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Microplastics (MPs) constitute the predominant plastic type in marine environments. Since they occupy the same size fraction of sediment particles and planktonic organisms they are potentially bioavailable to a broad scope of organisms, such as filter feeders, which are particularly vulnerable to MP ingestion. To understand the potential impact of MPs in filter feeders it is essential to clarify the uptake, accumulation patterns and elimination rates with time of MPs. The aim of this study was to determine the depuration dynamics and accumulation in tissues of mussels Mytilus galloprovincialis exposed during 24 h to different size polystyrene MPs (1 µm and 10 µm), and depurated for a maximum of 7 days (T = 24 h, T = 48 h and T = 7 d). Mussels were chemically digested with KOH 10% and filtered to quantify the number of MP ingested, and they were cryostat sliced for MP localization in tissues. Both MP sizes were quantified in all depuration times, but mussels accumulated significantly higher quantities of 10 µm MP throughout depuration compared to 1 µm MP. A significant decrease was observed after 7 d depuration in mussels exposed to 10 µm. Mussels removed the same amount of 1 and 10 µm MP after 7 days depuration. However, the depuration dynamics differed for each size-MPs and showed to be size-dependent. Most of both size MPs were eliminated in the first 24 h, but 1 µm MP showed to pass faster through the digestive tract than 10 µm MP. MPs of 1 µm and 10 µm were localized mainly in the lumen and a few in the epithelium of the digestive tract (stomach, intestine and digestive gland) during the depuration and in the gills after the exposure; as confirmed by Raman spectroscopy. The usage of chemical digestion and histological analysis as complementary techniques show to be suitable to infer the depuration dynamics of MPs in mussels.

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Two burrowing clam species, namely Meretrix meretrix and Paphia undulata, were offered two sizes (small: 45-53 µm, and large: 106-125 µm) of fluorescent red polyethylene microbeads, and the ingestion (number of MPs in the body tissue and faeces) and rejection (number of MPs in pseudofaeces) of MPs investigated. Overall, MP beads ingested were 36 % more than those rejected. There was also a significant interaction between the size and fate of MPs. For both species, significantly more small beads were ingested than rejected, but there was no difference for the large beads. P. undulata ingested more MPs than M. meretrix and both species could depurate all the ingested MPs in 72 h, although a longer time was needed for the former species. The results can provide guidance on seafood selection and pre-treatment to minimize the number of MPs ingested by humans.

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Marine toxins pose a significant safety risk, leading to human intoxications and causing substantial economic losses in seafood-producing regions. The development of rapid, cost-effective, efficient, and reliable approaches for the containment of these substances is therefore crucial in order to mitigate the adverse impact of marine toxins. This research conducted a comprehensive review on the toxicity and influencing factors of marine toxins production. Additionally, depuration technologies, including adsorption, advanced oxidation processes, biodegradation, heating treatment, temporary maintenance and purification, and drug inhibition, were systematically summarized. The study also provided a comparative analysis of the advantages and disadvantages of various depuration technologies and proposed strategies for future development.

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Microplastic risk assessment often characterizes primary plastics, plastics intentionally manufactured at the micro- and nanoscale, or plastics collected within the natural environment, which often lack repeatability and the volume necessary for reliable hazard characterization. There is limited understanding of how environmentally representative plastics prepared at the microscale impact aquatic organisms. The aim of the present study was to create environmentally representative microplastics and characterize their toxicokinetics and hazards. Plastic cups and forks were micronized to <120 µm particles and Daphnia magna were exposed for 48 h at concentrations ranging from 0.01 mg/L to 100 mg/L. Uptake and depuration experiments were conducted at the highest concentration where accumulation was confirmed. Raman spectroscopy identified that both plastics were polystyrene and had similar size distributions. Microplastics were not acutely toxic but accumulated and rapidly depurated. Toxicokinetics demonstrated that cup MPs were consumed at higher rates than fork MPs despite similar physical characteristics. Daphnia magna preferentially selected smaller particles from the heterogenous suspensions. Future research will need to further explore the relationship between physicochemical properties, particularly size, and ecotoxicity. The study focused on mortality as the primary hazard endpoint. However alternative, sublethal biomarkers may be more appropriate in describing the effects of microplastic exposure.

