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BACKGROUND: TB is an infectious disease with a worldwide impact. TB is closely associated with social and housing conditions, exerting a significant impact on the prison population, which is particularly susceptible to the disease. Evidence suggests that the COVID-19 pandemic has exacerbated social vulnerability. This study therefore aimed to analyse the impact of the pandemic on the diagnosis of new cases of TB in the Brazilian prison population in the years 2020 and 2021.METHODS: This is an ecological study involving cases of TB recorded in the prison population of Brazil from 2015 to 2021. Data were collected from the Brazil's Information System for Notifiable Diseases.RESULTS: The incidence of TB in the prison population in Brazil fell from 1,005.9/100,000 population between 2015 and 2019 to 852.3/100,000 population between 2020 and 2021. In 2021, there was a deficit of 539 cases (-7.6%) compared to what was expected for the year. In 2020, there was a 10% reduction in TB diagnoses in January and February, reaching 3.8% in March. A negative percentage was observed in most of the subsequent months. In 2021, the year began with a 21.6% decline in January, returning to positive values only in August and September.CONCLUSION: The first years of the COVID-19 pandemic resulted in underdiagnosis of TB in the Brazilian prison population.
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COVID-19 , Pandemias , Prisioneros , Tuberculosis , COVID-19/epidemiología , COVID-19/prevención & control , Pandemias/prevención & control , Brasil/epidemiología , Tuberculosis/diagnóstico , Tuberculosis/epidemiología , Incidencia , Prevalencia , Control de Infecciones , Humanos , Poblaciones VulnerablesRESUMEN
BACKGROUND: The application of automated analyses in neuroscience has become a practical approach. With automation, the algorithms and tools employed perform fast and accurate data analysis. It minimizes the inherent errors of manual analysis performed by a human experimenter. It also reduces the time required to analyze a large amount of data and the need for human and financial resources. METHODS: In this work, we describe a protocol for the automated analysis of the Morris Water Maze (MWM) and the Open Field (OF) test using the OpenCV library in Python. This simple protocol tracks mice navigation with high accuracy. RESULTS: In the MWM, both automated and manual analysis revealed similar results regarding the time the mice stayed in the target quadrant (p = 0.109). In the OF test, both automated and manual analysis revealed similar results regarding the time the mice stayed in the center (p = 0.520) and in the border (p = 0.503) of the field. CONCLUSIONS: The automated analysis protocol has several advantages over manual analysis. It saves time, reduces human errors, can be customized, and provides more consistent information about animal behavior during tests. We conclude that the automated protocol described here is reliable and provides consistent behavioral analysis in mice. This automated protocol could lead to deeper insight into behavioral neuroscience.
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Algoritmos , Programas Informáticos , Humanos , Ratones , Animales , Conducta AnimalRESUMEN
1. The objectives of this study were to establish the use of the fluorophores Hoechst 33342 and propidium iodide for the evaluation of sperm plasma membrane integrity and to identify an adequate hypoosmotic solution for the evaluation of sperm membrane functionality in quails.2. Sperm samples were collected from the vas deferens of nine quails. After initial evaluation, the samples were subjected to a flash-frozen assay. Three treatments with the following proportions of fresh sperm and sperm subjected to flash freezing were prepared as follows: 100:0 (T100), 50:50 (T50), and 0:100 (T0). The hypoosmotic swelling test used distilled water (0 mOsm/l) and fructose solutions (50, 100, and 200 mOsm/l).3. Immediately after recovery, the samples showed 75.6 ± 5.0% motility with vigour of 3.7 ± 0.3 and 96.1 ± 0.5% of the sperm appeared normal. The membrane integrity test showed 62.2 ± 5.2% intact sperm at T100, 29.0 ± 4.1% at T50 and 0.1 ± 0.1% at T0. Moreover, a greater number of reactive sperm (74.7 ± 6.7%) were observed when incubated in distilled water (0 mOsm/l) in comparison to other solutions (P < 0.05).4. The association of fluorescent probes composed of Hoechst 33342 and propidium iodide provided an efficient assessment of the integrity of the plasmatic membrane of quail spermatozoa. However, the study identified that the hypoosmotic swelling test has little predictive value regarding sperm membrane functionality in this species.
