RESUMEN
Bioremediation became a promising technology to resolve arsenic (As) contamination in aquatic environment. Since monoculture such as microalgae or bacteria was sensitive to environmental disturbance and vulnerable to contamination, green microalgae Chlorella vulgaris and arsenite (As(III)) - oxidizing bacteria Pseudomonas sp. SMS11 were co-cultured to construct algal-bacterial consortia in the current study. The effects of algae-bacteria (A:B) ratio and exposure As(III) concentration on algal growth, As speciation and metabolomic profile were investigated. Algal growth arrested when treated with 100 mg/L As(III) without the co-cultured bacteria. By contrast, co-cultured with strain SMS11 significantly enhanced As tolerance in C. vulgaris especially with A:B ratio of 1:10. All the As(III) in culture media of the consortia were oxidized into As(V) on day 7. Methylation of As was observed on day 14. Over 1% and 0.5% of total As were converted into dimethylarsinic acid (DMA) after 21 days cultivation when the initial concentrations of As(III) were 1 and 10 mg/L, respectively. Metabolomic analysis was further performed to reveal the response of consortia metabolites to external As(III). The enriched metabolomic pathways were associated with carbohydrate, amino acid and energy metabolisms. Tricarboxylic acid cycle and glyoxylate and dicarboxylate metabolism were upregulated under As stress due to their biological functions on alleviating oxidative stress and protecting cells. Both carbohydrate and amino acid metabolisms provided precursors and potential substrates for energy production and cell protection under abiotic stress. Alterations of the pathways relevant to carbohydrate or amino acid metabolism were triggered by energy requirement.
Asunto(s)
Arsénico , Chlorella vulgaris , Microalgas , Arsénico/metabolismo , Pseudomonas/metabolismo , Oxidación-Reducción , Aminoácidos/metabolismo , Carbohidratos , BiomasaRESUMEN
Limited information is available on the characteristics of algal-bacterial aerobic granular sludge (AGS) treating real wastewater, especially on its alginate-like exopolymers (ALE) production. In addition, the effect of target microalgae species inoculation on the system performance has not been fully understood. This study aimed to reveal the effect of microalgae inoculation on the characteristics of algal-bacterial AGS and its ALE production potential. Two photo-sequencing batch reactors (PSBR) were employed, namely R1 with activated sludge and R2 with Tetradesmus sp. and activated sludge being inoculated, respectively. Both reactors were fed with locally sourced municipal wastewater and operated for 90 days. Algal-bacterial AGS were successfully cultivated in both reactors. No significant difference was observed between the performances of R1 and R2, reflecting that the inoculation of target microalgae species may not be crucial for the development of algal-bacterial AGS when treating real wastewater. Both reactors achieved an ALE yield of about 70 mg/g of volatile suspended solids (VSS), indicating that a substantial amount of biopolymer can be recovered from wastewater. Interestingly, boron was detected in all the ALE samples, which might contribute to granulation and interspecies quorum sensing. The enrichment of lipids content in ALE from algal-bacterial AGS treating real wastewater reveals its high resource recovery potential. Overall, the algal-bacterial AGS system is a promising biotechnology for simultaneous municipal wastewater treatment and resource (like ALE) recovery.
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Microalgas , Aguas Residuales , Aguas del Alcantarillado/microbiología , Eliminación de Residuos Líquidos , Alginatos , Matriz Extracelular de Sustancias Poliméricas , Reactores Biológicos/microbiología , Aerobiosis , BacteriasRESUMEN
This study reviews the development of the ability of microalgae to remove emerging contaminants (ECs) from wastewater. Contaminant removal by microalgae-based systems (MBSs) includes biosorption, bioaccumulation, biodegradation, photolysis, hydrolysis, and volatilization. Usually, the existence of ECs can inhibit microalgae growth and reduce their removal ability. Therefore, three methods (acclimation, co-metabolism, and algal-bacterial consortia) are proposed in this paper to improve the removal performance of ECs by microalgae. Finally, due to the high removal performance of contaminants from wastewater by algal-bacterial consortia systems, three kinds of algal-bacterial consortia applications (algal-bacterial activatedsludge, algal-bacterial biofilm reactor, and algal-bacterial constructed wetland system) are recommended in this paper. These applications are promising for ECs removal. But most of them are still in their infancy, and limited research has been conducted on operational mechanisms and removal processes. Extra research is needed to clarify the applicability and cost-effectiveness of hybrid processes.
