RESUMEN

Here, we report the effect of DNA methylation on the velocity of DNA translocation through a nanochannel, as determined by measuring differences in translocation velocities between methylated and non-methylated DNA molecules. We found that the velocity of translocation of methylated DNA was faster than that of non-methylated DNA, which we attributed to variation in the coefficients of diffusion and friction with the nanochannel wall, due to the increased molecular weight and stiffness, respectively, of methylated DNA.

Asunto(s)

ADN/metabolismo , Nanotecnología/instrumentación , ADN/química , Metilación de ADN , Difusión , Peso Molecular