RESUMEN

We report that Brucella abortus and Brucella melitensis agglutinate human (A+ and B+), hamster and rabbit erythrocytes, a heretofore undescribed feature in this genus. This activity was associated with a 29-kDa surface protein (SP29) that bound selectively to these erythrocytes and this binding was inhibited by rabbit anti-SP29 antibodies. Hemagglutination was inhibited by pretreatment of erythrocytes with neuraminidase and by preincubation of B. abortus with chondroitin sulfate, N-acetylneuraminic acid and N-acetylneuramin-lactose.

Asunto(s)

Adhesión Bacteriana , Brucella/inmunología , Eritrocitos/inmunología , Ácido N-Acetilneuramínico/metabolismo , Secuencia de Aminoácidos , Animales , Reacciones Antígeno-Anticuerpo , Brucella abortus/inmunología , Sulfatos de Condroitina/farmacología , Cricetinae , Eritrocitos/efectos de los fármacos , Eritrocitos/metabolismo , Pruebas de Hemaglutinación , Humanos , Técnicas In Vitro , Datos de Secuencia Molecular , Neuraminidasa/farmacología , Conejos , Receptores de Superficie Celular/metabolismo