RESUMEN

We have cloned the Hansenula polymorpha BIP gene from genomic DNA using a PCR-based strategy. H. polymorpha BIP encodes a protein of 665 amino acids, which shows very high homology to Saccharomvces cerevisiae KAR2p. KAR2p belongs to the Hsp70 family of molecular chaperones and resides in the endoplasmic reticulum (ER)-lumen. H. polymorpha BiP contains a putative N-terminal signal sequence of 30 amino acids together with the conserved -HDEL sequence, the typical ER retention signal, at the extreme C-terminus. We have analysed the effect of BIP overexpression, placing the gene under control of the strong alcohol oxidase promoter (P(MOX)) on the secretion of artificially produced Aspergillus niger glucose oxidase (GOX) by H. polymorpha. BiP overproduction did not lead to any growth defects of the cells; at the subcellular level, proliferation of ER-like vesicles was observed. However, artificially enhanced BiP levels strongly affected GOX secretion and led to accumulation of this protein in the ER-like vesicles. This was not simply due to the high BiP overproduction, because it was also observed under conditions of low P(MOX) induction during growth of cells on glycerol. Vacuolar carboxypeptidase Y was properly sorted to its target organelle in the BiP overproducing strains.

Asunto(s)

Aspergillus niger/enzimología , Retículo Endoplásmico/metabolismo , Proteínas Fúngicas/metabolismo , Glucosa Oxidasa/metabolismo , Proteínas HSP70 de Choque Térmico/metabolismo , Pichia/metabolismo , Secuencia de Aminoácidos , Clonación Molecular , Proteínas Fúngicas/genética , Proteínas HSP70 de Choque Térmico/genética , Proteínas de Choque Térmico/fisiología , Datos de Secuencia Molecular , Pichia/genética , Proteínas Recombinantes/biosíntesis , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Homología de Secuencia de Aminoácido

RESUMEN

We have analyzed the function of Hansenula polymorpha Pex14p in selective peroxisome degradation. Previously, we showed that Pex14p was involved in peroxisome biogenesis and functions in peroxisome matrix protein import. Evidence for the additional function of HpPex14p in selective peroxisome degradation (pexophagy) came from cells defective in HpPex14p synthesis. The suggestion that the absence of HpPex14p interfered with pexophagy was further analyzed by mutational analysis. These studies indicated that deletions at the C terminus of up to 124 amino acids of HpPex14p did not affect peroxisome degradation. Conversely, short deletions of the N terminus (31 and 64 amino acids, respectively) of the protein fully impaired pexophagy. Peroxisomes present in these cells remained intact for at least 6 h of incubation in the presence of excess glucose, conditions that led to the rapid turnover of the organelles in wild-type control cells. We conclude that the N terminus of HpPex14p contains essential information to control pexophagy in H. polymorpha and thus, that organelle development and turnover converge at Pex14p.

Asunto(s)

Proteínas Portadoras/biosíntesis , Proteínas Portadoras/genética , Proteínas de la Membrana/biosíntesis , Proteínas de la Membrana/genética , Peroxisomas/química , Peroxisomas/metabolismo , Proteínas Represoras , Secuencia de Aminoácidos , Aminoácidos/química , Western Blotting , Proteínas Fúngicas/metabolismo , Glucosa/metabolismo , Inmunohistoquímica , Proteínas de Transporte de Membrana , Microscopía Electrónica , Datos de Secuencia Molecular , Mutación , Peroxinas , Fosforilación , Pichia/química , Plásmidos/metabolismo , Estructura Terciaria de Proteína , Proteínas de Saccharomyces cerevisiae , Homología de Secuencia de Aminoácido , Factores de Tiempo

RESUMEN

Using spectroscopic techniques we studied the effect of the nucleophilic reagents cyanide, cyanate and thiocyanate on three flavo-oxidases namely alcohol oxidase (AO), glucose oxidase (GOX) and D-amino acid oxidase (DAOX). All three ions, added at concentrations in the mM range, caused release of the flavin adenine dinucleotide (FAD) co-factors from the enzyme molecules. In the case of AO this was accompanied by significant conformational perturbations, which was not observed for GOX and DAOX. As suggested from fluorescence, absorption and circular dichroism spectral changes at least one phenolic hydroxyl group became ionized upon FAD release from AO and a new class of Trp residues, fluorescent only in apo-AO protein, was demasked.

