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1.
J Sports Sci ; 42(11): 1041-1049, 2024 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-39093053

RESUMEN

A person-centred approach was used to examine whether children with various actual and perceived motor competence (AMC and PMC) profiles differ in (enjoyment of) physical fitness (PF). The strength of the relationship between AMC and PMC was also assessed through aligned assessment tools. A sample of 287 7-11-year-old children (47.40% boys, Mage = 8.92 ± .78 years) was assessed on AMC with the KörperkoordinationsTest für Kinder (KTK3), and on PF with six validated fitness tests. Animated videos fully aligned with the AMC- and PF-tests were used to assess children's PMC and enjoyment of PF, respectively. Cluster analyses identified one convergent (i.e. high AMC-high PMC) and three partially convergent AMC-PMC profiles (i.e. low AMC-high PMC). Furthermore, children with relatively high AMC (i.e. high-average profile) reported higher PF (F = 30.99, p < .001), while children with relatively high PMC (i.e. average-high profile) reported higher enjoyment of PF (F = 9.02, p < .001). The correlation between AMC and PMC was significant but weak (r = .16). Overall, it seems important to invest in both children's AMC and PMC as they may support a higher (enjoyment of) PF, potentially leading to higher PA-levels.


When it comes to physical fitness, actual motor competence rather than perceived motor competence seemed to play the biggest role. As extra high perceived motor competence did not add additional benefits in terms of physical fitness, there might have been a ceiling effect of perceived motor competence.When looking at enjoyment of physical fitness, perceived motor competence seemed to be a more important factor than actual motor competence. However, profile analyses revealed that a relatively high perceived motor competence score may not fully compensate the loss of enjoyment in physical fitness when children's actual motor competence is relatively low.


Asunto(s)
Destreza Motora , Aptitud Física , Placer , Humanos , Masculino , Niño , Destreza Motora/fisiología , Aptitud Física/fisiología , Femenino , Prueba de Esfuerzo/métodos , Percepción/fisiología
2.
Health Sci Rep ; 7(9): e70020, 2024 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-39210996

RESUMEN

Background and Aims: In this study, a pediatric hospital was selected as the study setting, to investigate the effects of the environment of a pediatric operating room child-friendly space on preschool children under anesthesia. Methods: Interviews were conducted in pediatric hospitals through anesthesia of preschool children. This study randomizing children between the ages of 1 and 7 years (seven males, five females, mean age 3.8 years, standard deviation 0.7). Twelve participants took part in this interview, which was an in-depth interview of preschoolers' perceptions of anesthesia using generalized data analysis to assess young children's perceived preferences and sensitivity to the environment. Preschoolers' preferences for feeling under anesthesia are reflected in four main areas. Results: (1) Interesting and engaging environment. (2) Humanized design, free of fear and unfamiliarity. (3) Caring, patient, and compassionate care by healthcare professionals. (4) High cooperation of the child and good doctor-patient relationship. Conclusion: The key findings of this study may help designers and clinicians to design a toddler-friendly built environment where pediatric hospital child-friendly spaces are designed to benefit their health and maximize their cooperation and psychological recovery.

3.
Front Pharmacol ; 15: 1389953, 2024.
Artículo en Inglés | MEDLINE | ID: mdl-38828457

RESUMEN

Cardiovascular disease (CVD) is a serious public health risk, and prevention and treatment efforts are urgently needed. Effective preventive and therapeutic programs for cardiovascular disease are still lacking, as the causes of CVD are varied and may be the result of a multifactorial combination. Mitophagy is a form of cell-selective autophagy, and there is increasing evidence that mitophagy is involved in cardioprotective processes. Recently, many studies have shown that FUN14 domain-containing protein 1 (FUNDC1) levels and phosphorylation status are highly associated with many diseases, including heart disease. Here, we review the structure and functions of FUNDC1 and the path-ways of its mediated mitophagy, and show that mitophagy can be effectively activated by dephosphorylation of Ser13 and Tyr18 sites, phosphorylation of Ser17 site and ubiquitination of Lys119 site in FUNDC1. By effectively activating or inhibiting excessive mitophagy, the quality of mitochondria can be effectively controlled. The main reason is that, on the one hand, improper clearance of mitochondria and accumulation of damaged mitochondria are avoided, and on the other hand, excessive mitophagy causing apoptosis is avoided, both serving to protect the heart. In addition, we explore the possible mechanisms by which FUNDC1-mediated mitophagy is involved in exercise preconditioning (EP) for cardioprotection. Finally, we also point out unresolved issues in FUNDC1 and its mediated mitophagy and give directions where further research may be needed.

