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Increased awareness of the impact of human activities on the environment has emerged in recent decades. One significant global environmental and human health issue is the development of materials that could potentially have negative effects. These materials can accumulate in the environment, infiltrate organisms, and move up the food chain, causing toxic effects at various levels. Therefore, it is crucial to assess materials comprising nano-scale particles due to the rapid expansion of nanotechnology. The aquatic environment, particularly vulnerable to waste pollution, demands attention. This review provides an overview of the behavior and fate of metallic nanoparticles (NPs) in the aquatic environment. It focuses on recent studies investigating the toxicity of different metallic NPs on aquatic organisms, with a specific emphasis on thiol-biomarkers of oxidative stress such as glutathione, thiol- and related-enzymes, and metallothionein. Additionally, the selection of suitable measurement methods for monitoring thiol-biomarkers in NPs' ecotoxicity assessments is discussed. The review also describes the analytical techniques employed for determining levels of oxidative stress biomarkers.

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N-1,3-Dimethylbutyl-N'-phenyl-p-quinone diamine (6PPDQ) is a derivative of 6PPD, a synthetic antioxidant used in tire manufacturing to control the degradation caused by oxidation and heat aging. Its discovery in 2020 has raised important environmental concern, particularly regarding its association with acute mortality in coho salmon, prompting surge in research on its occurrence, fate, and transport in aquatic ecosystems. Despite this attention, there remain notable gaps in grasping the knowledge, demanding an in depth overview. Thus, this review consolidates recent studies to offer a thorough investigation of 6PPDQ's environmental dynamics, pathways into aquatic ecosystems, toxicity to aquatic organisms, and human health implications. Various aquatic species exhibit differential susceptibility to 6PPDQ toxicity, manifesting in acute mortalities, disruption of metabolic pathways, oxidative stress, behavioral responses, and developmental abnormalities. Whereas, understanding the species-specific responses, molecular mechanisms, and broader ecological implications requires further investigation across disciplines such as ecotoxicology, molecular biology, and environmental chemistry. Integration of findings emphasizes the complexity of 6PPDQ toxicity and its potential risks to human health. However, urgent priorities should be given to the measures like long-term monitoring studies to evaluate the chronic effects on aquatic ecosystems and the establishment of standardized toxicity testing protocols to ensure the result comparability and reproducibility. This review serves as a vital resource for researchers, policymakers, and environmental professionals seeking appraisals into the impacts of 6PPDQ contamination on aquatic ecosystems and human health.

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Mercury is a hazardous heavy metal that is distributed worldwide in aquatic ecosystems. Methylmercury (MeHg) poses significant toxicity risks to aquatic organisms, primarily through bioaccumulation and biomagnification, due to its strong affinity for protein thiol groups, which results in negative effects even at low concentrations. MeHg exposure can cause various physiological changes, oxidative stress, neurotoxicity, metabolic disorders, genetic damage, and immunotoxicity. To assess the risks of MeHg contamination in actual aquatic ecosystems, it is important to understand how MeHg interacts with environmental factors such as temperature, pH, dissolved organic matter, salinity, and other pollutants such as microplastics and organic compounds. Complex environmental conditions can cause potential toxicity, such as synergistic, antagonistic, and unchanged effects, of MeHg in aquatic organisms. This review focuses on demonstrating the toxic effects of single MeHg exposure and the interactive relationships between MeHg and surrounding environmental factors or pollutants on aquatic organisms. Our review also recommends further research on biological and molecular responses in aquatic organisms to better understand the potential toxicity of combinational exposure.

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As international regulations on aquatic organism discharge in ships' ballast water take effect, port state authorities will need a sampling device for compliance inspectors to potentially use for sample collection. Importantly, the device must be easily operable, quickly connectable to the main ballast water pipe, and reliable in collecting a representative sample of the ballast water flowing through the pipe. Existing sampling devices currently fall short in these aspects. Therefore, we've developed, manufactured, and validated a new sampling skid. This design enables the regulation of flow velocity and flow rate, facilitating the simultaneous, convenient collection of organisms ≥50 µm and other biological samples. In this study, we conducted a comparative analysis of sampling outcomes between traditional net sampling devices, using land-based experiments to assess the accuracy and precision of the innovative sampling skid. The findings show that, despite significant differences in <10 µm organism concentrations, the sampling skid, as an alternative to traditional net and other skid devices, has demonstrated substantial potential and feasibility.

