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To further clarify the impact of different rootstocks in grafted blueberry, fruit quality, mineral contents, and leaf gas exchange were investigated in 'O'Neal' blueberry (Vaccinium corymbosum) grafted onto 'Anna' (V. corymbosum) (AO), 'Sharpblue' (V. corymbosum) (SO), 'Baldwin' (V. virgatum) (BO), 'Plolific' (V. virgatum) (PO), and 'Tifblue' (V. virgatum) (TO) rootstocks and own-rooted 'O'Neal' (NO), and differences in anatomic structures and drought resistance were determined in AO, TO, and NO. The findings revealed that fruit quality in TO and PO was excellent, that of BO and SO was good, and that of AO and NO was medium. 'Tifblue' and 'Plolific' rootstocks significantly increased the levels of leaf phosphorus and net photosynthetic rate of 'O'Neal', accompanied by a synchronous increase in their transpiration rates, stomatal conductance, and intercellular CO2. Additionally, the comprehensive evaluation scores from a principal component analysis based on anatomic structure traits from high to low were in the order TO > AO > NO. The P50 (xylem water potential at 50% loss of hydraulic conductivity) values of these grafted plants descended in the order NO > AO > TO, and the branch hydraulic conductivity of TO and sapwood hydraulic conductivity of TO and AO were significantly lower than those of NO. Thus, TO plants exhibited the strongest drought resistance, followed by AO, and NO, and this trait was related to the effects of different rootstocks on the fruit quality of 'O'Neal' blueberry. These results provided a basis for a deeper understanding of the interaction between rootstocks and scions, as well mechanisms to improve blueberry fruit quality.

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Drought stress is also one of the important abiotic factors limiting plant growth and development, and the global temperature is rising year by year, resulting in a dry environment in most terrestrial forests, which will continue to affect the growth, development and reproduction of tree species in forests. European birch(Betula pendula Roth.) native to Europe, introduced to the mountains of eastern Liaoning in 1981 (annual precipitation of about 800mm), European birch relative to downy birch (B. pubescens)has strong adaptability and drought tolerance and cold tolerance, can grow normally in eastern Liaoning, but it is easy to be affected by drought at the seedling stage and cause death, many arid and semi-arid areas have no introduction and practical application of European birch, and there is less research on the drought resistance of European birch. This study used different concentrations of PEG-6000 treatment to simulate drought stress and clarify the changes of various growth physiological parameters and photosynthetic characteristics of European birch seedlings under drought stress, in order to investigate the physiological response mechanism of European birch under drought stress . This study used different concentrations of PEG-6000 treatment to simulate drought stress and clarify the changes of various growth physiological parameters and photosynthetic characteristics of European birch seedlings under drought stress, in order to investigate the physiological response mechanism of European birch under drought stress. The findings demonstrated that stress duration and increasing PEG concentration had a highly significant impact on the growth traits of European birch seedlings (p<0.01); With increasing stress concentration and stress time, antioxidant enzyme activity, membrane lipid peroxidation, and osmoregulatory substance concentrations increased significantly (p<0.01); With increasing stress concentration and duration, photosynthetic parameters and pigments decreased highly significantly (p<0.01); Under different PEG concentration treatments, the anatomical structure of seedling leaves changed more noticeably; there was a significant effect (p <0.05) on the change in mean stomatal length and a highly significant effect (p<0.01) on the change in mean stomatal structure. The study's findings serve as a foundation for the selection and breeding of new drought-tolerant European birch species, as well as a theoretical underpinning for the use of this species in landscaping and the promotion of new drought-tolerant species in China.

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Taxodium ascendens is a typical tree species with high flood tolerance, and it can generate knee roots in the wetlands. This study investigated the number and size of knee roots and the soil flooding conditions. Furthermore, we also measured physiology, biochemical responses, and the anatomical structure of knee roots and underground roots at different developmental stages. This study aimed to understand the adaptation mechanism of T. ascendens to flooding stress and the formation mechanism of the knee roots. The results showed that the formation of knee roots was significantly affected by the soil water table (P < 0.05). The middle water table was more conducive to the formation of knee roots. In the middle water table, the 1-aminocyclopropane-1-carboxylic acid (ACC) content and ACC synthase activity were significantly lower in the knee roots than in the underground roots. The knee roots at the young-aged stage showed the highest ACC oxidase activity among the development stages of the knee roots. The ethylene release rate was significantly higher in the knee roots than in the underground roots (P < 0.05). Indole-3-acetic acid (IAA) content first increased, then decreased with knee root development. The periderm cells at the apex of the knee roots were dead and had many intercellular spaces, which was beneficial for the growth of T. ascendens. In conclusion, the middle water table induced the ethylene and IAA production, which promoted the formation of knee roots, which improved roots ventilation and flooding tolerance of T. ascendens. The results obtained can provide information about mechanisms of knee roots formation and provide scientific evidence for the afforestation and management under wetland conditions.

