RESUMEN
Antibody titres to EBV-associated antigens in Chinese NPC patients were analysed according to length of survival after diagnosis and to disease stage. Geometric mean titres of ADLC antibody were highest in the long-term survivors, whereas VCA, EA and EBNA antibody titres showed an inverse relationship to survival. High VCA, EA and EBNA titres were less frequent, and high ADLC titres were more frequent in long-term survivors than in intermediate or short-term survivors. The association of geometric mean titres of EBV antibodies with prognosis could not be entirely explained by stage of disease. A functional role for the ADLC antibody is suggested by the association of a high ADLC antibody titre with a good prognosis regardless of stage of disease.
Asunto(s)
Anticuerpos Antivirales/análisis , Citotoxicidad Celular Dependiente de Anticuerpos , Herpesvirus Humano 4/inmunología , Linfocitos/inmunología , Neoplasias Nasofaríngeas/inmunología , Antígenos Virales , China/etnología , Humanos , Neoplasias Nasofaríngeas/microbiología , Neoplasias Nasofaríngeas/mortalidad , PronósticoRESUMEN
Delayed hypersensibility to antigens derived from four lymphoid cell lines was measured in 27 non-Chinese patients with nasopharyngeal cancer (NPC) and 63 non-NPC cancer patients. Of the NPC patients, 17/27 (63%) had a positive skin test response to antigens derived from HKLY-28, a lymphoid cell line which was developed from an NPC biopsy. Only 10/51 (20%) and 1/13 (8%) patients with solid tumors and hematopoietic malignancies, respectively, had positive skin test responses to HKLY-28. Positive skin tests were found less frequently when extracts from cell lines derived from normal individuals or lymphoma patients were utilized, although NPC patients were more reactive to two of the non-NPC derived cell lines than the controls. The NPC patients in this study also had significantly elevated antibody titers to the Epstein-Barr virus (EBV), capsid antigen (VCA) and early antigen (EA). Titers were highest in the patients with more anaplastic nasopharyngeal carcinomas. The skin test and serologic data are consistent with studies in Chinese patients, indicating that NPC in non-Chinese and Chinese patients is biologically similar.
Asunto(s)
Antígenos de Neoplasias , Carcinoma de Células Escamosas/inmunología , Carcinoma/inmunología , Neoplasias Nasofaríngeas/inmunología , Adulto , África del Norte , Anciano , Anticuerpos Antivirales/análisis , Pueblo Asiatico , Línea Celular , Niño , China , Europa (Continente) , Femenino , Herpesvirus Humano 4/inmunología , Historia del Siglo XVIII , Humanos , Hipersensibilidad Tardía , Masculino , Persona de Mediana Edad , Grupos Raciales , Pruebas CutáneasAsunto(s)
Antígenos Virales/aislamiento & purificación , Linfoma de Burkitt/inmunología , Herpesvirus Humano 4/inmunología , Adolescente , Adulto , Anticuerpos Antivirales/aislamiento & purificación , Reacciones Antígeno-Anticuerpo , Niño , Preescolar , Pruebas de Fijación del Complemento , Epítopos , Femenino , Humanos , Lactante , Masculino , Persona de Mediana Edad , Estadística como Asunto , Estados UnidosRESUMEN
A comparative study of the extent of Epstein-Barr virus (EBV) infections in populations that differ with respect to the incidence of tumours associated with this virus is now in progress in different countries. In these surveys of antibody titres from the various study populations, it is of critical importance that strict comparability be maintained. Despite standardization of techniques and reagents in the cooperating laboratories, considerable variation in the results has remained. The components of the total variability in the results of the immunofluorescence test for estimating the antibody titres against viral capsid antigens (VCA) of the EBV have been investigated. With repeated tests on the same sera, four sources of variation were measured: the reading of the slides, the performance of the tests, the use of various batches of the same cell line as antigen, and the use of different cell lines. The greatest variations were due to the use of different cell lines and to differences in performing the test; the reading of the slides caused only minor variations. Both the systematic and unsystematic variations were measured. The systematic variation was great in tests between laboratories and when different cell lines were used as antigens. Most of the systematic variation resulting from the use of different cell batches from the same cell line could be accounted for by the differing proportions of brilliant fluorescent cells. Adjustments are possible to correct the systematic variation whenever this has been measured, but not the unsystematic "residual" variability, which presents the real obstacle to the comparison of results obtained in different laboratories or by different observers. To attain full comparability of VCA antibody tests the sera from the different surveys should all be tested in the same laboratory.