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Direct ingestion of micro/nanoplastics (MNPs) results in significant accumulation in gastrointestinal (GI) tract of fish. The breathing process of fish makes MNPs easily retained in their gills. However, the uptake of MNPs in other fish organs remains largely unknown, let alone their kinetic processes. Herein, microplastics (MPs) and nanoplastics (NPs) in vivo imaging and precise quantification in various tissues (GI tract, gill, liver, brain, eye, and skin) of seawater (SW)- and freshwater (FW)- acclimated medaka Oryzias melastigma were achieved at an environmentally relevant concentration. Subsequently, the distribution kinetics of MNPs was investigated over a 96-h uptake and 48-h depuration period. MNPs were quickly and mostly captured in GI tract and gill of O. melastigma, and then transferred to liver and brain likely via blood circulation. Such transport was more efficient for NPs as compared to MPs, as evidenced by the consistently higher bioconcentration factors in both SW and FW conditions. The detection of MNPs in eye and skin of O. melastigma was more of an adsorption process, although the specific mechanisms of adsorption and absorption process can hardly be clearly differentiated. This study presented distribution kinetics of MNPs in O. melastigma and highlighted their possible transportation among tissues.

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The toxic effects of tire wear particles (TWPs) in the environment are a growing concern for a variety of aquatic organisms. However, studies about TWPs toxicity on aquatic organisms are limited. This study investigated the accumulation and depuration of TWPs in zebrafish at three different concentrations (5 mg/L, 10 mg/L, and 20 mg/L), as well as the toxic effects on the gill, liver, and gut. We found that TWPs could accumulate in the gill and gut for a long time, and the number of TWPs at the high-concentration (20 mg/L) was higher than at the low-concentration (5 mg/L). TWPs induced oxidative stress in the gill and liver. The liver transcriptome profiles indicated that the high concentration of TWPs tended to up-regulate metabolic processes, whereas the low concentration of TWPs was inclined to down-regulate cellular processes. The high-concentration treatment significantly increased xenobiotic biodegradation and metabolism, and lipid metabolism-related pathways, whereas the low-concentration treatment distinctly altered amino acid metabolism-related pathways. The expression of gstt1b, ugt1a1, mgst3b, miox, hsd17b3, and cyp8b1 gene was up-regulated in all TWPs treatments. In addition, Gemmobacter and Shinella enriched in the high-concentration treatment were closely correlated with the degradation of TWPs. These findings provided objective evidence for the toxicity evaluation of TWPs on zebrafish.

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Benzo[a]pyrene (BaP), a representative five-membered polycyclic aromatic hydrocarbon, has been extensively studied as a pollutant for decades. Despite this, sex-specific responses to BaP exposure remain poorly understood. This study employed a life-cycle exposure approach to investigate the effects of prolonged BaP exposure on marine medaka (Oryzias melastigma), highlighting sex-specific responses. After a 90-day exposure period, significant variations in biometric measurements and oxidative stress markers were observed between male and female fish. BaP exposure resulted in weak detoxification defense in males, while females exhibited an opposite response. Transcriptomic analysis revealed 13 significantly enriched pathways in males and 11 in females, with varying numbers of differentially expressed genes between the sexes, highlighting distinct biological responses. Host resistance assay showed higher mortality rates among BaP-exposed males, and suppressed immune gene expressions and lysozyme activity, while females demonstrated enhanced immune genes and lysozyme activity post-challenge, indicating a more resilient defense response. Furthermore, after a one-month depuration period following BaP exposure, male medaka demonstrated slower recoverability compared to females. These findings underscore sex-specific effects of BaP exposure on fish, with females displaying stronger resilience. Understanding these distinctions are crucial for accurately assessing the impact of environmental pollutants on the aquatic population and ecosystem maintenance.

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We evaluated the physiological characteristics of chemical-tolerant cladocerans. Over the course of 26 generations (F25), Daphnia magna was continuously exposed to pirimicarb (carbamate) solutions (0, 3.8, 7.5, and 15 µg/L) in sub-lethal or lethal levels. The 48 h EC50 values (29.2-29.9 µg/L) for 7.5 and 15 µg/L exposure groups were found to be nearly two times higher than that in the control (17.2 µg/L). Subsequently, we investigated whether the extinction probability changed when the chemical-tolerant daphnids were fed two different types of food, Chlorella vulgaris and Synechococcus leopoliensis. Furthermore, we ascertained how chemical tolerance influences respiration and depuration rates. The 48 h EC50 value was positively related to the extinction probability when the daphnids were fed S. leopoliensis. Because the measured lipid content of S. leopoliensis was three times lower than that of C. vulgaris, the tolerant daphnids struggled under nutrient-poor conditions. Respiration rates across all pirimicarb treatment groups were higher than those in the control group, suggesting that they may produce large amounts of energy through respiration to maintain the chemical tolerance. Since the pirimicarb depuration rate for 7.5 µg/L exposure groups was higher than that in the control, the altered metabolic/excretion rate may be one factor for acquiring chemical tolerance. These altered physiological characteristics are crucial parameters for evaluating the mechanisms of chemical tolerance and associated fitness costs.