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Broussonetia papyrifera is a widely distributed economic tree species, and it is also a pioneer species in adverse environments. In order to investigate the growth and adaptation mechanism of B. papyrifera under cadmium (Cd) contaminated soil, potted experiments were used with six-month treatments to study Cd enrichment and the transportation, morphological and physiological characteristics of B. papyrifera tissues. The results showed that Cd mainly accumulated in the root when the Cd concentration was high (14.71 mg/kg), and the root biomass was significantly reduced by Cd stress although Cd promoted the growth of seedlings. The bioconcentration factors (BCF) increased with the increase in Cd concentration, and reached the maximum value of 0.21 at 14.71 mg/kg. On the contrary, translocation factor (TF) decreased significantly at 8.28-14.71 mg/kg Cd concentration. Cd not only led to the loose arrangement of the xylem vessels of leaves, but also changed the chlorophyll content. However, B. papyrifera could synthesize organic solutes such as soluble protein, soluble sugar and proline to reduce the intracellular osmotic potential. Our study proved that B. papyrifera has good tolerance to Cd stress and is a pioneer tree species for soil and ecological environment restoration.

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BACKGROUND: The relationship between the lateral femoral anatomic structures and femoral tunnel outlet according to changes in knee flexion and transverse drill angle during femoral tunnel creation in anterior cruciate ligament (ACL) reconstruction remains unclear. PURPOSE: To investigate the relationships between the lateral femoral anatomic structures and femoral tunnel outlet according to various knee flexion and transverse drill angles and to determine appropriate angles at which to minimize possible damage to the lateral femoral anatomic structures. STUDY DESIGN: Controlled laboratory study. METHODS: Simulation of ACL reconstruction was conducted using a 3-dimensional reconstructed knee model from the knees of 30 patients. Femoral tunnels were created using combinations of 4 knee flexion and 3 transverse drill angles. Distances between the femoral tunnel outlet and lateral femoral anatomic structures (minimum safe distance, 12 mm), tunnel length, and tunnel wall breakage were assessed. RESULTS: Knee flexion and transverse drill angles independently affected distances between the femoral tunnel outlet and lateral femoral anatomic structures. As knee flexion angle increased, the distance to the lateral collateral ligament, lateral epicondyle, and popliteal tendon decreased, whereas the distance to the lateral head of the gastrocnemius increased (P < .001). As the transverse drill angle decreased, distances to all lateral femoral anatomic structures increased (P < .001). Considering safe distance, 120°, 130°, or 140° of knee flexion and maximum transverse drill angle (MTA) could damage the lateral collateral ligament; 130° or 140° of knee flexion and MTA could damage the lateral epicondyle; and 110° or 120° of knee flexion and MTA could damage the lateral head of the gastrocnemius. Tunnel wall breakage occurred under the conditions of MTA - 10° or MTA - 20° with 110° of knee flexion and MTA - 20° with 120° of knee flexion. CONCLUSION: Approximately 120° of knee flexion with MTA - 10° and 130° or 140° of knee flexion with MTA - 20° or MTA - 10° could be recommended to prevent damage to the lateral femoral anatomic structures, secure adequate tunnel length, and avoid tunnel wall breakage. CLINICAL RELEVANCE: Knee flexion angle and transverse drill angle may affect femoral tunnel creation, but thorough studies are lacking. Our findings may help surgeons obtain a stable femoral tunnel while preventing damage to the lateral femoral anatomic structures.