4.
ACS Synth Biol ; 13(4): 1382-1392, 2024 04 19.
Artículo en Inglés | MEDLINE | ID: mdl-38598783

RESUMEN

The functional analysis of protein nanopores is typically conducted in planar lipid bilayers or liposomes exploiting high-resolution but low-throughput electrical and optical read-outs. Yet, the reconstitution of protein nanopores in vitro still constitutes an empiric and low-throughput process. Addressing these limitations, nanopores can now be analyzed using the functional nanopore (FuN) screen exploiting genetically encoded fluorescent protein sensors that resolve distinct nanopore-dependent Ca2+ in- and efflux patterns across the inner membrane of Escherichia coli. With a primary proof-of-concept established for the S2168 holin, and thereof based recombinant nanopore assemblies, the question arises to what extent alternative nanopores can be analyzed with the FuN screen and to what extent alternative fluorescent protein sensors can be adapted. Focusing on self-assembling membrane peptides, three sets of 13 different nanopores are assessed for their capacity to form nanopores in the context of the FuN screen. Nanopores tested comprise both natural and computationally designed nanopores. Further, the FuN screen is extended to K+-specific fluorescent protein sensors and now provides a capacity to assess the specificity of a nanopore or ion channel. Finally, a comparison to high-resolution biophysical and electrophysiological studies in planar lipid bilayers provides an experimental benchmark for future studies.


Asunto(s)
Nanoporos , Membrana Dobles de Lípidos/metabolismo , Liposomas , Péptidos/metabolismo , Canales Iónicos
5.
BMC Public Health ; 24(1): 1019, 2024 Apr 12.
Artículo en Inglés | MEDLINE | ID: mdl-38609934

RESUMEN

BACKGROUND: Students' physical fitness, particularly aerobic fitness, has seriously declined during the COVID-19 epidemic. However, in the post-epidemic era, there are few studies on the methods of improving aerobic fitness. Understanding the dose-response relationship between physical activity and aerobic fitness is crucial for developing effective exercise prescriptions. METHOD: This retrospective study reviewed the Fun Running program at Wannan Medical College in China. We conducted a pre-post study design to analyze the impact of 15 weeks of Fun Running training on aerobic fitness. Middle and long-distance running pace (MLDR-P) was used as the primary indicator of aerobic fitness. A paired sample T-test was used to analyze the differences between the two MLDR-Ps. Pearson's correlation was used to examine the correlation between variables. Multiple linear regression was used to determine the extent to which Fun Running components explain the variance in MLDR-P. RESULTS: A total of 3244 college students participated in this study. 15 weeks of Fun Running training can significantly improve the MLDR-P in both females (P < 0.001, ES = 0.68) and males (P < 0.001, ES = 0.72). The MLDR-P was significantly correlated with Fun Running (R2 = 0.95, p < 0.05, for females; R2 = 0.96, p < 0.05, for males). The component that had the greatest impact on MLDR-P was pace (ß = 1.39, for females; ß = 1.09, for males), followed by distance (ß = 0.49, for females; ß = 0.15, for males), and last frequency (ß = -0.03, for all). CONCLUSION: This study fills the gap in research on the dose-response relationship between running and aerobic fitness among college students in the post-epidemic era. The results show that 15 weeks of Fun Running training can significantly improve aerobic fitness. Examination of the dose-response relationship between Fun Running and MLDR-P provides practitioners with valuable insights into prescribing aerobic fitness training, allowing them to develop more effective training programs. Future research should focus on how to implement a hierarchical Fun Running program effectively.