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The wide occurrence of micro- and nanoplastics (MPs/NPs) within aquatic ecosystems has raised increasing concerns regarding their potential effects on aquatic organisms. However, the effects of MPs/NPs on intestinal health and microbiota of fish remain controversial, and there is a lack of comprehensive understanding regarding how the impact of MPs/NPs is influenced by MPs/NPs characteristics and experimental designs. Here, we conducted a global analysis to synthesize the effects of MPs/NPs on 47 variables associated with fish intestinal health and microbiota from 118 studies. We found that MPs/NPs generally exerted obvious adverse effects on intestinal histological structure, permeability, digestive function, immune and oxidative-antioxidative systems. By contrast, MPs/NPs showed slight effects on intestinal microbial variables. Further, we observed that the responses of intestinal variables to MPs/NPs were significantly regulated by MPs/NPs characteristics and experimental designs. For instance, polyvinyl chloride plastics showed higher toxicity to fish gut than polyethylene and polystyrene did. Additionally, larval fish appeared to be more sensitive to MPs/NPs than juvenile fish. Collectively, this study highlights the potential impacts of MPs/NPs on intestinal health and microbiota of fish, and underscores the determinant role of MPs/NPs characteristics and experimental designs in MPs/NPs toxicity.

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Dibutyl phthalate (DBP) is a commonly used plasticizer that is frequently detected in water samples due to its widespread use. Titanium dioxide nanoparticles (n-TiO2) have been found to enhance the harmful effects of organic contaminants by increasing their bioavailability in aquatic environments. However, the combined toxic effects of DBP and n-TiO2 on aquatic organisms remain unclear. This study aimed to investigate the neurotoxicity of DBP and n-TiO2 synergistic exposure during the early life stage of zebrafish. The results of the study revealed that co-exposure of DBP and n-TiO2 led to an increase in deformities and a significant reduction in the active duration of zebrafish larvae. Furthermore, the co-exposure of DBP and n-TiO2 resulted in elevated levels of oxidative stress and altered gene expression related to neurodevelopment and apoptosis. Notably, n-TiO2 exacerbated the oxidative damage and apoptosis induced by DBP alone exposure. Additionally, co-exposure of the 1.0 mg/L DBP and n-TiO2 significantly affected the expression of genes associated with neurodevelopment. Moreover, disturbances in amino acid metabolism and interference with lipid metabolism were observed as a result of DBP and n-TiO2 co-exposure. In general, n-TiO2 aggravated the neurotoxicity of DBP in the early life stage of zebrafish by increasing oxidative stress, apoptosis, and disrupting amino acid synthesis and lipid metabolism. Therefore, it is essential to consider the potential risks caused by DBP and nanomaterials co-existence in the aquatic environment.

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BACKGROUND: Al exists naturally in the environment and is an important component in acidic soils, which harm almost all plants. Furthermore, Al is widely used in food additives, cosmetics, and medicines, resulting in living organisms ingesting traces of Al orally or dermally every day. Accordingly, Al accumulates in the body, which can cause negative bioeffects and diseases, and this concern is gaining increasing attention. Therefore, to detect and track Al in the environment and in living organisms, the development of novel Al-selective probes that are water-soluble and exhibit fluorescence at long wavelengths is necessary. RESULTS: In this study, an Al3+-selective fluorescent probe PSP based on a novel pyrone molecule was synthesized and characterized to detect and track Al in biological systems. PSP exhibited fluorescence enhancement at 580 nm in the presence of Al3+ in aqueous media. Binding analysis using Job's plot and structural analysis using 1H NMR showed that PSP formed a 1:1 complex with Al3+ at the two carbonyl groups of the dimethyl malonate of the pyrone ring. Upon testing in biological systems, PSP showed good cell membrane permeability, detected intracellular Al3+ in human breast cancer cells (MDA-MB-231), and successfully imaged accumulated Al3+ in Microcystis aeruginosa and the larvae of Rheocricotopus species. SIGNIFICANCE: The novel Al3+-selective fluorescent probe PSP is highly effective and is expected to aid in elucidating the role of Al3+ in the environment and living organisms.

