RESUMEN
Microplastics (MPs), which are widely dispersed in terrestrial environments, threaten crop growth and human food security. However, plant accumulation and phytotoxicity related to the size effects of MPs remain insufficiently explored. This study investigated the accumulation and toxicity of two sizes of MPs on Capsicum annuum Linn. (C. annuum) through fluorescence tracing and antioxidant defense system assessment. The results revealed that the size of MPs significantly impacts their accumulation characteristics in C. annuum roots, leading to variations in toxic mechanisms, including oxidative stress and damage. Smaller MPs and higher exposure concentrations result in more pronounced growth inhibition. C. annuum roots have a critical size threshold for the absorption of MPs of approximately 1.2 µm. MPs that enter the root tissue exhibit an aggregated form, with smaller-sized MPs displaying a greater degree of aggregation. MP exposure induces oxidative stress in root tissues, with high concentrations of smaller MPs causing lipid peroxidation. Analysis of the IBR values revealed that C. annuum roots utilize ascorbic acid (ASA) to prevent oxidative damage caused by larger MPs. Conversely, smaller MPs primarily induce superoxide dismutase (SOD) and glutathione (GSH). These results emphasize the significant impact of MP size on plant antioxidant defense response mechanisms, laying the foundation for further investigating the implications for human health.
Asunto(s)
Capsicum , Microplásticos , Estrés Oxidativo , Capsicum/metabolismo , Capsicum/crecimiento & desarrollo , Microplásticos/toxicidad , Raíces de Plantas/metabolismo , Contaminantes del Suelo/metabolismo , Contaminantes del Suelo/toxicidad , Peroxidación de Lípido , Antioxidantes/metabolismo , Superóxido Dismutasa/metabolismo , Glutatión/metabolismoRESUMEN
Cadmium (Cd) and sulfamethoxazole (SMX) frequently coexist in farmlands, yet their synergistic toxicological impacts on terrestrial invertebrates remain unexplored. In this study, earthworms were exposed to artificial soils percolated with Cd (5 mg/kg), SMX (5 mg/kg) or combination of them for 7 days, followed by a 12-day elimination phase in uncontaminated soil. The uptake of Cd and SMX by the earthworms, along with their subcellular distribution, was meticulously analyzed. Additionally, a suite of biomarkers-including superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA), and weight loss-were evaluated to assess the health status of the earthworms and the toxicological effects of the Cd and SMX mixture. Notably, the cotreatment with Cd and SMX resulted in a significantly higher weight loss in Eisenia fetida (41.25 %) compared to exposure to Cd alone (26.84 %). Moreover, the cotreatment group exhibited substantially higher concentrations of Cd in the total internal body, fraction C (cytosol), and fraction E (tissue fragments and cell membranes) in Eisenia fetida compared to Cd alone counterparts. The combined exposure also significantly elevated the SMX levels in the total body and fraction C compared with the SMX-only treated earthworms. Additionally, Eisenia fetida subjected to the combined treatment showed markedly increased activities of SOD, CAT, and MDA compared to those treated with Cd alone. The effect addition indices (EAIs), ranging from 1.00 to 2.23, unequivocally demonstrated a synergistic effect of the combined treatments. Interestingly, relocating the earthworms to clean soil did not mitigate the observed adverse effects. These findings underscore the increased risk posed by the Cd-SMX complex to terrestrial invertebrates in agricultural areas.
