RESUMEN
We demonstrate a model of chirp-velocity sensitivity in the inferior colliculus (IC) that retains the tuning to amplitude modulation (AM) that was established in earlier models. The mechanism of velocity sensitivity is sequence detection by octopus cells of the posteroventral cochlear nucleus, which have been proposed in physiological studies to respond preferentially to the order of arrival of cross-frequency inputs of different amplitudes. Model architecture is based on coincidence detection of a combination of excitatory and inhibitory inputs. Chirp-sensitivity of the IC output is largely controlled by the strength and timing of the chirp-sensitive octopus-cell inhibitory input. AM tuning is controlled by inhibition and excitation that are tuned to the same frequency. We present several example neurons that demonstrate the feasibility of the model in simulating realistic chirp-sensitivity and AM tuning for a wide range of characteristic frequencies. Additionally, we explore the systematic impact of varying parameters on model responses. The proposed model can be used to assess the contribution of IC chirp-velocity sensitivity to responses to complex sounds, such as speech.
RESUMEN
Due to global rises in temperature, recent studies predict marine species shifting toward higher latitudes. We investigated the impact of interacting abiotic drivers on the distribution potential of the temperate kelp Laminaria hyperborea. The ecosystem engineering species is widespread along European coasts but has not yet been observed in the High Arctic, although it can survive several months of low temperatures and darkness. To investigate its ability to extend northward in future, we conducted a long-term multifactorial experiment with sporophytes from Porsangerfjorden, Norway-close to the species' documented northernmost distribution margin. The samples were exposed to three different photoperiods (PolarDay, LongDay, and PolarNight) at 0°C, 5°C, and 10°C for 3 months. Optimum quantum yield of photosynthesis (Fv/Fm), dry weight, pigments, phlorotannins, and storage carbohydrates were monitored. Both physiological and biochemical parameters revealed that L. hyperborea was strongly influenced by the different photoperiods and their interaction with temperature, while temperature alone exerted only minor effects. The Fv/Fm data were integrated into a species distribution model to project a possible northward expansion of L. hyperborea. The combination of extended day lengths and low temperatures appeared to be the limiting reason for northward spread of L. hyperborea until recently. However, with water temperatures reaching 10°C in summer, this kelp will be able to thrive also in the High Arctic. Moreover, no evidence of stress to Arctic winter warming was observed. Consequently, L. hyperborea has a high potential for spreading northward with further warming which may significantly affect the structure and function of Arctic ecosystems.
RESUMEN
The high cost of producing conventional hybrid cotton seeds led to more research efforts on cotton male sterility systems. There is a lack of studies on cytology, histology, morphological variation, yield, and altered restorer backgrounds to identify and develop male sterility markers in cotton hybrids. Hybrid cotton can be efficiently produced by exploiting genetic male sterility. Among the 19 Genetic Male Sterility (GMS) genes discovered, the lines with ms5ms6 genes are mostly utilised to establish successful hybrid cotton in India. Molecular markers closely associated with the MS alleles are identified to facilitate the efficient and rapid backcrossing of male-sterility genes into elite lines or cultivars by marker-assisted backcrossing. The majority of the markers which are random DNA markers (RDMs), are probably lost, when recombination occurs. In contradiction, molecular markers (functional markers, or FMs) within the genic region can be identified and employed in crops for diverse traits, if prospective characteristic genes are known. In this review, the mechanism of male sterility, its gene expression level, and the need for functional markers for the male sterility trait in cotton have been put forward.
