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This comprehensive review explores furfural production from agricultural residues, focusing on its significance as a low-volume, high-value asset crucial for environmental sustainability. It covers diverse production technologies, recent advancements, and applications in agriculture, evaluating furfural's potential to enhance crop resilience and yield. Showing its role in a circular economy, the review discusses how furfural can replace conventional petrochemical processes, thereby reducing environmental impact. Case studies, such as successful implementations with cotton biomass byproducts, illustrate furfural's practical applications and environmental benefits. The study underscores the need for ongoing research, supportive policies, and furfural's growing role in sustainable agriculture and industry. It is focused on furfural's essential contribution to promoting environmental stewardship and sustainable practices. By examining furfural's role as a value-added product from agricultural residues, this review provides insights into its economic viability and potential challenges.

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This review investigates innovative strategies for upcycling agricultural residues into valuable pharmaceutical compounds. The improper disposal of agricultural residues contributes to significant environmental issues, including increased greenhouse gas emissions and ecosystem degradation. Upcycling offers a sustainable solution, transforming these residues into high-value bioproducts (antioxidants, antitumor agents, antidiabetic compounds, anti-inflammatory agents, and antiviral drugs). Nanotechnology and microbial biotechnology have a crucial role in enhancing bioavailability and targeted delivery of bioactive compounds. Advanced techniques like enzymatic hydrolysis, green solvents, microwave processing, pyrolysis, ultrasonic processing, acid and alkaline hydrolysis, ozonolysis, and organosolv processes are explored for their effectiveness in breaking down agricultural waste and extracting valuable compounds. Despite the promising potential, challenges such as variability in residue composition, scalability, and high costs persist. The review emphasizes the need for future research on cost-effective extraction techniques and robust regulatory frameworks to ensure the safety, efficacy, and quality of bioproducts. The upcycling of agricultural residues represents a viable path towards sustainable waste management and production of pharmaceutical compounds, contributing to environmental conservation and public health improvements. This review provides an analysis of the current literature and identifies knowledge gaps, offering recommendations for future studies to optimize the use of agricultural residues in the drug industry.

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Aquaculture farming generates a significant amount of wastewater, which has prompted the development of creative bioprocesses to improve wastewater treatment and bioresource recovery. One promising method of achieving these aims is to directly recycle pollutants into microbe-rice bran complexes, which is an economical and efficient technique for wastewater treatment that uses synergetic interactions between algae and bacteria. This study explores novel bioaugmentation as a promising strategy for efficiently forming microbial-rice bran complexes in unsterilized aquaculture wastewater enriched with agricultural residues (molasses and rice bran). Results found that rice bran serves a dual role, acting as both an alternative nutrient source and a biomass support for microalgae and bacteria. Co-bioaugmentation, involving the addition of probiotic bacteria (Bacillus syntrophic consortia) and microalgae consortiums (Tetradesmus dimorphus and Chlorella sp.) to an existing microbial community, led to a remarkable 5-fold increase in microbial-rice bran complex yields compared to the non-bioaugmentation approach. This method provided the most compact biofloc structure (0.50 g/L) and a large particle diameter (404 µm). Co-bioaugmentation significantly boosts the synthesis of extracellular polymeric substances, comprising proteins at 6.5 g/L and polysaccharides at 0.28 g/L. Chlorophyta, comprising 80% of the total algal phylum, and Proteobacteria, comprising 51% of the total bacterial phylum, are emerging as dominant species. These microorganisms play a crucial role in waste and wastewater treatment, as well as in the formation of microbial-rice bran complexes that could serve as an alternative aquaculture feed. This approach prompted changes in both microbial community structure and nutrient cycling processes, as well as water quality. These findings provide valuable insights into the transformative effects of bioaugmentation on the development of microbial-rice bran complexes, offering potential applications in bioprocesses for waste and wastewater management.

