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Lake ice phenology (LIP), hiding information about lake energy and material exchange, serves as an important indicator of climate change. Utilizing an efficient technique to swiftly extract lake ice information is crucial in the field of lake ice research. The Bayesian ensemble change detection (BECD) algorithm stands out as a powerful tool, requiring no threshold compared to other algorithms and, instead, utilizing the probability of abrupt changes to detect positions. This method is predominantly employed by automatically extracting change points from time series data, showcasing its efficiency and accuracy, especially in revealing phenological and seasonal characteristics. This paper focuses on Bosten Lake (BL) and employs PMRS data in conjunction with the Bayesian change detection algorithm. It introduces an automated method for extracting LIP information based on the Bayesian change detection algorithm. In this study, the BECD algorithm was employed to extract lake ice phenology information from passive microwave remote sensing data on Bosten Lake. The reliability of the passive microwave remote sensing data was further investigated through cross-validation with MOD10A1 data. Additionally, the Mann-Kendall non-parametric test was applied to analyze the trends in lake ice phenology changes in Bosten Lake. Spatial variations were examined using MOD09GQ data. The results indicate: (1) The Bayesian change detection algorithm (BCDA), in conjunction with PMRS data, offers a high level of accuracy and reliability in extracting the lake ice freezing and thawing processes. It accurately captures the phenological parameters of BL's ice. (2) The average start date of lake ice freezing is in mid-December, lasting for about three months, and the start date of ice thawing is usually in mid-March. The freezing duration (FD) of lake ice is relatively short, shortening each year, while the thawing speed is faster. The stability of the lake ice complete ice cover duration is poor, averaging 84 days. (3) The dynamic evolution of BL ice is rapid and regionally distinct, with the lake center, southwest, and southeast regions being the earliest areas for ice formation and thawing, while the northwest coastal and Huang Shui Gou areas experience later ice formation. (4) Since 1978, BL's ice has exhibited noticeable trends: the onset of freezing, the commencement of thawing, complete thawing, and full freezing have progressively advanced in regard to dates. The periods of full ice coverage, ice presence, thawing, and freezing have all shown a tendency toward shorter durations. This study introduces an innovative method for LIP extraction, opening up new prospects for the study of lake ecosystem and strategy formulation, which is worthy of further exploration and application in other lakes and regions.

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Materials made from coal gangue (CGEr) can be used for ecological restoration in mining areas. This paper comprehensively analyzed the influence of the freeze-thaw process on the performance of CGEr and the environmental risk of heavy metals. The safety of CGEr was assessed by sediment quality guidelines (SQGs), geological accumulation index (Igeo), potential ecological risk index (RI), and risk assessment code (RAC). The freeze-thaw process reduced the performance of CGEr, that the water retention of CGEr decreased from 1.07 (g water/g soil) to 0.78 (g water/g soil), and the loss rate of soil and water increased from 1.07 to 4.30%. The freeze-thaw process reduced the ecological risk of CGEr, the Igeo of Cd and Zn decreased from 1.14 to 0.13 and 0.53 to 0.3, respectively, and the RI of Cd decreased by 50% from 0.297 to 0.147. Reaction experiments and correlation analysis showed that the freeze-thaw process destroyed the pore structure of the material, resulting in the degradation of its properties. Water molecules undergo phase transformation during freeze-thaw, and particles were squeezed by ice crystals to form agglomerates. The formation of granular aggregates resulted in the enrichment of heavy metals in the aggregates. Influenced by the freeze-thaw process, specific functional groups such as -OH were more exposed on the surface of the material, which affected the occurrence form of heavy metals and thus reduced the potential ecological risk of the material. This study provides an important basis for the better application of ecological restoration materials of CGEr.

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The individual coupling processes of two-phase materials are controlled to some extent by damage theory. However, the existing theory is not sufficient to explain the effect of pore pressure on mortar materials under freeze-thaw action. In order to predict the resistance of saturated mortars during rapid cooling and to describe the physical behavior of the pore structure, the authors derived in detail the governing equations of saturated mortars during freezing in the framework of the pore elasticity theory and analyzed the sensitivity of physical parameters to the influence of temperature stresses by means of stress-strain calculations. In addition, the effects of phase change and latent heat of freezing on the local thermodynamic equilibrium are considered, and a mathematical model is established for quantitatively simulating the temperature distribution of the specimen. This model is reformulated and extended in the current work to intuitively reveal the effect of concrete dimensions on the temperature hysteresis effect. The results of the numerical model calculations show that during the freezing process, for the specimen with dimensions of 50 mm × 50 mm × 50 mm and a water-cement ratio of 0.6, the maximum temperature difference from center to surface is 10 °C, the maximum vertical strain on the surface is 4.27 × 10-4, and the maximum pore water pressure at the center of the specimen is 76 MPa. The model calculation results present a similar pattern to the physical interpretation and reference results, thus effectively evaluating the freezing damage process of saturated mortar.

