RESUMEN
Co-fermentation and simultaneous saccharification of rice hull hydrolysate (RHH) were investigated for the production of ethanol and xylitol by Saccharomyces cerevisiae, Spathaspora arborariae, or the combination of both. In bioreactor cultures under oxygen limitation, S. cerevisiae was capable of metabolizing glucose from RHH, which contained small amounts of acetic acid, furfural, and hydroxymethylfurfural, achieving ethanol yields of 0.45 and concentrations of 10.5 g L(-1). In the co-culture of S. cerevisiae and S. arborariae pentoses and hexoses from RHH, were converted to ethanol and xylitol, with yields of 0.48 and 0.39, and concentrations of 11 g L(-1) and 3 g L(-1), respectively. The simultaneous saccharification and co-fermentation using both yeasts produced ethanol and xylitol to final concentrations of 14.5 g L(-1) and 3 g L(-1), respectively. Results showed good prospects to use co-cultures of S. cerevisiae and S. arborariae for the bioconversion of RHH into ethanol and xylitol without further detoxification.
Asunto(s)
Carbohidratos/química , Etanol/metabolismo , Fermentación , Oryza/metabolismo , Saccharomycetales/metabolismo , Xilitol/biosíntesis , Hidrólisis , Cinética , Saccharomycetales/clasificaciónRESUMEN
The ability of Candida shehatae, Saccharomyces cerevisiae, or the combination of these two yeasts in converting the mixed sugar composition of rice hull hydrolysate (RHH) as substrate for ethanol production is presented. In shake flask experiments, co-cultures showed ethanol yields (YP/S) of 0.42 and 0.51 in synthetic medium simulating the sugar composition of RHH and in RHH, respectively, with both glucose and xylose being completely depleted, while pure cultures of C. shehatae produced slightly lower ethanol yields (0.40). Experiments were scaled-up to bioreactors, in which anaerobiosis and oxygen limitation conditions were tested. Bioreactor co-cultures produced similar ethanol yields in both conditions (0.50-0.51) in synthetic medium, while in RHH, yields of 0.48 and 0.44 were obtained, respectively. The results showed near-theoretical yields of ethanol. Results suggest the feasibility of co-cultures of C. shehatae, a newly isolated strain, and S. cerevisiae in RHH as substrate for second-generation ethanol production.
Asunto(s)
Candida/metabolismo , Etanol/metabolismo , Glucosa/metabolismo , Oryza/microbiología , Saccharomyces cerevisiae/metabolismo , Semillas/microbiología , Xilosa/metabolismo , Candida/clasificación , Fraccionamiento Celular , Técnicas de Cocultivo/métodos , Etanol/aislamiento & purificación , Estudios de Factibilidad , Hidrólisis , Saccharomyces cerevisiae/clasificación , Especificidad de la EspecieRESUMEN
The production of ethanol by the new yeast Spathaspora arborariae using rice hull hydrolysate (RHH) as substrate, either alone or in co-cultures with Saccharomyces cerevisiae is presented. Cultivations were also carried out in synthetic medium to gather physiological information on these systems, especially concerning their ability to grow and produce ethanol in the presence of acetic acid, furfural, and hydroxymethylfurfural, which are toxic compounds usually present in lignocellulosic hydrolysates. S. arborariae was able to metabolize xilose and glucose present in the hydrolysate, with ethanol yields (Y(P/S)(et)) of 0.45. In co-cultures, ethanol yields peaked to 0.77 and 0.62 in the synthetic medium and in RHH, respectively. When the toxic compounds were added to the synthetic medium, their presence produced negative effects on biomass formation and ethanol productivity. This work shows good prospects for the use of the new yeast S. arborariae alone and in co-cultures with S. cerevisiae for ethanol production.