Asunto(s)
Ejercicio Físico , Carrera , Femenino , Masculino , Humanos , Estudios Retrospectivos , Terapia por Ejercicio , Aptitud Física
6.
Biomed Pharmacother ; 174: 116548, 2024 May.
Artículo en Inglés | MEDLINE | ID: mdl-38599064

RESUMEN

BACKGROUND: Various heart diseases ultimately lead to chronic heart failure (CHF). In CHF, the inflammatory response is associated with pyroptosis, which is mediated by the NOD-like receptor protein 3 (NLRP3) inflammasome. Fu Xin decoction (FXD) is commonly used in clinical practice to treat CHF and improve inflammatory conditions. However, the specific pharmacological mechanisms of action for FXD in these processes have yet to be fully understood. PURPOSE: The objective of this study was to examine the protective mechanism of FXT against CHF, both in H9c2 cells and mice. METHOD: A CHF mouse model was established, and the effect of FXD was observed via gavage. Cardiac function was evaluated using echocardiography, while serum BNP and LDH levels were analyzed to assess the severity of CHF. Hematoxylin and eosin staining (H&E) and Masson staining were performed to evaluate myocardial pathological changes, and TdT-mediated dUTP Nick-End Labeling staining was used to detect DNA damage. Additionally, doxorubicin was utilized to induce myocardial cell injury in H9c2 cells, establishing a relevant model. CCK8 was used to observe cell viability and detect LDH levels in the cell supernatant. Subsequently, the expression of pyroptosis-related proteins was detected using immunohistochemistry, immunofluorescence, and western blotting. Finally, the pharmacological mechanism of FXD against CHF was further validated by treating H9c2 cells with an NLRP3 activator and inducing NLRP3 overexpression. RESULT: According to current research findings, echocardiography demonstrated a significant improvement of cardiac function by FXD, accompanied by reduced levels of BNP and LDH, indicating the amelioration of cardiac injury in CHF mice. FXD exhibited the ability to diminish serum CRP and MCP inflammatory markers in CHF mice. The results of HE and Masson staining analyses revealed a significant reduction in pathological damage of the heart tissue following FXD treatment. The CCK8 assay demonstrated the ability of FXD to enhance H9c2 cell viability, improve cell morphology, decrease LDH levels in the cell supernatant, and alleviate cell damage. Immunohistochemistry, Western blotting, and immunofluorescence staining substantiated the inhibitory effect of FXD on the NLRP3/caspase-1/GSDMD pyroptosis signaling pathway in both CHF and H9c2 cell injury models. Ultimately, the administration of the NLRP3 activator (Nigericin) and the overexpression of NLRP3 counteract the effects of FXD on cardiac protection and pyroptosis inhibition in vitro. CONCLUSION: FXD exhibits a cardioprotective effect, improving CHF and alleviating pyroptosis by inhibiting the NLRP3/caspase-1/GSDMD pathway.


Asunto(s)
Medicamentos Herbarios Chinos , Insuficiencia Cardíaca , Proteína con Dominio Pirina 3 de la Familia NLR , Piroptosis , Animales , Ratones , Caspasa 1/efectos de los fármacos , Caspasa 1/metabolismo , Línea Celular , Enfermedad Crónica , Modelos Animales de Enfermedad , Medicamentos Herbarios Chinos/farmacología , Gasderminas/efectos de los fármacos , Gasderminas/metabolismo , Insuficiencia Cardíaca/tratamiento farmacológico , Insuficiencia Cardíaca/metabolismo , Inflamasomas/metabolismo , Inflamasomas/efectos de los fármacos , Péptidos y Proteínas de Señalización Intracelular/metabolismo , Ratones Endogámicos C57BL , Miocitos Cardíacos/efectos de los fármacos , Miocitos Cardíacos/metabolismo , Miocitos Cardíacos/patología , Proteína con Dominio Pirina 3 de la Familia NLR/efectos de los fármacos , Proteína con Dominio Pirina 3 de la Familia NLR/metabolismo , Proteínas de Unión a Fosfato/metabolismo , Piroptosis/efectos de los fármacos , Transducción de Señal/efectos de los fármacos
7.
J Youth Adolesc ; 53(2): 386-396, 2024 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-37775691

RESUMEN

There is a critical gap in our understanding of how peer relationships contribute to positive youth development. To address this gap the current study uses longitudinal social network data to examine if fun youth were socially desirable, inclusive of peers, and positive agents of social influence during the transition to adolescence. Participants were 210 students (47% female; Mage = 11.55 years at the outset) from 8 classes in 3 schools in a small Lithuanian city. Each child received a fun score consisting of nominations from classmates as "someone who is fun to be around". Participants also nominated up to five classmates as friends. Fun students received more friendship nominations than their peers and they maintained higher levels of social desirability over time. Fun youth appeared to be inclusive of their peers in that fun youth did not appear to have preferences to befriend only those like themselves. The friends of fun youth were positively influenced such that they were perceived to be more fun over time. The findings suggests that fun youth may promote positive social change within peer groups.