Asunto(s)
Gossypium , Infertilidad Vegetal , Gossypium/genética , Gossypium/fisiología , Infertilidad Vegetal/genética , Marcadores Genéticos , Genes de Plantas/genética , Fitomejoramiento/métodos , Semillas/genética , Regulación de la Expresión Génica de las Plantas/genética , Alelos , Hibridación Genética/genéticaRESUMEN
Microbial remediation of heavy metal (HM)-contaminated soil is a sustainable approach; however, the impact of microbial inoculation on the internal environment of plants remains understudied. Thus, Enterobacter sp. FM-1 (Enterobacter sp.) and the hyperaccumulator Bidens pilosa L. (B. pilosa L.) were used to study these effects. Through analyses of plant physiological and biochemical characteristics, the endophytic microbial community composition, microbial co-occurrence networks and functional predictions, the potential mechanisms by which Enterobacter sp. benefits the phytoremediation of HM-contaminated soil by B. pilosa L. were elucidated. Inoculation with Enterobacter sp. promoted the growth of B. pilosa L. and influenced the endophytic microbial community diversity in B. pilosa L. Interactions among endophytes facilitated the formation of microbial networks, with endophytic fungi playing a more prominent role than endophytic bacteria as the level of HM contamination increased. Functional predictions via PICRUSt2 revealed that endophytic bacteria are involved primarily in processes related to carbohydrate metabolism, ABC transporters, and amino acid metabolism. In conclusion, this study provides evidence for the beneficial role of microbes in improving the plant endosphere environment.
RESUMEN
OBJECTIVES: To investigate the effects of transpalatal (TPA) wire dimension and temporary skeletal anchorage device (TSAD) position on maxillary molar intrusion. MATERIALS AND METHODS: The maxillary molar intrusion measurement system included a maxillary acrylic model, TPA, TSADs, and a three-dimensional Force/Moment (F/M) sensor. The intrusion patterns were categorized into six groups: buccal-mesial, buccal-distal, buccal-mesiodistal, palatal-mesial, palatal-distal, and palatal-mesiodistal. TPA wire dimensions were designed to be 0.7 mm, 0.9 mm, and 1.2 mm. The force and moment loads of the maxillary first molar were measured by the F/M sensor. RESULTS: Single buccal or palatal TSADs induced torquing movement, and single mesial or distal TSADs tended to promote tipping movement. Mesiodistal TSADs would have eliminated tipping, but accentuated torquing movement. The TPA significantly reduced the force and moment experienced by the maxillary first molar along three-dimensional axes. The thicker the TPA wire, the smaller the force and moment to which the maxillary first molar was subjected. CONCLUSIONS: Precise placement of TSADs might have a substantial influence on tooth movement and should be determined in accordance with specific clinical requirements. Increasing the TPA wire dimension could diminish the tipping, torquing, and rotation during TSAD-assisted maxillary molar intrusion, but these tendencies could not be completely eliminated.
Asunto(s)
Maxilar , Diente Molar , Métodos de Anclaje en Ortodoncia , Diseño de Aparato Ortodóncico , Alambres para Ortodoncia , Técnicas de Movimiento Dental , Técnicas de Movimiento Dental/instrumentación , Técnicas de Movimiento Dental/métodos , Métodos de Anclaje en Ortodoncia/instrumentación , Métodos de Anclaje en Ortodoncia/métodos , Humanos , Modelos Dentales , Análisis del Estrés DentalRESUMEN
The piezoelectric properties of two-dimensional semiconductor nanobubbles present remarkable potential for application in flexible optoelectronic devices, and the piezoelectric field has emerged as an efficacious pathway for both the separation and migration of photogenerated electron-hole pairs, along with inhibition of recombination. However, the comprehension and control of photogenerated carrier dynamics within nanobubbles still remain inadequate. Hence, this study is dedicated to underscore the importance of in situ detection and detailed characterization of photogenerated electron-hole pairs in nanobubbles to enrich understanding and strategic manipulation in two-dimensional semiconductor materials. Utilizing frequency modulation kelvin probe force microscopy (FM-KPFM) and strain gradient distribution techniques, the existence of a piezoelectric field in monolayer WS2 nanobubbles was confirmed. Combining w/o and with illumination FM-KPFM, second-order capacitance gradient technique and in situ nanoscale tip-enhanced photoluminescence characterization techniques, the interrelationships among the piezoelectric effect, interlayer carrier transfer, and the funneling effect for photocarrier dynamics process across various nanobubble sizes were revealed. Notably, for a WS2/graphene bubble height of 15.45 nm, a 0 mV surface potential difference was recorded in the bubble region w/o and with illumination, indicating a mutual offset of piezoelectric effect, interlayer carrier transfer, and the funneling effect. This phenomenon is prevalent in transition metal dichalcogenides materials exhibiting inversion symmetry breaking. The implication of our study is profound for advancing the understanding of the dynamics of photogenerated electron-hole pair in nonuniform strain piezoelectric systems, and offers a reliable framework for the separation and modulation of photogenerated electron-hole pair in flexible optoelectronic devices and photocatalytic applications.