Asunto(s)

Flavina-Adenina Dinucleótido/química , Flavoproteínas/química , Oxidorreductasas/química , Oxidorreductasas de Alcohol/química , Dicroismo Circular , Cianatos , Cianuros , D-Aminoácido Oxidasa/química , Glucosa Oxidasa/química , Indicadores y Reactivos , Espectrometría de Fluorescencia , Espectrofotometría , Tiocianatos

RESUMEN

In a mutant form of Neurospora crassa, in which sheltered RIP (repeat induced point mutation) was used to deplete Tom19, protein transport through the TOM/TIM pathway is arrested by the addition of p-fluorophenylalanine (FPA). Using intermediate-voltage electron tomography, we have generated three-dimensional reconstructions of 28 FPA-treated mitochondria at four time points (0-32 h) after the addition of FPA. We determined that the cristae surface area and volume were lost in a roughly linear manner. A decrease in mitochondrial volume was not observed until after 16 h of FPA treatment. The inner boundary membrane did not appear to shrink or contract away from the outer membrane. Interestingly, the close apposition of these membranes remained over the entire periphery, even after all of the cristae had disappeared. The different dynamics of the shrinkage of cristae membrane and inner boundary membrane has implications for compartmentalization of electron transport proteins. Two structurally distinct types of contact sites were observed, consistent with recently published work. We determined that the cristae in the untreated (control) mitochondria are all lamellar. The cristae of FPA-treated mitochondria retain the lamellar morphology as they reduce in size and do not adopt tubular shapes. Importantly, the crista junctions exhibit tubular as well as slot-like connections to the inner boundary membrane, persisting until the cristae disappear, indicating that their stability is not dependent on continuous protein import through the complex containing Tom19.

Asunto(s)

Proteínas Fúngicas , Mitocondrias/ultraestructura , Receptores Citoplasmáticos y Nucleares/metabolismo , Mitocondrias/metabolismo , Neurospora crassa , Tomografía Computarizada por Rayos X

RESUMEN

In the methylotrophic yeast Hansenula polymorpha non-selective autophagy, induced by nitrogen starvation, results in the turnover of cytoplasmic components, including peroxisomes. We show that the uptake of these components occurs by invagination of the vacuolar membrane without their prior sequestration and thus differs from the mechanism described for bakers yeast. A selective mode of autophagy in H. polymorpha, namely glucose-induced peroxisome degradation, involves sequestration of individual peroxisomes tagged for degradation by membrane layers that subsequently fuse with the vacuole where the organelle is digested. H. polymorpha pdd mutants are blocked in selective peroxisome degradation. We observed that pdd1-201 is also impaired in non-selective autophagy, whereas this process still normally functions in pdd2-4. These findings suggest that mechanistically distinct processes as selective and non-selective autophagy involve common but also unique genes.

Asunto(s)

Proteínas Fúngicas/metabolismo , Regulación Fúngica de la Expresión Génica , Genes Fúngicos , Glucosa/metabolismo , Nitrógeno/metabolismo , Peroxisomas/metabolismo , Pichia/metabolismo , Autofagia , Proteínas Fúngicas/genética , Metanol/metabolismo , Microscopía Electrónica , Pichia/genética , Pichia/crecimiento & desarrollo

RESUMEN

We have analyzed the properties of peroxisomal remnants in Hansenula polymorpha pex5 cells. In such cells PTS1 matrix protein import is fully impaired. In H. polymorpha pex5 cells, grown on ethanol/ammonium sulfate, conditions that repressed the PTS2 protein amine oxidase (AMO), peroxisomal structures were below the limit of detection. In methanol/ammonium sulfate-grown cells, normal peroxisomes are absent, but a few small membranous structures were observed that apparently represented peroxisomal ghosts since they contained Pex14p. These structures were the target of a Pex10p.myc fusion protein that was produced in pex5 cells under the control of the homologous alcohol oxidase promoter (strain pex5::P(AOX).PEX10.MYC). Glycerol/methanol/ammonium sulfate-grown cells of this transformant were placed in fresh glucose/methylamine media, conditions that fully repress the synthesis of the Pex10p.myc fusion protein but induce the synthesis of AMO. Two hours after the shift Pex10p.myc-containing structures were detectable that had accumulated newly synthesized AMO protein and which during further cultivation developed in normal peroxisomes. Concurrently, the remaining portion of these structures was rapidly degraded. These findings indicate that peroxisomal remnants in pex5 cells can develop into peroxisomes. Also, as for normal peroxisomes in H. polymorpha, apparently a minor portion of these structures actually take part in the development of these organelles.