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PURPOSE: To determine whether there is asymmetry in the lateral rectus (LR) muscle attachment between both eyes in patients with intermittent exotropia (IXT) with a non-dominant eye, but without amblyopia or anisometropia. METHODS: In total, 109 patients who underwent bilateral lateral rectus recession for IXT were included, 81 with and 28 without eye dominance. The limbus-insertion distance and tendon width of the LR muscle were measured intraoperatively using callipers. The insertion-equator distance (presumed arc of contact), area of contact (arc of contact × tendon width), and torque value (radius of globe × arc of contact) of the LR muscle were calculated based on intraoperative measurements and axial length measured using a partial interferometer. Parameters regarding LR muscle attachment were compared between fellow eyes and between groups. RESULTS: Mean measurements in all parameters related to LR muscle attachments other than tendon width were not different between the two eyes or between groups. The mean tendon width of the non-dominant eye was 9.2 ± 0.7 mm, narrower than the 9.4 ± 0.5 mm width in either eye of patients without dominance (p = 0.020). However, there was no difference in all parameters in 21 pairs of patients after matching. The proportion of patients who showed binocular discrepancies in attachment measurements beyond that attributable to potential measuring errors did not differ between the two groups. CONCLUSIONS: Structural parameters related to LR muscle attachments did not differ based on eye dominance, suggesting that the anatomic structure of LR muscle attachments is not responsible for eye dominance in IXT.

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We propose a new approach to optimization of electrical stimulation of the vestibular nerve and improving the transfer function of vestibular implant. A mathematical model of the vestibular organ is developed based on its anatomy, the model premises, 3D-analysis of MRI and CT images, and mathematical description of physical processes underlying propagation of alternating electric current across the tissues of vestibular labyrinth. This approach was tested in vitro on the rat vestibular apparatus and had been examined anatomically prior to the development of its mathematical model and equivalent electrical circuit. The experimental and theoretical values of changes of the gain-phase characteristics of vestibular tissues in relation to location of the reference electrode obtained in this study can be used to optimize the electrical stimulation of vestibular nerve.

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In order to better understand and implement the treating principle of acupuncture as "consolidating tendons when tendons involved in disorders", the author explored the necessity of this principle in view of "the depth of disorder and depth of needle insertion" through explaining the meanings of it in the classic literature. In combination with the clinical experience, three recognitions were proposed. When treating tendon disorder, the correct recognition of disease is very important. The coordination of ashi points and the distal points enhances the therapeutic effects. Besides, the modern anatomic knowledge is assisted to the selection of acupoints. The study results enrich the connotation of this treating principle, explore the approach to the clinical treatment of the disorders of bones, joints and soft tissues and improve the therapeutic effects.

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In order to better understand and implement the treating principle of acupuncture as "consolidating tendons when tendons involved in disorders", the author explored the necessity of this principle in view of "the depth of disorder and depth of needle insertion" through explaining the meanings of it in the classic literature. In combination with the clinical experience, three recognitions were proposed. When treating tendon disorder, the correct recognition of disease is very important. The coordination of points and the distal points enhances the therapeutic effects. Besides, the modern anatomic knowledge is assisted to the selection of acupoints. The study results enrich the connotation of this treating principle, explore the approach to the clinical treatment of the disorders of bones, joints and soft tissues and improve the therapeutic effects.

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Objective@#The purpose of this study was to a new operative approach for sagittal condylar fractures via a preauricular small incision-based technique and to examine the effectiveness of this approach. @*Methods@#Fifteen patients (19 sides) with sagittal condylar fractures were included in the study. The incision length was approximately 4 cm through the tragus, exposing the superficial temporal vessels, which was then pulled forward. Next, the deep temporal superficial fascia was cut, and the surface of the zygomatic arch and the articular capsule of the temporomandibular joint were exposed. Joint capsule incision was performed, with mandibular condylar fracture fixation under direct vision. We followed up with the patients postoperatively for 6 months with clinical and radiographic examinations. @*Results @#All patients had 1 week postoperation before being discharged, during which 2 cases of mild facial paralysis (with lateral temporal level Ⅱ facial paralysis, with lateral temporal branch level Ⅲ facial paralysis and level Ⅱ zygomatic branch of facial nerve paralysis after treatment) were observed, after given nerve nutrition agents, 2 cases returned to normal within 3 months. No patient exhibited a postoperative delayed fistula infection or other serious complications. Intraoperative occlusion relationships recovered well, and postoperative CTs suggested that the fracture ends and condyles were in good condition. The occlusion relationship was normal for 3 months after surgery, with a degree of opening greater than 30 mm, no play in the joints and no oblique openings being observed, and reexamination 6 months after the surgery revealed no obvious scars.@*Conclusion@#This surgical method involves a small incision and clear anatomic structures and avoids damage to the facial nerve. This method provides better surgical vision for treatment of sagittal condylar fractures, is safe and convenient, and deserves clinical recommendation.

