RESUMEN
The aim of this study was to evaluate the influence of agitation techniques on bacterial reduction in curved root canals. Eighty human mandibular molars were prepared, inoculated with Enterococcus faecalis and incubated at 37°C for 60 days. Then, specimens were randomly separated into two test groups (n = 36) and two control groups (n = 04) according to agitation technique: Passive ultrasonic irrigation (PUI, Irrisonic) and XP-endo Finisher (XPF). Microbial samples were collected before and after instrumentation and after final agitation using sterile paper points. Bacterial growth was analysed by turbidity of culture medium and UV spectrophotometry. The Wilcoxon rank test was used for the paired analysis, while the Mann-Whitney U-test was used for the non-paired analysis. The samples collected after final agitation were significantly different between test groups (p < 0.05). Bacterial reduction was greater in the PUI than in the XPF (p < 0.05) group. The irrigant agitation provided significant bacterial reduction. The use of the PUI showed better results.
Asunto(s)
Cavidad Pulpar , Preparación del Conducto Radicular , Humanos , Cavidad Pulpar/microbiología , Enterococcus faecalis , Diente Molar , Irrigantes del Conducto Radicular/uso terapéutico , Preparación del Conducto Radicular/métodos , Hipoclorito de Sodio , Irrigación Terapéutica/métodos , UltrasonidoRESUMEN
The objective of this study was to evaluate the antibacterial effect and the ultrastructural alterations of diode laser with different wavelengths (808nm and 970nm) and its association with irrigating solutions (2.5% sodium hypochlorite and 2% chlorhexidine) in root dentin contaminated by a five days biofilm. Thirteen uniradicular teeth were sectioned into 100 dentin intraradicular blocks. Initially, the blocks were immersed for 5min in 17% EDTA and washed with distilled water for 5min, then samples were sterilized for 30min at 120°C. The dentin samples were inoculated with 0.1mL of E. faecalis suspension in 5mL BHI (Brain Heart Infusion) and incubated at 37°C for 5days. After contamination, the specimens were distributed into ten groups (n=10) according to surface treatment: GI - 5mL NaOCl 2.5%, GII - 5mL NaOCl 2.5%+808nm diode (0.1W for 20s), GIII - 5mL NaOCl 2.5%+970nm diode (0.5W for 4s), GIV - 808nm diode (0.1W for 20s), GV - 970nm diode (0.5W for 4s), GVI - CHX 2%, GVII - CHX 2%+808nm diode (0.1W for 20s), GVIII - CHX 2%+970nm diode (0.5W for 4s), GIX - positive control and GX - negative control. Bacterial growth was analyzed by turbidity and optical density of the growth medium by spectrophotometry (nm). Then, the specimens were processed for analysis ultrastructural changes of the dentin surface by SEM. The data was subject to the One-way ANOVA test. GI (77.5±12.1), GII (72.5±12.2), GIII (68.7±8.7), GV (68.3±8.7), GVI (62.0±5.5) and GVII (67.5±3.3) were statistically similar and statistically different from GIV (58.8±25.0), GVIII (59.2±4.0) and control groups (p<0.05). SEM analysis showed a modified amorphous organic matrix layer with melted intertubular dentin when dentin samples were irradiated with 970nm diode laser; erosion of the intertubular dentin in blocks submitted to 808nm diode laser irradiation; and an increased erosion of the intertubular dentin when 2.5% NaOCl was associated to the different wavelengths lasers. All the therapeutic protocols were able to reduce the bacterial contingent in dentin blocks, and the association of diode laser and solutions did not significantly improve the reduction of the bacterial contingent.
Asunto(s)
Enterococcus faecalis/efectos de la radiación , Láseres de Semiconductores , Biopelículas/efectos de los fármacos , Biopelículas/efectos de la radiación , Clorhexidina/farmacología , Dentina/microbiología , Dentina/ultraestructura , Ácido Edético/farmacología , Enterococcus faecalis/efectos de los fármacos , Enterococcus faecalis/fisiología , Humanos , Microscopía Electrónica de Rastreo , Hipoclorito de Sodio/farmacología , EspectrofotometríaRESUMEN
INTRODUCTION: Autogenous transplantation of a natural tooth to another site has significant advantages over dental implants, particularly in cases of agenesis, accidental tooth loss, or poor prognosis for the maintenance of tooth function. METHODS: This report describes a case of autogenous premolar transplantation into an artificial socket in the site of a missing maxillary lateral incisor in a 13-year-old girl. Clinical examination and radiography revealed tooth agenesis (#4, #10, #13, and #20) and microdontia (#7). The occlusion and skeletal maxillomandibular relations were normal. RESULTS: Tooth #29 was chosen for transplantation into the site of tooth #10 because of its size, stage of root formation, and possible closure of the spaces created by agenesis. CONCLUSIONS: Autogenous transplantation is a feasible alternative to dental implants in cases of tooth agenesis or tooth loss because of trauma. Autotransplantation was indicated in this case because it ensures the natural (facial) growth of the alveolar process and preserves the function of periodontal tissues. A multidisciplinary approach (ie, combining techniques from different dental specialties) was important for treatment success. Clinical and radiographic follow-up confirmed that the transplanted premolar was esthetically comparable with the lateral incisor and that root development and pulp canal obliteration were complete.
Asunto(s)
Autoinjertos/trasplante , Diente Premolar/trasplante , Incisivo , Maxilar/cirugía , Alveolo Dental/cirugía , Adolescente , Anodoncia/terapia , Diente Premolar/crecimiento & desarrollo , Pulpa Dental/diagnóstico por imagen , Estética Dental , Femenino , Estudios de Seguimiento , Humanos , Incisivo/anomalías , Planificación de Atención al Paciente , Radiografía , Técnicas de Movimiento Dental/métodos , Raíz del Diente/crecimiento & desarrollo , Resultado del TratamientoRESUMEN
INTRODUCTION: The aim of this study was to evaluate a method to measure inflammatory root resorption (IRR) by using cone beam computed tomography (CBCT) scans. METHODS: IRR sites were classified according to root third and root surface, and IRR extension was measured on the axial, transverse, and tangent views of 3-dimensional CBCT scans by using the Planimp software. A 5-point (0-4) scoring system was used to measure the largest extension of root resorption. A total of 48 periapical radiographs and CBCT scans originally taken from 40 patients were evaluated. The kappa coefficient was used to assess interobserver agreement and the chi(2) test to determine significant differences between the imaging methods. The level of significance was set at alpha = 1%. RESULTS: IRR was detected in 68.8% (83 root surfaces) of the radiographs and 100% (154 root surfaces) of the CBCT scans (P < .001). The extension of IRR was >1-4 mm in 95.8% of the CBCT images and in 52.1% of the images obtained by using the conventional method (P < .001). CONCLUSIONS: CBCT seems to be useful in the evaluation of IRR, and its diagnostic performance was better than that of periapical radiography.