Asunto(s)

Amina Oxidasa (conteniendo Cobre)/biosíntesis , Peroxisomas , Pichia/ultraestructura , Receptores Citoplasmáticos y Nucleares/biosíntesis , Receptores Citoplasmáticos y Nucleares/genética , Secuencia de Bases , Cartilla de ADN , Inducción Enzimática , Proteínas Fúngicas , Inmunohistoquímica , Microscopía Electrónica , Receptor de la Señal 2 de Direccionamiento al Peroxisoma , Receptor de la Señal 1 de Direccionamiento al Peroxisoma , Pichia/enzimología , Pichia/genética

RESUMEN

The Saccharomyces cerevisiae ICL1 gene encodes isocitrate lyase, an essential enzyme for growth on ethanol and acetate. Previous studies have demonstrated that the highly homologous ICL2 gene (YPR006c) is transcribed during the growth of wild-type cells on ethanol. However, even when multiple copies are introduced, ICL2 cannot complement the growth defect of icl1 null mutants. It has therefore been suggested that ICL2 encodes a nonsense mRNA or nonfunctional protein. In the methylcitrate cycle of propionyl-coenzyme A metabolism, 2-methylisocitrate is converted to succinate and pyruvate, a reaction similar to that catalyzed by isocitrate lyase. To investigate whether ICL2 encodes a specific 2-methylisocitrate lyase, isocitrate lyase and 2-methylisocitrate lyase activities were assayed in cell extracts of wild-type S. cerevisiae and of isogenic icl1, icl2, and icl1 icl2 null mutants. Isocitrate lyase activity was absent in icl1 and icl1 icl2 null mutants, whereas in contrast, 2-methylisocitrate lyase activity was detected in the wild type and single icl mutants but not in the icl1 icl2 mutant. This demonstrated that ICL2 encodes a specific 2-methylisocitrate lyase and that the ICL1-encoded isocitrate lyase exhibits a low but significant activity with 2-methylisocitrate. Subcellular fractionation studies and experiments with an ICL2-green fluorescent protein fusion demonstrated that the ICL2-encoded 2-methylisocitrate lyase is located in the mitochondrial matrix. Similar to that of ICL1, transcription of ICL2 is subject to glucose catabolite repression. In glucose-limited cultures, growth with threonine as a nitrogen source resulted in a ca. threefold induction of ICL2 mRNA levels and of 2-methylisocitrate lyase activity in cell extracts relative to cultures grown with ammonia as the nitrogen source. This is consistent with an involvement of the 2-methylcitrate cycle in threonine catabolism.

Asunto(s)

Acilcoenzima A/metabolismo , Liasas de Carbono-Carbono/genética , Liasas de Carbono-Carbono/metabolismo , Isocitratoliasa/genética , Isocitratoliasa/metabolismo , Mitocondrias/enzimología , Saccharomyces cerevisiae/enzimología , Medios de Cultivo , Regulación Fúngica de la Expresión Génica , Genes Fúngicos , Glucosa/farmacología , Saccharomyces cerevisiae/genética , Análisis de Secuencia de ADN , Fracciones Subcelulares , Treonina/farmacología

RESUMEN

The development of heterologous overexpression systems for soluble proteins has greatly advanced the study of the structure/function relationships of these proteins and their biotechnological and pharmaceutical applications. In this paper we present an overview on several aspects of the use of the methylotrophic yeast Hansenula polymorpha as a host for heterologous gene expression. H. polymorpha has been successfully exploited as a cell factory for the large-scale production of such components. Stable, engineered strains can be obtained by site-directed integration of expression cassettes into the genome, for which various constitutive and inducible promoters are available to control the expression of the foreign genes. New developments have now opened the way to additional applications of H. polymorpha, which are unprecedented for other organisms. Most importantly, it may be the organism of choice for reliable, large-scale production of heterologous membrane proteins, using inducible intracellular membranes and targeting sequences to specifically insert these proteins stably into these membranes. Furthermore, the use of H. polymorpha offers the possibility to accumulate the produced components into specific compartments, namely peroxisomes. These organelles are massively induced during growth of the organism on methanol and may occupy up to 80% of the cell volume. Accumulation inside peroxisomes prevents undesired modifications (e.g. proteolytic processing or glycosylation) and is also in particular advantageous when proteins are produced which are toxic or harmful for the host.

