RESUMEN

For the first time, a rapid method was proposed to determine the susceptibility of Escherichia coli cells to antibiotics by the example of ampicillin by using a biological sensor based on a slot mode in an acoustic delay line. It has been established that an indicator of the antibiotic activity to microbial cells is the difference between the recorded sensor's signal before and after exposure cells with antibiotic. The depth and frequency of the peaks of resonant absorption in the frequency dependence of the insertion loss of sensor varied after adding an antibiotic with different concentrations to the microbial cells. By using the acoustic sensor based on slot-mode a criterion of E. coli sensitivity to ampicillin was established. The advantages of this method are the ability to carry out the analysis directly in the liquid, the short analysis time (within 10-15 min), and the possibility to reusable sensor.

Asunto(s)

RESUMEN

In this paper, we report a new photoacoustic sensing probe design consisting of two optical fibers. One optical fiber is used for delivering the excitation light pulses. The other one serves as an acoustic delay line to relay the generated PA signal from the target to an outside ultrasound transducer. With the addition of suitable time delay, the original PA signal can be easily separated from the interference signals. To demonstrate this new design, a prototype probe was designed, fabricated and tested. The PA sensing performance was characterized with different concentration of black and red dye solutions. The testing results show that the PA sensing probe can provide good sensitivity and maintain high linearity over a wide range of concentrations. The detection of bovine blood embedded into chicken breast tissue was also conducted to demonstrate its potential usefulness for in-vivo applications.

RESUMEN

The results of the study of the influence of the conductivity of a thin film located with a certain gap above the acoustic delay line on the characteristics of propagating acoustic wave with a quasi-shear-horizontal polarization (SH0) are presented. The delay line was made of Y-X lithium niobate plate with a thickness of 200 µm containing two interdigital transducers for exciting and receiving SH0 wave. The operating frequency range of the delay line was equal to 2.6-3.8 MHz. The 1 mm thick glass plate with a film of the given conductivity applied on one plate's side was placed above this delay line with the some air gap. The change in the phase of output signal of the delay line was measured at the various values of the width of this gap. As films with the various values of the conductivity, tin dioxide films deposited on a glass plate by high-frequency reactive magnetron sputtering in the oxygen-argon mixture were used. It has been shown that the velocity of SH0 wave propagating in the delay line under investigation depends on the film conductivity and the width of the gap between the delay line and conductive film. The dependences of the change in the SH0 wave velocity on a width of the gap between the surface of the delay line and conductive film were constructed, as well as on the film conductivity. The comparison of the experimental results with the theoretical data showed their good agreement.

RESUMEN

Photoacoustic imaging (PAI) is a new biomedical imaging technology currently in the spotlight providing a hybrid contrast mechanism and excellent spatial resolution in the biological tissues. It has been extensively studied for preclinical and clinical applications taking advantage of its ability to provide anatomical and functional information of live bodies noninvasively. Recently, microelectromechanical systems (MEMS) technologies, particularly actuators and sensors, have contributed to improving the PAI system performance, further expanding the research fields. This review introduces cutting-edge MEMS technologies for PAI and summarizes the recent advances of scanning mirrors and detectors in MEMS.