RESUMEN
Anaerobic co-fermentation of swine manure (SM) and apple waste (AW) restricts by the slow hydrolysis of substrates with complex structures, which subsequently leads to low lactic acid (LA) production. Therefore, a novel strategy based on enzymatic pretreatment for improving LA production from anaerobic co-fermentation of SM and AW was proposed in this study. The results indicated that the maximal LA concentration increased from 35.89 ± 1.84 to 42.70 ± 2.18 g/L with the increase of enzyme loading from 0 to 300 U/g VSsubstrate. Mechanism exploration indicated that enzymatic pretreatment significantly promoted the release and hydrolysis of insoluble organic matter from fermentation substrate, thus providing an abundance of reaction intermediates that were directly available for LA production. Additionally, bacteria analysis revealed that the high concentration of LA was associated with the prevalence of Lactobacillus. This study offered an environmental-friendly strategy for promoting SM and AW hydrolysis and provided a viable approach for recovering valuable products.
Asunto(s)
Fermentación , Ácido Láctico , Malus , Estiércol , Animales , Hidrólisis , Ácido Láctico/biosíntesis , Porcinos , Residuos , AnaerobiosisRESUMEN
Anaerobic co-fermentation is a favorable way to convert agricultural waste, such as swine manure (SM) and apple waste (AW), into lactic acid (LA) through microbial action. However, the limited hydrolysis of organic matter remains a main challenge in the anaerobic co-fermentation process. Therefore, this work aims to deeply understand the impact of cellulase (C) and protease (P) ratios on LA production during the anaerobic co-fermentation of SM with AW. Results showed that the combined use of cellulase and protease significantly improved the hydrolysis during the enzymatic pretreatment, thus enhancing the LA production in anaerobic acidification. The highest LA reached 41.02 ± 2.09 g/L within 12 days at the ratio of C/P = 1:3, which was approximately 1.26-fold of that in the control. After a C/P = 1:3 pretreatment, a significant SCOD release of 45.34 ± 2.87 g/L was achieved, which was 1.13 times the amount in the control. Moreover, improved LA production was also attributed to the release of large amounts of soluble carbohydrates and proteins with enzymatic pretreated SM and AW. The bacterial community analysis revealed that the hydrolytic bacteria Romboutsia and Clostridium_sensu_stricto_1 were enriched after enzyme pretreatment, and Lactobacillus was the dominant bacteria for LA production. This study provides an eco-friendly technology to enhance hydrolysis by enzymatic pretreatment and improve LA production during anaerobic fermentation.
Asunto(s)
Celulasas , Malus , Animales , Porcinos , Fermentación , Estiércol/microbiología , Ácido Láctico , Bacterias , Péptido HidrolasasRESUMEN
The anaerobic co-fermentation of orange peel waste (OPW) and waste activated sludge (WAS) for useful short-chain fatty acids (SCFAs) generation presents an environmentally friendly and efficient method for their disposal. This study amied to investigate the effects of pH regulation on OPW/WAS co-fermentation, and found that the alkaline pH regulation (pH 9) significantly enhanced the promotion of SCFAs (11843 ± 424 mg COD/L), with a high proportion of acetate (51%). Further analysis revealed that alkaline pH regulation facilitated solubilization, hydrolysis, and acidification while simultaneously inhibiting methanogenesis. Furthermore, the functional anaerobes, as well as the expressions of corresponding gene involved in SCFAs biosynthesis, were generally improved under alkaline pH regulation. Alkaline treatment might played a critical role in alleviating the toxicity of OPW, resulting in improving microbial metabolic activity. This work provided an effective strategy to recover biomass waste as high-value products, and insightful understanding of microbial traits during OPW/WAS co-fermentation.
Asunto(s)
Citrus sinensis , Aguas del Alcantarillado , Fermentación , Ácidos Grasos Volátiles , Anaerobiosis , Concentración de Iones de HidrógenoRESUMEN
Anaerobic co-fermentation (AcoF) of dairy cattle manure (DCM) and corn straw silage (CSS) for producing lactic acid (LA) and volatile fatty acids (VFAs) was investigated. Batch experiments were conducted at seven different DCM/CSS ratios and at mesophilic and thermophilic temperatures. Results indicated that the highest concentration of LA was 17.50 ± 0.70 g/L at DCM:CSS ratio of 1:3 and thermophilic temperature, while VFAs was 18.23 ± 2.45 g/L at mono-CSS fermentation and mesophilic temperature. High solubilization of thermophilic conditions contributed to LA accumulation in AcoF process. Presence of the CSS increased the relative abundance of Lactobacillus for LA production at thermophilic. Meanwhile, the abundance of Bifidobacterium was increased when CSS was added at mesophilic, which could conduce to VFAs production. This study provides a new route for enhancing the biotransformation of DCM and CSS into short-chain fatty acids, potentially bringing economic benefits to agricultural waste treatment.