RESUMEN

Peroxisomes are subcellular organelles and are present in virtually all eukaryotic cells. Characteristic features of these organelles are their inducibility and their functional versatility. Their importance in the intermediary metabolism of cells is exemplified by the discovery of several inborn, fatal peroxisomal errors in man, the so-called peroxisomal disorders. Recent findings in research on peroxisome biogenesis and function have demonstrated that peroxisomal matrix proteins and peroxisomal membrane proteins (PMPs) follow separate pathways to reach their target organelle. This paper addresses the principles of PMP sorting and summarizes the current knowledge of the role of these proteins in organelle biogenesis and function.

Asunto(s)

Proteínas de la Membrana/metabolismo , Peroxisomas/metabolismo , Animales , Humanos , Procesamiento Proteico-Postraduccional

RESUMEN

Hansenula polymorpha Deltapex14 cells are affected in peroxisomal matrix protein import and lack normal peroxisomes. Instead, they contain peroxisomal membrane remnants, which harbor a very small amount of the major peroxisomal matrix enzymes alcohol oxidase (AO) and dihydroxyacetone synthase (DHAS). The bulk of these proteins is, however, mislocated in the cytosol. Here, we show that in Deltapex14 cells overproduction of the PTS1 receptor, Pex5p, leads to enhanced import of the PTS1 proteins AO and DHAS but not of the PTS2 protein amine oxidase. The import of the PTS1 protein catalase (CAT) was not stimulated by Pex5p overproduction. The difference in import behavior of AO and CAT was not related to their PTS1, since green fluorescent protein fused to the PTS1 of either AO or CAT were both not imported in Deltapex14 cells overproducing Pex5p. When produced in a wild type control strain, both proteins were normally imported into peroxisomes. In Deltapex14 cells overproducing Pex5p, Pex5p had a dual location and was localized in the cytosol and bound to the outer surface of the peroxisomal membrane. Our results indicate that binding of Pex5p to the peroxisomal membrane and import of certain PTS1 proteins can proceed in the absence of Pex14p.

Asunto(s)

Oxidorreductasas de Alcohol/metabolismo , Transferasas de Aldehído-Cetona/metabolismo , Proteínas Portadoras , Proteínas Fúngicas/fisiología , Proteínas de la Membrana/fisiología , Receptores Citoplasmáticos y Nucleares/fisiología , Proteínas Represoras , Western Blotting , Endopeptidasas/metabolismo , Proteínas Fúngicas/genética , Glicerol/metabolismo , Inmunohistoquímica , Proteínas de la Membrana/genética , Proteínas de Transporte de Membrana , Metanol/farmacología , Microscopía Electrónica , Modelos Biológicos , Mutagénesis , Peroxinas , Receptor de la Señal 1 de Direccionamiento al Peroxisoma , Pichia/citología , Pichia/genética , Receptores Citoplasmáticos y Nucleares/genética , Receptores Citoplasmáticos y Nucleares/metabolismo , Proteínas de Saccharomyces cerevisiae , Sacarosa/metabolismo