Asunto(s)
Conducta del Adolescente , Amigos , Niño , Humanos , Adolescente , Femenino , Masculino , Grupo Paritario , Instituciones Académicas , Dinámica de Grupo
8.
China Pharmacy ; (12): 1226-1231, 2024.
Artículo en Chino | WPRIM (Pacífico Occidental) | ID: wpr-1030849

RESUMEN

OBJECTIVE To investigate the effects of vorinostat (SAHA) on immune function in rats with Sjögren syndrome (SS) by regulating interleukin-6(IL-6)/signal transducer and activator of transcription 3 (STAT3). METHODS SS model was constructed by complete/incomplete Freund’s adjuvant immune induction, and the rats with successful modeling were divided into model group, SAHA low-dose, medium-dose and high-dose groups (25, 50, 100 mg/kg), and high-dose SAHA+IL-6/STAT3 signaling pathway activator group (100 mg/kg SAHA+0.05 mg/kg recombinant IL-6 protein), with 10 rats in each group. Another 10 healthy rats were collected as the control group. Rats in each group were intraperitoneally injected with the corresponding drug/ normal saline, once a day, for consecutive 2 weeks. The water intake (1 d) and saliva flow (10 min) of rats in each group were measured, and the spleen index and submandibular gland index were measured; pathological changes in submandibular gland tissue were observed. The serum levels of interferon-γ (IFN-γ), IL-17 and IL-10 were detected, and the differentiation of T lymphocyte subgroups in peripheral blood was also detected; the expressions of IL-6/STAT3 signaling pathway-related proteins in submandibular gland were detected. RESULTS Compared with the control group, obvious focal infiltration of lymphocytes was observed in the submandibular gland tissue of rats in the model group, and the number of acinar cells was decreased significantly; the water intake, spleen index, pathological score of submandibular gland, serum levels of IFN-γ and IL-17, and the ratios of helper T cell 1 (Th1) and Th17 as well as the protein expression of IL-6 and the protein phosphorylation of STAT3 in submandibular gland were increased obviously (P<0.05); the saliva flow, submandibular gland index, serum level of IL-10 and regulatory T cell (Treg) ratio were obviously decreased (P<0.05). Compared with the model group, the damage to the submandibular gland in SAHA groups was relieved, the quantitative indexes were obviously improved in dose-dependent manner (P<0.05); recombinant IL-6 protein could reverse the improvement effect of SAHA on above indexes of rats (P<0.05). CONCLUSIONS SAHA can relieve the injury of submandibular gland and regulate its immune function in SS rats, which may be related to inhibiting the IL-6/STAT3 signaling pathway.

9.
J Pers ; 2023 Nov 28.
Artículo en Inglés | MEDLINE | ID: mdl-38014722

RESUMEN

OBJECTIVE: Children perceived by peers as someone who is fun reap interpersonal rewards, but little is known about what makes someone fun or how being fun leads to social success. The present study is designed to identify what qualities makes someone fun and how being fun leads to social success. METHOD: Two studies of children in primary and middle school are reported. Participants in the present investigation attended a public-school representative of Florida school children in terms of ethnicity and income. In the first study, 351 (179 girls, 172 boys) students (8-11 years old) completed surveys twice (M = 8.5 weeks apart) during an academic year, describing the qualities of "someone who is fun." RESULTS: At both time points, kindness and humor were rated as more important than buffoonery. In the second study, 394 (210 girls, 184 boys) students (8-13 years old) completed peer nomination surveys thrice (M = 8.5 weeks apart) during an academic year. Replicating previous findings, being fun predicted increases in social status (i.e., likeability and popularity). CONCLUSIONS: Unique to this study, full longitudinal mediation analyses indicated that being perceived as fun early in the school year predicted friend gain from the beginning to the middle of the school year, which, in turn, predicted increases in perceived likeability and popularity from the middle to the end of the school year. The findings were unique to being fun. Kindness and humor did not predict friend gain.