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Microplastics (MPs) are emerging ubiquitous pollutants in aquatic environment and have received extensive global attention. In addition to the traditional studies related to the toxicity of MPs and their carrier effects, their unique surface-induced biofilm formation also increases the ecotoxicity potential of MPs from multiple perspectives. In this review, the ecological risks of MPs biofilms were summarized and assessed in detail from several aspects, including the formation and factors affecting the development of MPs biofilms, the selective enrichment and propagation mechanisms of current pollution status of antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs) in MPs biofilms, the dominant bacterial communities in MPs biofilms, as well as the potential risks of ARGs and MGEs transferring from MPs biofilms to aquatic organisms. On this basis, this paper also put forward the inadequacy and prospects of the current research and revealed that the MGEs-mediated ARG propagation on MPs under actual environmental conditions and the ecological risk of the transmission of ARGs and MGEs to aquatic organisms and human beings are hot spots for future research. Relevant research from the perspective of MPs biofilm should be carried out as soon as possible to provide support for the ecological pollution prevention and control of MPs.

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Mercury (Hg), especially methylmercury (MeHg), which is highly neurotoxic, is a global pollutant that can affect human health because of its accumulation in aquatic products. Poyang Lake, an inland lake in China, has been significantly affected by human activity, yet there is limited understanding of local mercury contamination and potential exposure pathways to humans. In this study, we explored the risks of mercury exposure by sampling sediments, plants, and aquatic organisms in the lake and surrounding areas and analyzing total Hg (THg) and MeHg levels. Sediment sampling was conducted at the main lake, rivers, rice paddies, and fishponds. Two dominant species of plants and 15 species of aquatic organisms were sampled and analyzed. We assessed the characteristics of mercury in sediments using the geo-accumulation index (Igeo), mercury exposure using the biomagnification factor (BMF) and biota sediment accumulation factor (BSAF), and risks using thresholds for adverse effects. The highest THg concentrations (137.04 ± 44.3 ng g-1 dw) were detected in the main lake sediments, whereas the highest MeHg concentrations (0.47 ± 0.6 ng g-1 dw) were detected in fishpond sediments. Mercury accumulation in the main lake sediments could be assessed as contaminated (Igeo > 0: 81.6%). Yellow catfish had the highest mercury concentration (THg 770.69 ± 199.7 ng g-1 dw; MeHg 741.93 ± 168.8 ng g-1 dw). Piscivores were adversely affected by carnivorous fish (50.8%), but all fish concentrations did not exceed the food safety standards recommend by China and the WHO. The mercury exposure results revealed significant Hg biomagnification and enrichment (BMF >1: 94.55%; BSAFmax = 1218). Long-term monitoring of aquatic organisms is warranted.

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A sensitive, robust, and highly efficient analytical methodology involving solid phase extraction coupled to ultra-high performance liquid chromatography tandem mass spectrometry was successfully established to detect 13 monoalkyl phthalate esters (MPAEs) in aquatic organisms and seawater. After the organisms were preprocessed using enzymatic deconjugation with ß-glucuronidase, extraction, purification, and qualitative and quantitative optimization procedures were performed. Under optimal conditions, the limits of detection varied from 0.07 to 0.88 µg/kg (wet weight) and 0.04-1.96 ng/L in organisms and seawater, respectively. Collectively, MPAEs achieved acceptable recovery values (91.0-102.7%) with relative standard deviations less than 10.4% and matrix effects ranging from 0.93 to 1.07 in the above matrix. Furthermore, MPAEs and phthalate esters were detected by the developed methodology and gas chromatography-triple quadrupole tandem mass spectrometer in practical samples, respectively. Mono-n-butyl phthalate and mono-iso-butyl phthalate were the most predominant congeners, accounting for 24.8-35.2% in aquatic organisms and seawater. Comprehensive health and ecological risks were higher after the MPAEs were incorporated than when phthalate esters were considered separately, and greater than their risk threshold. Therefore, the risks caused by substances and their metabolites in multiple media, with analogous structure-activity relationships, should be considered to ensure the safety of aquatic organisms and consumers.