Asunto(s)
Estiércol , Ensilaje , Anaerobiosis , Animales , Reactores Biológicos/microbiología , Bovinos , Ácidos Grasos Volátiles , Fermentación , Ácido Láctico , Estiércol/microbiología , Temperatura , Zea maysRESUMEN
A novel approach for the enhancement of phosphorus (P) recovery from Fe bound P compounds (FePs)-bearing sludge by co-fermentation with protein-rich biomass (PRB) is reported. Four PRBs (silkworm chrysalis meal, fish meal, corn gluten meal, and soya bean meal) were used for co-fermentation. The results revealed that PRBs with strong surface hydrophobicity and loose structure favored the hydrolysis and acidogenesis processes. Sulfide produced by PRB could react with FePs to form FeS and promote P release. Due to the neutralization of volatile fatty acids (VFAs) by a relatively high concentration of ammonia, the pH was maintained near neutral and thus prevented the dissolution of metal ions (e.g., Fe and Ca). This was beneficial to save the cost of subsequent P recovery and form high-purity struvite. Compared with the control, the soluble orthophosphate and VFAs increased by 88.3% and 531.3%, respectively, in the co-fermentation system with silkworm chrysalis meal. Cysteine was the important intermediate. The metagenomics analysis indicated that the gene abundances of phosphate acetyltransferase and acetate kinase, which were key enzymes in the acetate metabolism, increased by 117.7% and 52.2%, respectively. The gene abundances of serine O-acetyltransferase and cysteine synthase increased by 63.4% and 54.4%, respectively. Cysteine was primarily transformed to pyruvate and sulfide. This study provides an environment-friendly strategy to simultaneously recover P and VFAs resources from FePs-bearing sludge and PRB waste.
Asunto(s)
Fósforo , Aguas del Alcantarillado , Animales , Biomasa , Cisteína/metabolismo , Ácidos Grasos Volátiles/metabolismo , Fermentación , Concentración de Iones de Hidrógeno , Proteínas , Aguas del Alcantarillado/química , SulfurosRESUMEN
Resource utilization is an effective way to cope with the rapid increase of kitchen waste and excess sludge, and volatile fatty acids produced by anaerobic fermentation is an important way of recycling organic waste. However, the single substrate limits the efficient production of volatile fatty acids. In recent years, volatile fatty acids produced by anaerobic co-fermentation using different substrates has been widely studied and applied. In this paper, we analyze the characteristics of fermentation to produce acid using kitchen waste and excess sludge alone or mixture. Influences of environmental factors and microbial community structure on the type and yield of volatile fatty acids in the anaerobic fermentation system are discussed in detail. Moreover, we propose future research directions, to provide a reference for recycling kitchen waste and excess sludge.
Asunto(s)
Microbiota , Aguas del Alcantarillado , Anaerobiosis , Reactores Biológicos , Ácidos Grasos Volátiles , Fermentación , Concentración de Iones de Hidrógeno , Compuestos OrgánicosRESUMEN
Resource utilization is an effective way to cope with the rapid increase of kitchen waste and excess sludge, and volatile fatty acids produced by anaerobic fermentation is an important way of recycling organic waste. However, the single substrate limits the efficient production of volatile fatty acids. In recent years, volatile fatty acids produced by anaerobic co-fermentation using different substrates has been widely studied and applied. In this paper, we analyze the characteristics of fermentation to produce acid using kitchen waste and excess sludge alone or mixture. Influences of environmental factors and microbial community structure on the type and yield of volatile fatty acids in the anaerobic fermentation system are discussed in detail. Moreover, we propose future research directions, to provide a reference for recycling kitchen waste and excess sludge.
Asunto(s)
Anaerobiosis , Reactores Biológicos , Ácidos Grasos Volátiles , Fermentación , Concentración de Iones de Hidrógeno , Microbiota , Compuestos Orgánicos , Aguas del AlcantarilladoRESUMEN
The effects of food wastes (FW) composition and zero-valent iron (ZVI) on the volatile fatty acids (VFAs) generation, bacterial community succession and related metabolic functions during long-term FW and waste activated sludge (WAS) co-fermentation were investigated. The VFAs production in the carbohydrate-enriched reactor was approximately 3.0-folds of that in FW reactor. The ZVI contributed to the VFAs promotion by 3.6- and 6.7-folds in carbohydrate-enriched and FW reactors, respectively. Firmicutes (20.1-74.7%), Actinobacteria (0.9-26.3%), Bacteroidetes (3.4-65.7%), and Proteobacteria (9.1-28.5%) were the main bacteria in different fermentation reactors, and they were closely associated with the fermentation substrates and ZVI. Further analysis demonstrated that the key metabolic capacity (i.e. amino acid, carbohydrate and energy metabolism) and the genetic expressions of enzymes (i.e. fabA, fabZ, accA and accB) involved in VFAs generation were also related to FW composition, and were improved by the ZVI, which accounted for the significant VFAs promotion.