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The aim of this study is to prepare a dual layer polyvinyl (PVA) patch using a combination of electrospinning techniques and cryogelation (freeze-thaw process) then subsequently to investigate the effect of freeze-thaw cycles, nanofiber thickness, and diclofenac sodium (DS) loading on the physicochemical and mechanical properties and formulation of dual layer PVA patches composed of electrospun PVA nanofibers and PVA cryogel. After the successful preparation of the dual layer PVA patch, the prepared patch was subjected to investigation to assess the effect of freeze-thaw cycles, nanofiber thickness and percentages of DS loading on the morphology, physiochemical and mechanical properties. Various spectroscopic techniques such as scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transform infrared (FTIR), water contact angle, and tensile tests were used to evaluate the physicochemical and mechanical properties of prepared dual layer PVA patches. The morphological structures of the dual layer PVA patch demonstrated the effectiveness of both techniques. The effect of freeze-thaw cycles, nanofiber thickness, and DS percentage loading on the crystallinity of a dual layer PVA patch was investigated using XRD analysis. The presence of a distinct DS peak in the FTIR spectrum indicates the compatibility of DS in a dual layer PVA patch through in-situ loading. All prepared patches were considered highly hydrophilic because the data obtained was less than 90°. The increasing saturation of DS within the PVA matrix increases the tensile strength of prepared patches, however decreased its elasticity. Evidently, the increasing of electrospun PVA nanofibers thickness, freeze-thaw cycles, and the DS saturation has improved the physicochemical and mechanical properties of the DS medicated dual layer PVA patches, making them a promising biomaterial for transdermal drug delivery applications.

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Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) is a complex neuroimmune disorder characterized by numerous symptoms of unknown etiology. The ME/CFS immune markers reported so far have failed to generate a clinical consensus, perhaps partly due to the limitations of biospecimen biobanking. To address this issue, we performed a comparative analysis of the impact of long-term biobanking on previously identified immune markers and also explored additional potential immune markers linked to infection in ME/CFS. A correlation analysis of marker cryostability across immune cell subsets based on flow cytometry immunophenotyping of fresh blood and frozen PBMC samples collected from individuals with ME/CFS (n = 18) and matched healthy controls (n = 18) was performed. The functionality of biobanked samples was assessed on the basis of cytokine production assay after stimulation of frozen PBMCs. T cell markers defining Treg subsets and the expression of surface glycoprotein CD56 in T cells and the frequency of the effector CD8 T cells, together with CD57 expression in NK cells, appeared unaltered by biobanking. By contrast, NK cell markers CD25 and CD69 were notably increased, and NKp46 expression markedly reduced, by long-term cryopreservation and thawing. Further exploration of Treg and NK cell subsets failed to identify significant differences between ME/CFS patients and healthy controls in terms of biobanked PBMCs. Our findings show that some of the previously identified immune markers in T and NK cell subsets become unstable after cell biobanking, thus limiting their use in further immunophenotyping studies for ME/CFS. These data are potentially relevant for future multisite intervention studies and cooperative projects for biomarker discovery using ME/CFS biobanked samples. Further studies are needed to develop novel tools for the assessment of biomarker stability in cryopreserved immune cells from people with ME/CFS.

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Retrospective studies involving the screening of frozen stored collections of samples are commonplace when a new threat emerges, but it has been demonstrated that the freeze-thaw process can affect bacterial viability. The study of colistin-resistant bacteria in human and animal samples is an example of this issue. In this study, we compared culture-based and PCR-based methods for analyzing relative occurrence and diversity of colistin-resistant bacteria in caecal samples to determine the most appropriate method for frozen samples. Thus, 272 samples from the caecal contents of healthy pigs were tested before and after a 6-month freezing period. A selective medium was used when traditional isolation of colistin-resistant bacteria was tested, while a real-time SYBR® Green I PCR assay was applied for mcr-1 quantification. The number of samples with colistin-resistant isolates was higher in fresh samples (247/272) than in frozen ones (67/272) and showed a higher diversity of colistin-resistant genera. PCR identification of mcr colistin resistance genes evidenced that mcr-1 was the most prevalent mcr gene and mcr-2 was detected for the first time in pigs from Spanish animal production. The number of samples with mcr-1-carrying bacteria after a freezing period decreased, while real-time quantitation of the mcr-1 gene showed similar values in frozen and fresh samples. Therefore, when frozen cecal samples need to be analyzed, molecular detection of DNA could be the best option to provide a highly representative frame of the initial population present in the sample, and culture-based methods might be a useful complement to study colistin resistance levels.