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Emerging contaminants (ECs) are gaining global attention owing to their widespread presence and adverse effects on human health. ECs comprise numerous composite types and pose a potential threat to the growth and functional traits of species and ecosystems. Although the occurrence and fate of ECs has been extensively studied, little is known about their long-term biological effects. This review attempts to gain insights into the unhindered connections and overlaps in aquatic ecosystems. Microplastics (MPs), one of the most representative ECs, are carriers of other pollutants because of their strong adsorption capacity. They form a complex of pollutants that can be transmitted to aquatic organisms and humans through the extended food chain, increasing the concentration of pollutants by tens of thousands of times. Adsorption, interaction and transport effects of emerging contaminants in the aquatic environment are also discussed. Furthermore, the current state of knowledge on the ecotoxicity of single- and two-pollutant models is presented. Herein, we discuss how aquatic organisms within complex food networks may be particularly vulnerable to harm from ECs in the presence of perturbations. This review provides an advanced understanding of the interactions and potential toxic effects of ECs on aquatic organisms.

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Polycyclic aromatic hydrocarbons (PAHs) residues in fish, shrimp and shellfish have attracted attention because they are major species in the aquatic food chain and an important food source for humans. These organisms have various feeding habits and different living environments, and through the food chain, they can directly or indirectly connect particulate organic matter and human consumption. However, little attention has been paid to the bioaccumulation of PAHs in aquatic organism groups representing varied conditions and feeding habits in the food chain. In this study, 17 species of aquatic organisms, comprising fish, shrimp, and shellfish, were captured from 15 locations distributed within the river network of the Pearl River Delta. The concentration of 16 PAHs was measured in the aquatic organisms. The sum of the 16 measured PAHs ranged from 57.39 to 696.07 ng/g, dry weight, while phenanthrene had the highest individual content. The linear mixed effect model was applied to estimate the random effects of PAH accumulation in aquatic organisms. The result showed that the contributed proportion of variance to feeding habits (58.1 %) was higher than that of geographic distribution (11.8 %). In addition, one-way analysis of variance (ANOVA) demonstrated that the concentrations of PAHs depended on the water layer inhabited by the organism and its species status. Specifically, shellfish and carnivorous bottom-dwelling fish showed significantly higher levels than other aquatic organisms.

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Tire wear particles (TWP) have become the major microplastic pollution in China. Road runoff containing TWP leachate can decrease the eye size and even induced mortality in the aquatic organisms. However, the toxic mechanism of TWP and road particles (RP) leachate on aquatic organisms is still unclear. In this study, the zebrafish embryos were exposed to TWP or RP leachate for 5 days at both environmental relevant and high concentrations. The adverse outcome pathways (AOPs) were screened from individual to molecular levels. The morphological and behavioral analysis demonstrated that the leachate exposure mainly impaired the eye development of zebrafish larvae and inhibited the larval swim behavior and phototactic response, which are the adverse outcomes. The phototransduction modulated by zebrafish retina was significantly down-regulated through transcriptomics and metabolomics analysis. The eye histopathological analysis showed that the decreased thickness of the retinal outer nuclear layer (ONL) and retinal pigmented epithelium (RPE) after leachate exposure were caused by the decreased photoreceptor cells. Moreover, the expression of NR2E3 and TPO genes showed concentration-dependent down-regulation after leachate exposure. The inhibition of photoreceptor cell proliferation was identified as the main reason for photoreceptor cell decrease in zebrafish larval eye. This study, for the first time, uncovered the underlying toxic mechanism of TWP and RP on zebrafish larval eyes.