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Microplastics are ubiquitous in the aquatic environment, and bivalves such as the Eastern oyster (Crassostrea virginica) can accumulate these particles directly from the water column. Bivalves are concurrently exposed to pathogenic and toxin-producing bacteria, including Vibrio spp. and Microcystis spp., which have been shown to adversely impact filtration rates. Exposure to these bacteria could thus affect oysters' ability to accumulate and depurate microplastics. As climate change creates conditions that favor Vibrio spp. and Microcystis spp. growth in estuaries, it is increasingly important to understand how these co-occurring biotic stressors influence microplastic contamination in bivalves. The objective of this study was to examine how co-exposures to Vibrio vulnificus and Microcystis aeruginosa influence microplastic accumulation and depuration in Eastern oysters. Oysters were exposed to nylon microplastics (5000 particles L-1) and either V. vulnificus, M. aeruginosa, or both species (104 colony-forming units or cells mL-1, respectively) and sampled over time up to 96 h. Following exposure, remaining oysters were allowed to depurate in clean seawater and sampled over time for up to 96 h. Microplastic concentrations in oysters were quantified and compared among treatments, and rate constants for uptake (ku) and depuration (kd) were calculated using nonlinear regression and two-compartment kinetic models. Overall, microplastic concentrations in oysters exposed to V. vulnificus (X‾ = 2.885 ± 0.350 (SE) particles g-1 w.w.) and V. vulnificus with M. aeruginosa (X‾ = 3.089 ± 0.481 particles g-1 w.w.) were higher than oysters exposed to M. aeruginosa (X‾ = 1.540 ± 0.235 particles g-1 w.w.) and to microplastics alone (X‾ = 1.599 ± 0.208 particles g-1 w.w.). Characterizing microplastic accumulation and depuration in oysters co-exposed to these biotic stressors is an important first step in understanding how contaminant loads in bivalves can change. With this research, the efficacy of depuration for commonly-consumed seafood species can be estimated.

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The global interest in edible insects as sustainable protein sources raises concerns about the bioaccumulation of contaminants, including polycyclic aromatic hydrocarbons (PAHs), to problematic levels. Understanding the accumulation dynamics of PAHs in edible insects is highly relevant due to the widespread sources and toxicological profiles; however, the bioaccumulative potential of PAHs in edible insects is unexplored. This study examined the uptake and elimination dynamics of benzo(a)pyrene (B(a)P), a representative and carcinogenic PAH, in yellow mealworm larvae (YMW, Tenebrio molitor). Larvae were exposed to feeding substrate with varying B(a)P concentrations (0.03, 0.3, and 3 mg kg-1), and uptake (21 days in B(a)P-contaminated substrate) and elimination (21 days in B(a)P-free substrate) kinetics were subsequently assessed. The results showed that YMW can eliminate B(a)P, revealing dose-dependent B(a)P bioaccumulation in these insects. Larvae fed on a substrate with 0.03 mg kg-1 accumulated B(a)P over 21 days, presenting values of 0.049 (Standard deviation - 0.011) mg kg-1 and a kinetic-based (BAFkinetic) of 1.93 g substrate g organism-1, exceeding the EU regulatory limits for food. However, with a B(a)P half-life (DT50) of 4.19 days in the larvae, an EU legislation safety criterion was met after a 13-day depuration period in clean substrate. Larvae exposed to substrates with 0.3 and 3 mg kg-1 showed B(a)P accumulation, with BAFkinetic values of 3.27 and 2.09 g substrate g organism-1, respectively, not meeting the current legal standards for food consumption at the end of the exposure to B(a)P. Although the B(a)P half-life values after 35 days were 4.30 and 10.22 days (DT50s), the larvae retained B(a)P levels exceeding permitted food safety limits. These findings highlight a significant oversight in regulating PAHs in animal feed and the need for comprehensive safety evaluations of PAH hazards in edible insects for improved PAH feeding guidelines.