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This research explores the integration of corn husk nanocellulose (CHNc) and pineapple leaf nanocellulose (PLNc) as reinforcing agents in a carboxymethyl cellulose-based film derived from durian husk (CMCDH). Through a solvent-casting method, composite films were fabricated with varying nanocellulose contents (15, 30, and 45 wt%). Analysis using Fourier transform infrared spectroscopy and x-ray diffraction confirmed the effectiveness of alkaline and bleaching treatments in eliminating noncellulosic components. Transmission electron microscopy image revealed the rod-like morphology of CHNc and PLNc, with dimensions approximately 206.5 × 7.2 nm and 150.7 × 6.5 nm, respectively. The inclusion of nanocellulose decreased the transparency of CMCDH films while enhancing their tensile strength, thermal stability, and water vapor transmission rate. Notably, CMCDH/PLNc(30%) exhibited the highest tensile strength at 5.06 ± 0.83 MPa, representing a remarkable 220% increase compared to CMCDH biofilm. Thermogravimetric analysis and differential scanning calorimeter results indicated that nanocellulose incorporation delayed the film's decomposition temperature by approximately 10°C. Moreover, CMCDH/PLNc(30%) demonstrated the lowest water vapor transmission rate, marking a 20% improvement. However, the film's properties were compromised at the highest nanocellulose content (45 wt%) due to observed fiber aggregation, as revealed by scanning electron microscopy analysis.

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This study examines the pyrolysis of agricultural residues, namely, coconut shells, rice husks, and cattle manure, in a vertical fixed-bed reactor at varying temperatures from 300 to 800 degrees Celsius for biochar production. The research aimed to evaluate the potential of biochar as biofuels, adsorbents, and soil amendments. Proximate, ultimate, and elemental analyses were conducted to determine their composition and caloric values. Several analytical techniques were used in the physical and chemical characterization of the biochar (SEM, FTIR, BET). The results indicated that the highest SBET values were achieved under different conditions for each biochar: 89.58 m2/g for BC-CS-700, 202.39 m2/g for BC-RH-600, and 42.45 m2/g for BC-CD-800. Additionally, all three biochars exhibited the highest caloric values at 600 °C. The results showed that 600 °C is the general optimal temperature to produce biochar from an assortment of biomass materials, considering their use for a variety of purposes. BC-CS-800 had the highest elemental carbon content at 93%, accompanied by a relative decrease in oxygen content. The van Krevelen diagram of biochar products shows that biochars derived from coconut shells and rice husks are suitable for use as fuels. Furthermore, FTIR analysis revealed the presence of oxygen-containing functional groups on the biochar surface, enhancing their pollutant adsorption capabilities. This study provides valuable insights into the scalable and environmentally sustainable production of biochar, emphasizing its role in improving soil quality, increasing energy density, and supporting sustainable agricultural practices.

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Although the formation, turnover, and accumulation of soil organic carbon (SOC) are driven by different fertilizer inputs and their subsequent microbial-mediated transformation, the relationship between changes in plant-derived and microbial-derived components and soil microbial life history strategies under different fertilization regimes has not been well explored. In this study, the changes in microbial necromass carbon (MNC), lignin phenols, and glomalin-related soil protein (GRSP), as well as soil microbial life history strategy were determined in a 16-year field experiment in response to different fertilization regimes, including a no-fertilizer control (C), conventional chemical NPK fertilization (NPK), and partial substitutions of the NPK in chemical fertilizers with a low (30 %) or high (60 %) level of straw (0.3S and 0.6S) or cattle manure (0.3M and 0.6M). The results showed that total lignin phenol content and its contribution to SOC were significantly increased by 88.7 % and 74.2 %, respectively, in high-level straw substitution treatment as compared to chemical fertilization. Both high-level straw and cattle manure substitution increased MNC and total GRSP contents, but did not alter their contributions to SOC compared to chemical fertilization. In fertilized treatments, the high-level cattle manure substitution had the lowest and highest bacterial and fungal K/r ratio, respectively. Bacterial K/r ratio was an important factor in predicting bacterial necromass carbon content and there was a significant negative correlation between them. The ratio of ectomycorrhizal to saprotrophic fungi and fungal diversity were important factors for predicting lignin phenol and GRSP contents, respectively. In addition, the SEMs modeling indicated that straw substitution directly affected lignin phenol and MNC accumulation, whereas cattle manure substitution indirectly affected MNC accumulation by affecting microbial life history strategies. In conclusions, agricultural residues inputs support the formation of a multiple carbon pool of SOC compared to chemical fertilization; and microbial life history strategy is an important driver of SOC formation and affects SOC accumulation and stability in agroecosystems.