Asunto(s)
Biomarcadores , Cadmio , Oligoquetos , Contaminantes del Suelo , Sulfametoxazol , Oligoquetos/efectos de los fármacos , Oligoquetos/fisiología , Animales , Sulfametoxazol/toxicidad , Cadmio/toxicidad , Contaminantes del Suelo/toxicidad , Biomarcadores/metabolismo , Malondialdehído/metabolismo , Superóxido Dismutasa/metabolismo , Catalasa/metabolismoRESUMEN
The antidepressant effect of zinc on mammals has been documented in recent decades, and the concentration of the antidepressant fluoxetine (FLX) in aquatic environments has been rising constantly. The aim of the present study is to evaluate the combined toxicity of a serotonin reuptake inhibitor (FLX) and Zn2+ on a non-target aquatic model organism Daphnia magna. Animals were exposed to single and binary combinations of FLX (20.5 and 41 µg/L for subchronic and 41 and 82 µg/L for acute exposures) and Zn2+ (40 µg/L for subchronic and 80 µg/L for acute exposures). In vivo experiments were done for 7 days subchronic and 48 h acute exposure, while subcellular supernatants of whole Daphnia lysate (WDL) were directly treated with the same concentrations used in the acute experiments. Morphological characteristics, Ca2+-ATPase, antioxidant enzyme activities, and lipid peroxidation were examined. There was antioxidant system suppression and Ca2+-ATPase inhibition despite the diverse response patterns due to duration, concentration, and toxicant type. After acute exposure, biomarkers showed a diminishing trend compared to subchronic exposure. According to integrated biomarker response index (IBR) analysis, in vivo Zn2+ exposure was reasonably effective on the health of D. magna, whereas exposure of WDL to Zn2+ had a lesser impact. FLX toxicity increased in a concentration-dependent manner, reversed by the combined exposure. We concluded that potential pro-oxidative and adverse Ca2+-ATPase effects of FLX and Zn2+ in D. magna may also have harmful impact on ecosystem levels. Pharmaceutical exposure (FLX) should be considered along with their potential to interact with other toxicants in aquatic biota.
Asunto(s)
Biomarcadores , Daphnia magna , Fluoxetina , Contaminantes Químicos del Agua , Zinc , Animales , Antioxidantes , Biomarcadores/metabolismo , Daphnia magna/efectos de los fármacos , Fluoxetina/toxicidad , Peroxidación de Lípido/efectos de los fármacos , Contaminantes Químicos del Agua/toxicidadRESUMEN
Cadmium (Cd) and fenpyroximate are common soil contaminants found together in the field, but their combined toxicity to terrestrial invertebrates has not been studied. Therefore, earthworms Aporrectodea jassyensis and Eisenia fetida were exposed into Cd (5, 10, 50 and 100 µg/g) and fenpyroximate (0.1, 0.5, 1, and 1.5 µg/g) and their mixture, and multiple biomarker responses (mortality, catalase (CAT), superoxide dismutase (SOD), total antioxidant activity (TAC), lipid peroxidation (MDA), protein content, weight loss and subcellular partitioning) were determined to estimate health status and mixture effect. MDA, SOD, TAC, and weight loss were significantly correlated with Cd in total internal and debris (p < 0.01). Fenpyroximate altered the subcellular distribution of Cd. It appears that maintaining Cd in a non-toxic form was the earthworms' primary Cd detoxification strategy. CAT activity was inhibited by Cd, fenpyroximate, and their combined presence. BRI values for all treatments indicated a major and severe alteration in earthworm's health. The combined toxicity of Cd and fenpyroximate was greater than the toxicity of either substance alone. According to EAI, all combined treatments exhibited a clear antagonistic effect. In general, the sensitivity of A. jassyensis was greater than that of E. fetida.
Asunto(s)
Oligoquetos , Contaminantes del Suelo , Animales , Cadmio/análisis , Contaminantes del Suelo/análisis , Biomarcadores/metabolismo , Superóxido Dismutasa/metabolismo , SueloRESUMEN
The interactive effects of ocean acidification (OA) and copper (Cu) ions on the mussel Mytilus galloprovincialis are not well understood. The underlying mechanisms also remain obscure. In this study, individuals of M. galloprovincialis were exposed for 28 days to 25 µg/L and 50 µg/L Cu ions at two pH levels (ambient level - pH 8.1; acidified level - pH 7.6). The mussels were then monitored for 56 days to determine their recovery ability. Physiological parameters (clearance rate and respiration rate), oxidative stress and neurotoxicity biomarkers (activities of superoxide dismutase, lipid peroxidation, catalase, and acetylcholinesterase), as well as the recovery ability of these parameters, were investigated in two typical tissues (i.e., gills and digestive glands). Results showed that (1) OA affected the bioconcentration of Cu in the gills and digestive glands of the mussels; (2) both OA and Cu can lead to physiological disturbance, oxidative stress, cellular damage, energy metabolism disturbance, and neurotoxicity on M. galloprovincialis; (3) gill is more sensitive to OA and Cu than digestive gland; (4) Most of the biochemical and physiological alternations caused by Cu and OA exposures in M. galloprovincialis can be repaired by the recovery experiments; (5) integrated biomarker response (IBR) analysis demonstrated that both OA and Cu ions exposure caused survival stresses to the mussels, with the highest effect shown in the co-exposure treatment. This study highlights the necessity to include OA along with pollutants in future studies to better elucidate the risks of ecological perturbations. The work also sheds light on the recovery of marine animals after short-term environmental stresses when the natural environment has recovered.