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It has been demonstrated that microplastics (MPs) may be inadvertently ingested by aquatic animals, causing harm to their physiological functions and potentially entering the food chain, thereby posing risks to human food safety. To achieve an environmentally friendly and efficient reduction of MPs in freshwater environments, this experiment investigates the depuration effect of C. demersum on MPs using three common aquatic animals: Macrobrachium nipponense, Corbicula fluminea, and Bellamya aeruginosa as research subjects. The amounts of MPs, digestive enzyme activity, oxidative stress index, and energy metabolism enzyme activity in the digestive and non-digestive systems of three aquatic animals were measured on exposure days 1, 3, and 7 and on depuration days 1 and 3. The results indicated that the depuration effect of C. demersum and the species interaction were significant for the whole individual. Concerning digestive tissue, C. demersum was the most effective in purifying B. aeruginosa. When subjected to short-term exposure to MPs, C. demersum displayed a superior depuration effect. Among non-digestive tissues, C. demersum exhibited the earliest purifying effect on C. fluminea. Additionally, C. demersum alleviated physiological responses caused by MPs. In conclusion, this study underscores C. demersum as a promising new method for removing MPs from aquatic organisms.

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Arbuscular mycorrhizal fungi (AMF) have different biological mechanisms to alleviate stressful conditions in heavy metals (HMs) polluted soil. These mechanisms were widely assessed under controlled/greenhouse conditions, but scarcely studied at pilot or territory scale. The aim of this study was to evaluate the response of two Rhizophagus intraradices strains isolated from soils with different histories of pollution, in association with Senecio bonariensis plants, growing in an engineering vegetal depuration module filled with artificially HMs polluted substrate. Plants inoculated with GC3 strain uptook low amounts of HMs and translocated them to shoot biomass. Heavy metals (Mg, Zn, Mn, Cr, Cu and Ni) and macronutrients (Ca, K, S and P) were accumulated in roots of S. bonariensis when inoculated with GB8 strain, limiting their translocation to the shoot. Uninoculated plants showed high translocation of all studied elements to shoot tissues. Concluding, tested R. intraradices strains have exhibited different phytoprotection mechanisms under extremely toxic concentrations of HMs. Moreover, the development of the assay at such a high Technological Readiness Level represents a novel contribution in this field of study.

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To follow up field observations in the Chornobyl Exclusion Zone (ChEZ), a series of controlled model aquarium experiments were conducted to determine the uptake and depuration rates of 137Cs and 90Sr in silver Prussian carp (Carassius gibelio) in fresh water, varying in temperature from 5 to 27 °C, with daily feeding rates of 0-1.5 % fish weight day-1. In the present study, the 137Cs uptake rates in muscle tissues directly from water, 0.05-0.09 day-1 at temperatures of 5-27 °C, were significantly lower than previously reported for fish fed under natural conditions in contaminated lakes within the ChEZ. The rate of 90Sr uptake in bone tissues of silver Prussian carp varied from 0.055 day-1 at a water temperature of 5 °C and feeding rates ≤0.15 % fish weight day-1 to 1.5 ± 0.2 day-1 at a temperature of 27 ± 1 °Ð¡ and at the highest tested feeding rate of 1.5 % day-1. The rate of decrease of 137Cs concentration in muscle tissues was kb = 0.0028 ± 0.0004 day-1 (T1/2 = 248 ± 35 days) at the lowest water temperature tested (5 °Ð¡). At water temperatures between 13 and 26 °Ð¡ and a feeding rate of 0.15 % day-1, the rate increased to kb = 0.0071-0.0092 day-1 (T1/2 = 75-99 days). The rates of decrease of 90Sr activity concentration in bone tissues at water temperatures between 22 and 25 °Ð¡ and a feeding rate of 0.5 % day-1 were kb=0.004-0.0014 day-1, and the associated biological half-life T1/2 ranged 50-160 days, respectively. The present work supported conclusions related to the main pathways of 137Cs and 90Sr uptake by silver Prussian carp, and demonstrated the usefulness of combining field and laboratory uptake and depuration experiments.