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Oxytetracycline (OTC) and enrofloxacin (EFX) pollution in surface water are very common. Using the algal-bacterial consortia system to remove antibiotics remains to be further studied. In this study, the algal growth and sewage purification performance were studied in an algal-bacterial consortia system with different concentrations of antibiotics. The enzyme activity, malondialdehyde content, chlorophyll-a content, extracellular polysaccharide, and protein content of algae were also tested. It was found that the algal growth was promoted by low-dose antibiotics, 21.83% and 22.11% promotion at 0.1 mg L-1 OTC and EFX, respectively. The nutrients and antibiotics removals of the low-dose groups (OTC <5 mg L-1, EFX <1 mg L-1) were not affected significantly. More than 70% of total organic carbon and total phosphorus, and 97.84-99.76% OTC, 42.68-42.90% EFX were removed in the low-dose groups. However, the algal growth was inhibited, and the nutrients removals performance also declined in the high-concentration groups (10 mg L-1 OTC, 5 mg L-1 EFX). The superoxide dismutase and catalase activity, and malondialdehyde content increased significantly (P < 0.05), indicating the increased activity of reactive oxygen species. In addition, the decreased chlorophyll-a content, thylakoid membrane deformation, starch granules accumulation, and plasmolysis showed that the algal physiological functions were affected. These results showed that the algal-bacterial consortia system was more suitable to treat low-concentration antibiotics and provided basic parameters for the consortia application.
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Oxitetraciclina , Aguas del Alcantarillado , Bacterias , Enrofloxacina , FósforoRESUMEN
In this study, the effects of nutrient and dissolved organic matter removal, stress resistance (DNA methylation), and the algae-bacteria dynamic ratio of algal-bacterial consortia in actual municipal wastewater were investigated. Results indicate that the presence of a Chlorella vulgaris-Bacillus licheniformis consortium had profound effects. The removal rates of total nitrogen, ammonium, orthophosphate phosphorus and chemical oxygen demand were 88.82%, 84.98%, 84.87% and 82.25%, respectively. Protein-like substances, which are difficult to degrade in the natural water environment, were significantly degraded in actual municipal wastewater. Furthermore, the microbial diversity was measured. The algal-bacterial consortium did not disrupt the microbial in-situ diversity of the actual municipal wastewater under suitable conditions. The global nuclear DNA methylation level peaked at 7.80%. These results help to understand the effects of algal-bacterial consortia on nutrient and pollutant removal and adaptability in actual municipal wastewater.
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Bacillus licheniformis/fisiología , Chlorella vulgaris/fisiología , Purificación del Agua/métodosRESUMEN
A comprehensive mathematical model was constructed to evaluate the complex substrate and microbial interaction in algal-bacterial photo sequencing batch reactors (PSBR). The kinetics of metabolite, growth and endogenous respiration of ammonia oxidizing bacteria, nitrite oxidizing bacteria and heterotrophic bacteria were coupled to those of microalgae and then embedded into widely-used activated sludge model series. The impact of light intensity was considered for microalgae growth, while the effect of inorganic carbon was considered for each microorganism. The integrated model framework was assessed using experimental data from algal-bacterial consortia performing sidestream nitritation/denitritation. The validity of the model was further evaluated based on dataset from PSBR performing mainstream nitrification. The developed model could satisfactorily capture the dynamics of microbial populations and substrates under different operational conditions (i.e. feeding, carbon dosing and illuminating mode, light intensity, influent ammonium concentration), which might serve as a powerful tool for optimizing the novel algal-bacterial nitrogen removal processes.
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Reactores Biológicos , Desnitrificación , Aguas del Alcantarillado , Nitrificación , Nitritos , NitrógenoRESUMEN
Aquaponics is a promising technology combining aquaculture with hydroponics. In this study, algal-bacterial consortia were introduced into aquaponics, i.e., algal-bacterial based aquaponics (AA), to improve the nitrogen utilization efficiency (NUE) of aquaponics. The results showed that the NUE of AA was 13.79% higher than that of media-based aquaponics (MA). In addition, higher NO3- removal by microalgae assimilation led to better water quality in AA, which made up for the deficiencies of poor aquaponic management of nitrate. As a result of lower NO3- concentrations and dramatically higher dissolved oxygen (DO) concentrations caused by microalgae photosynthesis in the photobioreactor, the N2O emission of AA was 89.89% lower than that of MA, although nosZ gene abundance in MA's hydroponic bed was approximately 30 times over that in AA. Considering the factors mentioned above, AA would improve the sustainability of aquaponics and have a good application foreground.
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Acuicultura , Nitrógeno , Bacterias , Hidroponía , NitratosRESUMEN
The effects of algal inoculation on chemical oxygen demand (COD) and total nitrogen (TN) removal, and indigenous bacterial dynamics were investigated in municipal wastewater. Experiments were conducted with municipal wastewater inoculated with either Chlorella vulgaris AG10032, Selenastrum gracile UTEX 325, or Scenedesmus quadricauda AG 10308. C. vulgaris and S. gracile as fast growing algae in municipal wastewater, performed high COD and TN removal in contrast to Sc. quadricauda. The indigenous bacterial dynamics revealed by 16S rRNA gene amplification showed different bacterial shifts in response to different algal inoculations. The dominant bacterial genera of either algal case were characterized as heterotrophic nitrifying bacteria. Our results suggest that selection of indigenous bacteria that symbiotically interact with algal species is important for better performance of wastewater treatment.