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Coturnix , Codorniz , Masculino , Animales , Propidio , Semen , Pollos , Espermatozoides , Membrana Celular , Colorantes Fluorescentes , Agua , Motilidad EspermáticaRESUMEN
Antimicrobial air filtration techniques have recently been widely studied to enhance indoor air quality and mitigate hazardous airborne microorganisms. Here, CuNPs were incorporated into a commercial polyester fiber surface and Scanning Mobility Particle Sizer was used to measure the adherence between fibers and nanoparticles. An acid pretreatment previous CuNP incorporation was effective against the particle release and enhanced the adhesion between particle and fiber. CuNP was a mixture of Cu0 and Cu2O with a diameter size of 90 nm (SEM micrographs). The permeability of the filter was low, in order of 10-9 m2. The activity against pathogens was tested in loco in a real environment using a filtration prototype apparatus. It was observed that the presence of CuNP mitigated the fungi and bacteria growth not only on the surface but also reduced microbe concentrations after passing through the filter. These results show that CuNP can be used as an inhibitor of various microorganisms, making them a good alternative for indoor environments to control indoor air quality.
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Betacoronavirus , Infecciones por Coronavirus/epidemiología , Neumonía Viral/epidemiología , Tuberculosis/diagnóstico , Tuberculosis/epidemiología , Brasil/epidemiología , COVID-19 , Infecciones por Coronavirus/diagnóstico , Infecciones por Coronavirus/terapia , Humanos , Pandemias , Neumonía Viral/diagnóstico , Neumonía Viral/terapia , SARS-CoV-2 , Tuberculosis/terapiaRESUMEN
Candida tropicalis is an emerging fungal pathogen associated with high mortality. We aimed to compare adherence capability of C. tropicalis to polystyrene and epithelial cell lines (HeLa and Vero), and determine whether adherent blastoconidia is cell-type specific. Blastoconidia adhesion to epithelial cells and polystyrene were determined by crystal violet assay. The percentage of epithelial cells with adhered blastoconidia and the number of adhered blastoconidia per cell line were determined by light microscopy. The correlation between adhesion surfaces was assessed by Pearson's correlation coefficient. The adhesiveness of C. tropicalis to polystyrene was greater than that observed for ephitelial cells. High correlation values (r2 0.9999222, p 0.007941) were found for the adhesion capability between biotic and polystyrene surface for isolates 100.10 (obtained from blood) and 335.07 (obtained from tracheal secretion). The number of adherent blastoconidia per HeLa cell was greater in comparison to that observed for Vero cells (P<0.05). Further, high correlation (r2 1, p 0.0001) was found for the adhesion ability between HeLa cells and Vero cells. The results suggest a correlation of C. tropicalis adhesion capability among different surfaces, and that the adhesion to epithelial cells is specific to the cell type.
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Candida tropicalis/fisiología , Adhesión Celular/fisiología , Células Epiteliales/microbiología , Poliestirenos , Animales , Candida tropicalis/aislamiento & purificación , Candida tropicalis/patogenicidad , Candida tropicalis/ultraestructura , Chlorocebus aethiops , Células Epiteliales/ultraestructura , Células HeLa , Humanos , Microscopía Confocal , Poliestirenos/química , Propiedades de Superficie , Células VeroRESUMEN
Polymyxins are important therapeutic options for treating infections, mainly those caused by carbapenem-resistant Klebsiella pneumoniae. Specific chemical characteristics of polymyxins make it difficult to perform antimicrobial susceptibility testing, especially within the clinical laboratory. Here we aimed to evaluate the performance of three phenotypic methods: Rapid NP Polymyxin Test, ColiSpot test and the SuperPolymyxin medium. To accomplish this, 170 non-duplicate clinical K. pneumoniae isolates were analysed (123 colistin-resistant and 47 susceptible). The sensitivity and specificity obtained for Rapid Polymyxin NP Test, Colispot and SuperPolymyxin medium were, respectively, 90% and 94%, 74% and 100%, and 82% and 85%. Very major errors occurred more frequently in low-level colistin-resistant isolates (MICs 4 and 8 µg/mL). Rapid Polymyxin NP proved to be a method capable of identifying colistin-resistant strains in acceptable categorical agreement. However, major errors and very major errors of this method were considered unacceptable for colistin-resistance screening. Although the Colispot test is promising and easy to perform and interpret, the results did not reproduce well in the isolates tested. The colistin-containing selective medium (SuperPolymyxin) showed limitations, including quantification of mucoid colonies and poor stability. Nevertheless, Colispot and SuperPolymyxin medium methods did not present acceptable sensitivity, specificity and categorical agreement. It is essential to use analytical tools that faithfully reproduce bacterial resistance in vitro, especially in last-line drugs, such as polymyxins, when misinterpretation of a test can result in therapeutic ineffectiveness.