RESUMEN

Pex3p is a peroxisomal membrane protein that is essential for peroxisome biogenesis. Here, we show that a conserved stretch of positively charged amino acids (Arg(11)-X-Lys-Lys-Lys(15)) in the N terminus of Hansenula polymorpha Pex3p is involved in incorporation of the protein into its target membrane. Despite the strong conservation, this sequence shows a high degree of redundancy. Substitution of either Arg(11), Lys(13), Lys(14), or Lys(15) with uncharged or negatively charged amino acids did not interfere with Pex3p location and function. However, a mutant Pex3p, carrying negatively charged amino acids at position 13 and 15 (K13E/K15E), caused moderate but significant defects in peroxisome assembly and matrix protein import. Additional changes in the N terminus of Pex3p, e.g. replacing three or four of the positively charged amino acids with negatively charged ones, led to a typical pex3 phenotype, i.e. accumulation of peroxisomal matrix proteins in the cytosol and absence of peroxisomal remnants. Also, in these cases, the mutant Pex3p levels were reduced. Remarkably, mutant Pex3p proteins were mislocalized to mitochondria or the cytosol, depending on the nature of the mutation. Furthermore, in case of reduced amounts of Pex3p, the levels of other peroxisomal membrane proteins, e.g. Pex10p and Pex14p, were also diminished, suggesting that Pex3p maybe involved in the recruitment or stabilization of these proteins (in the membrane).

Asunto(s)

Transportadoras de Casetes de Unión a ATP , Proteínas Fúngicas/química , Proteínas Fúngicas/metabolismo , Membranas Intracelulares/metabolismo , Proteínas de la Membrana/química , Proteínas de la Membrana/metabolismo , Peroxisomas/metabolismo , Pichia/metabolismo , Proteínas de Saccharomyces cerevisiae , Secuencia de Aminoácidos , Sustitución de Aminoácidos , Animales , Secuencia de Consenso , Secuencia Conservada , Cartilla de ADN , Proteínas Fúngicas/genética , Proteínas Fluorescentes Verdes , Membranas Intracelulares/ultraestructura , Proteínas Luminiscentes/análisis , Proteínas Luminiscentes/genética , Proteínas de la Membrana/genética , Datos de Secuencia Molecular , Mutagénesis Sitio-Dirigida , Peroxinas , Peroxisomas/ultraestructura , Pichia/genética , Pichia/crecimiento & desarrollo , Plásmidos , Proteínas Recombinantes de Fusión/biosíntesis , Proteínas Recombinantes/química , Proteínas Recombinantes/metabolismo , Alineación de Secuencia , Eliminación de Secuencia , Homología de Secuencia de Aminoácido

RESUMEN

In yeast, peroxisomes are the site of specific catabolic pathways that characteristically include hydrogen peroxide producing oxidases and catalase. During the last 10 years, much progress has been made in unravelling the molecular mechanisms involved in the biogenesis of this organelle. At present, 23 different genes (PEX genes) have been identified that are involved in different aspects of peroxisome biogenesis (e.g., proliferation, formation of the peroxisomal membrane, import of matrix proteins). The principles of peroxisome degradation are still much less understood. Recently, the first yeast mutants affected in this process have become available and used to clone corresponding genes by functional complementation. In this paper, an overview is presented of the research on yeast peroxisomes, focusing on recent achievements in the molecular aspects of peroxisome development, function, and turnover.

Asunto(s)

Peroxisomas/fisiología , Peroxisomas/ultraestructura , Levaduras/fisiología , Endopeptidasa Neutra Reguladora de Fosfato PHEX , Proteínas/genética , Proteínas/metabolismo , Levaduras/ultraestructura

RESUMEN

Methylotrophic yeasts contain large peroxisomes during growth on methanol. Upon exposure to excess glucose or ethanol these organelles are selectively degraded by autophagy. Here we describe the cloning of a Pichia pastoris gene (PpVPS15) involved in peroxisome degradation, which is homologous to Saccharomyces cerevisiae VPS15. In methanol-grown cells of a P. pastoris VPS15 deletion strain, the levels of peroxisomal marker enzymes remained high after addition of excess glucose or ethanol. Electron microscopic studies revealed that the organelles were not taken up by vacuoles, suggesting that PpVPS15 is required at an early stage in peroxisome degradation.

Asunto(s)

Genes Fúngicos , Peroxisomas/ultraestructura , Pichia/genética , Pichia/ultraestructura , Proteínas Serina-Treonina Quinasas/genética , Secuencia de Aminoácidos , Autofagia/efectos de los fármacos , Autofagia/genética , Secuencia de Bases , Clonación Molecular , Cartilla de ADN/genética , Complejos de Clasificación Endosomal Requeridos para el Transporte , Etanol/farmacología , Eliminación de Gen , Glucosa/farmacología , Microscopía Inmunoelectrónica , Datos de Secuencia Molecular , Peroxisomas/efectos de los fármacos , Pichia/efectos de los fármacos , Pichia/fisiología , Proteínas Serina-Treonina Quinasas/fisiología , Saccharomyces cerevisiae/genética , Proteínas de Saccharomyces cerevisiae , Homología de Secuencia de Aminoácido , Especificidad de la Especie , Proteína de Clasificación Vacuolar VPS15