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All the countries in the world are now searching for renewable, environmentally friendly alternative fuels due to the shortage and environmental problems related with the usage of conventional fuels. The cultivation of cereal and noncereal crops through agricultural activities produces waste biomasses, which are being evaluated as renewable and viable fossil fuel substitutes. The thermochemical properties and thermal degradation behavior of Sesbania grandiflora residues were investigated for this work. A fluidized bed reactor was used for fast pyrolysis in order to produce pyrolysis oil, char and gas. Investigations were done to analyze the effect of operating parameters such as temperature (350-550 °C), particle size (0.5-2.0 mm), sweeping gas flow rate (1.5-2.25 m3/h). The maximum of pyrolysis oil (44.7 wt%), was obtained at 425 °C for 1.5 mm particle size at the sweep gas flow rate of 2.0 m3/h. Fourier transform infrared spectroscopy and gas chromatography-mass spectrometry methods were used to examine the composition of the pyrolysis oil. The pyrolysis oil is rich with aliphatic, aromatic, phenolic, and some acidic chemicals. The physical characteristics of pyrolysis oil showed higher heating value of 19.76 MJ/kg. The char and gaseous components were also analyzed to find its suitability as a fuel.

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Anaerobic digestion of agricultural waste can contribute to the European renewable energy needs. The 71% of the 20,000 anaerobic digestion plants in operation already uses these agro-waste as feedstock; part of these plants can be converted into two stage processes to produce hydrogen and methane in the same plant. Biomethane enriched in hydrogen can replace natural gas in grids while contributing to the sector decarbonisation. Straw is the most abundant agricultural residue (156 Mt/y) and its conventional final fate is uncontrolled soil disposal, landfilling, incineration or, in the best cases, composting. The present research work focuses on the fermentation of spent mushroom bed, an agricultural lignocellulosic byproduct, composed mainly from wheat straw. The substrate has been characterized and semi-continuous tests were performed evaluating the effect of the hydraulic retention time on hydrogen and volatile fatty acids production. It was found that all the tests confirmed the feasibility of the process even on this lignocellulosic substrate, and also, it was identified HRT 4.0 d as the best option to optimize the productivity of volatile fatty acids (17.09 gCODVFAs/(KgVS*d)), and HRT 6.0 d for hydrogen (7.98 LH2/(KgVS*d)). The fermentation effluent was used in biomethanation potential tests to evaluate how this process affects a subsequent digestion phase, reporting an increase in the energetical feedstock exploitation up to 30%.

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Several energy-related strategies and scenarios have been suggested to address concerns about rising global temperatures. In addition to using renewable energy, the improvement in energy efficiency of conventional systems is also in focus. Policies are already in place in many countries, including India, to address the energy needs of rural and small-scale enterprises by gasifying locally available, diverse agricultural leftovers. Although rice husk and groundnut shell are two commonly used agricultural leftovers in the southern part of India, their appropriate blending must be studied to improve their conversion efficiency in co-gasification. Therefore, the primary objective of this research is to construct a statistical model utilizing response surface methodology (RSM) to analyze the thermochemical co-gasification of the aforementioned biomass materials. Since RSM can predict optimum performance with limited experimental data, this could contribute to the identification of the performance and operating parameters of an open-core gasifier. The model predicts that the mixture containing 20% rice husk and working at an ER of 0.25 and a reduction zone inlet temperature of 879.9 °C will be CO-23.53%, H2-13.97%, and CH4-3.56%. In addition, the lower heating value and gas yield can be as high as 6.17 MJ/Nm3 and 2.369 m3/kg, respectively. This outcome can contribute to the effective utilization of biomass for energy supply in rural areas. However, the economic parameters must be analyzed to implement the same in any region.