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Sunscreens are an important means of protection against sunburns, dyspigmentation, photoaging, and photocarcinogenesis. Sunscreens come in a variety of formulations that can protect against ultraviolet B (UVB) radiation, both UVB and ultraviolet A (UVA) radiation (broad-spectrum sunscreens), and UVB, UVA, and visible light (tinted broad-spectrum sunscreens). In the USA, there is currently a paucity of FDA-approved broad-spectrum filters on the market. Studies have identified the presence of multiple UV filters in water sources globally. Many laboratory studies have implicated the potential impact of UV filters on coral reef bleaching, the food chain, and human health. However, many of these studies are performed at concentrations that are much higher than those present in the natural environment. With increasing discussion surrounding the role of organic and inorganic UV filters as potential environmental pollutants over the past decade, approval of additional broad-spectrum filters would be an important means of alleviating the use of more controversial filters. The aim of this article is to review the effects of UV filters on health and the environment and explore potential adjunctive agents for photoprotection.

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A scientometric analysis was conducted to investigate the trends and development of crayfish research in terms of literature published, author, affiliation, and countries' collaborative networks, as well as the co-citation dataset (e.g., author, article, and keywords). The study analyzed 12,039 bibliographic datasets from the Web of Science, using CiteSpace as a tool for the co-citation analysis. The study revealed extraordinary increases in publication trends, with a total of 21,329 authors involved in approximately 80% of countries around the world (163/195) having conducted crayfish research. Unsurprisingly, countries such as the USA and China, followed by European countries, were among the top countries that have published crayfish-related studies. The findings also indicated that "invasive crayfish" was the world's top keyword for crayfish research. Crayfish species are important for both environmental sustainability (invasiveness and species composition) and social wellbeing (aquaculture), which provides directions for research, philanthropic, academic, government, and non-government organizations regarding how to invest limited resources into policies, programs, and research towards the future management of this species. Our study concluded that strategic collaboration among authors, institutions, and countries would be vital to tackle the issue of invasive crayfish species around the world.

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Microplastics (MPs), an emerging group of pollutants, not only have direct toxic effects on aquatic organisms but also cause combined toxicity by absorbing other pollutants. Triphenyltin (TPT), one of the most widely used organotin compounds, has adverse effects on aquatic organisms. However, little is known about the combined toxicity of MPs and TPT to aquatic organisms. To investigate the individual and combined toxicity of MPs and TPT, we selected the common carp (Cyprinus carpio) for a 42-day exposure experiment. Based on the environmental concentrations in a heavily polluted area, the experimental concentrations of MPs and TPT were set at 0.5 mg L-1 and 1 µg L-1, respectively. The effects of MPs combined with TPT on the carp gut-brain axis were evaluated by detecting gut physiology and biochemical parameters, gut microbial 16S rRNA, and brain transcriptome sequencing. Our results suggest that a single TPT caused lipid metabolism disorder and a single MP induced immunosuppression in carp. When MPs were combined with TPT, the involvement of TPT amplified the immunotoxic effect induced by MPs. In this study, we also explored the gut-brain axis relationship of carp immunosuppression, providing new insights for assessing the combined toxicity of MPs and TPT. At the same time, our study provides a theoretical basis for evaluating the coexistence risk of MPs and TPT in the aquatic environment.

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The use of facial masks has increased and is therefore being recognized as a large source of environmental microplastics. Herein, we naturally aged disposable masks in a lake for eight weeks and compared the toxicity of mask-derived microplastics depending on the aging process using zebrafish (Danio rerio). Zebrafish were exposed to virgin and aged mask fragments (VF and AF, respectively) for eight weeks. The aging process induced cracks on the surface of mask fragments and chemical adsorption. Both VF and AFs damaged the zebrafish's liver, gills, and intestine and adversely affected their digestive ability, and their movement-aggression was decreased. These observations highlight the consequences of indiscriminately discarding masks or AFs following consumption. In conclusion, personal protective equipment waste in the environment should be appropriately managed to prevent negative impacts on aquatic organisms and, consequently, on humans via the food chain.