RESUMEN
There have been no reports on the quantitative prediction of CYP3A induction-mediated decreases in AUC and Cmax for drug candidates identified as a "victims" of CYP3A induction. Our previous study separately evaluated the fold-induction of hepatic and intestinal CYP3A by known inducers using clinical induction data and revealed that we were able to quantitatively predict the AUC ratio (AUCR) of a few CYP3A substrates in the presence and absence of CYP3A inducers. In the present study, we investigate the predictability of AUCR and also Cmax ratio (CmaxR) in additional 54 clinical studies. The fraction metabolized by CYP3A (fm), the intestinal bioavailability (Fg), and the hepatic intrinsic clearance (CLint) of substrates were determined by the in vitro experiments as well as clinical data used for calculating AUCR and CmaxR. The result showed that 65-69% and 65-67% of predictions were within 2-fold of observed AUCR and CmaxR, respectively. A simulation using multiple parameter combinations suggested that the variability of fm and Fg within a certain range might have a minimal impact on the calculation output. These findings suggest that clinical AUCR and CmaxR of CYP3A substrates can be quantitatively predicted from the preclinical stage.
Asunto(s)
Inductores del Citocromo P-450 CYP3A , Citocromo P-450 CYP3A , Interacciones Farmacológicas , Humanos , Citocromo P-450 CYP3A/metabolismo , Inductores del Citocromo P-450 CYP3A/farmacología , Área Bajo la Curva , Hígado/metabolismo , Hígado/enzimologíaRESUMEN
Hemiparasitic Cassytha filiformis commonly infects native host (Dillenia suffruticosa and Melastoma malabathricum) and introduced host (Acacia auriculiformis and Acacia mangium) species in threatened heath forests in Brunei. This study aims to investigate the impact of parasitism on the ecophysiology of these host species. This study addresses the research gap in understanding the ecophysiology of C. filiformis-host associations, particularly when native and introduced hosts were infected. We generated CO2 and light response curves to examine the effects of increasing CO2 and light levels of infected and uninfected hosts and examined gaseous exchange, mineral nutrients, and secondary bioactive compounds of host-parasite associations. Infected hosts were negatively impacted by C. filiformis as exhibited in the CO2 and light response curves, with C. filiformis-native host association performing better than introduced species. There were no significant differences in photosynthetic parameters between infected and uninfected hosts, except in D. suffruticosa. Certain nutrient contents showed significant differences, but total N, Ca, and K in uninfected hosts were similar to infected hosts. Total phenols and tannins were significantly higher in introduced hosts than native hosts. Our findings asserted that this hemiparasitic vine relies on both its photosynthetic efficiency and nutrient acquisition from its hosts. The parasitism did not significantly hinder the ecophysiological performance of infected hosts, suggesting a plausible co-existence between the hosts and C. filiformis. This study provides essential ecophysiological information for future research on how C. filiformis can establish itself without negatively impacting the co-habitating native hosts.
RESUMEN
Due to the increasing availability of high-quality genome sequences, pan-genomes are gradually replacing single consensus reference genomes in many bioinformatics pipelines to better capture genetic diversity. Traditional bioinformatics tools using the FM-index face memory limitations with such large genome collections. Recent advancements in run-length compressed indices like Gagie et al.'s r-index and Nishimoto and Tabei's move structure, alleviate memory constraints but focus primarily on backward search for MEM-finding. Arakawa et al.'s br-index initiates complete approximate pattern matching using bidirectional search in run-length compressed space, but with significant computational overhead due to complex memory access patterns. We introduce b-move, a novel bidirectional extension of the move structure, enabling fast, cache-efficient bidirectional character extensions in run-length compressed space. It achieves bidirectional character extensions up to 8 times faster than the br-index, closing the performance gap with FM-index-based alternatives, while maintaining the br-index's favorable memory characteristics. For example, all available complete E. coli genomes on NCBI's RefSeq collection can be compiled into a b-move index that fits into the RAM of a typical laptop. Thus, b-move proves practical and scalable for pan-genome indexing and querying. We provide a C++ implementation of b-move, supporting efficient lossless approximate pattern matching including locate functionality, available at https://github.com/biointec/b-move under the AGPL-3.0 license.
