RESUMEN

The protein-lipid overlay assay is an inexpensive, easy-to-implement, and high-throughput methodology that employs nitrocellulose membranes to immobilize lipids in order to rapid screen and identify protein-lipid interactions. In this chapter, we show how this methodology can identify potential modulators of protein-lipid interactions by screening water-soluble lipid competitors or even the introduction of pH changes during the binding assay to identify pH-dependent lipid binding events.

Asunto(s)

Metabolismo de los Lípidos , Fosfolípidos/química , Proteínas/química , Sitios de Unión , Colodión/química , Evaluación Preclínica de Medicamentos/métodos , Glutatión Transferasa/química , Humanos , Concentración de Iones de Hidrógeno , Péptidos y Proteínas de Señalización Intracelular/química , Ligandos , Unión Proteica , Proteínas de Transporte Vesicular/química