RESUMEN

We have cloned the Hansenula polymorpha PEX1 and PEX6 genes by functional complementation of the corresponding peroxisome-deficient (pex) mutants. The gene products, HpPex1p and HpPex6p, are ATPases which both belong to the AAA protein family. Cells deleted for either gene (Deltapex1 or Deltapex6) were characterized by the presence of small peroxisomal remnants which contained peroxisomal membrane proteins and minor amounts of matrix proteins. The bulk of the matrix proteins, however, resided in the cytosol. In cell fractionation studies HpPex1p and HpPex6p co-sedimented with the peroxisomal membrane protein HpPex3p in both wild-type cells and in Deltapex4, Deltapex8 or Deltapex14 cells. Both proteins are loosely membrane-bound and face the cytosol. Furthermore, HpPex1p and HpPex6p physically and functionally interact in vivo. Overexpression of PEX6 resulted in defects in peroxisomal matrix protein import. By contrast, overexpression of PEX1 was not detrimental to the cells. Interestingly, co-overproduction of HpPex1p rescued the protein import defect caused by HpPex6p overproduction. Overproduced HpPex1p and HpPex6p remained predominantly membrane-bound, but only partially co-localized with the peroxisomal membrane protein HpPex3p. Our data indicate that HpPex1p and HpPex6p function in a protein complex associated with the peroxisomal membrane and that overproduced, mislocalized HpPex6p prevents HpPex1p from reaching its site of activity.

Asunto(s)

Adenosina Trifosfatasas/genética , Microcuerpos/fisiología , Pichia/fisiología , Secuencia de Aminoácidos , Animales , Anticuerpos Antifúngicos/biosíntesis , Secuencia de Bases , Southern Blotting , Western Blotting , Clonación Molecular , Cartilla de ADN/química , ADN de Hongos/química , Electroforesis en Gel de Poliacrilamida , Electroporación , Inmunohistoquímica , Microcuerpos/genética , Microcuerpos/ultraestructura , Microscopía Electrónica , Datos de Secuencia Molecular , Mutación , Pichia/genética , Pichia/ultraestructura , Reacción en Cadena de la Polimerasa , Pruebas de Precipitina , Conejos , Alineación de Secuencia , Análisis de Secuencia de ADN , Homología de Secuencia de Aminoácido

RESUMEN

Via functional complementation we have isolated the Hansenula polymorpha PDD1 gene essential for selective, macroautophagic peroxisome degradation. HpPDD1 encodes a 116 kDa protein with high similarity (42% identity) to Saccharomyces cerevisiae Vps34p, which has been implicated in vacuolar protein sorting and endocytosis. Western blotting experiments revealed that HpPDD1 is expressed constitutively. In a H. polymorpha pdd1 disruption strain peroxisome degradation is fully impaired. Sequestered peroxisomes, typical for the first stage of peroxisome degradation in H. polymorpha, were never observed, suggesting that HpPdd1p plays a role in the tagging of redundant peroxisomes and/or sequestration of these organelles from the cytosol. Possibly, HpPdd1p is the functional homologue of ScVps34p, because-like S. cerevisiae vps34 mutants-H. polymorpha pdd1 mutants are temperature-sensitive for growth and are impaired in the sorting of vacuolar carboxypeptidase Y. Moreover, HpPdd1p is associated to membranes, as was also observed for ScVps34p.