RESUMEN
Despite the widespread adoption of k -mer-based methods in bioinformatics, a fundamental question persists: How can we quantify the influence of k sizes in applications? With no universal answer available, choosing an optimal k size or employing multiple k sizes remains application-specific, arbitrary, and computationally expensive. The assessment of the primary parameter k is typically empirical, based on the end products of applications which pass complex processes of genome analysis, comparison, assembly, alignment, and error correction. The elusiveness of the problem stems from a limited understanding of the transitions of k -mers with respect to k sizes. Indeed, there is considerable room for improving both practice and theory by exploring k -mer-specific quantities across multiple k sizes. This paper introduces an algorithmic framework built upon a novel substring representation: the Prokrustean graph. The primary functionality of this framework is to extract various k -mer-based quantities across a range of k sizes, but its computational complexity depends only on maximal repeats, not on the k range. For example, counting maximal unitigs of de Bruijn graphs for k = 10 , , 100 takes just a few seconds with a Prokrustean graph built on a read set of gigabases in size. This efficiency sets the graph apart from other substring indices, such as the FM-index, which are normally optimized for string pattern searching rather than for depicting the substring structure across varying lengths. However, the Prokrustean graph is expected to close this gap, as it can be built using the extended Burrows-Wheeler Transform (eBWT) in a space-efficient manner. The framework is particularly useful in pangenome and metagenome analyses, where the demand for precise multi- k approaches is increasing due to the complex and diverse nature of the information being managed. We introduce four applications implemented with the framework that extract key quantities actively utilized in modern pangenomics and metagenomics.
RESUMEN
The use of fluorescent carbon dots (CDs) as highly precise biolabeling probes has been widespread in the fields of live cell imaging and protein labeling due to their small size and excellent photoluminescence ability to accurately target specific molecules with surface chemical properties. However, there was a lack of research on the interaction between CDs and labeled molecules. In this work, we presented a novel investigation strategy, the fluorescence microscopy-surface plasmon resonance (FM-SPR) system, which combined the use of fluorescence microscopy and wavelength modulation surface plasmon resonance to study the interaction between CDs and labeled molecules in real-time. Using this system, simultaneously recorded the SPR signals and the fluorescence images on the surface of the FM-SPR sensor chip. We observed the dynamic curve and fluorescence images of the interaction between green emissive nitrogen-doped carbon dots (N-CDs) and silk fibroin (SF) in real-time. The kinetic parameters, the quantitative analysis, and the investigation of the binding could be achieved. The results showed a strong linear relationship between the change in SPR signals and the concentration of N-CDs, with a linear coefficient of 0.99913. The linear detection range was 2.5 µg/mL-100 µg/mL, and the real lowest detection limit reached 0.5 µg/mL. Additionally, the green fluorescence points in the imaging region on the FM-SPR sensor chip increased with the concentration of N-CDs, which was consistent with the change in SPR signals. Using this system we also acquired the association rate and dissociation rate of N-CDs to SF which were 2.65 × 10-5/s and 1.52 × 10-5/s, respectively. This demonstrated the effectiveness of our method in quantitatively analyzing SF labeled with N-CDs.