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BACKGROUND: Despite the global decline in the detection of leprosy cases, its incidence has remained unchanged in certain settings and requires the determination of the factors linked to its persistence. We examined the spatial and space-time distribution of leprosy and the influence of social vulnerability on the occurrence of the disease in an endemic area of Northeast Brazil. METHODS: We performed an ecological study of all leprosy cases reported by Sergipe state, Northeast Brazil from 2001 to 2015, to examine the association of the Social Vulnerability Index and the prevalence and persistence of leprosy among the State's municipalities. Socio-economic and leprosy surveillance information was collected from the Brazilian information systems, and a Bayesian empirical local model was used to identify fluctuations of the indicators. Spatial and space-time clusters were identified using scan spatial statistic tests and to measure the municipalities' relative risk of leprosy. RESULTS: Leprosy clusters and burden of disease had a strong statistical association with the municipalities' Social Vulnerability Index. Municipalities with a high social vulnerability had higher leprosy incidence, multibacillary leprosy and newly diagnosed cases with grade 2 disability than areas with low social vulnerability. CONCLUSION: Social vulnerability is strongly associated with leprosy transmission and maintenance of disease incidence. Leprosy control programmes should be targeted to the populations with high social vulnerability.
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Lepra/epidemiología , Adolescente , Adulto , Teorema de Bayes , Brasil/epidemiología , Humanos , Factores de Riesgo , Adulto JovenRESUMEN
Experimental power is a measure of the ability of an experiment to detect differences between treatment means. Researchers design experiments and then calculate the probability that differences are simply due to chance, the null hypothesis. The objective of the analyses reported here was to determine the appropriate number of samples to demonstrate significant differences of various magnitudes from broiler chicken blood constituents. Over 800 samples were taken for a study of the effects of sample storage time, serum vs. plasma, light intensity, and fed vs. fasted birds on blood cholesterol, triglycerides, uric acid, glucose, total protein (TP), albumin, globulin, alanine aminotransferase (ALT), aspartate aminotransferase, gammaGT, creatinine, alkaline phosphatase, Ca and P. Various transformations increased the QQ plot R2 values from 0.000 to 0.149 or 0.00 to 17.62%. Most of the QQ plot R2 values were at or above 0.90. The 1/x2 transformation of blood P data showed the biggest increase in QQ plot R2 (0.846 to 0.995). The different standard deviations and coefficients of variation (CVs) found for each variable resulted in widely different numbers of replicates needed to detect differences in 2 treatment means. The extremes were glucose with a CV of 6.9% and ALT with a CV of 39.7%. For glucose, 15 replicates are needed to find a 10% difference in 97% of experiments; for ALT, 15 replicates would detect a 50% difference 91% of the time. The use of parameters such as cholesterol, glucose, TP, albumin, and globulin showed low CVs, indicating they may be considered as stable parameters. The lower CVs make it possible to find differences with a smaller number of replicates used in studies. As reported, the phosphorus values did not have a normal distribution of the data, so a transformation of these data could be an alternative to better discuss the results found.
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Análisis Químico de la Sangre/veterinaria , Pollos/sangre , Animales , Análisis Químico de la Sangre/métodos , Ayuno , Luz , Masculino , Plasma/química , Tamaño de la Muestra , Suero/química , Factores de TiempoRESUMEN
INTRODUCTION: The high morbidity and mortality caused by chronic kidney disease, and consequently, the increase in the need for kidney transplants, makes the evaluation of the kidney donation process relevant to verifying the service's fragility with the goal of optimizing this process. OBJECTIVE: This study analyzed the process of kidney donation in the Northern Macroregional area of Paraná, Brazil. METHOD: This was a cross-sectional and retrospective evaluation study based on 586 reports of deaths provided by the Intra-Hospital Organ Donation and Transplant Tissue Commissions to the Organ Procurement Organization of the Northern Macroregional area of Paraná in Brazil between 2011 and 2015. Data were analyzed by descriptive statistics, prevalence ratio, and Poisson regression. RESULTS: The prevalence of kidney donation was higher among the deaths of individuals younger than 61 years of age (P = .018) due to traumatic brain injury and hemorrhagic cardiovascular accident (P < .001), assisted in private institutions (P = .037), and occurring in the second half of the year (P = .015). CONCLUSION: The process of kidney donation was mainly compromised due to the refusal of the family and was associated with clinical and organizational aspects.