10.
Neuroscience ; 530: 1-16, 2023 Oct 15.
Artículo en Inglés | MEDLINE | ID: mdl-37625686

RESUMEN

Mitophagy plays a significant role in modulating the activation of pyrin domain-containing protein 3 (NLRP3) inflammasome, which is a major contributor to the inflammatory response that exacerbates cerebral ischemia-reperfusion (I/R) injury. Despite this, the transcriptional regulation mechanism that governs mitophagy remains unclear. This study sought to explore the potential mechanism of Forkhead Box P1 (Foxp1) and its impact on cerebral I/R injury. We investigated the potential neuroprotective role of Foxp1 in cerebral I/R injury by the middle cerebral artery occlusion (MCAO) mouse model. Additionally, we assessed whether FUN14 domain-containing protein 1 (FUNDC1) could rescue the protective effect of Foxp1. Our results showed that overexpression of Foxp1 prevented brain damage during cerebral I/R injury and promoted NLRP3 inflammasome activation, whereas knockdown of Foxp1 had the opposite effect. Notably, Foxp1 overexpression directly promotes FUNDC1 expression, enhanced mitophagy activation, and inhibited the inflammatory response mediated by the NLRP3 inflammasome. Furthermore, we confirmed through chromatin immunoprecipitation (ChIP) and luciferase reporter assays that FUNDC1 is a direct target gene of Foxp1 downstream. Furthermore, the knockdown of FUNDC1 reversed the increased activation of mitophagy and suppressed NLRP3 inflammasome activation induced by Foxp1 overexpression. Collectively, our findings suggest that Foxp1 inhibits NLRP3 inflammasome activation through FUNDC1 to reduce cerebral I/R injury.

11.
Child Adolesc Psychiatry Ment Health ; 17(1): 93, 2023 Aug 03.
Artículo en Inglés | MEDLINE | ID: mdl-37537603

RESUMEN

BACKGROUND: Research on school maladjustment has increasingly focused on social skills, such as the ability to control emotions, collaborate with others, and achieve goals. Social and emotional learning (SEL) is one approach to nurturing social skills. However, few preventive interventions to promote SEL have been conducted among young children, particularly in Asian countries, including Japan. Therefore, this study examined the effectiveness of an SEL program-Fun FRIENDS-among children in Japan. METHODS: In mid-2022, the Fun FRIENDS program was administered to 115 children aged 4-5 years, who were enrolled in two kindergartens. The program was administered to the entire class as part of their kindergarten activities. The control group included 93 children in three kindergartens. This study included 94 participants (81.7%) in the intervention group and 66 (71.0%) in the control group, whose parents agreed with the assessment of their skills. Fun FRIENDS is a support program based on a cognitive-behavioral approach. The program aims to teach children how to cope with anxiety and stress and develop resilience and confidence to face difficulties. The program includes 10 sessions, each lasting approximately 1 h and conducted once per week. To examine the program's effectiveness, teachers evaluated these children's social skills before and after program implementation using the Social Skill Scale. RESULTS: Results showed significant post-intervention improvements in self-control and cooperation scores among children in the intervention group, compared with pre-intervention. Further, post-intervention self-control and cooperation scores were significantly higher among children in the intervention group than the control group. CONCLUSIONS: SEL implemented on a class-wide basis could be effective in early childhood. An early approach targeting preschool-aged children is necessary to prevent school maladjustment. A universal approach implemented on a whole-class basis could contribute to improving children's social skills.

12.
Burns Trauma ; 11: tkad018, 2023.
Artículo en Inglés | MEDLINE | ID: mdl-37274155

RESUMEN

Cerebral ischemia is a neurological disorder associated with complex pathological mechanisms, including autophagic degradation of neuronal mitochondria, or termed mitophagy, following ischemic events. Despite being well-documented, the cellular and molecular mechanisms underlying the regulation of neuronal mitophagy remain unknown. So far, the evidence suggests neuronal autophagy and mitophagy are separately regulated in ischemic neurons, the latter being more likely activated by reperfusional injury. Specifically, given the polarized morphology of neurons, mitophagy is regulated by different neuronal compartments, with axonal mitochondria being degraded by autophagy in the cell body following ischemia-reperfusion insult. A variety of molecules have been associated with neuronal adaptation to ischemia, including PTEN-induced kinase 1, Parkin, BCL2 and adenovirus E1B 19-kDa-interacting protein 3 (Bnip3), Bnip3-like (Bnip3l) and FUN14 domain-containing 1. Moreover, it is still controversial whether mitophagy protects against or instead aggravates ischemic brain injury. Here, we review recent studies on this topic and provide an updated overview of the role and regulation of mitophagy during ischemic events.