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Open biomass burning (OBB) is one of the largest primary emission sources for atmospheric carbonyl compounds, key precursors for ozone and secondary organic aerosol pollution. To clarify the carbonyl emissions, the comprehensive characteristics of C1-C10 carbonyl compounds from open burning of seven typical subtropical biomass in China were investigated in this study, which included subtropical plants and agricultural residues. Total 27 carbonyl compounds were detected. The total EFs were 2824 mg kg-1 with 95% confidence interval (CI) [2418, 3322] for burning subtropical plants and 4080 mg kg-1 with 95% CI [3446, 4724] for burning agriculture residues, respectively. The EFs were 2-3 orders of magnitude larger than previous values in China. Aliphatic aldehydes were the largest group of carbonyl groups, with acetaldehyde, as the most abundant carbonyl species (about 30% contribution). Formaldehyde, acetone, acrolein, glyoxal, methylglyoxal, butanone, isovaleraldehyde, and m-tolualdehyde were also found to be abundant and varying with the types of biomass burnt. Formaldehyde emission ratios to acetonitrile and CO were lower than those in previous studies both for burning plants and agricultural residues. There were significant variabilities in the emission ratios and factors among different types of OBBs. Strong positive correlations were found between carbonyl emissions and CO emissions and water content in biomass; furthermore, total carbonyl concentrations measured in the flaming stage were higher than those in the smoldering one. This study provides important fundamental measurement data on carbonyl emissions from burning typical subtropical plants and agricultural residues, which will help improve the quality of emission inventories and better understand the potential impacts of OBB on regional air quality in southern China.

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Hypertension is a widespread health risk, affecting over a billion people and causing 9 million deaths per year. The Renin-Angiotensin-Aldosterone System (RAAS) is a primary target for hypertension treatment, and it is primarily treated through drugs that inhibit the Angiotensin I-Converting Enzyme (ACE). In addition to pharmacological treatment, various plants are recommended in traditional medicine for blood pressure regulation. This study aimed to produce high-phenolic-content extracts with and without enzymatic assistance from red grape pomace and evaluate their antioxidant capacity and ACE inhibitory potential. The total phenolic content (TPC) was measured, and phenolic identification was performed using HPLC analysis. In addition, the antioxidant capacity and anti-hypertensive potential were determined via in vitro assays. There was no statistical difference in the TPC antioxidant capacity between the extraction methods. Otherwise, when considering the extraction yield, the enzymatic process recovered around 70% more phenolic compounds from the pomace, and the phenolic profile was changed. Enzymatic assistance also significantly increased the ACE inhibitory potential in the grape pomace extract. This study demonstrates the viability of upcycling grape pomace to obtain bioactive compounds and to reduce their environmental impact, and highlights the influence of the enzymatic extraction on the hypotensive potential of the extract.

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Threats of soil quality deterioration and metal pollution have inflicted several parts of the world, apart from the need for surplus crop production. The investigation used biochar prepared from waste biomasses such as wheat and rice straw, kitchen waste, leaf litter, Lantana camara, orange peel, and walnut shell to improve soil quality, reduce As pollution, and enhance plant growth. Biochars were amended at doses of 0%, 2.5%, 5%, and 7.5% and conditioned for 3 months. At a 7.5% dose, the maximum improvements in cation exchange capacity (a 62% increase), anion exchange capacity (a two-fold increase), bulk density (a 31% decrease), porosity (a 32% increase), water holding capacity (an 86% increase), soil respiration (a 32% increase), total carbon (a two-and-a-half-fold increase), total nitrogen (an eleven-fold increase), total phosphorus (3 times rise), total potassium (a two-and-a-half-fold increase), mobile As (a 38% decrease), leachable As (a 53% decrease), and bio-available As (a 56% decrease) were observed. Further, pot experiments revealed augmented biomass growth (61% and 177%), increased length (71% and 209%), and decreased As accumulation (56% and 55%) in the above-ground parts of Bengal gram and coriander plants, respectively. Therefore, the application of biochar was found to enhance the physico-chemical properties of soil, reduce As contamination levels, and improve crop growth. The study recommends using waste biomasses to prepare eco-friendly biochars, which could contribute to advancing sustainable agriculture and the circular economy.

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This study compared two biotrickling filter packing materials for hydrogen sulfide removal. Inlet H2S concentrations and empty-bed retention time were tested on the two biotrickling filters. First reactor (BT1) had immobilized sulfur-oxidizing bacteria on commercial moving-bed media, whereas second reactor (BT2) had sulfur-oxidizing bacteria on sugarcane bagasse. The study found that BT1 performed best at 120 s empty-bed retention time, 422.39 g/m3·h hydrogen sulfide loading rate, resulted in 416 g/m3·h hydrogen sulfide elimination capacity. In contrast, BT2 performed best at 180 s empty-bed retention time, 278.77 g/m3·h hydrogen sulfide loading rate, and 273 g/m3·h elimination capacity was achieved. High-throughput sequencing showed Acidithobacillus spp. dominated the sulfur-oxidizing bacteria consortium. Sugarcane bagasse may receive less hydrogen sulfide loading than moving bed medium under optimal conditions, but its low cost and reasonable removal capacity of hydrogen sulfide -containing industrial gases in a biotrickling filter system make it an excellent alternative packing material.

