RESUMEN

Ficus carica polysaccharides (FCPS), one of the most effective and important compo-nents in Ficus carica L., had been considered to be a beneficial immunostimulant and may be used in immunotherapy for animals and human. However, studies were little about the effect of FCPS used as immunomodulatory and the suitable dosage in fish. The present study investigated the effect of four different dietary levels of FCPS (0.1%, 0.2%, 0.4%, 0.8%) on the growth performance, innate immune responses and survival of crucian carp against Aeromonas hydrophila infection. The results showed that compared with control group, dietary FCPS had positive effects the growth performance (final weight, feed conversion ratio and survival rate) of crucian carp. FCPS induced significant higher (p < 0.05) leukocyte phagocytosis activity, serum bactericidal activity, lysozyme activity, com-plement C3, SOD activity and total protein level in the serum of crucian carp. Moreover, innate immune response of fish in FCPS groups increased first and then decreased with increasing dietary FCPS from 0.1% to 0.8%, and reached up to the peak in 0.4% dietary FCPS groups. Besides, the cumulative mortalities in FCPS groups were remarkably lower than that of control group when challenged with A. hydrophila, the relative percent survivals were 22.67%, 55.56%, 62.22% and 17.78% in 0.1% group, 0.2% group, 0.4% group and 0.8% group, respectively. These results suggested that dietary FCPS could improve the growth performance, innate immune response and disease resistance against A. hydrophila in fish, and the suitable dietary dose of FCPS was 0.4% in crucian carp.

Asunto(s)

Carpas , Ficus , Enfermedades de los Peces , Infecciones por Bacterias Gramnegativas , Polisacáridos , Aeromonas hydrophila , Animales , Carpas/crecimiento & desarrollo , Carpas/inmunología , Carbohidratos de la Dieta/administración & dosificación , Suplementos Dietéticos , Resistencia a la Enfermedad , Ficus/química , Enfermedades de los Peces/microbiología , Infecciones por Bacterias Gramnegativas/veterinaria , Inmunidad Innata , Polisacáridos/administración & dosificación

RESUMEN

The present study investigated the effect of Ficus carica polysaccharide (FCP), isolated from the fruit of F. carica L., at 0%, 0.1%, 0.5% and 1.0% doses supplementation with feed on genes Interleukin 1-ß (IL-1ß), Tumor Necrosis Factor α (TNF-α) and heat shock protein 70 (HSP70) gene expression in blood, humoral innate immune parameters and resistant to Flavobacterium columnare of grass carp at weeks 1, 2 and 3. The results revealed that administration of FCP significantly (P<0.05) up regulated IL-1ß and TNF-α gene expression. HSP70 gene expression was significantly (P<0.05) lower in FCP-fed fish at the end of trial. The serum total protein, albumin and globulin did not significantly increased in any diet on the first week whereas it was significantly enhanced in 0.5% and 1.0% supplementation diets on weeks 2 and 3 when compared to control. The serum complement C3 was significantly (P<0.05) increased on weeks 1 and 2 when compared to control, however, no significant difference was found in this activity after 3 weeks of treatment. All diets significantly enhanced the serum lysozyme activity, bactericidal activity from weeks 1-2 as compared to control. Grass carp fed with FCP showed remarkably higher resistance against F. columnare (60% survival) compared to the control group (30% survival). These results confirm that FCP can up regulate immune related genes expression, stimulates immune response that per se enhances disease resistance in grass carp.

Asunto(s)

Carpas , Ficus/química , Enfermedades de los Peces/inmunología , Enfermedades de los Peces/microbiología , Infecciones por Flavobacteriaceae/veterinaria , Flavobacterium/efectos de los fármacos , Polisacáridos/farmacología , Animales , Acuicultura/métodos , Proteínas Sanguíneas/metabolismo , Complemento C3/inmunología , Cartilla de ADN/genética , Relación Dosis-Respuesta a Droga , Infecciones por Flavobacteriaceae/inmunología , Regulación de la Expresión Génica/efectos de los fármacos , Regulación de la Expresión Génica/inmunología , Proteínas HSP70 de Choque Térmico/metabolismo , Inmunidad Humoral/efectos de los fármacos , Medicina Tradicional China , Polisacáridos/análisis , Estadísticas no Paramétricas , Factor de Necrosis Tumoral alfa/metabolismo

RESUMEN

Objective: To observe effects of Ficus carica polysaccharide (FCPS) on tumor bearing mice. Methods: The model of S 180 bearing mice and EAC bearing mice were established, tumor cells were inoculated under the skin of right armpit or in abdominal cavity in mice after FCPS being administered orally for ten days, to calculate inhibitive rate of tumor and survial time of mice. Results: FCPS could significantly prevent the growth of tumor, there were no effects on survival time of mice.Conclusion: FCPS had a good antineoplastic effect. It is worth further study.