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Trasplante de Riñón , Obtención de Tejidos y Órganos/organización & administración , Adulto , Brasil , Estudios Transversales , Femenino , Humanos , Trasplante de Riñón/psicología , Masculino , Persona de Mediana Edad , Estudios Retrospectivos , Recolección de Tejidos y Órganos/psicología , Obtención de Tejidos y Órganos/estadística & datos numéricosRESUMEN
In light of forensic evidence indicating duplication and/or manipulation of western blot images the Editor-in-Chief is retracting the article cited above.
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This is one of the first studies to evaluate the effect of biometric variables (total length and weight), diet, and abiotic matrices (sediment and water column) on the bioaccumulation of methylmercury in tissues (muscle, liver, and gills) of four fish (two carnivore-invertivores, Pimelodus fur and Pachyurus adspersus; one carnivore-piscivore, Oligosarcus hepsetus; and one omnivore, Pimelodella lateristriga) in the lower section of a river in southeastern Brazil. Samples of fish (n = 120), water (n = 5) and sediment (n = 5) were collected at five sites characterized by pollution with mercury due to the use of organomercury fungicides and stream bed gold mining, commonly carried out in that section of the river in the 1980s. The results show that biometric variables are strongly correlated with methylmercury levels in muscle (r = 0.61, p < 0.0005) of P. fur. As a rule, concentrations of total mercury and methylmercury did not vary considerably between the organs of the species of different food habits, because of the environmental conditions in the study area. Despite the low concentrations of mercury in sediments (<0.05 mg kg-1 wet. wt), this compartment is a representative source of this pollutant for the organisms investigated, due to the close contact these animals keep with it in view of the low water columns in that section of the river.
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Monitoreo del Ambiente , Peces/metabolismo , Cadena Alimentaria , Compuestos de Metilmercurio/metabolismo , Contaminantes Químicos del Agua/metabolismo , Animales , Brasil , Branquias/química , Oro , Mercurio/análisis , Minería , Ríos , Contaminantes Químicos del Agua/análisisAsunto(s)
Glutatión Transferasa/genética , Mieloma Múltiple , Polimorfismo de Nucleótido Simple , Trasplante de Células Madre , Talidomida/administración & dosificación , Factor A de Crecimiento Endotelial Vascular/genética , Receptor 2 de Factores de Crecimiento Endotelial Vascular/genética , Autoinjertos , Supervivencia sin Enfermedad , Femenino , Humanos , Masculino , Mieloma Múltiple/genética , Mieloma Múltiple/mortalidad , Mieloma Múltiple/terapia , Tasa de SupervivenciaAsunto(s)
Gutatión-S-Transferasa pi/genética , Linfoma de Células B Grandes Difuso/genética , Linfoma de Células B Grandes Difuso/mortalidad , Mutación Missense , Polimorfismo Genético , Sustitución de Aminoácidos , Anticuerpos Monoclonales de Origen Murino/administración & dosificación , Protocolos de Quimioterapia Combinada Antineoplásica/administración & dosificación , Biomarcadores de Tumor , Ciclofosfamida/administración & dosificación , Supervivencia sin Enfermedad , Doxorrubicina/administración & dosificación , Femenino , Humanos , Linfoma de Células B Grandes Difuso/tratamiento farmacológico , Masculino , Prednisona/administración & dosificación , Estudios Prospectivos , Rituximab , Tasa de Supervivencia , Investigación Biomédica Traslacional , Vincristina/administración & dosificaciónRESUMEN
This study aimed to compare the effects of three different resistance exercise models on the quadriceps muscle cross-sectional area, as well as on mTOR phosphorylation and other pivotal molecules involved in the upstream regulation of mTOR. Twenty-four male Wistar rats were divided into untrained (control), endurance resistance training, strength resistance training, and hypertrophy resistance training (HRT) groups (n=6). After 12 weeks of training, the red portion of the quadriceps was removed for histological and Western blot analyses. The results showed that the quadriceps weight and cross-sectional areas in the exercised groups were higher than those of the untrained rats. However, the HRT group presented better results than the other two experimental groups. This same pattern was observed for mTOR phosphorylation and for the most pivotal molecules involved in the upstream control of mTOR (increase of PKB, 14-3-3, ERK, p38 MAPK, and 4E-BP1 phosphorylation, and reduction of tuberin, sestrin 2, REDD1, and phospho AMPK). In summary, our study showed that HRT leads to high levels of mTOR phosphorylation as well as of other proteins involved in the upstream regulation of mTOR.
