute oral toxicity study, both demonstrating that the combination of AfIP-1A and AfIP-1B do not exhibit any identifiable harmful impacts on mammalian cells.

Asunto(s)

Toxinas Bacterianas , Clostridioides difficile , Humanos , Animales , Ratones , Toxinas Bacterianas/metabolismo , Escherichia coli , Intestinos , Células Epiteliales , Mucosa Intestinal/metabolismo , Mamíferos

RESUMEN

Effects of Bacillus thuringiensis (Bt) cotton are at the forefront of an intense debate on the benefits of genetically modified (GM) crops among smallholder farmers in developing countries. Existing studies fail to control for confounders, selection bias, or cultivation bias from preferential treatment in the initial adoption phase. Addressing these concerns in this paper, I examine the impact of Bt cotton employing an unbalanced panel fixed-effects model of a crop yield and profit function on newly collected plot-level data in the most recent decade. Results show that Bt cotton yields have stagnated, have a null effect on profits, and have become more sensitive to pest pressure in the most recent decade. Though many studies have demonstrated higher crop yield and profit gains in the first decade of Bt cotton adoption that raised the average returns to the technology, the second decade shows convergence in benefits, which raises obvious questions about the prospect of GM technology. Since Bt cotton is the only GM crop technology widely adopted by smallholder farmers, the findings of this paper contribute to the broader public debate on the future of agricultural biotechnology.

RESUMEN

The co-expression of multiple antimicrobial peptides (AMPs) in genetically modified (GM) crops can give plants a broader antibacterial spectrum and lower the pathogen risk of drug resistance. Therefore, four penaeidins (shrimp-derived AMPs) were fused and encoded in an artificial gene (PEN1234), driven by the seed-specific promoter Pzein, with the aim of co-expression in seeds of transgenic rice. The resistant rice plants, acquired via Agrobacterium-mediated transformation and glufosinate screening, were identified by PCR and the modified disk-diffusion method, and eight GM lines with high AMP content in the seeds were obtained. Among them, the PenOs017 line had the largest penaeidin content, at approximately 251-300 µg/g in seeds and 15-47 µg/g in roots and leaves. The AMPs in the seeds kept their antibacterial properties even after the seed had been boiled in hot water and could significantly inhibit the growth of methicillin-resistant Staphylococcus aureus, and AMPs in the leaves could effectively inhibit Xanthomonas oryzae pv. Oryzae. The results indicate that PenOs017 seeds containing AMPs are an ideal raw-material candidate for antibiotic-free food and feed, and may require fewer petrochemical fungicides or bactericides for disease control during cultivation than conventional rice.

Asunto(s)

Staphylococcus aureus Resistente a Meticilina , Oryza , Plantas Modificadas Genéticamente/genética , Oryza/genética , Staphylococcus aureus Resistente a Meticilina/genética , Semillas/genética , Antibacterianos/farmacología

RESUMEN

Since the 1970s 'hybrid seeds' have been linked to many perceived perils of industrialized agriculture. This essay revisits the scholarship that helped produce a dominant critical assessment of hybrid seeds, situating its emergence in a series of events and interventions of the late twentieth century. It explores how the singular history of F1 hybrid corn inflected understandings of crop breeding and seed production in general, contributing to effective political mobilization against agroindustry as well as lasting confusion about the promises and pitfalls of distinct approaches to crop development and the nature of hybrid seeds.

RESUMEN

The initial compositional analysis of plants plays an important role within the internationally harmonized comparative safety assessment approach for genetically modified plants. Current EFSA guidance prescribes two types of comparison, namely difference tests with regard to a conventional comparator or control, and equivalence tests with regard to a collection of commercial reference varieties. The experience gained so far shows that most of the statistically significant differences between the test and control can be discounted based on the fact that they are still within equivalence limits of reference varieties with a presumed history of safe use. Inclusion of a test variety and reference varieties into field trial design, and of the statistical equivalence test would already suffice for the purpose of finding relevant parameters that warrant further assessment, hence both the inclusion of a conventional counterpart and the performance of difference testing can be omitted. This would also allow for the inclusion of safety testing regimes into plant variety testing VCU (value for cultivation and use) or other, independent variety trials.