Asunto(s)
Carbono , Fibroínas , Microscopía Fluorescente , Puntos Cuánticos , Resonancia por Plasmón de Superficie , Fibroínas/química , Resonancia por Plasmón de Superficie/métodos , Carbono/química , Puntos Cuánticos/química , Microscopía Fluorescente/métodos , Colorantes Fluorescentes/química , Animales , Límite de Detección , CinéticaRESUMEN
Background: Fibrosing mediastinitis (FM) secondary to atypical sarcoidosis (atypical presentation of sarcoidosis) is rarely reported at home and abroad. Its clinical manifestations represent a lack of specificity, and the initial diagnosis is frequently difficult. In particular, this case has multiple pulmonary nodules with mediastinal lymph node enlargement and bilateral pleural effusion, and pulmonary fibrosis still exists after treatment, which is inconsistent with any clinical stage of pulmonary sarcoidosis, further increasing the diagnostic difficulty. We retrospectively analyzed the clinical data of a case of FM secondary to atypical sarcoidosis diagnosed by endobronchial ultrasound-guided cautery-assisted transbronchial mediastinal cryobiopsy (EBUS-CA-TBMCB) in Chongqing University Fuling Hospital, to improve clinicians' attention to FM and understand that EBUS-CA-TBMCB remains an effective way of etiological diagnosis. Case Description: A 70-year-old man was hospitalized with cough and dyspnea for two months. After admission, through chest computed tomography (CT), ultrasound guided bilateral lung biopsy, left parietal pleural biopsy, and EBUS-CA-TBMCB, the final diagnosis was atypical sarcoidosis secondary FM. After taking glucocorticoid orally, the patient's condition improved significantly, and was discharged from the hospital. We continued following up outside the hospital, and the patient's condition was further improved. Conclusions: The diagnosis of FM is mainly based on typical imaging manifestations. When the contrast-enhanced chest CT finds localized or diffuse soft tissue density shadows around the mediastinum and pulmonary hilum with an irregular shape, with or without calcification, particular attention should be paid to exclude FM. EBUS-CA-TBMCB, as an improved minimally invasive method, can obtain enough tissue samples for pathological diagnosis, which may be the effective biopsy method for the etiology of FM to avoid missed diagnosis and misdiagnosis in the future.
RESUMEN
The study aims to gauge the impact of economic policy uncertainty, ICT, and environmental tax on environmental sustainability, which is measured by carbon emission and ecological footprint in a panel of 22 nations from 1997 to 2021. The present study has implemented the advanced panel data estimation techniques, including continuously updated fully modified (CUP-FM) and continuously updated bias-corrected (CUP-BC), dynamic seemingly unrelated regressions (DSUR), and nonlinear autoregressive distributed lagged (NARDL) in documenting the elasticities of target variables. Moreover, the directional causality has been tested through the D-H causality test. Study findings documented a positive and statistically significant linkage between EPU and environmental degradation. That is, EPU amplifies the emission of CO2 and ecological instability. The effects of ET and ICT are positively associated with environmental sustainability; that is, ET and ICT control the emission of CO2 and bring ecological improvement. This study contributes to the existing body of literature by conducting a thorough analysis of the relationship between various factors and their impact on environmental degradation. The study emphasizes the significance of every factor in influencing environmental outcomes. It provides policy suggestions to reduce CO2 emissions and promote ecological sustainability. The findings add valuable insights to the ongoing conversation about how to tackle environmental challenges in our constantly evolving world.
Asunto(s)
Política Ambiental , Impuestos , Incertidumbre , Conservación de los Recursos Naturales , Dióxido de Carbono/análisis , Desarrollo SostenibleRESUMEN
BACKGROUND: The vasoconstrictor effects of angiotensin II via type 1 angiotensin II receptors in vascular smooth muscle cells are well established, but the direct effects of angiotensin II on vascular endothelial cells (VECs) in vivo and the mechanisms how VECs may mitigate angiotensin II-mediated vasoconstriction are not fully understood. The present study aimed to explore the molecular mechanisms and pathophysiological relevance of the direct actions of angiotensin II on VECs in kidney and brain microvessels in vivo. METHODS AND RESULTS: Changes in VEC intracellular calcium ([Ca2+]i) and nitric oxide (NO) production were visualized by intravital multiphoton microscopy of cadherin 5-Salsa6f mice or the endothelial uptake of NO-sensitive dye 4-amino-5-methylamino-2',7'-difluorofluorescein diacetate, respectively. Kidney fibrosis by unilateral ureteral obstruction and Ready-to-use adeno-associated virus expressing Mouse Renin 1 gene (Ren1-AAV) hypertension were used as disease models. Acute systemic angiotensin II injections triggered >4-fold increases in VEC [Ca2+]i in brain and kidney resistance arterioles and capillaries that were blocked by pretreatment with the type 1 angiotensin II receptor inhibitor losartan, but not by the type 2 angiotensin II receptor inhibitor PD123319. VEC responded to acute angiotensin II by increased NO production as indicated by >1.5-fold increase in 4-amino-5-methylamino-2',7'-difluorofluorescein diacetate fluorescence intensity. In mice with kidney fibrosis or hypertension, the angiotensin II-induced VEC [Ca2+]i and NO responses were significantly reduced, which was associated with more robust vasoconstrictions, VEC shedding, and microthrombi formation. CONCLUSIONS: The present study directly visualized angiotensin II-induced increases in VEC [Ca2+]i and NO production that serve to counterbalance agonist-induced vasoconstriction and maintain residual organ blood flow. These direct and endothelium-specific angiotensin II effects were blunted in disease conditions and linked to endothelial dysfunction and the development of vascular pathologies.
