RESUMEN

In this work we explore a new hydrogel stamp material obtained from polymerizing 2-hydroxyethyl acrylate and poly(ethylene glycol) diacrylate in the presence of water for the microcontact printing of proteins directly on gold substrates and by covalent coupling to self-assembled monolayers of alkanethiols. At high cross-link density, the hydrogel is rigid, hydrophilic, and with a high buffer holding capacity to enable the unsupported printing of protein patterns homogeneously and reproducibly, with micrometer-range precision. The stamps were used to print antibodies to human parathyroid hormone, which were shown using immunoassay tests to retain their biological function with binding capacities comparable to those of solution-adsorbed antibodies.

Asunto(s)

Acrilatos/química , Hidrogel de Polietilenoglicol-Dimetacrilato/química , Hormona Paratiroidea/química , Polietilenglicoles/química , Albúmina Sérica Bovina/química , Acrilatos/síntesis química , Animales , Anticuerpos/química , Anticuerpos/inmunología , Bovinos , Ensayo de Inmunoadsorción Enzimática , Oro/química , Humanos , Hidrogel de Polietilenoglicol-Dimetacrilato/síntesis química , Interacciones Hidrofóbicas e Hidrofílicas , Hormona Paratiroidea/inmunología , Polietilenglicoles/síntesis química , Sensibilidad y Especificidad , Albúmina Sérica Bovina/inmunología , Espectrometría de Fluorescencia/instrumentación , Espectrometría de Fluorescencia/métodos , Compuestos de Sulfhidrilo/química , Propiedades de Superficie , Agua/química