Asunto(s)
Mytilus , Contaminantes Químicos del Agua , Acetilcolinesterasa/metabolismo , Animales , Biomarcadores/metabolismo , Catalasa/metabolismo , Cobre/análisis , Concentración de Iones de Hidrógeno , Iones/metabolismo , Estrés Oxidativo , Agua de Mar , Superóxido Dismutasa/metabolismo , Contaminantes Químicos del Agua/análisisRESUMEN
The aim of the present study is the first to evaluate the ecotoxic state of the marine environment in Anza-Taghazout coasts (Morocco) after installation of two wastewater treatment plants using a natural population of marine bivalves Mytilus galloprovincialis. These coasts are exposed to many discharges generating, thus, different sources of pollutants. These pollutants can modulate the physiological responses of marine bivalves to environmental stress. In this context, a multibiomarker approach consisting of a battery of biomarker evaluation was used to assess the response of these species to stress. In the whole soft tissues of M. galloprovincialis, four biomarkers were evaluated as follows: acetylcholinesterase (AChE), glutathione S-transferase (GST), catalase (Cat), and malondialdehyde activity (MDA). In parallel, physico-chemical parameters were measured in the marine water of Anza-Taghazout within three selected sites: S1 considered as "hotspot" located at Anza city; S2 located near of Aourir city; and the third site, S3 "reference" located in Imouran beach. Our results showed that activities of both glutathione S-transferase and catalase were higher in M. galloprovincialis collected from site S1, but high values of malondialdehyde and acetylcholinesterase activities were observed successively at S3 and S2. Application of integrated biomarker response (IBR) index was suitable for classifying the stress response in the M. galloprovincialis but did not allow to evaluate the level of the xenobiotic exposure in the studied sites. The statistical results did not show any significant differences between the three studied sites, and therefore, S1 has recently become clean due to the installation of two wastewater treatment plants.
Asunto(s)
Mytilus , Contaminantes Químicos del Agua , Purificación del Agua , Acetilcolinesterasa , Animales , Biomarcadores , Monitoreo del Ambiente , MalondialdehídoRESUMEN
Perfluorooctanoic acid (PFOA) and arsenic are ubiquitous environmental contaminants and could co-exist in soil. However, data on their possible combined toxic effects on terrestrial organisms are still lacking. In this study, we exposed earthworm Eisenia fetida to artificial soil spiked with different sub-lethal levels of PFOA, arsenite (As(III)) or their mixture for 28 days. The bioaccumulation and multi-biomarker responses in the earthworms were measured. Results showed that the co-existence of PFOA and As(III) in soil enhanced the bioaccumulation of arsenic while reduced the bioaccumulation of PFOA. Most selected biomarkers exhibited significant responses at higher exposure levels and indicated oxidative damages. Biomarker Response Index (BRI) was used to integrate the multi-biomarker responses and the results showed significant dose-effect relationships between biological health status and exposure levels. Moreover, variation analysis of multi-biomarkers and BRI proved that As(III) exhibited more toxicity than PFOA to the earthworms. Based on BRI results, Effect Addition Index (EAI) was calculated to evaluate the joint effects of the two toxicants. According to EAI, the joint toxicity of PFOA and As(III) was related to exposure concentration, changing from synergism to slight antagonism with the increase of exposure level. These results provide valuable toxicological information for the risk assessment of co-exposure to PFOA and arsenic in the soil environment. Moreover, this study proved that BRI is an effective tool to integrate multi-biomarker responses, and its combination with EAI provides a useful combined approach to evaluate the joint effects of mixed contamination systems.