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Occurrence, distribution, and ecological risk of 21 pharmaceuticals in the Jilin Songhua River were investigated during its freeze-thaw periods, including ice formation, sealed, and breakup. Florfenicol was the most abundant pharmaceutical, with mean concentrations of 123.4 ± 61.1 ng L-1 in water and 73.8 ± 66.3 ng kg-1 in ice. Sulfadiazine occurred at a higher mean concentration in downstream areas (45.6 ± 7.4 ng L-1) than in upstream areas (0.7 ± 0.7 ng L-1). Most pharmaceuticals appeared in relatively high concentrations in water during the ice-breakup period. Complex factors including pharmaceutical usage patterns, ice-regulated photodegradation, biodegradation, water flow, and freeze-concentration effects, as well as the release of pharmaceuticals from ice, were responsible for the temporal variation of pharmaceuticals. Pseudo-ice/water distribution coefficients showed the distribution of pharmaceuticals in ice and demonstrated the effects of their release from the ice on their temporal variations. Most pharmaceuticals posed a risk to algae; of these, amoxicillin exhibited the highest risk. In addition, thawing increased the concentration of thiamphenicol in water, which elevated its ecological risk level. The findings suggest that the pharmaceuticals retained in ice should be considered with regard to regulating pharmaceuticals' temporal variations in seasonal ice-covered rivers during the freeze-thaw process.

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The Biome-BGC (biome biogeochemical cycles) model is widely used for modeling the net primary productivity (NPP) of ecosystems. However, this model ignores soil water changes during the freeze-thaw process in permafrost regions, which may lead to considerable errors in the NPP estimations. In this context we propose a numerical simulation method for improving soil water content during the freeze-thaw process based on the field observation data of soil water and temperature. This approach does not require new parameters and has no impact on other modules. The improvement of soil water content during the freeze-thaw process was then incorporated in the Biome-BGC model for NPP in an alpine meadow in the central Qinghai-Tibetan Plateau (QTP). Interpretation of this data can be found in a research article entitled "An approach for improving soil water content for modeling net primary production on the Qinghai-Tibetan Plateau using Biome-BGC model" (Li et al., 2019).

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It is important to conduct research on the soil freeze⁻thaw process because concurrent adverse effects always occur during this process and can cause serious damage to engineering structures. In this paper, the variation of the impedance signature and the stress wave signal at different temperatures was monitored by using Lead Zirconate Titanate (PZT) transducers through the electromechanical impedance (EMI) method and the active sensing method. Three piezoceramic-based smart aggregates were used in this research. Among them, two smart aggregates were used for the active sensing method, through which one works as an actuator to emit the stress wave signal and the other one works as a sensor to receive the signal. In addition, another smart aggregate was employed for the EMI testing, in which it serves as both an actuator and a receiver to monitor the impedance signature. The trend of the impedance signature with variation of the temperature during the soil freeze⁻thaw process was obtained. Moreover, the relationship between the energy index of the stress wave signal and the soil temperature was established based on wavelet packet energy analysis. The results demonstrate that the piezoceramic-based electromechanical impedance method is reliable for monitoring the soil freezing and thawing process.

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Composite hydrogels as wound dressings feature healing properties in treating wounds. In this study, polyvinyl (alcohol)/chitosan/nano zinc oxide nanocomposite hydrogels were formed using the freeze-thaw method and essential process parameters including thawing time, thawing temperature, and the number of freeze-thaw cycles was investigated to model nanocomposites employing response surface methodology. Critical properties including water vapor transmission rate, porosity, wound fluid absorption, and gel content were modeled using process parameters. Analysis of morphology, mechanical properties, toxicity, protein absorption, antibacterial activity, and in-vitro wound healing were also performed. Results exhibited that increased freeze-thaw cycles caused reduced pore size and increased porosity and wound fluid absorption. Besides, increased freeze-thaw cycles and reduced thawing temperature resulted in increased elastic modulus and tensile strength, while elongation at break point decreased. Antibacterial properties, biocompatibility, and in-vitro wound healing tests demonstrated that the designed system showed no toxicity and it was able to treat the wounds sufficiently.

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The aim of current study was to evaluate effect of rosemary aqueous extract on post-thawed ram sperm quality in a soybean lecithin-based (SL) extender. Ram semen samples were obtained, extended with SL extender and supplemented with 0% (SL-R0), 2% (SL-R2), 4% (SL-R4), 6% (SL-R6), and 8% (SL-R8) rosemary aqueous extract. Following equilibration, the straws were frozen, and then plunged into the liquid nitrogen. After thawing, sperm motility and velocity parameters, plasma membrane functionality, viability, acrosomal and capacitation status were evaluated. Membrane lipid peroxidation was also analyzed through the malondialdehyde (MDA) concentration. Our results showed that SL-R4 and SL-R6 groups resulted in higher (p < 0.05) percentages of total motility, progressive motility, and plasma membrane functionality, as compared with other groups. Highest (p < 0.05) viable and lowest (p < 0.05) dead spermatozoa were observed in SL-R6 group compared to the other groups. The acrosomal and capacitation status were not affected (p > 0.05) by different levels of rosemary aqueous extract. Lower (p < 0.05) MDA concentration has been observed in SL-R4 and SL-R6 groups. The results of this study demonstrate that supplementation of SL extender with rosemary aqueous extract influences post-thawed ram sperm quality in a dose dependent manner.

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