RESUMEN
Noninvasive axillary lymph node staging was investigated using [131I]murine monoclonal antibody B72.3 in 16 patients with breast cancer scheduled for axillary dissection. [131I]B72.3 was injected into ipsilateral finger webs or around the breast biopsy. Scintigraphy to 72 h and gamma-counting/immunohistochemistry of nodes were performed. Specific antibody uptake (%ID/g) and the ratio of specific:nonspecific antibody uptake were not significantly different in tumor-positive versus tumor-negative nodes, suggesting that [131I]B72.3 is unsuitable to discriminate axillary node tumor involvement.
Asunto(s)
Neoplasias de la Mama/diagnóstico por imagen , Radioisótopos de Yodo , Metástasis Linfática/diagnóstico por imagen , Radioinmunodetección , Radiofármacos , Anticuerpos Monoclonales , Anticuerpos Antineoplásicos , Antígenos de Neoplasias/análisis , Neoplasias de la Mama/patología , Femenino , Glicoproteínas/análisis , Humanos , Inmunohistoquímica , Radioisótopos de Yodo/farmacocinética , Ganglios Linfáticos/diagnóstico por imagen , Metástasis Linfática/patología , Estadificación de Neoplasias , Radiofármacos/farmacocinéticaRESUMEN
RDEC-1 (serotype O15) is an attaching and effacing strain of rabbit enteropathogenic Escherichia coli (REPEC) that causes diarrhea in postweanling rabbits. It expresses AF/R1 pili that mediate Peyer's patch M-cell adherence. We investigated Peyer's patch adherence, the presence of virulence genes, ileal brush border aggregation, and pilus expression in 9 strains representing several serotypes of REPEC as well as in two commensal strains. Postweanling rabbits were inoculated with 10(6) organisms and sacrificed at 24 h, and tissues were prepared for examination by light microscopy. Strains B10 and RDEC-1 were also studied at 12 and 72 h postinoculation. All REPEC strains were eaeA positive, expressed pili, and adhered to ileal brush borders. Both commensal strains expressed pili, and one strain adhered to brush borders. All REPEC strains demonstrated some degree of Peyer's patch lymphoid follicle adherence, ranging from diffuse coverage to small patches covering two to three dome epithelial cells. Strains C102 and C110 had genes homologous with the structural subunit gene of the AF/R1 pilus (afrA) of RDEC-1, which correlated with greater degrees of lymphoid follicle adherence and lesser degrees of ileal villus adherence. The observation that all REPEC strains adhere to Peyer's patch epithelium suggests the possibility that human strains of enteropathogenic E. coli (EPEC) might do likewise. EPEC strains might thus serve as mucosal vaccine vectors in humans. Better understanding of the molecular mechanism of REPEC adherence should provide a model for the targeting of the Peyer's patch in humans.
Asunto(s)
Escherichia coli/patogenicidad , Ganglios Linfáticos Agregados/microbiología , Animales , Adhesión Bacteriana , Epitelio/microbiología , Escherichia coli/inmunología , Infecciones por Escherichia coli/inmunología , Infecciones por Escherichia coli/microbiología , Fimbrias Bacterianas/ultraestructura , Íleon/microbiología , Enfermedades Intestinales/microbiología , Microvellosidades/microbiología , ConejosRESUMEN
Mycoleptodiscus indicus, a dematiaceous hyphomycete, was identified as the causal agent of subcutaneous infection in the knee of a 72-year-old male gardener residing in coastal South Carolina. The patient had Wegener's granulomatosis and immunodeficiency. Synovial fluid and biopsy tissue sections from the prepatellar bursa stained with hematoxylin and eosin, periodic acid-Schiff, and Gomori methenamine silver stains revealed branched, septate hyphae and many moniliform hyphal elements. When tissue sections were stained by the Fontana-Masson procedure, melanin pigment in the hyphal cell walls and at the septa was evident. A velvety, dematiaceous mold was isolated from both synovial fluid and the biopsy tissue. Sporulation was induced by exposure of slide cultures on potato dextrose agar to UV light for 12 h at 25 degrees C followed by incubation of the slide cultures at 25 degrees C in the dark for 4 weeks. Clypeate sporodochia consisting of ampulliform, compressed, phialidic conidiogenous cells produced curved, hyaline, one-celled conidia with setulae at one or both ends. Initial treatment with fluconazole for 7 days was not effective, and cultures were positive after treatment. Treatment with amphotericin B with concomitant irrigation and debridement of the affected area followed by treatment with itraconazole resulted in resolution of the infection.