Asunto(s)
Arsenitos , Oligoquetos , Contaminantes del Suelo , Animales , Arsenitos/toxicidad , Biomarcadores , Caprilatos , Fluorocarburos , Suelo , Contaminantes del Suelo/análisis , Contaminantes del Suelo/toxicidadRESUMEN
Poultry litter is widely applied as a fertilizer even though it is one of the main antibiotic sources to agricultural soils. Long-term sublethal effects (56 days) on the antioxidant system of Eisenia andrei earthworms following exposure to fluoroquinolone-contaminated poultry litter (enrofloxacin + ciprofloxacin) at 5.0, 10, and 20 g kg-1 were evaluated. The following biomarkers were assessed: superoxide dismutase (SOD), catalase (CAT), glutathione-S-transferase (GST), reduced glutathione (GSH), and a lipid peroxidation (LPO) proxy. Significant CAT and SOD increases, and a moderate positive correlation (ρ = 0.67, p < 0.05) between these enzymes was observed. Glutathione-S-transferase levels increased significantly at 10 g kg-1, while GSH exhibited a dose-dependent response at 5.0 mg kg-1 (4-106%), 10 mg kg-1 (28-330 %), and 20 mg kg-1 (45-472%). LPO levels exhibited a decreasing trend with increasing poultry litter concentrations of 8-170% (5.0 g kg-1), 7-104% (10 mg kg-1), and 3-6% (20 mg kg-1). A principal component analysis (PCA) highlighted increased SOD and CAT activities, possibly due to increased reactive oxygen species (ROS) concentrations. Biological health status assessments based on the biomarker response index indicate major alterations in the first month of exposure and becoming moderate in the second month. These findings indicate an antioxidant system attenuation trend. It is possible, however, that successive poultry litter applications may reduce the long-term recovery capacity of the evaluated biomarkers.
Asunto(s)
Oligoquetos , Contaminantes del Suelo , Animales , Antibacterianos/farmacología , Antioxidantes/metabolismo , Catalasa/metabolismo , Glutatión/metabolismo , Glutatión Transferasa/metabolismo , Estado de Salud , Peroxidación de Lípido , Oligoquetos/metabolismo , Estrés Oxidativo , Aves de Corral , Contaminantes del Suelo/análisis , Superóxido Dismutasa/metabolismoRESUMEN
Microplastic, a well-documented emerging contaminant, is widespread in aquatic environments resulting from the production and fragmentation of large plastics items. The knowledge about the chronic toxic effects and behavioral toxicity of microplastics, particularly on freshwater benthic macroinvertebrates, is limited. In this study, adult Asian clams (Corbicula fluminea) were exposed to gradient microplastic solutions for 42 days to evaluate behavioral toxicity and chronic biotoxicity. The results showed that microplastics caused behavior toxicity, oxidative stress, and tissue damage in high-concentration treatments. Siphoning, breathing, and excretion was significantly inhibited (p < 0.05) at high-concentration treatments, suggesting that high-concentration microplastics induced behavioral toxicity in C. fluminea. Malondialdehyde content, superoxide dismutase, catalase, and glutathione reductase activities were significantly enhanced (p < 0.05) and the acetylcholinesterase was significantly inhibited (p < 0.05) throughout the exposure period in high-concentration treatments. Enzymes associated with energy supply were significantly higher at high-concentration microplastics treatments on D7 and D21. However, they recovered to a normal level on D42. The instability of the enzymes indicated that high-concentration microplastics induced oxidative stress and disorder in neurotransmission and energy supply. The gills of C. fluminea in treatments underwent cilia degeneration, which indicated that microplastics caused tissue damage in the gills. The analysis of integrated biomarker response values revealed that high-concentration microplastics led to long-term effects on the health of C. fluminea. In conclusion, continuous exposure to microplastics (10 mg L-1) would damage physical behavior and the antioxidant system of C. fluminea.