Asunto(s)

Productos Agrícolas , Alimentos Modificados Genéticamente , Productos Agrícolas/genética , Plantas Modificadas Genéticamente/genética

RESUMEN

Genetically engineered (GE) cotton, MON 88702, is protected against certain sucking pests, such as plant bugs and thrips, by producing mCry51Aa2, a modified protein from Bacillus thuringiensis (Bt). Predatory pirate bugs (Orius spp.), natural enemies contributing to biological pest control, are also sensitive to the insecticidal protein when exposed continuously to high concentrations. We evaluated effects of MON 88702 on Orius majusculus when fed prey types with different mCry51Aa2 concentrations. When neonates were provided exclusively Tetranychus urticae spider mites reared on MON 88702 (high mCry51Aa2 content), adverse effects on predator survival and development were confirmed, compared with specimens fed prey from near-isogenic non-Bt cotton. When fed a mixture of T. urticae and Ephestia kuehniella eggs (mCry51Aa2-free), predator life table parameters were similar to the treatment where eggs were fed exclusively. When mCry51Aa2-containing spider mites were provided for a limited time at the beginning or the end of juvenile development, effects were less pronounced. While pirate bug nymphs showed similar consumption rates for prey from Bt and non-Bt cotton, choice experiments revealed a preference for E. kuehniella eggs over spider mites. Lepidopteran larvae (Spodoptera littoralis, high mCry51Aa2 content) or cotton aphids (Aphis gossypii, mCry51Aa2-free) reared on MON 88702 as alternative prey did not result in adverse effects on O. majusculus. Our study suggests limited risk of mCry51Aa2-producing cotton for O. majusculus, because its sensitivity for the Bt protein is relatively low and its natural food consists of diverse prey species with varying concentrations of Bt protein.

Asunto(s)

Bacillus thuringiensis , Heterópteros , Insecticidas , Mariposas Nocturnas , Animales , Proteínas Bacterianas/metabolismo , Larva , Mariposas Nocturnas/metabolismo , Insecticidas/farmacología , Bacillus thuringiensis/metabolismo , Conducta Predatoria

RESUMEN

Achieving the United Nations (UN) Sustainable Development Goals (SDGs) will require additional innovation investments and increased adoption of resulting technologies. This will be particularly important for improving food security, crop nutrient availability, and sustainability. This article presents some of the global costs of not adopting genetically modified (GM) crops and genome editing breeding.

Asunto(s)

Biotecnología , Fitomejoramiento , Plantas Modificadas Genéticamente/genética , Biotecnología/métodos , Agricultura/métodos , Edición Génica/métodos

RESUMEN

With the large-scale planting of genetically modified (GM) crops, consumers were more aware of biosafety. Onsite rapid diagnostic methods were advantageous to the regulation of GM products. In this study, a rapid, sensitive and portable detection method based on recombinase polymerase amplification were proposed based on RPA reaction and Cas12a cleavage reaction for GM ingredients, named RPA-Cas12a-GM. The results would be displayed by fluorescence signal (FS) and visual bands of lateral flow strip (LFS). RPA-Cas12a-GM method could be completed within 45 min, and the detection limit was as low as 45 copies/µL of the standard plasmid containing CP4-EPSPS gene and Cry1Ab/Ac gene. Furthermore, the detection coincidence rate of RPA-Cas12a-GM method was 100%. In conclusion, the proposed RPA-Cas12a-GM method based FS and LFS were sensitive, specific, rapid and visible for diagnosis of CP4-EPSPS gene and Cry1Ab/Ac gene without complex equipment, which provides technical support for the regulation of GM products in the field.

RESUMEN

Products for food and feed derived from genetically modified (GM) crops are only allowed on the market when they are deemed to be safe for human health and the environment. The European Food Safety Authority (EFSA) performs safety assessment including a comparative approach: the compositional characteristics of a GM genotype are compared to those of reference genotypes that have a history of safe use. Statistical equivalence tests are used to carry out such a comparative assessment. These tests are univariate and therefore only consider one measured variable at a time. Phenotypic data, however, often comprise measurements on multiple variables that must be integrated to arrive at a single decision on acceptance in the regulatory process. The surge of modern molecular phenotyping platforms further challenges this integration, due to the large number of characteristics measured on the plants. This paper presents a new multivariate equivalence test that naturally extends a recently proposed univariate equivalence test and allows to assess equivalence across all variables simultaneously. The proposed test is illustrated on plant compositional data from a field study on maize grain and on untargeted metabolomic data of potato tubers, while its performance is assessed on simulated data.

Asunto(s)

Alimentos Modificados Genéticamente , Humanos , Plantas Modificadas Genéticamente/genética , Inocuidad de los Alimentos , Productos Agrícolas/genética , Zea mays/genética