RESUMEN
In intensive swine production systems,, the practice of regrouping unfamiliar pigs is common, often leading to aggressive behavior. Although the effect of different musical genres composed for humans has been evaluated in pigs to mitigate aggression, there have been few attempts to create music specifically for pigs. Here, we assess whether sensory stimulation through music, created by adapting the acoustic parameters in the sound mix, induces changes in the aggressive behaviors of pigs during regrouping. Six litters of 10-week-old piglets were randomly selected and assigned to different treatments. The control group (Group A) received no intervention, while Group B was exposed to music for two continuous hours in the morning and afternoon from the time of regrouping. Group C received musical stimulation for one continuous hour in the morning following regrouping. A significant reduction in the frequency and duration of aggressive behaviors was observed in the groups that received musical stimulation during regrouping. Additionally, social, and individual play behaviors showed a decrease in the musical stimulation groups. These findings provide evidence for the effectiveness of created music as a strategy in reducing aggressive behavior during pig regrouping, which can enhance the welfare of pigs and offer a practical solution for pig producers to minimize aggression and its associated negative impacts.
RESUMEN
Music is a complex stimulus, with various spectro-temporal acoustic elements determining one of the most important attributes of music, the ability to elicit emotions. Effects of various musical acoustic elements on emotions in non-human animals have not been studied with an integrated approach. However, this knowledge is important to design music to provide environmental enrichment for non-human species. Thirty-nine instrumental musical pieces were composed and used to determine effects of various acoustic parameters on emotional responses in farm pigs. Video recordings (n = 50) of pigs in the nursery phase (7-9 week old) were gathered and emotional responses induced by stimuli were evaluated with Qualitative Behavioral Assessment (QBA). Non-parametric statistical models (Generalized Additive Models, Decision Trees, Random Forests, and XGBoost) were applied and compared to evaluate relationships between acoustic parameters and pigs' observed emotional responses. We concluded that musical structure affected emotional responses of pigs. The valence of modulated emotions depended on integrated and simultaneous interactions of various spectral and temporal structural components of music that can be readily modified. This new knowledge supports design of musical stimuli to be used as environmental enrichment for non-human animals.
Asunto(s)
Música , Animales , Porcinos , Música/psicología , Estimulación Acústica , Emociones/fisiología , Acústica , Percepción Auditiva/fisiologíaRESUMEN
Plant natural products can attenuate the myonecrosis caused by Bothrops snake venom and their phospholipases A2 (PLA2). In this study, we evaluated the effects of two fractions (F4 and F6) from Swietenia macrophylla and purified catechin on the muscle damage caused by a myotoxic PLA2 from Colombian Bothrops asper venom (BaColPLA2) in mice and by Bothrops marmoratus venom from Brazil in mouse phrenic nerve-diaphragm muscle (PND) preparations in vitro. Male mice were injected with PLA2 (50 µg) in the absence or presence of F4, F6, and catechin, in the gastrocnemius muscle and then killed 3, 7, 14, and 28 h later for histopathological analysis of myonecrosis, leukocyte infiltration, and the presence of collagen. Fractions F4 and F6 (500 µg) and catechin (90 µg) significantly reduced the extent of necrosis at all-time intervals. These two fractions and catechin also attenuated the leukocyte infiltration on day 3, as did catechin on day 14. There was medium-to-moderate collagen deposition in all groups up to day 7, but greater deposition on days 14 and 28 in the presence of F6 and catechin. Bothrops marmoratus venom (100 µg/mL) caused slight (~25%) muscle facilitation after 10 minutes and weak neuromuscular blockade (~64% decrease in contractile activity after a 120-minute incubation). Pre-incubation of venom with F4 or F6 abolished the facilitation, whereas catechin, which was itself facilitatory, did not. All three fractions attenuated the venom-induced decrease in muscle contractions. These findings indicate that fractions and catechin from S. macrophylla can reduce the muscle damage caused by Bothrops venom and PLA2. These fractions or their components could be useful for treating venom-induced local damage.
Asunto(s)
Catequina/uso terapéutico , Venenos de Crotálidos/toxicidad , Meliaceae , Músculo Esquelético/efectos de los fármacos , Fosfolipasas A2/toxicidad , Nervio Frénico/efectos de los fármacos , Extractos Vegetales/uso terapéutico , Animales , Bothrops , Fibrosis/inducido químicamente , Fibrosis/tratamiento farmacológico , Masculino , Ratones Endogámicos BALB C , Contracción Muscular/efectos de los fármacos , Músculo Esquelético/patología , Músculo Esquelético/fisiología , Necrosis/inducido químicamente , Necrosis/tratamiento farmacológico , Nervio Frénico/fisiologíaRESUMEN
BACKGROUND: Normal mammary gland contains an extravascular population of B lymphoblasts, precursors of the immunoglobulin plasma cells that play a key role in the passive protection of neonates by secreting immunoglobulins to colostrum and milk. We investigated the presence of chemoattractants in the milk by analysing the chemoattractant activity of various fractions of this secretion. Milk chemoattractants are potentially involved in the recruitment of lymphocytes from the maternal bloodstream in lactating mammary glands. RESULTS: The dilution-related lymphoid cell chemoattraction of whey was associated with a < 10 kDa ultrafiltrate. Active fractions were purified by reverse-phase high performance liquid chromatography. Two peptides of 2.7 kDa (DMREANYKNSDKYFHARGNYDAA) and 1 kDa (RPPGLPDKY) were identified as fragments of the SAA protein family, tentatively identified as SAA2. Only the 2.7 kDa synthetic peptide displayed chemotactic activity, at two different optimal concentrations. At the lower concentration (3.7 nM), it attracted B-cell lymphoblasts, whereas at the higher (3.7 microM), it attracted B lymphocytes. Then, the SAA mRNA expression was analysed and we observed more SAA transcripts during lactation than gestation. CONCLUSION: These data are consistent with the SAA23-45 fragment being involved in preplasma B-cell recruitment to the mammary gland and resultant benefit to the neonate.