Asunto(s)
Corbicula , Contaminantes Químicos del Agua , Acetilcolinesterasa , Animales , Microplásticos , Plásticos/toxicidad , Poliestirenos , Contaminantes Químicos del Agua/análisis , Contaminantes Químicos del Agua/toxicidadRESUMEN
The present study was aimed to evaluate the toxic effects of a commonly used synthetic pyrethroid, λ cyhalothrin on the common carp, Cyprinus carpio L. The results depicted that 96 h LC50 value of λ cyhalothrin to the fish was 1.48 µg l-1. During 45 days of chronic exposure a significant reduction (p < 0.05) in the RBC, hemoglobin, and hematocrit value of fish was observed in λ cyhalothrin treated fish. Blood glucose, cholesterol and creatinine levels increased significantly, while total protein and albumin were significantly decreased (p < 0.05) in the exposed fish. Moreover, alanine aminotransferase and aspartate aminotransferase levels in the blood also increased significantly (p < 0.05) in the treated fish. In gills and liver, glutathione S-transferase (GST) and glutathione peroxidase (GPx) and in liver GST exhibited a significant initial augmentation followed by a subsequent reduction while catalase (CAT), superoxide dismutase (SOD), and malondialdehyde (MDA) level increased markedly with incrementing concentrations of λ cyhalothrin in both the organs. Acetylcholinesterase (AchE) activity in both gills and liver decreased in exposed fish upon addition λ cyhalothrin. However, the hazardous effects of λ cyhalothrin on C. carpio were characterized and portrayed by the development of integrated biomarker response (IBR), and biomarker response index (BRI). GUTS-SD and IT modeling were implied for a better interpretation of the toxicity. These results indicate that exposure to λ cyhalothrin alters the survivability at the acute level and the activity of hematological, plasma biochemical as well as enzymological and stress parameters (in gills and liver) at the sublethal level in C. carpio.
Asunto(s)
Carpas/metabolismo , Nitrilos/toxicidad , Piretrinas/toxicidad , Acetilcolinesterasa/metabolismo , Animales , Antioxidantes/metabolismo , Biomarcadores/metabolismo , Carpas/sangre , Carpas/crecimiento & desarrollo , Catalasa/metabolismo , Exposición a Riesgos Ambientales , Branquias/efectos de los fármacos , Glutatión Peroxidasa/metabolismo , Glutatión Transferasa/metabolismo , Insecticidas/toxicidad , Estrés Oxidativo/efectos de los fármacos , Superóxido Dismutasa/metabolismoRESUMEN
Arsenate (As-V) is a ubiquitous contaminant in soil as a result of excessive use of veterinary drugs and pesticides, causing enormous environmental risks. Multiple biomarkers have been used to assess the ecotoxicity of arsenic, however, the mechanisms of toxicity remain unclear. This paper describes the exposure of the earthworm (Eisenia fetida) to natural soil with different As-V concentrations for 28 days, then biomarkers from oxidative stress and burrowing behavior were quantified to evaluate As-V stress. Dynamic changes in reactive oxygen species (ROS), lipid peroxidation (MDA), adenosine triphosphate (ATP) content and antioxidant enzymes activity (Gpx, SOD, CAT) implied two stages of intensified stress responses and physiological adaptability. The transcriptional expression and regulation of antioxidant enzymes showed different responses. The mRNA expression of sod1 was up-regulated, while that of cat showed no significant change. The related regulators, nuclear factor erythroid 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1), showed dose-dependent activation, suggesting antioxidant defense induced by Nrf2 signaling. The burrowing behavior after 14-day exposure indicated that As-V inhibited burrowing activity, especially the burrow length and maximum burrow depth. These multiple biomarkers were integrated using a biomarker response index (BRI) model, which showed significant dose-effect relationship especially on day 28, and suggested that ATP was a sensitive and representative biomarker. This study provided evidence that burrowing activity, Nrf2 and HO-1 were useful biomarkers warranting inclusion into the BRI model. Arsenic toxicity was comprehensively understood through redox homeostasis regulation, biochemical and behavioral changes, and these results suggested new strategies for soil pollutants diagnosis.
