RESUMEN
Ground experimentation is an important method to verify the performance and quality of spacecraft structures. How to simulate the force and heat fields that a spacecraft may be subjected to during flight is a challenge for experiment design. In this paper, the static strength experimental method of spacecraft in the combined force and heat fields is studied. Furthermore, the coupling cases of the spacecraft during the flight process are simulated using an airbag and a resistive heater. Finally, it can be concluded that the accuracy of the proposed experimental method as well as the structural strength of the spacecraft can meet technical requirements during flight.
RESUMEN
The T-box (Tbx) proteins have a 180-230 amino acid DNA-binding domain, first reported in the Brachyury (T) protein. They are highly conserved among metazoans. They regulate a multitude of cellular functions in development and disease. Here, we report posttranscriptional and translational regulation of midline (mid), a Tbx member in Drosophila. We found that the 3'UTR of mid has mRNA degradation elements and AT-rich sequences. In Schneider S2 cells, mid-mRNA could be detected only when the transgene was without the 3'UTR. Similarly, the 3'UTR linked to the Renilla luciferase reporter significantly reduced the activity of the Luciferase, whereas deleting only the degradation elements from the 3'UTR resulted in reduced activity, but not as much. Overexpression of mid in MP2, an embryonic neuroblast, showed no significant difference in the levels of mid-mRNA between the 2 transgenes, with and without the 3'UTR, indicating the absence of posttranscriptional regulation of mid in MP2. Moreover, while elevated mid-RNA was detected in MP2 in nearly all hemisegments, only a fifth of those hemisegments had elevated levels of the protein. Overexpression of the 2 transgenes resulted in MP2-lineage defects at about the same frequency. These results indicate a translational/posttranslational regulation of mid in MP2. The regulation of ectopically expressed mid in the wing imaginal disc was complex. In the wing disc, where mid is not expressed, the ectopic expression of the transgene lacking the 3'UTR had a higher level of mid-RNA and the protein had a stronger phenotypic effect. These results indicate that the 3'UTR can subject mid-mRNA to degradation in a cell- and tissue-specific manner. We further report a balancer-mediated transgenerational modifier effect on the expression and gain of function effects of the 2 transgenes.
Asunto(s)
Regiones no Traducidas 3' , Proteínas de Drosophila , Proteínas de Dominio T Box , Animales , Proteínas de Drosophila/genética , Proteínas de Drosophila/metabolismo , Proteínas de Dominio T Box/genética , Proteínas de Dominio T Box/metabolismo , Estabilidad del ARN/genética , Drosophila melanogaster/genética , ARN Mensajero/genética , ARN Mensajero/metabolismo , Regulación del Desarrollo de la Expresión Génica , Procesamiento Postranscripcional del ARN , Especificidad de Órganos , Alas de Animales/metabolismo , Alas de Animales/crecimiento & desarrollo , Línea Celular , Drosophila/genética , Drosophila/metabolismoRESUMEN
In order to adapt to the education of the professional who work in thecurrent medical lab,we set up the course of'Experimental Technology of Medical Function'. It is a required subject for the professional with laboratory skill. This article explores the curriculum sys-tem,teaching contents,teaching methods and examination.