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Objective To provide data support for safe and accurate lower cervical pedicle screw implantation by observing the lower cervical pedicle and its relationship between the adjacent spinal cord, endorhachis, nerve root and vertebral artery, particularly its relation-ship between the endorhachis and epidural sinus. Methods Measured the C3 ~ C7 cervical sample of 32 adult with vernier caliper and pro-tractor, including the distance between cervical pedicle and its upper and lower nerve root, the distance between cervical pedicle and en-dorhachis, the distance between cervical pedicle and vertebral artery, and the distance between cervical pedicle and epidural sinus. Results The distance betweencervical pedicle and its upper nerve root was 1. 18~1. 40 mm, the distance between cervical pedicle and its lower nerve root was 2. 33~3. 11 mm;the distance between cervical pedicle and endorhachis was 2. 75~3. 33 mm;and there was nearly no distance be-tween cervical pedicle and vertebral artery and epidural sinus. Conclusion Cervical pedicle has a very close relationship with spinal cord, endorhachis, vertebral artery and nerve root, and the cervical pedicle is narrow and small. Therefore, it requires fully assess accroding to in-dividualization so as to prevent injury of important anatomical structures.

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Objective To verify IMRT plans in point,planar and 3D dose,and to concretely analyze the dose differences of 3D anatomic structure based on Gamma passing rate.Methods Thimble ion-chamber,Matrixx and ArcCheck were separately used to measure six nasopharyngeal carcinoma treatment plans and six lung cancer treatment plans.The dose measurement deviation of the center point was compared as well as the Gamma passing rate of dose verification under the criteria of both 3％/3 mm and 2％/2 mm,the group t-test and one-way ANOVA were also proceeded.3DVH system was used to analyze the dose measurement deviation of target volume (TV) and organ at risk (OAR) through DVH.Results For IMRT and VMAT treatment plans,the mean deviation of point dose was (0.59 ± 1.31) ％ and (-1.00 ± 1.03)％ respectively,and the maximum deviation was less than 3％.Under the criterion of 3％/3 mm,the Gamma passing rate measured by Matrixx,ArcCheck and 3DVH for IMRT plans was 96.28％,97.55％ and 99.02％ respectively,and for VMAT plans,the corresponding results of three different detectors were 97.24％,99.67％ and 98.48％.The results analyzed and compared by 3DVH showed that even under the condition of high Gamma pass rate (more than 95％ for a Gamma criterion of 3％/3 mm),the DVH metrics of both TV and OAR in two cases (account for 16.7％ of the total plan) were significantly different on the clinical parameters,including GTV,spinal cord and brain stem etc.Conclusions The analysis of dose difference of the measurement results based on Gamma pass rate and on anatomic structure of 3D images can more effectively evaluate the influence of dose error to the implementing of clinical plan and the impact to the clinical treatment.

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AIM: The aim of the present study was to determine the prevalence, radiographic appearance, and characteristics of zygomatic air cell defects (ZACD) in digital panoramic radiographs in a North Indian population. METHODS: The dental panoramic radiographs of 800 outpatients were selected from the records of the department, and were examined retrospectively to evaluate the variations and characteristics of ZACD. Groups were compared by χ(2) analysis for the presence of ZACD. RESULTS: ZACD were identified in 46 of 800 patients, giving an overall prevalence of 5.7%. Patients with ZACD were aged 4-60 years, with a mean age of 31.43 years. Most patients with ZACD were in their thirties. ZACD showed a no gender predilection; 29 of 46 patients were male (63.4%), and 17 were female (36.6%). Thirty cases (65.2%) of ZACD were unilateral, with 20 cases occurring on the right side. In 16 cases (34.8%), ZACD was bilateral. Forty four of the defects were unilocular, and two of the defects were multilocular. CONCLUSION: Digital panoramic radiographs are considered better than conventional panoramic radiographs in locating ZACD. Further, the presence of ZACD might be valuable for age estimations, to some extent. Further studies are required to investigate the pneumatization of articular eminence before puberty.