13.
Mol Biol Rep ; 50(8): 6557-6568, 2023 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-37338733

RESUMEN

BACKGROUND: Chronic kidney disease (CKD) is a serious health threat worldwide. Defective mitophagy has been reported to induce mitochondrial dysfunction, which is closely associated with CKD pathogenesis. Honokiol (HKL) is a bioactive component of Magnolia officinalis that has multiple efficacies. Our study aimed to investigate the effect of HKL on a CKD rat model and explore the possible mechanisms of mitophagy mediated by Bcl-2 interacting protein 3 and BNIP3-like (NIX) (also known as the BNIP3/NIX pathway) and FUN14 domain-containing 1 (the FUNDC1 pathway) and the role of the AMP-activated protein kinase (AMPK) pathway. METHODS: A CKD rat model was established by feeding the animals dietary adenine (0.75% w/w, 3 weeks). Simultaneously, the treatment group was given HKL (5 mg/kg/day, 4 weeks) by gavage. Renal function was assessed by measuring serum creatinine (Scr) and blood urea nitrogen (BUN) levels. Pathological changes were analyzed by periodic acid-Schiff (PAS) and Masson's trichrome staining. Protein expression was evaluated by Western blotting and immunohistochemistry. RESULTS: HKL treatment ameliorated the decline in renal function and reduced tubular lesions and interstitial fibrosis in CKD rats. Accordingly, the renal fibrosis markers Col-IV and α-SMA were decreased by HKL. Moreover, HKL suppressed the upregulation of the proapoptotic proteins Bad and Bax and Cleaved caspase-3 expression in CKD rats. Furthermore, HKL suppressed BNIP3, NIX and FUNDC1 expression, leading to the reduction of excessive mitophagy in CKD rats. Additionally, AMPK was activated by adenine, and HKL reversed this change and significantly decreased the level of activated AMPK (phosphorylated AMPK, P-AMPK). CONCLUSION: HKL exerted a renoprotective effect on CKD rats, which was possibly associated with BNIP3/NIX and FUNDC1-mediated mitophagy and the AMPK pathway.


Asunto(s)
Mitofagia , Insuficiencia Renal Crónica , Ratas , Animales , Proteínas Quinasas Activadas por AMP/metabolismo , Mitocondrias/metabolismo , Insuficiencia Renal Crónica/tratamiento farmacológico , Insuficiencia Renal Crónica/metabolismo , Proteínas Mitocondriales/metabolismo , Proteínas de la Membrana/metabolismo
14.
Environ Sci Pollut Res Int ; 30(23): 63603-63619, 2023 May.
Artículo en Inglés | MEDLINE | ID: mdl-37046168

RESUMEN

In the Xishan coalfield of northern China, the stratified stacking of soil and gangue was applied to limit the acid pollution from high-sulfur coal gangue. In this study, we found that stratified stacking can effectively neutralize the acidity, with the pH value of gangue-leaching water being 6.02-8.13. In contrast to the acidic contaminated area, most of the microorganisms in the study area sediment were neutrophilic, with the main genera being Arthrobacter, Pseudorhodobacter, Pseudomonas, and Rhodoferax. A variety of iron- and sulfur-metabolizing bacteria was discovered in the gangue-leaching sediment, with the total relative abundance ranging from 4.20 to 23.75%, of which the iron-reducing bacteria (FeRB) accounted for the highest percentage. The distributions of these functional microorganisms in the samples were significantly influenced by Fe and S. The co-occurrence network analysis revealed a significant positive correlation between the iron- and sulfur-metabolizing bacteria in the sediment (93.75%), indicating a strong reciprocal symbiotic relationship between these bacteria. The iron and sulfur metabolism genes in the sediment were predicted and compared based on the Tax4Fun functional prediction method. Results showed that functional genes related to iron metabolism were highly expressed in the gangue-leaching sediment. This study enhances the understanding of iron and sulfur metabolism in gangue-leaching contaminated areas.