Asunto(s)
Oligoquetos , Contaminantes del Suelo , Animales , Arseniatos/toxicidad , Biomarcadores/metabolismo , Catalasa/metabolismo , Oligoquetos/metabolismo , Estrés Oxidativo , Contaminantes del Suelo/toxicidad , Superóxido Dismutasa/metabolismoRESUMEN
The Estuarine-Lagoon Complex of Iguape-Cananéia (ELCIC), a Marine Protected Area (MPA) in Brazil, was the focus of this study that aimed to relate external levels of exposure to contaminants to toxic effects on Gobioides broussonnetii fish. Different anthropogenic contaminants such as metals, polycyclic aromatic hydrocarbons (PAHs) and pharmaceuticals and personal care products (PPCPs) were analyzed in the sediments; and biochemical, histopathological and genotoxicity biomarkers evaluated in fish; in two different seasons at three sites of the estuarine region. Higher contamination of the sediments was observed near the main urban center (Iguape city - IG). Metal concentrations were considered low to moderate, while PAHs concentrations were considered low. The concentrations of PPCPs increased due to the anthropogenic presence and were higher near IG and the Cananéia Island (CI). Contributions from historical mining, agriculture, nautical activities, oil, sewage and waste disposal, biomass and fossil fuels combustion were identified. Higher concentrations of metals and PPCPs were observed during the cold-dry season, suggesting influences of the lower hydrodynamics during the season of lower precipitation. Higher PAHs concentrations occurred in the hot-rainy season, indicating influences of greater human presence in summer. In fish, biological responses followed the same spatial and seasonal pattern. More pronounced changes in antioxidant, biotransformation, histopathological and genotoxic biomarkers were observed in IG and CI. The multivariate analysis and the integrated biomarkers response index (IBR) also evidenced worse environmental conditions in these sites. This result can indicate a negative influence of anthropogenic activities on the contamination of sediments and on the biological responses of fish. This study presented the first ecotoxicological data for the species and suggested that these chronic exposures can cause adverse effects on this fish population. The data contribute to the understanding of local environmental quality and can be applied in the future to the environmental and social management of marine protected areas.
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Hidrocarburos Policíclicos Aromáticos , Contaminantes Químicos del Agua , Animales , Brasil , Ciudades , Monitoreo del Ambiente , Sedimentos Geológicos , Humanos , Hidrocarburos Policíclicos Aromáticos/análisis , Hidrocarburos Policíclicos Aromáticos/toxicidad , Contaminantes Químicos del Agua/análisis , Contaminantes Químicos del Agua/toxicidadRESUMEN
The outcomes of this research offer novel insights into the toxic effects of nanoparticles (i.e., nanoplastics or other nanomaterials) on the benthos. Herein, this study aimed to evaluate the accumulation pathway, distribution characteristics and potential biotoxicity of polystyrene nanoplastics in C. fluminea. The results revealed that nanoplastics could accumulate in the mantle through adherence, in the visceral mass through ingestion and in the gill through respiration. The gill, intestine and stomach were the main accumulation organs for nanoplastics. The aggregation of nanoplastics was observed in C. fluminea, which may exacerbate their biotoxicity. Moreover, oxidative stress was observed in the visceral mass, gill and mantle. Liver damage, neurotoxicity and intestinal inflammation were caused by imbalance in the antioxidation system. Analysis of IBR values showed that the visceral mass had a more effective response to oxidative stress than the gill and mantle after exposure to nanoplastics.
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Bivalvos/efectos de los fármacos , Microplásticos/toxicidad , Contaminantes Químicos del Agua/toxicidad , Animales , Bivalvos/metabolismo , Fluorescencia , Mucosa Gástrica/metabolismo , Branquias/metabolismo , Mucosa Intestinal/efectos de los fármacos , Mucosa Intestinal/metabolismo , Hígado/efectos de los fármacos , Hígado/patología , Síndromes de Neurotoxicidad/metabolismo , Síndromes de Neurotoxicidad/patología , Síndromes de Neurotoxicidad/veterinaria , Estrés Oxidativo/efectos de los fármacosRESUMEN
The presence of pharmaceuticals in the aquatic ecosystem has become a topic of growing interest in recent years. In this study, the marine clam Ruditapes philippinarum was exposed during 14 days to concentrations close to those found in the environment: (15 µg L-1) of carbamazepine (CBZ), diclofenac (DCF) and ibuprofen (IBU), three pharmaceuticals widely used worldwide and commonly found within the aquatic environment. Additionally, exposure was followed by a depuration phase (7 days). A battery of biomarkers (superoxide dismutase SOD, catalase CAT, glutathione reductase GR, total glutathione peroxidase T-GPx, glutathione transferase GST, lipid peroxidation LPO, acetylcholinesterase AChE and metallothionein MT) was evaluated throughout the exposure and depuration. The Integrated Biomarker Response index was calculated with all selected biomarkers and used as a complementary tool in the evaluation of the organisms' health status. Exposure induced changes in the clams' biochemical responses that led to the hypothesis of the harmful role of the pharmaceuticals resulting in negative effects (changes in enzyme activities, LPO and MT levels, related to ROS production) particularly after short-term exposure. However, the clams showed the ability to cope with these imbalances by recovering their general oxidative status by the end of exposure.