Asunto(s)

Proteínas Fúngicas/metabolismo , Microcuerpos/metabolismo , Fosfatidilinositol 3-Quinasas/genética , Pichia/genética , Saccharomyces cerevisiae/genética , Oxidorreductasas de Alcohol/metabolismo , Secuencia de Aminoácidos , Transporte Biológico , Carboxipeptidasas/genética , Carboxipeptidasas/metabolismo , Catepsina A , Fraccionamiento Celular , Membrana Celular/enzimología , Membrana Celular/ultraestructura , Clonación Molecular , Proteínas Fúngicas/química , Proteínas Fúngicas/genética , Eliminación de Gen , Prueba de Complementación Genética , Proteínas de la Membrana/química , Proteínas de la Membrana/genética , Proteínas de la Membrana/metabolismo , Microcuerpos/ultraestructura , Datos de Secuencia Molecular , Mutagénesis Insercional , Sistemas de Lectura Abierta/genética , Fosfatidilinositol 3-Quinasas/química , Pichia/enzimología , Pichia/metabolismo , Pichia/ultraestructura , Saccharomyces cerevisiae/enzimología , Homología de Secuencia de Aminoácido , Vacuolas/enzimología , Vacuolas/ultraestructura

RESUMEN

We have shown that peroxisomes of the yeast Yarrowia lipolytica are subject to specific degradation after exposure of acetate/oleate-grown cells to glucose excess conditions. Electron microscopic analysis has revealed that the peroxisomes were degraded by uptake in the vacuole. Our results suggest that peroxisomes are taken up by macroautophagic processes, because sequestration of individual peroxisomes, which occurs typically at the beginning of microautophagy, was never observed. The observation that a peroxisomal amine oxidase activity is specifically induced by ethylamine was used for the development of a plate assay screening procedure to isolate peroxisome degradation-defective mutants.

Asunto(s)

Microcuerpos/metabolismo , Saccharomycetales/metabolismo , Acetatos/metabolismo , Amina Oxidasa (conteniendo Cobre)/metabolismo , Sulfato de Amonio/metabolismo , Medios de Cultivo , Etilaminas/metabolismo , Glucosa/metabolismo , Mutación , Ácido Oléico/metabolismo , Saccharomycetales/genética , Saccharomycetales/crecimiento & desarrollo

RESUMEN

Alcohol oxidase (AO) is a homo-octameric flavoenzyme which catalyzes methanol oxidation in methylotrophic yeasts. AO protein is synthesized in the cytosol and subsequently sorted to peroxisomes where the active enzyme is formed. To gain further insight in the molecular mechanisms involved in AO activation, we studied spectroscopically native AO from Hansenula polymorpha and Pichia pastoris and three putative assembly intermediates. Fluorescence studies revealed that both Trp and FAD are suitable intramolecular markers of the conformation and oligomeric state of AO. A direct relationship between dissociation of AO octamers and increase in Trp fluorescence quantum yield and average fluorescence lifetime was found. The time-resolved fluorescence of the FAD cofactor showed a rapid decay component which reflects dynamic quenching due to the presence of aromatic amino acids in the FAD-binding pocket. The analysis of FAD fluorescence lifetime profiles showed a remarkable resemblance of pattern for purified AO and AO present in intact yeast cells. Native AO contains a high content of ordered secondary structure which was reduced upon FAD-removal. Dissociation of octamers into monomers resulted in a conversion of beta-sheets into alpha-helices. Our results are explained in relation to a 3D model of AO, which was built based on the crystallographic data of the homologous enzyme glucose oxidase from Aspergillus niger. The implications of our results for the current model of the in vivo AO assembly pathway are discussed.

Asunto(s)

Oxidorreductasas de Alcohol/química , Microcuerpos/enzimología , Pichia/enzimología , Dicroismo Circular , Flavina-Adenina Dinucleótido/química , Polarización de Fluorescencia , Modelos Moleculares , Conformación Proteica , Estructura Secundaria de Proteína , Homología de Secuencia de Aminoácido , Espectrometría de Fluorescencia , Triptófano/química

RESUMEN

We have isolated the Hansenula polymorpha CPY gene encoding carboxypeptidase Y (Hp-CPY). The deduced amino acid sequence revealed that Hp-CPY consists of 541 amino acids and has a calculated Mr of 60,793. The protein is highly similar to Saccharomyces cerevisiae CPY (61.8% identity). At the N-terminus of Hp-CPY signals for the entry into the secretory pathway and subsequent sorting to the vacuole were identified. Immunocytochemically, using monospecific antibodies raised against Hp-CPY, the protein was localized to the vacuole. On Western blots, a diffuse protein band was observed in extracts of H. polymorpha cells, suggesting that the protein is glycosylated. This was confirmed by endoglycosidase H treatment, which resulted in a strong reduction of the apparent Mr of the protein. We have investigated the effect of CPY deletion on the degradation of peroxisomes, an autophagous process that occurs when the organelles become redundant for growth. In deltacpy cells peroxisomal proteins were degraded in the vacuole as efficiently as in wild-type H. polymorpha cells, indicating that CPY is not a major proteinase in this pathway.