Asunto(s)
Carbón Mineral , Hierro , Hierro/análisis , Carbón Mineral/análisis , Contaminación Ambiental/análisis , Suelo/química , Azufre/análisis , China
15.
Molecules ; 28(6)2023 Mar 16.
Artículo en Inglés | MEDLINE | ID: mdl-36985654

RESUMEN

Polysaccharides from the species of Boletaceae (Boletales, Agaricomycetes, Basidiomycota) are economically significant to both functional foods and medicinal industries. The crude polysaccharide from Butyriboletus pseudospeciosus (BPP) was prepared, and its physicochemical properties were characterized through the use of consecutive experimental apparatus, and its impact on the gut microbiota of Kunming mice was evaluated. Analyses of the structure characteristics revealed that BPP was mainly composed of Man, Glc, and Gal, possessing the pyranose ring and ß/α-glycosidic linkages. TG analysis exhibited that BPP had great heat stability. The SEM observation performed demonstrated that BPP appeared with a rough, dense, and porous shape. Through the BPP intervention, the serum and fecal biochemical index in mice can be improved obviously (p < 0.05). The abundance of beneficial microbiota in the BPP-treated group was significantly increased, while the abundance of harmful microbiota was significantly decreased (p < 0.05). Based on the Tax4Fun, we also revealed the relationship between the species of gut microbiota and showed that the high dose of BPP has significantly changed the functional diversities compared with those in other groups (p < 0.05). The results suggest that B. pseudospeciosus could serve as potential functional food or medicine.


Asunto(s)
Ascomicetos , Basidiomycota , Microbioma Gastrointestinal , Ratones , Animales , Polisacáridos/química , Basidiomycota/química , Cuerpos Fructíferos de los Hongos/química
16.
Heliyon ; 9(3): e14597, 2023 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-36994408

RESUMEN

Digital platforms and tech giants have led to a rapid shift in values and traditional ways of working. Although "diligence" has long been essential for work success and promotion, employees in modern companies are reluctant to blindly follow this attitude. Many well-known Western companies, such as Facebook and Google, see fun in the workplace as conducive to productivity and creative behavior. We investigated the associations of fun at work with experienced fun, employees' creative behavior, managers' support for fun, and trust in a Chinese context using different scales. Discriminant validity was confirmed by confirmatory factor analysis. A total of 508 workers from Taiwan and mainland China participated in the study and completed questionnaires. A key finding was that fun at work was positively related to employees' creative behavior. In addition, moderators of managerial support for fun and trust between the workplace and experienced fun were confirmed. These results can serve as a reference for Chinese managers who want to encourage creative behaviors and prevent negative behaviors in the workplace. In practice, results suggest that fun should be allowed more in the workplace because it could contribute to positive outcomes. However, managers should create a workplace that is fun, allows for creativity, and at the same time leads to high productivity.

17.
Acta Biochim Biophys Sin (Shanghai) ; 55(3): 508-517, 2023 Mar 25.
Artículo en Inglés | MEDLINE | ID: mdl-36896644

RESUMEN

Candida albicans ( C. albicans) is an opportunistic pathogen in humans and possesses a white-opaque heritable switching system. Wor1 is a master regulator of white-opaque switching and is essential for opaque cell formation in C. albicans. However, the regulatory network of Wor1 in white-opaque switching is still vague. In this study, we obtain a series of Wor1-interacting proteins using LexA-Wor1 as bait. Among these proteins, function unknown now 30 (Fun30) interacts with Wor1 in vitro and in vivo. Fun30 expression is upregulated in opaque cells at the transcriptional and protein levels. Loss of FUN30 attenuates white-to-opaque switching, while ectopic expression of FUN30 significantly increases white-to-opaque switching in an ATPase activity-dependent manner. Furthermore, FUN30 upregulation is dependent on CO 2; loss of FLO8, a key CO 2-sensing transcriptional regulator, abolishes FUN30 upregulation. Interestingly, deletion of FUN30 affects the WOR1 expression regulation feedback loop. Thus, our results indicate that the chromatin remodeller Fun30 interacts with Wor1 and is required for WOR1 expression and opaque cell formation.