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Effective substrate utilization with low residual methane yield in the digestate is crucial for the economy and sustainability of biogas plants. The composition and residual methane potential of 29 digestate samples from plants operating at hydraulic retention times of 13-130 days were determined to evaluate the economic viability of extended digestion. Considerable contents of fermentable fractions, such as cellulose (8-23%), hemicellulose (1-18%), and protein (13-22%), were present in the digestate dry matter. The ultimate residual methane yields varied between 55 and 236 ml/g of volatile solids and correlated negatively with the logarithm of the hydraulic retention time (r = -0.64, p < 0.05). Economic analysis showed that extending the retention time in 20 days would be viable for 18 systems if methane were sold for 1.00 €/m3, with gains up to 40 €/year/m3 of newly installed reactor capacity. The results show the importance of operating at sufficient hydraulic retention time.

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This data evaluated the capacity of Bacillus spp. isolated from Thai milk kefir to produce exopolysaccharide (EPS) on cassava pulp and tested its antioxidant and antibacterial properties. Thailand's starch industry generates million tons of cassava pulp, which is underutilized or bio-transformed into higher-value bioproducts. Antioxidant and antibacterial bacterial exopolysaccharides are beneficial in the food, feed, pharmaceutical, and cosmetic industries. Moisture, ash, fat, protein, fiber, starch, sugar, neutral detergent fiber (NDF), acid detergent fiber (ADF), and acid detergent lignin (ADL) were analyzed from cassava pulp as an EPS substrate. After 3 days of bacterial fermentation, EPS generation, culture pH, reducing sugar amount, and bacterial count were recorded. Antioxidant activities and bioactive content including hydroxyl radical scavenging activity, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, ferric reducing antioxidant power (FRAP), total phenolic and flavonoid content (TPC and TFC), and antimicrobial activity against two Nile tilapia pathogens (Streptococcus agalactiae and Staphylococcus aureus) from different Bacillus species were evaluated. Proximate analysis, dinitrosalicylic acid assay, pH value record, bacterial count using spread plate method, antioxidant activity and bioactive content assays via spectrophotometry, and agar disk diffusion were the main approaches. This study used microbial cell factories to convert agro-biowaste, such as cassava pulp, into EPS bioproducts which accords with a bio-circular green economy model.

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Extraction of cellulose nanocrystals (CNCs) from agro-residues has received much attention, not only for their unique properties supporting a wide range of potential applications, but also their limited risk to global climate change. This research was conducted to assess Nile roses (Eichhornia crassipes) fibers as a natural biomass to extract CNCs through an acid hydrolysis approach. Nile roses fibers (NRFs) were initially subjected to alkaline (pulping) and bleaching pretreatments. Microcrystalline cellulose (MCC) was used as control in comparison to Nile rose based samples. All samples underwent acid hydrolysis process at a mild temperature (45 °C). The impact of extraction durations ranging from 5 to 30 min on the morphology structure and crystallinity index of the prepared CNCs was investigated. The prepared CNCs were subjected to various characterization techniques, namely: X-ray diffraction (XRD), FT-IR analysis, Transmission electron microscopy (TEM), and X-ray Photoelectron spectroscopy (XPS). The outcomes obtained by XRD showed that the crystallinity index increased as the duration of acid hydrolysis was prolonged up to 10 min, and then decreased, indicating optimal conditions for the dissolution of amorphous zones of cellulose before eroding the crystallized domains. These data were confirmed by FT-IR spectroscopy. However, a minor effect of hydrolysis duration on the degree of crystallinity was noticed for MCC based samples. TEM images illustrated that a spherical morphology of CNCs was formed as a result of 30 min acid hydrolysis, highlighting the optimal 20 min acid hydrolysis to obtain a fibrillar structure. The XPS study demonstrated that the main constituents of extracted CNCs were carbon and oxygen.