Asunto(s)

Carboxipeptidasas/genética , Genes Fúngicos/genética , Pichia/genética , Secuencia de Aminoácidos , Western Blotting , Carboxipeptidasas/química , Carboxipeptidasas/metabolismo , Catepsina A , Clonación Molecular , Biblioteca Genómica , Glicósido Hidrolasas/metabolismo , Glicosilación , Inmunohistoquímica , Microcuerpos/metabolismo , Microcuerpos/fisiología , Datos de Secuencia Molecular , Peso Molecular , Mutagénesis Insercional , Sistemas de Lectura Abierta/genética , Pichia/citología , Pichia/enzimología , Pichia/crecimiento & desarrollo , Señales de Clasificación de Proteína/genética , Proteínas Recombinantes de Fusión/química , Proteínas Recombinantes de Fusión/metabolismo , Alineación de Secuencia , Eliminación de Secuencia , Homología de Secuencia de Ácido Nucleico , Vacuolas/metabolismo

RESUMEN

In previous work, we have demonstrated that oleate induces a massive proliferation of microbodies (peroxisomes) in Aspergillus nidulans. Although at a lower level, proliferation of peroxisomes also occurs in cells growing under conditions that induce penicillin biosynthesis. Here, microbodies in oleate-grown A. nidulans cells were characterized by using several antibodies that recognize peroxisomal enzymes and peroxins in a broad spectrum of eukaryotic organisms such as yeast, and plant, and mammalian cells. Peroxisomes were immunolabeled by anti-SKL and anti-thiolase antibodies, which suggests that A. nidulans conserves both PTS1 and PTS2 import mechanisms. Isocitrate lyase and malate synthase, the two key enzymes of the glyoxylate cycle, were also localized in these organelles. In contrast to reports of Neurospora crassa, our results demonstrate that A. nidulans contains only one type of microbody (peroxisomes) that carry out the glyoxylate cycle and contain 3-ketoacyl-CoA thiolase and proteins with the C-terminal SKL tripeptide.

Asunto(s)

Aspergillus nidulans/ultraestructura , Microcuerpos , Animales , Aspergillus nidulans/enzimología , Aspergillus nidulans/crecimiento & desarrollo , Western Blotting , Medios de Cultivo , Electroforesis en Gel de Poliacrilamida , Microcuerpos/enzimología , Microcuerpos/ultraestructura , Microscopía Inmunoelectrónica , Conejos

RESUMEN

The Hansenula polymorpha per6-210 mutant is impaired in respect of growth on methanol (Mut-) and is characterized by aberrant peroxisome formation. The functionally complementing DNA fragment contains two open reading frames. The first encodes dihydroxyacetone kinase (DAK), a cytosolic enzyme essential for formaldehyde assimilation; the second ORF codes for a novel protein (Pak1p). We have demonstrated that per6-210 cells lack DAK activity, causing the Mut- phenotype, and have strongly reduced levels of Pak1p, resulting in peroxisomal defects. Sequence analysis revealed that per6-210 contains a mutation in the 3' end of the DAK coding region, which overlaps with the promoter region of PAK1. Possibly this mutation also negatively affects PAK1 expression.

Asunto(s)

Proteínas Fúngicas/genética , Microcuerpos/genética , Mutación , Fosfotransferasas (Aceptor de Grupo Alcohol)/genética , Pichia/genética , Secuencia de Aminoácidos , Clonación Molecular , ADN Complementario , Proteínas Fúngicas/análisis , Proteínas Fúngicas/química , Espectroscopía de Resonancia Magnética , Metanol/metabolismo , Metanol/farmacología , Microcuerpos/química , Microscopía Electrónica , Datos de Secuencia Molecular , Neurospora crassa/genética , Sistemas de Lectura Abierta , Fosfotransferasas (Aceptor de Grupo Alcohol)/química , Regiones Promotoras Genéticas , Mapeo Restrictivo , Análisis de Secuencia , Homología de Secuencia