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Objective To explore the feasibility and advantage of fluoroscope in identification of brain structures in nude mice with green fluorescent protein (GFP) expression. Methods We laid the whole brain separated from 8-week adult nude mice with GFP expression into SLY mouse brain blocker to produce slices of 1 or 0.9 mm thickness; and then,25 μm-thickness frozen sections were cut.Fluoroscope was employed to observe the morphological structure to define their anatomic structures with reference to The Mouse Brain in Stereotaxic Coordinates compiled by Paxinos. After the observation,these frozen sections were performed Nissi staining for contrast. Results Different structures can be identified by their distinct fluorescence intensity:the dense areas of nuclei,Nissl bodies and nerve tract showed low fluorescence intensity; while the structures around the areas of nuclei and nerve tract,such as,the plexiform layer of olfactory bulb and the molecular layer of cerebella,showed high fluorescence intensity.The fluorescence intensity was attenuated obviously after Nissl staining; the visualized structural information observed under stereomicroscope was in accordance with that viewed by fluoroscope.Conclusion The identification of brain structure in nude mice with GFP by fluoroscope can serve as an experimental platform being applied in the anatomic structure positioning in fluorescence tracer experiments.

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O conhecimento das estruturas anatômicas da cavidade oral é fundamental para realização do tratamento protético, bem como das alterações ocorridas nos tecidos ósseo, gengival, muscular e nas articulações têmporo-mandibulares (ATM) com a perda dos dentes. Tais modificações afetam a mastigação, fonação, estética e conforto do paciente. Alterações como reabsorção óssea, hipotonicidade muscular e disfunções da ATM podem ser amenizados com um tratamento reabilitador protético adequado. O entendimento destes processos propicia a realização de uma reabilitação consciente, suprindo as necessidades e expectativas do paciente e proporcionando satisfação ao dentista. Assim, na confecção dessa prótese deve-se respeitar os limites anatômicos e fisiológicos da atuação da musculatura envolvida, analisar a área de rebordo residual, definindo corretamente a área chapeavel, diagnosticar a presença de bridas e torus e no assoalho bucal deve-se respeitar o freio lingual, as inserções dos músculos genioglosso e o fórnix gengival. Conhecer e respeitar as estruturas anatômicas é essencial para a obtenção de sucesso no tratamento de indivíduos desdentados totais

The knowledge of the mouth anatomic structure and the alterations ocurred after teeth lost, are very important for good results in complete denture. After the lost of teeth changes in bone, gingival and muscle tissue and temporomandibular articulations affecting directly in mastication, speaking, esthetic and patient confort. Alterations like bone resorption, muscle hipotonicity and temporomandibular disorders can be minimized with an adequate prosthetic rehabilitation treatment. The understanding of these process leads to a conscious prosthetic rehabilitation, suppling the needs and expectations of the patient and providing satisfaction to dentist. So, in the fabrication of complete denture the anatomic limit and the post damming should be respect; the residual bone area should be analysed; the presence of lingual frenun, torus, insertion of genioglosso muscle and lingualgingival fornix should be diagnosed. Knowing and respecting the mouth anatomic structures is essencial to achieve success in prosthesis treatment of edentulous patient

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Objective To assess the clinical value of multi-slice CT(MSCT)in showing the ear lesions.Methods MSCT high resolution scan images of 52 ears of 11 normal adult and 15 patients with hearing loss were reconstructed,by bone plus with 0.625 mm collimation,9.6 cm small field of vision(FOV)and 0.3 mm reconstruction interval before volume rendering(VR)and multiplanar reformation(MPR)were performed.Results VR,MIP and MPR images of 40 normal ears clearly showed the complicated anatomic structures.VR,MIP and MPR images of twelve abnormal ears displayed 3 ears of malformations of crus longum incudis,1 ear of destructed semicircular canals,2 ears of malleus dysplasia,2 ears of fusion of incudostapedial joint,2 ears of bony atresia of external auditory complicated with auditory ossicles dysplasia,and 2 ears of crus stapedis dysplasia.MPR is easier to manipulate than VR that has three dimensional effect butis difficult to handle.Conclusion HRCT images,combined with post-processing technique of VR,MIP and MPR,can clearly show the anatomic structures and lesions of middle and inner ears.

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Objective To provide basis for identification and utilization of Phytolacca decandra. Methods Anatomic structures of different-aged storing roots, stems and leaves of P. decandra were studied by light microscope and histochemical localization. Results Different-aged vegetative organs have active proteins except the young roots, and the protein used to exist in parenchyma cells. ConclusionProtein content in young stems is the highest and the grain size is bigger than the other organs, and the protein content and grain size in young stems have notable differences from that in the old leaves, young leaves, old stems and roots. No protein has been found in two-year-old storing roots and extra cambium.