Asunto(s)
Candida albicans , Proteínas Fúngicas , Humanos , Candida albicans/metabolismo , Proteínas Fúngicas/genética , Proteínas Fúngicas/metabolismo , Nucleosomas/metabolismo , Factores de Transcripción/genética , Factores de Transcripción/metabolismo , Fenotipo
18.
Bioresour Technol ; 372: 128699, 2023 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-36731617

RESUMEN

The performance, microbial community and functional genes of a sequencing batch biofilm reactor (SBBR) were investigated in treating mariculture wastewater under different aerobic/anoxic alternation number. The removal efficiency of chemical oxygen demand (COD) and NH4+-N kept at 95.66 ± 1.83 % and 90.28 ± 2.42 % under aerobic/anoxic alternation number between 1 and 4. The total nitrogen (TN) removal efficiency gradually decreased from 94.45 ± 1.12 % to 83.06 ± 1.25 % with the increase of aerobic/anoxic alternative number from 1 to 4. The nitrification rates and their corresponding enzymatic activities increased slightly with the increase of aerobic/anoxic alternation number, whereas the denitrifying process had the contrary results. The variation of aerobic/anoxic alternation number obviously affected the microbial diversity and abundance. The microbial network structure and keystone taxa were different under different aerobic/anoxic alternation number. The functional genes abundance for the denitrification pathway decreased with the increase of aerobic/anoxic alternation number.


Asunto(s)
Eliminación de Residuos Líquidos , Aguas Residuales , Eliminación de Residuos Líquidos/métodos , Reactores Biológicos , Nitrificación , Consorcios Microbianos , Biopelículas , Nitrógeno/metabolismo , Desnitrificación
19.
Chin J Integr Med ; 29(1): 81-88, 2023 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-34731432

RESUMEN

Mitophagy is one of the important targets for the prevention and treatment of myocardial ischemia/reperfusion injury (MIRI). Moderate mitophagy can remove damaged mitochondria, inhibit excessive reactive oxygen species accumulation, and protect mitochondria from damage. However, excessive enhancement of mitophagy greatly reduces adenosine triphosphate production and energy supply for cell survival, and aggravates cell death. How dysfunctional mitochondria are selectively recognized and engulfed is related to the interaction of adaptors on the mitochondrial membrane, which mainly include phosphatase and tensin homolog deleted on chromosome ten (PTEN)-induced kinase 1/Parkin, hypoxia-inducible factor-1 α/Bcl-2 and adenovirus e1b19k Da interacting protein 3, FUN-14 domain containing protein 1 receptor-mediated mitophagy pathway and so on. In this review, the authors briefly summarize the main pathways currently studied on mitophagy and the relationship between mitophagy and MIRI, and incorporate and analyze research data on prevention and treatment of MIRI with Chinese medicine, thereby provide relevant theoretical basis and treatment ideas for clinical prevention of MIRI.


Asunto(s)
Mitofagia , Daño por Reperfusión Miocárdica , Humanos , Mitocondrias/metabolismo , Mitofagia/genética , Proteínas Quinasas/genética , Proteínas Quinasas/metabolismo
20.
Artículo en Inglés | WPRIM (Pacífico Occidental) | ID: wpr-971316

RESUMEN

Mitophagy is one of the important targets for the prevention and treatment of myocardial ischemia/reperfusion injury (MIRI). Moderate mitophagy can remove damaged mitochondria, inhibit excessive reactive oxygen species accumulation, and protect mitochondria from damage. However, excessive enhancement of mitophagy greatly reduces adenosine triphosphate production and energy supply for cell survival, and aggravates cell death. How dysfunctional mitochondria are selectively recognized and engulfed is related to the interaction of adaptors on the mitochondrial membrane, which mainly include phosphatase and tensin homolog deleted on chromosome ten (PTEN)-induced kinase 1/Parkin, hypoxia-inducible factor-1 α/Bcl-2 and adenovirus e1b19k Da interacting protein 3, FUN-14 domain containing protein 1 receptor-mediated mitophagy pathway and so on. In this review, the authors briefly summarize the main pathways currently studied on mitophagy and the relationship between mitophagy and MIRI, and incorporate and analyze research data on prevention and treatment of MIRI with Chinese medicine, thereby provide relevant theoretical basis and treatment ideas for clinical prevention of MIRI.


Asunto(s)
Humanos , Mitocondrias/metabolismo , Mitofagia/genética , Daño por Reperfusión Miocárdica , Proteínas Quinasas/metabolismo
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