RESUMEN

Somatic embryogenesis is a powerful tool both for cloning and studies of genetic transformation and embryo development. Most protocols for somatic embryogenesis induction start from zygotic embryos or embryonic-derived tissues which do not allow the propagation of elite trees. In the present study, a reliable protocol for somatic embryogenesis induction from adult trees of strawberry tree is described. Leaves from in vitro proliferating shoots were used to induce somatic embryo formation on a medium containing an auxin and a cytokinin. Somatic embryos germinated in a plant growth regulator-free medium.

Asunto(s)

Fragaria/crecimiento & desarrollo , Brotes de la Planta/crecimiento & desarrollo , Técnicas de Embriogénesis Somática de Plantas/métodos , Técnicas de Cultivo de Tejidos/métodos , Fragaria/genética , Frutas/genética , Frutas/crecimiento & desarrollo , Desarrollo de la Planta/genética , Brotes de la Planta/genética , Plantas Modificadas Genéticamente/genética , Regeneración/genética