Asunto(s)
Biomarcadores/metabolismo , Bivalvos/efectos de los fármacos , Carbamazepina/toxicidad , Diclofenaco/toxicidad , Ibuprofeno/toxicidad , Acetilcolinesterasa/metabolismo , Animales , Branquias/efectos de los fármacos , Branquias/metabolismo , Humanos , Peroxidación de Lípido/efectos de los fármacos , Metalotioneína/metabolismo , Estrés Oxidativo/efectos de los fármacos , Contaminantes Químicos del Agua/toxicidadRESUMEN
This study combined data of the concentrations of metals, polycyclic aromatic hydrocarbons (PAHs) and pharmaceuticals and personal hygiene products (PPCPs) in the sediments and the biological responses of the Atherinella brasiliensis fish in two different sites and climate seasons in the Estuarine-Lagoon Complex of Iguape-Cananéia, Southeast Brazil. The presence of metals, PAHs, and PPCPs were observed in the sediments demonstrating the contamination throughout the system with contributions of sewage and residues disposal, oil and combustion of biomass and fossil fuels. Higher contaminations were identified in the point of greater human presence (C - Cananéia City), especially during the cold-dry season. The influence of anthropic activities and variations in the estuarine conditions, such as lower hydrodynamics during the lower rainfall period, were observed. In fish, spatial and seasonal changes in the parameters of oxidative stress and biotransformation, genotoxicity and histopathological alterations followed the same trend, with more pronounced responses in C in the cold-dry season. The biological responses of the fish revealed adverse effects in the local species population and indicated the presence of metals, PAHs and PPCPs as stressors. The multivariate analysis and the integrated biomarker response index (IBR) corroborated with these results, also indicating that site C had the worst environmental quality. The present study provides new information about the contamination of the sediments of Estuarine-Lagoon Complex of Iguape-Cananéia and the chronic exposure to contaminants in A. brasiliensis. Therefore, contributing to a better understanding of the local environmental quality with data that can support protective management of the area.
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Monitoreo del Ambiente/métodos , Estuarios , Peces/metabolismo , Sedimentos Geológicos/química , Contaminantes Químicos del Agua/análisis , Animales , Brasil , Ciudades , Contaminantes Ambientales/análisis , Humanos , Estaciones del Año , Especies CentinelaRESUMEN
Agrochemicals and heavy metals are widespread contaminants in urban soil and could co-exist as mixture, which could cause unexpected risk to terrestrial organism. To assess the joint effect of herbicide Siduron and Cd, a battery of sub-lethal biomarkers was studied using earthworm ecotoxicological assay. Most selected biomarkers appeared significant but complicated responses with the increasing concentration of contaminants after 28-day exposure. In order to quantify the overall effect of the mixture contaminants, Biomarker Response Index (BRI) was used to integrate the multiple responses. Concentration Addition Index (CAI) and Effect Addition Index (EAI) were introduced to assess types of joint effect. Results showed significantly dose-effect responses between BRI and contaminant exposure concentrations. Integrated toxicity increased obviously under joint treatments of Siduron and Cd compared to their individual treatments. According to CAI, a clear antagonism was observed at relatively lower effects and gradually transformed to slight synergism with an increase of effects, while EAI showed the joint effect of addition at the whole range of effect levels. Thus, compared to the simple analysis of those complicated responses, BRI is an effective method to determine the integrated toxicity of mixture and its combination with joint effect indices (CAI and EAI) provides more worthy risk assessment on toxicity interaction among compounds.