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Effluent-fed streams, which receive inputs from wastewater treatment plants, are becoming increasingly common across the globe as urbanization intensifies. In semi-arid and arid regions, where many natural streams have dried up due to over extraction of water, many streams rely completely on treated effluent to sustain baseflow during dry seasons. These systems are often thought of as 'second-class' or highly disturbed stream ecosystems, but they have the potential to serve as refuges for native aquatic biota if water quality is high, especially in areas where few natural habitats remain. In this study, we investigated seasonal and longitudinal water quality dynamics at multiple sites across six reaches of three effluent-dependent rivers in Arizona (USA) with the objective (1) to quantify changes in effluent water quality due to distance traveled and season/climate and (2) to qualify whether water quality conditions in these systems are sufficient to support native aquatic species. Study reaches ranged in length from 3 to 31 km and in geographic setting from low desert to montane conifer forest. We observed the lowest water quality conditions (e.g., elevated temperature and low dissolved oxygen) during the summer in low desert reaches, and significantly greater natural remediation of water quality in longer vs. shorter reaches for several factors, including temperature, dissolved oxygen and ammonia. Nearly all sites met or exceeded water quality conditions needed to support robust assemblages of native species across multiple seasons. However, our results also indicated that temperature (max 34.2 °C), oxygen levels (min 2.7 mg/L) and ammonia concentrations (max 5.36 mg/L N) may occasionally be stressful for sensitive taxa at sites closest to effluent outfalls. Water quality conditions may be a concern during the summer. Overall, effluent-dependent streams have the capacity to serve as refuges for native biota in Arizona, and they may become the only aquatic habitat available in many urbanizing arid and semi-arid regions.

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Microplastics (MPs, diameter <5 mm) are widely distributed on Earth, especially in the oceans. Diatoms account for ∼40% of marine primary productivity and affect the global biogeochemical cycles of macroelements. However, the effects of MPs on marine nitrogen cycling remain poorly understood, particularly comparisons between nitrogen-replete and nitrogen-limited conditions. We found that MPs trigger the Matthew effect on nitrogen assimilation in diatoms, where MPs inhibited nitrogen assimilation under nitrogen-limited conditions while enhancing nitrogen metabolism under nitrogen-replete conditions in Phaeodactylum tricornutum. Nitrate reductase (NR) and nitrite reductase (NIR) are upregulated, but nitrate transporter (NRT) and glutamine synthetase (GS) are downregulated by MPs under nitrogen-limited conditions. In contrast, NR, NIR, and GS are all upregulated by MPs under nitrogen-replete conditions. MPs accelerate nitrogen anabolic processes with an increase in the accumulation of carbohydrates by 80.7 ± 7.9% and enhance the activities of key nitrogen-metabolizing enzymes (8.20-44.90%) under nitrogen-replete conditions. In contrast, the abundance of carbohydrates decreases by 22.0-34.4%, and NRT activity is inhibited by 79.0-86.5% in nitrogen-limited algae exposed to MPs. Metabolomic and transcriptomic analyses were performed to further explore the molecular mechanisms of reprogrammed nitrogen assimilation, including carbon metabolism, nitrogen transport and ammonia assimilation. The aforementioned spatial redistribution (e.g., the Matthew effect between nitrogen-replete and -limited conditions) of nitrogen assimilation highlights the potential risks of MP contamination in the ocean.

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The available information about whether wastewater-derived disinfection byproducts (DBPs) could elicit potential endocrine-related detrimental effects on aquatic organisms was scarce. Herein, the potential disrupting effects and underlying binding mechanism of 14 wastewater-derived aliphatic and aromatic DBPs and 12 other substances on Chinese rare minnow (Gobiocypris rarus) transthyretin (CrmTTR) were tested and revealed by in vitro and in silico methods. The amino acid sequences of CrmTTR were determined, and the recombinant CrmTTR with a molecular mass of 66.3 kDa was expressed and purified. In vitro assay results indicated that eight selected aromatic DBPs exhibited detectable CrmTTR disrupting ability. Meanwhile, six aliphatic DBPs were not CrmTTR binders. Molecular modeling results implied that hydrophobic hydrogen bonds and/or ionic pair interactions were non-negligible. Four binary classification models with high classification performance were constructed. A significant positive linear relationship was observed for the binding affinity data from CrmTTR and human TTR (n = 18, r = 0.922, p < 0.0001). However, the binding affinity for 13 out of 18 tested compounds with CrmTTR was higher than that with human TTR. All the results highlighted that some wastewater-derived DBPs may be potential disruptors on the aquatic organism endocrine system, and interspecies variation should not be neglected in future determination of the potential endocrine disrupting effects of wastewater-derived DBPs.

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