Asunto(s)
Microbiota , Eliminación de Residuos , Anaerobiosis , Reactores Biológicos , Ácidos Grasos Volátiles , Fermentación , Alimentos , Concentración de Iones de Hidrógeno , Hierro , Aguas del AlcantarilladoRESUMEN
Thiosulfinate, a nature antibiotic, existed in all parts of Allium thereby accumulating in kitchen waste vastly. However, few literatures were available related to its influence on volatile fatty acids (VFA) and hydrogen production when kitchen waste digestion technology was applied. This study aimed to explore the inhibitory effect and the relevant mechanism. Experimental results showed that the hydrogen accumulation decreased from 23.2 ± 0.8 to 8.2 ± 0.1 mL/g VSS (volatile suspended solid) and maximal total VFA yield decreased from 765.7 ± 21.2 to 376.4 ± 21.7 mg COD (chemical oxygen demand)/g VSS when the dosage of thiosulfinate increased from 0 to 12.5 µg/g VSS. The mechanism study indicated, compared with control group, that the butyric acid decreased from 59% to 20.1% of total VFA yield when reactor in present of 12.5 µg/g VSS thiosulfinate. Moreover, the relative activities of functional enzymes were inhibited 73.4% (butyryl-CoA) and 72.7% (NADH), respectively.
Asunto(s)
Eliminación de Residuos , Aguas del Alcantarillado , Anaerobiosis , Reactores Biológicos , Ácidos Grasos Volátiles , Fermentación , Alimentos , Hidrógeno , Concentración de Iones de HidrógenoRESUMEN
This study investigated the acidogenic and microbiological perspectives in the anaerobic co-fermentation of waste-activated sludge (WAS) mixed with corn stalk (CS) and pig manure (PM). The volatile fatty acids (VFAs) increased dramatically to over 5000 mg COD/L accumulation just within 4-5 days with the feedstock carbon to nitrogen (C/N) ratio regulation of 20/1. The CS and PM addition enhanced the compressibility of fermentation residuals by increasing the particle distribution spread index (DSI). Moreover, the external carbon addition conduced to bacterial consortia diversity rising and uneven population distribution in the co-fermentation, which contributed to VFAs accumulation potentially. The organic loading rate (OLR) correlated with bacterial community closely at the early stage (days 1-5), while the oxidation-reduction potential (ORP) and pH played more important roles on bacterial consortia at the terminal stage (days 6-10). The C/N ratio adjustment by CS and PM and proper optimizations of OLR, pH, and ORP at various running stages facilitated VFA accumulation during the co-fermentation operation.
Asunto(s)
Ácidos Grasos Volátiles , Estiércol , Aguas del Alcantarillado , Zea mays , Anaerobiosis , Animales , Bacterias , Reactores Biológicos , Metabolismo de los Hidratos de Carbono , Carbono , Fermentación , Concentración de Iones de Hidrógeno , Aguas del Alcantarillado/microbiología , PorcinosRESUMEN
The aim of this study is to investigate the bio-H2 production via dry anaerobic co-fermentation of organic fraction of municipal solid waste (OFMSW) with protein and calcium-rich substrates such as gelatin solid waste (GSW) and paperboard mill sludge (PMS). Co-fermentation of OFMSW/GSW/PMS significantly enhanced the H2 production (HP) and H2 yield (HY). The maximum HP of 1082.5±91.4 mL and HY of 144.9±9.8 mL/gVSremoved were achieved at a volumetric ratio of 70% OFMSW:20% GSW:10% PMS. COD, carbohydrate, protein and lipids conversion efficiencies were 60.9±4.4%, 71.4±3.5%, 22.6±2.3% and 20.5±1.8% respectively. Co-fermentation process reduced the particle size distribution which is favorably utilized by hydrogen producing bacteria. The mean particle size diameters for feedstock and the digestate were 939.3 and 115.2µm, respectively with reduction value of 8.15-fold in the mixtures. The volumetric H2 production increased from 4.5±0.3 to 7.2±0.6 L(H2)/L(substrate) at increasing Ca(+2) concentrations from 1.8±0.1 to 6.3±0.5 g/L respectively.