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Earthworm could regulate their body concentration of arsenic via storage or excretion, and the ability of As efflux among different earthworms is not consistent. Here, whole and semi As exposure patterns with 0-10-30-60-100 mg kg-1 exposure concentrations were set to characterize the As efflux in geophagous earthworm, Metaphire guillelmi. Cast As (As-C) and earthworms' antioxidative responses were monitored to explore the efflux mechanisms under 30 mg kg-1 As-spiked soil (As30), besides, As concentration in earthworm tissue after egestion and dissection depurations were compared. In the whole exposure pattern, As concentration in gut content (As-G, 19.2-120.3 mg kg-1) surpassed that in the tissue (As-T, 17.2-53.2 mg kg-1), and they both increased with exposure concentrations. With the prolong time, they firstly increased and kept stable between day 10-15, then As-G increased while As-T decreased between day 15-20. In the semi-exposure pattern, both As-G and As-T decreased when M. guillelmi was transferred to clean soil for 5 days. During the 42-day incubation in As30, the antioxidative responses including reactive oxygen species (ROS), glutathione (GSH) and glutathione-S-transferase (GST) were firstly increased and then decreased, and As-C (13.9-43.9 mg kg-1) kept higher than As-G (14.2-35.1 mg kg-1). Significantly positive correlations were found between As-T and GSH, As-C and GST. Moreover, tissue As after dissection (11.6-22.9 mg kg-1) was obviously lower than that after egestion (11.4-26.4 mg kg-1), but significantly related to ROS and GSH. Taken together, M. guillelmi exhibited excellent capacity of As efflux, and GSH explained tissue As accumulation while GST facilitated the As elimination via cast. Besides, dissection instead of egestion revealed the As efflux in M. guillelmi more accurately. These findings contributed to a better understanding of how geophagous earthworm M. guillelmi regulated tissue As accumulation for As stress tolerance, and recommended an optimal depuration mode to characterize As accumulation.

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Insecticides prevent or reduce insect crop damage, maintaining crop quality and quantity. Physiological traits, such as an insect's feeding behavior, influence the way insecticides are absorbed and processed in the body (toxicokinetics), which can be exploited to improve species selectivity. To fully understand the uptake of insecticides, it is essential to study their total uptake and toxicokinetics independent of their toxic effects on insects. We studied the toxicokinetics (TK) of insecticidally inactive test compounds incorporating agro-like structural motifs in larvae of the Egyptian cotton leafworm (Spodoptera littoralis, Lepidoptera), and their distribution across all biological matrices, using laboratory experiments and modeling. We measured Spodoptera larval behavior and temporal changes of whole-body concentrations of test compounds during feeding on treated soybean leaf disks and throughout a subsequent depuration period. Differences in the distribution of the total quantities of compounds were found between the biological matrices leaf, larva, and feces. Rate constants for uptake and elimination of test compounds were derived by calibrating a toxicokinetic model to the whole-body concentrations. Uptake and elimination rate constants depended on the physicochemical properties of the test compounds. Increasing hydrophobicity increased the bioaccumulation potential of test compounds. Incomplete quantities in larval matrices indicated that some compounds may undergo biotransformation. As fecal excretion was a major elimination pathway, the variable time of release and number of feces pellets led to a high variability in the body burden. We provide quantitative models to predict the toxicokinetics and bioaccumulation potential of inactive insecticide analogs (parent compounds) in Spodoptera.

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The quality of Pacific oyster (Crassostrea gigas) can be affected by many factors during depuration, in which temperature is the major element. In this study, we aim to determine the quality and plasmalogen changes in C. gigas depurated at different temperatures. The quality was significantly affected by temperature, represented by varying survival rate, glycogen content, total antioxidant capacity, alkaline phosphatase activity between control and stressed groups. Targeted MS analysis demonstrated that plasmalogen profile was significantly changed during depuration with PUFA-containing plasmalogen species being most affected by temperature. Proteomics analysis and gene expression assay further verified that plasmalogen metabolism is regulated by temperature, specifically, the plasmalogen synthesis enzyme EPT1 was significantly downregulated by high temperature and four plasmalogen-related genes (GPDH, PEDS, Pex11, and PLD1) were transcriptionally regulated. The positive correlations between the plasmalogen level and quality characteristics suggested plasmalogen could be regarded as a quality indicator of oysters during depuration.