Asunto(s)
Angiotensina II , Encéfalo , Calcio , Hipertensión , Riñón , Microvasos , Óxido Nítrico , Vasoconstricción , Animales , Ratones , Angiotensina II/farmacología , Encéfalo/metabolismo , Encéfalo/irrigación sanguínea , Calcio/metabolismo , Señalización del Calcio/efectos de los fármacos , Modelos Animales de Enfermedad , Células Endoteliales/metabolismo , Células Endoteliales/efectos de los fármacos , Hipertensión/metabolismo , Hipertensión/fisiopatología , Hipertensión/tratamiento farmacológico , Riñón/irrigación sanguínea , Riñón/metabolismo , Ratones Endogámicos C57BL , Microvasos/metabolismo , Microvasos/efectos de los fármacos , Microvasos/patología , Óxido Nítrico/metabolismo , Vasoconstricción/efectos de los fármacosRESUMEN
Since there is an upper limit to skill improvement through the repetition of actual execution, we examined whether motor imagery could be used in combination with actual execution to maximize motor skill improvement. Fingertip dexterity was evaluated in 25 healthy participants performing a force adjustment task using a pinch movement with the left thumb and index finger. In the intervention condition, six sets of repetitions of combined actual execution and motor imagery were performed, while in the control condition, the same flow was performed, but with motor imagery replaced by rest. Changes in the excitability of spinal motoneurons during motor imagery compared to rest were compared in terms of the F/M amplitude ratio. Motor skill changes were compared before and after repeated practice and between the conditions, respectively, using the absolute amount of adjustment error between the target pinch force value and the delivered pinch force value (absolute error) as an index. The results showed that the repetition of exercise practice and motor imagery decreased the absolute error, which was greater than that of exercise practice alone in terms of motor skill improvement. The F/M amplitude ratio for motor imagery compared to rest did not increase. This suggests that motor imagery is involved in the degree of the increase of spinal motoneuron excitability based on the real-time prediction of motor execution and that there may be no need for an increase in excitability during motor skill control.
RESUMEN
Technological innovation is a critical element of economic and environmental sustainability; thus, the promotion of technological innovation in the economy has gained an apex among policy makers. The study's impetus is to measure the effect of investments in information and communication technology (ICT), education, and political stability on technical innovation in BRI countries for 2004-2020. In the process of documenting the empirical nexus through the implementation of novel panel techniques commonly known as Dynamic Seemingly Unrelated Regressions (SUR), continuously updated fully modified" (Cup-FM) and continuously updated bias-corrected (Cup-BC). The results of the slope of heterogeneity, cross-sectional dependency test, and panel cointegration test have revealed the presence of heterogeneity, all the research variables possessed certain common dynamics, and, most importantly presence of long-run association. The study documented the coefficients of ICT, education and Political stability are positive and statistically significant, indicating a contributory effect in fostering technological innovation in BRI nations. The findings emphasize the importance of upholding political stability, directing resources toward education, and fostering an environment that encourages innovation through the integration of information and communication technology (ICT). The study also highlights how critical it is to bring in FDI and use it to your advantage in order to boost tech development and the economy.