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BACKGROUND: Prolyl endopeptidase from Aspergillus niger (AN-PEP) is a prominent serine proteinase with various potential applications in the food and pharmaceutical industries. However, the availability of efficient and low-cost AN-PEP remains a challenge owing to its low yield and high fermentation cost. RESULTS: Here, AN-PEP was recombinantly expressed in Trichoderma reesei (rAN-PEP) under the control of the cbh1 promoter and its secretion signal. After 4 days of shaking flask cultivation with the model cellulose Avicel PH101 as the sole carbon source, the extracellular prolyl endopeptidase activity reached up to 16.148 U/mL, which is the highest titer reported to date and the secretion of the enzyme is faster in T. reesei than in other eukaryotic expression systems including A. niger and Komagataella phaffii. Most importantly, when cultivated on the low-cost agricultural residue corn cob, the recombinant strain was found to secret a remarkable amount of rAN-PEP (37.125 U/mL) that is twice the activity under the pure cellulose condition. Furthermore, treatment with rAN-PEP during beer brewing lowered the content of gluten below the ELISA kit detection limit (< 10 mg/kg) and thereby, reduced turbidity, which would be beneficial for improving the non-biological stability of beer. CONCLUSION: Our research provides a promising approach for industrial production of AN-PEP and other enzymes (proteins) from renewable lignocellulosic biomass, which provides a new idea with relevant researchers for the utilization of agricultural residues.

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The use of petroleum-based plastic has led to its accumulation in the environment, with negative impacts on the ecosystem and the biota. Polyhydroxyalkanoates (PHAs), biobased and biodegradable plastics produced by microbes, have many commercial applications, however their high production cost means they cannot yet compete with traditional plastics. At the same time, the problem of the growing human population implies that improved crop production is needed to avoid malnutrition. Biostimulants enhance plant growth and have the potential to improve agricultural yields; they can be obtained from biological feedstock, including microbes. Therefore, there is scope for coupling the production of PHAs with that of biostimulants, making the process more cost-efficient and minimizing by-products generation. In this work, low-value agro-zoological residues were processed to obtain PHA-storing bacteria via acidogenic fermentation; PHAs destined for the bioplastic market were extracted, and the protein-rich by-products were turned into protein hydrolysates using different treatment methods, assessing their biostimulant effects in growth trials with tomato and cucumber plants. The results indicate that the best hydrolysis treatment, realizing the highest amount of organic nitrogen (6.8 gN-org/L) while achieving the best PHA recovery (63.2 % gPHA/gTS), is obtained with strong acids. All the protein hydrolysates were effective in improving either roots or leaf development, with various results, depending on the species and the growth method. The acid hydrolysate was the most effective treatment to enhance the development of shoots (21 % increase compared to the control) and roots (16 % increase for the dry weight and 17 % for main root length) of hydroponically-grown cucumber plants, while pot-grown tomatoes, biostimulated via foliar spray, developed bigger shoots (up to 41 %) with the hydrolysate obtained from the alkaline treatment. These preliminary results indicate that simultaneous production of PHAs and biostimulants is feasible, and that commercialization could be achievable given the expected reduction in production costs.

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Bioenergy is becoming very popular, drawing attention as a renewable energy source that may assist in managing growing energy costs, besides possibly affording revenue to underprivileged farmers and rural populations worldwide. Bioethanol made from agricultural residual-biomass provides irreplaceable environmental, socioeconomic, and strategic benefits and can be considered as a safe and cleaner liquid fuel alternative to traditional fossil fuels. There is a significant advancement made at the bench scale towards fuel ethanol production from agricultural lignocellulosic materials (ALCM). These process technologies include pretreatment of ALCM biomass employment of cellulolytic enzymes for depolymerizing carbohydrate polymers into fermentable sugars to effectively achieve it by applying healthy fermentative microbes for bioethanol generation. Amongst all the available process methods, weak acid hydrolysis followed by enzymatic hydrolysis process technique. Recovering higher proficient celluloses is more attractive in terms of economic benefits and long-term environmental effects. Besides, the state of ALCM biomass based bioethanol production methods is discussed in detail, which could make it easier for the scientific and industrial communities to utilize agricultural leftovers properly.

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