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Fuerza Muscular/fisiología , Músculo Esquelético/anatomía & histología , Músculo Esquelético/fisiología , Condicionamiento Físico Animal/métodos , Proteínas Proto-Oncogénicas c-akt/metabolismo , Entrenamiento de Fuerza/métodos , Serina-Treonina Quinasas TOR/metabolismo , Animales , Masculino , Tamaño de los Órganos/fisiología , Ratas , Ratas Wistar , Resultado del TratamientoRESUMEN
BACKGROUND AND OBJECTIVE: Interleukin-6 (IL-6) is a powerful stimulator of osteoclast differentiation and bone resorption. Production of IL-6 is modulated by polymorphisms, and higher levels of this cytokine are found locally in patients with chronic periodontitis. In this study we performed a modern approach - Complete physical mapping of the IL6 gene - to identify the polymorphisms associated with chronic periodontitis in a southern Brazilian population sample. MATERIAL AND METHODS: One-hundred and nine individuals of both genders (mean age: 41.5 ± 8.5 years) were divided into a study group (56 participants with periodontitis) and a control group (53 individuals without periodontitis). After collection and purification of DNA, nine tag single nucleotide polymorphisms (SNPs; rs1524107, rs2069835, rs2069837, rs2069838, rs2069840, rs2069842, rs2069843, rs2069845 and rs2069849) covering the entire gene were selected according to the information available on the International HapMap Project website and evaluated using real-time PCR. RESULTS: Differences in the distribution of the following parameters were statistically significant between study and control groups: number of teeth (p = 0.030); probing depth (p < 0.001); clinical attachment level (p < 0.001); gingival index (p < 0.001); plaque index (p = 0.003); calculus index (p < 0.001); and dental mobility (p < 0.001). It was found that marker rs2069837 (located in intron 2 of IL6) under G dominant was associated with protection against chronic periodontitis in a Brazilian population in the presence of clinical variables, such as visible plaque, dentist visit frequency and dental floss use, and was suggested for the first time as a marker of susceptibility to chronic periodontitis. CONCLUSION: Complete physical mapping of IL6 (using tag SNPs) was carried out for the first time, unveiling allele G of polymorphism rs2069837 (located in the second intron of IL6) as a suggestive marker of protection against chronic periodontitis in a Brazilian population.
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Periodontitis Crónica/genética , Interleucina-6/genética , Adulto , Brasil , Estudios de Casos y Controles , Índice de Placa Dental , Femenino , Marcadores Genéticos/genética , Predisposición Genética a la Enfermedad/genética , Humanos , Interleucina-6/fisiología , Masculino , Índice Periodontal , Polimorfismo de Nucleótido Simple/genética , Reacción en Cadena en Tiempo Real de la PolimerasaRESUMEN
BACKGROUND: Urine monitoring programs represent an important strategy for early diagnosis of reactivation of BK polyomavirus (BKV) in kidney transplant recipients. This study analyzes a BKV urine screening model in kidney transplant patients. METHODS: Urinary screening for BKV reactivation was performed by urinary decoy cell and polymerase chain reaction (PCR) tests in samples from 32 consecutive kidney transplant patients, collected in a 6-month follow-up period. PCR in plasma samples and BKV immunohistochemical studies to assess BKV renal disease, if a kidney biopsy was indicated, were performed. RESULTS: The urinary screening for BKV among 32 renal receptors was positive in 18 patients (56%) by the concomitant use of the decoy cells and/or qualitative PCR at some time during the study period. Transfusion before transplantation was significantly associated with urinary decoy cell positive screening (odds ratio = 11; 95% confidence interval = 1.47 to 82.4; P < .05); and so was male sex (odds ratio = 2.02; 95% confidence interval = 1.07 to 3.83; P < .05). The clinical management of screening positive cases consisted of decreasing or changing the immunosuppression regimen. Sixteen renal biopsies were performed. Immunohistochemistry for SV40 T antigen was negative in all biopsies. After 1 year of follow-up, no patient developed BKV-associated nephropathy, and there was no difference in renal function between patients positive and negative for BKV urinary screening. CONCLUSIONS: Early urinary monitoring is effective in detection of BKV replication and represents a good strategy to minimize the deleterious effects caused by the presence of the virus on preservation of graft function.
