RESUMEN
BACKGROUND: The swine is a valuable model for preclinical research and surgical technique training. Induction of Type I diabetes is achieved by total pancreatectomy, therefore these animals may be used in several research studies, including islet transplantation field. Given the lack of information in the literature, the purpose of this work is to describe anatomic aspects of swine pancreas, the total pancreatectomy surgical technique, intra- and postoperative complications and the autopsy results. MATERIAL AND METHODS: Five hybrid male pigs, 20-35 kg, submitted to total pancreatectomy with duodenum, bile duct, and spleen preservation. Postoperatively, daily clinical assessment and capillary blood glucose collection were performed. At the end of the 30-day period or in the occurrence of serious clinical complications, euthanasia and autopsy were performed. RESULTS: The average duration of surgery was 128 minutes, without intraoperative deaths or anesthesia induction failures. The median survival was 6.6 days. Postoperative complications were weight loss (3), emesis (2), constipation (2), abdominal distension (2), diarrhea (1), and loss of appetite (1). All animals were euthanized due to serious complications. Two animals presented surgical complications (duodenal necrosis with gastroparesis and internal hernia with intestinal necrosis). The other 3 animals presented serious clinical complications related to exocrine pancreatic insufficiency due to deficiency of pancreatic enzymes. Glycemic values above 200 mg/dL were found on the first postoperative day and above 300 mg/dL on the seventh day in all animals. CONCLUSION: A model of total pancreatectomy with duodenum, spleen, and bile duct preservation in pigs was established. All animals became diabetic, however, animals without postoperative complications were euthanized due to serious complications related to pancreas exocrine insufficiency.
Asunto(s)
Páncreas , Pancreatectomía , Complicaciones Posoperatorias , Animales , Porcinos , Masculino , Complicaciones Posoperatorias/etiología , Páncreas/cirugía , Glucemia/análisis , Glucemia/metabolismoRESUMEN
Parasite communities correspond to the definition of metacommunity, as species interact and disperse within hosts. The present study evaluated parasite metacommunities in a tropical floodplain. The study was conducted in the Western Amazon around the municipalities of Cruzeiro do Sul, state of Acre, and Guajará, state of Amazonas, Brazil. Six sampling sites were selected and grouped into conserved and degraded environments. Fish were caught between periods of drought and flood, using passive and active sampling methods; in the laboratory, they were measured weighed, and necropsied. Parasites found were fixed, evaluated, and identified. Physical and chemical variables and environmental conservation characteristics were measured in all sites. Diversity index, ANOVA, Tukey, local contribution to beta diversity (LCBD), species contribution to beta diversity by individual species (SCBD), and variance partitioning were summarized. The α species diversity increased in conserved environments and varied between seasonal periods, mainly in detritivorous and omnivorous hosts. Local contributions to beta diversity showed significantly higher values in conserved environments for the endoparasite fauna of piscivorous and omnivorous hosts, indicating that these environments presented unique parasite infracommunities and revealing the conservation status of these environments. Variations in infracommunities were explained mainly by niche-based processes, including environmental conditions, degree of conservation, and host characteristics. Thus, these data will serve as a tool to understand the way parasite communities are structured, which is important information for the management and conservation of aquatic environments.