RESUMEN
Microplastics (MPs) pose a significant threat to the function of agro-ecosystems. At present, research on MPs has mainly focused on the effects of different concentrations or types of MPs on a crop, while ignoring other environmental factors. In agricultural production, the application of nitrogen (N) fertilizer is an important means to maintain the high yield of crops. The effects of MPs and N on growth parameters, photosynthetic system, active oxygen metabolism, nutrient content, and ascorbate-glutathione (AsA-GSH) cycle of maize and wheat were studied in order to explicit whether N addition could effectively alleviate the effects of MPs on maize and wheat. The results showed that MPs inhibited the plant height of both maize and wheat, and MPs effects on physiological traits of maize were more severe than those of wheat, reflecting in reactive oxygen metabolism and restriction of photosynthetic capacity. Under the condition of N supply, AsA-GSH cycle of two plants has different response strategies to MPs: Maize promoted enzyme activity and co-accumulation of AsA and GSH, while wheat tended to consume AsA and accumulate GSH. N application induced slight oxidative stress on maize, which was manifested as an increase in hydrogen peroxide and malonaldehyde contents, and activities of polyphenol oxidase and peroxidase. The antioxidant capacity of maize treated with the combination of MPs + N was better than that treated with N or MPs alone. N could effectively alleviate the adverse effects of MPs on wheat by improving the antioxidant capacity.
Asunto(s)
Microplásticos , Nitrógeno , Oxidación-Reducción , Fotosíntesis , Triticum , Zea mays , Zea mays/efectos de los fármacos , Zea mays/crecimiento & desarrollo , Zea mays/fisiología , Triticum/crecimiento & desarrollo , Triticum/fisiología , Triticum/efectos de los fármacos , Triticum/metabolismo , Fotosíntesis/efectos de los fármacos , Nitrógeno/metabolismo , Microplásticos/toxicidad , Glutatión/metabolismo , Fertilizantes , Homeostasis , Contaminantes del Suelo/toxicidad , Ácido Ascórbico/metabolismo , Estrés OxidativoRESUMEN
FM-indexes are crucial data structures in DNA alignment, but searching with them usually takes at least one random access per character in the query pattern. Ferragina and Fischer [1] observed in 2007 that word-based indexes often use fewer random accesses than character-based indexes, and thus support faster searches. Since DNA lacks natural word-boundaries, however, it is necessary to parse it somehow before applying word-based FM-indexing. In 2022, Deng et al. [2] proposed parsing genomic data by induced suffix sorting, and showed that the resulting word-based FM-indexes support faster counting queries than standard FM-indexes when patterns are a few thousand characters or longer. In this paper we show that using prefix-free parsing-which takes parameters that let us tune the average length of the phrases-instead of induced suffix sorting, gives a significant speedup for patterns of only a few hundred characters. We implement our method and demonstrate it is between 3 and 18 times faster than competing methods on queries to GRCh38, and is consistently faster on queries made to 25,000, 50,000 and 100,000 SARS-CoV-2 genomes. Hence, it seems our method accelerates the performance of count over all state-of-the-art methods with a moderate increase in the memory. The source code for PFP - FM is available at https://github.com/AaronHong1024/afm .
RESUMEN
AIM: The diagnoses of Chronic Fatigue Syndrome (CFS) and Fibromyalgia (FM) are highly associated with fatigue and pain, respectively. Physiologically and clinically an effect of thyroid status on fatigue and pain is expected. There may be clinically relevant differences in thyroid hormone axes though within values of reference in both patients with normal thyroid hormones, or in patients with well-regulated thyroid disease. These potential differences are explored in this study. MATERIALS AND METHODS: In the present study, female patients with CFS (n = 49) and FM (n = 58) as well as female healthy controls (n = 53) were included. We explored plasma levels of TSH and FT4 between the groups using Kruskall-Wallis, and the relation between fatigue score and levels of TSH and FT4 by means of Spearman's rho. RESULTS: There were no group differences between CFS patients, FM patients, and healthy controls in levels of TSH and FT4. CONCLUSION: As one might clinically and physiologically expect an association between thyroid function and fatigue, which may be associated with clinical disorders such as CFS and FM, we suggest future studies to examine the field further by exploring the influence of thyroid receptors and responses of the thyroid hormone cascade.