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Microcystin-LR (MC-LR) is a hepatotoxic metabolite that naturally occurs during some cyanobacterial blooms in eutrophic waterbodies, and irrigation of edible plants with MC-LR-contaminated water causes bioaccumulation of the toxin. However, sufficient information about accumulation and depuration mechanics in hydroculture-grown herb plants is still lacking. This work aimed at 1) investigating bioaccumulation and depuration of MC-LR in basil, 2) verifying the possible MC-LR detoxification mechanisms in the plant, and 3) detecting the natural occurrence of MC-LR in basil (n = 50) collected from the Belgian market. Basil plants grown in a hydroculture were exposed to MC-LR (5, 20, and 50 µg L-1) spiked in a Hoagland solution for seven days. MC-LR depuration was also studied by transferring the plants to a non-contaminated Hoagland solution after exposure to MC-LR for another seven days. MC-LR concentrations in Hoagland solution, basil leaves, and roots were quantified using a validated UHPLC-MS/MS method. In addition, ELISA and LC-HRMS (only basil leaves) were used for confirmation. The results showed an increase in the accumulated levels of MC-LR at higher exposure doses, with higher MC-LR levels in roots than in leaves for all the treatment conditions. For MC-LR depuration, significant reductions were observed in all the treatment conditions for roots only. No MC-LR conjugates, potentially related to metabolism, were detected by LC-HRMS. Finally, MC-LR was detected in one store-bought basil sample, representing the first occurrence of cyanotoxins in an edible crop from Belgium.

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Domoic acid (DA) is a potent neurotoxin produced by diatoms of the genus Pseudo-nitzschia and is responsible for Amnesic Shellfish Poisoning (ASP) in humans. Some fishery resources of high commercial value, such as the king scallop Pecten maximus, are frequently exposed to toxic Pseudo-nitzschia blooms and are capable of accumulating high amounts of DA, retaining it for months or even a few years. This poses a serious threat to public health and a continuous economical risk due to fishing closures of this resource in the affected areas. Recently, it was hypothesized that trapping of DA within autophagosomic-vesicles could be one reason explaining the long retention of the remaining toxin in P. maximus digestive gland. To test this idea, we follow the kinetics of the subcellular localization of DA in the digestive glands of P. maximus during (a) the contamination process - with sequential samplings of scallops reared in the field during 234 days and naturally exposed to blooms of DA-producing Pseudo-nitzschia australis, and (b) the decontamination process - where highly contaminated scallops were collected after a natural bloom of toxic P. australis and subjected to DA-depuration in the laboratory for 60 days. In the digestive gland, DA-depuration rate (0.001 day-1) was much slower than contamination kinetics. The subcellular analyses revealed a direct implication of early autophagy in DA sequestration throughout contamination (r = 0.8, P < 0.05), while the presence of DA-labeled residual bodies (late autophagy) appeared to be strongly and significantly related to slow DA-depuration (r = -0.5) resembling an analogous DA-tattooing in the digestive glands of P. maximus. This work provides new evidence about the potential physiological mechanisms involved in the long retention of DA in P. maximus and represents the baseline to explore procedures to accelerate decontamination in this species.

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Crayfish rely on their chemosensory system for many essential behaviours including finding food, finding mates, and to recognize individuals. Copper can impair chemosensation in crayfish at low concentrations; however, it is not clear if the effect is ameliorated once copper is removed. To better understand the effect of and recovery from copper exposure in crayfish, we exposed Northern clearwater crayfish (Faxonius propinquus) to 31.3 [Formula: see text] copper for 24 h and measured the response of the crayfish to a food cue. The crayfish were then placed into clean water to depurate for an 24 h. The results demonstrated that the crayfish did not respond to a food cue if they had been exposed to copper, but showed a full response after a 24 h recovery period without copper. Higher concentrations of copper have shown a much longer-term effect in rusty crayfish (Faxonius rustics), indicating there is a concentration where the copper is causing longer-term damage instead of just impairing chemosensation. These results highlight the fact that even though contaminants like copper can have profound effects at low concentrations, by removing the contaminants the effect can be ameliorated.

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The global consumption of antibiotics leads to their possible occurrence in the environment. In this context, nature-based solutions (NBS) can be used to sustainably manage and restore natural and modified ecosystems. In this work, we studied the efficiency of the NBS free-water surface wetlands (FWSWs) using Eichhornia crassipes in microcosm for enrofloxacin removal. We also explored the behavior of enrofloxacin in the system, its accumulation and distribution in plant tissues, the detoxification mechanisms, and the possible effects on plant growth. Enrofloxacin was initially taken up by E. crassipes (first 100 h). Notably, it accumulated in the sediment at the end of the experimental time. Removal rates above 94% were obtained in systems with sediment and sediment + E. crassipes. In addition, enrofloxacin was found in leaves, petioles, and roots (8.8-23.6 µg, 11-78.3 µg, and 10.2-70.7 µg, respectively). Furthermore, enrofloxacin, the main degradation product (ciprofloxacin), and other degradation products were quantified in the tissues and chlorosis was observed on days 5 and 9. Finally, the degradation products of enrofloxacin were analyzed, and four possible metabolic pathways of enrofloxacin in E. crassipes were described.

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