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ADN Viral/orina , Enfermedades Renales/orina , Trasplante de Riñón , Infecciones por Polyomavirus/orina , Infecciones Tumorales por Virus/orina , Adulto , Virus BK/genética , Biopsia , Femenino , Rechazo de Injerto/prevención & control , Humanos , Inmunohistoquímica , Inmunosupresores/efectos adversos , Enfermedades Renales/diagnóstico , Enfermedades Renales/etiología , Enfermedades Renales/patología , Masculino , Tamizaje Masivo , Persona de Mediana Edad , Oportunidad Relativa , Proyectos Piloto , Reacción en Cadena de la Polimerasa , Infecciones por Polyomavirus/diagnóstico , Infecciones por Polyomavirus/etiología , Factores Sexuales , Receptores de Trasplantes , Infecciones Tumorales por Virus/diagnóstico , Infecciones Tumorales por Virus/etiología , UrinálisisRESUMEN
This study evaluated the levels of As, Cd, and Pb in muscle and liver the cattle and chicken. The risk was estimated for the adult population of a midsized city in southeast Brazil, concerning the tolerable ingestion and cancer risk. Samples of muscle and liver (cattle and chicken) were collected (n = 250). Samples of mineral supplements for cattle (n = 4) and chicken feed samples (n = 4) were evaluated as one of many potential source of contamination. Muscle, liver, mineral supplement, and feed samples were dissolved in acid medium and analyzed by ICP-OES. Daily muscle and liver intake was estimated using a questionnaire (N = 427). Daily intake of trace elements by the population based on the consumption of cattle muscle, cattle liver, chicken muscle, and chicken liver was low, corresponding to 2.76%, 0.33%, 2.12%, and 0.22% of the Tolerable Intake defined by the WHO for As; 0.54%, 0.29% 0.55%, 0.01%, for Cd; and 0.80%, 0.07%, 0.62%, 0.02%, for Pb. The mean of total ingestion of As, Cd and Pb was 5.43%, 1.18% and 1.51%, respectively of Tolerable Intake defined by WHO. Cancer risk was lower than 5 × 10-5 year-1. The results indicate that the muscle and liver consumption is a source of As, Cd, and Pb. Consumers that ingest cattle and chicken muscle need attention in terms the risk of cancer related to intake of As and Cd. Feed and mineral supplementation remain as one of many sources of exposure of As, Cd, and Pb.
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Arsénico/farmacocinética , Cadmio/farmacocinética , Contaminación de Alimentos/análisis , Plomo/farmacocinética , Hígado/metabolismo , Músculos/metabolismo , Neoplasias/epidemiología , Adulto , Animales , Arsénico/análisis , Arsénico/toxicidad , Intoxicación por Arsénico/epidemiología , Intoxicación por Arsénico/etiología , Brasil/epidemiología , Cadmio/análisis , Cadmio/toxicidad , Intoxicación por Cadmio/epidemiología , Intoxicación por Cadmio/etiología , Bovinos , Pollos , Femenino , Humanos , Plomo/análisis , Plomo/toxicidad , Intoxicación por Plomo/epidemiología , Intoxicación por Plomo/etiología , Masculino , Neoplasias/etiología , Medición de Riesgo , Distribución TisularRESUMEN
Sarcophagidae (Diptera) are potential vectors of several pathogens. They are also very important in forensic entomology, providing basic information on the circumstances of death. The objective of this study was to determine the synanthropic index of adult Sarcophagidae collected in Rio Claro, state of São Paulo, southeastern Brazil. Sampling occurred between September 2009 and August 2010. Traps baited with sardines, beef liver, or minced meat were set for five consecutive days per month in three distinct ecological areas representing urban, rural, and forest environments. A total of 440 specimens of sarcophagids were collected. The most abundant species was Peckia (Sarcodexia) lambens (Wiedemann), followed by Oxysarcodexia thornax (Walker), Peckia (Euboettcheria) collusor (Curran & Walley), Peckia (Euboettcheria) sp., and Peckia (Pattonella) intermutans (Walker). The only species with positive synanthropic index values were O. thornax and P. (S.) lambens, which demonstrated a greater preference for inhabited areas. Peckia (Euboettcheria) florencioi (Prado & Fonseca), P. (P.) intermutans, and Peckia (Euboettcheria) australis (Townsend) were only found in the forested area, which demonstrates their importance in forensic entomology because of their preference for a particular type of environment. The greatest number of sarcophagids was found in the forest environment; however, only the forest and rural areas were significantly different in Sarcophagidae abundance.