Asunto(s)
Artropatías/microbiología , Rodilla/microbiología , Hongos Mitospóricos/aislamiento & purificación , Micosis/microbiología , Anciano , Bolsa Sinovial , Granulomatosis con Poliangitis/complicaciones , Humanos , Huésped Inmunocomprometido , Rodilla/patología , Masculino , Hongos Mitospóricos/citología , Hongos Mitospóricos/crecimiento & desarrollo , Micosis/complicaciones , Rótula , Coloración y EtiquetadoRESUMEN
Subcutaneous infusion ports (SIP) were inserted for chronic venous access during 329 procedures in 300 patients over the past five years at the University of Michigan Medical Center, with a total follow-up experience of 318 patient years. Seventy-four per cent of the SIP were surgically implanted while patients were hospitalized. The SIP were used for chemotherapeutic agents (83.0 per cent), blood products (29.0 per cent) or hyperosmolar total parenteral nutrition (8.5 per cent) and accessed a median of three occasions. Eighty-four per cent were used in an outpatient setting at least part of the time. Thirty-nine per cent of SIP were associated with complications, including local infection or sepsis (16.4 per cent), thrombosis of the catheter or central vein (9.7 per cent) and extravasation from the port secondary to needle dislodgement (6.4 per cent). The risk of complication was slightly higher in those SIP first used ten to 14 days after placement as compared with those used earlier or later (p less than 0.05). In 23 of 32 episodes, clinically diagnosed local infection unassociated with systemic sepsis or skin necrosis was successfully treated without removal of the port using aggressive intravenous and oral antibiotics. Treatment of thrombosis of the catheter with either urokinase or streptokinase infusion was successful in ten of 15 attempts. Seventy-two (22 per cent) of SIP were eventually removed, either after completion of the chemotherapy (20) or because of a complication (52) with 29 SIP being replaced. There was no correlation between the risk of infection or thrombosis and the perioperative use of antibiotics, frequency of SIP use or preoperative white blood cell count, platelet count, coagulation profile, blood urea nitrogen or albumen concentration. SIP provide an excellent method of chronic venous access, having a lower rate of infection and thrombosis in historical comparison with external vascular access devices.
Asunto(s)
Cateterismo Venoso Central , Centros Médicos Académicos , Transfusión Sanguínea , Cateterismo Venoso Central/efectos adversos , Catéteres de Permanencia/efectos adversos , Quimioterapia , Femenino , Humanos , Infecciones/etiología , Bombas de Infusión , Masculino , Michigan , Persona de Mediana Edad , Nutrición Parenteral Total , Vena Subclavia , Trombosis/etiologíaRESUMEN
Dual photon absorptiometry is a reliable method of assessing bone mineral density. However, distortions of bony architecture, which may not be evident from the low-resolution images provided, can complicate the evaluation and lead to misleading findings. A case is presented which describes the importance of clinical and radiographic correlation.
Asunto(s)
Fracturas Óseas/diagnóstico por imagen , Cintigrafía , Traumatismos Vertebrales/diagnóstico por imagen , Anciano , Clavos Ortopédicos , Huesos/análisis , Huesos/diagnóstico por imagen , Diagnóstico Diferencial , Femenino , Humanos , Aumento de la Imagen , Región Lumbosacra , Minerales/análisis , RadiografíaRESUMEN
Few noninvasive methods are available to diagnose complications following liver transplantation. Hepatobiliary scintigraphy can differentiate rejection from primary biliary complications such as obstruction or extravasation in patients with nonspecific clinical findings such as fever and rising liver function studies. In the following case report, an unexpected biliary leak from a recipient accessory hepatic duct was demonstrated by [99mTc] DISIDA scintigraphy following liver transplantation.
Asunto(s)
Conductos Biliares Intrahepáticos/anomalías , Extravasación de Materiales Terapéuticos y Diagnósticos/diagnóstico por imagen , Trasplante de Hígado , Complicaciones Posoperatorias , Adulto , Extravasación de Materiales Terapéuticos y Diagnósticos/etiología , Femenino , Humanos , Iminoácidos , Compuestos Organometálicos , Cintigrafía , Disofenina de Tecnecio Tc 99mRESUMEN
Metaiodobenzylguanidine (MIBG) locates most pheochromocytomas and neuroblastomas. The tracer is concentrated in intracellular storage vesicles by an active process. Many other neuroendocrine tumors of the amine precursor uptake and decarboxylation (APUD) series have hormonal storage vesicles and, thus, the potential to take up [131I]MIBG. A variety of neuroendocrine tumors in 57 patients were studied 1, 2, and 3 days after 0.5 mCi [131I]MIBG. Views from skull to pelvis were obtained. Results of MIBG scans were compared with all available imaging modalities (including plain radiography, liver scan, ultrasound, computed tomography, and angiography) and surgical exploration. The neuroendocrine nature of the tumor was determined by histology, immunohistochemistry, electron microscopy, and the assay of appropriate biogenic amines and peptide hormones. Results were (positive/total cases): carcinoids (four of ten), nonsecreting paragangliomas (three of three), sporadic medullary carcinomas of the thyroid (MCT) (one of five), familial MCT (one of 26), chemodectomas (two of five), oat cell carcinomas (zero of four), choriocarcinoma (one of one), atypical schwannoma (with storage granules) (one of one), Merkel cell skin cancer (one of one), islet cell carcinoma (zero of one). We conclude that a wide range of neuroendocrine tumors show [131I]MIBG uptake; tumors other than pheochromocytomas and neuroblastomas are less often seen scintigraphically, but in certain cases (e.g., carcinoid and nonsecreting paragangliomas) scintigraphy may be useful in depicting the extent and location of disease and may indicate therapeutic potential. Iodine-131 MIBG shows promise in the diagnosis and staging of tumors of varied types.