RESUMEN
The present study investigated the effects of aquatic exercise on parameters of functional autonomy, mental health, and oxidative dysfunction in elderly patients with DM2. A total of 130 elderly were included in the longitudinal clinical study and were attributed to the non-diabetic group (n = 27) and diabetes the group (n = 22). Both groups participated in 24 sessions of Hydro-HIIT, 48 h before and after Hydro-HIIT, the GDLAM index, depression, and anxiety scores and markers of oxidative dysfunction were quantified. After intervention, GI decreased in both groups (non-diabetes group = -24%; diabetes group = -22%) (p < 0.05), markers of depression (-46%), anxiety (-60%), DCFH-DA (-55%), SOD (+59%), TNF-α (-37%) and IL-1 (-48%) in diabetes group (p < 0.05). The intervention with Hydro-HIIT improves aspects related to functional autonomy, mental health, and exerts consequently, a modulating effect on oxidative stress and inflammatory response in elderly people diagnosed with DM2.
Asunto(s)
Diabetes Mellitus Tipo 2 , Entrenamiento de Intervalos de Alta Intensidad , Humanos , Anciano , Diabetes Mellitus Tipo 2/terapia , Salud Mental , Ejercicio Físico , Estrés OxidativoRESUMEN
Reactive oxygen species (ROS) are important in the pathogenesis of pulmonary injury induced by cigarette smoke (CS) exposure, and physical exercise (Ex) is useful in combating impaired oxidative process. We verified the preventive effects of Ex on lung oxidative markers induced by smoking. In this study, 36 mice (C57BL-6, 30-35 g) were split into four groups: control, CS, Ex, and CS plus Ex. Ex groups were given prior physical training in water (2×30 min/d, 5 days/wk, 8 weeks). After training, the CS groups were subjected to passive exposure to four cigarettes, 3 × per day, for 60 consecutive days. After 24 hours from the last exposure, CS animals were sacrificed, and lung samples were collected for further analysis. Left lung sample was prepared for histological analysis, and right lung was used for biochemical analysis (superoxide, hydroxyproline, lipid peroxidation [thiobarbituric acid reactive species], protein carbonylation [carbonyl groups formation], superoxide dismutase [SOD], catalase [CAT], and glutathione peroxidase [GPx] activities). Group comparisons were evaluated by analysis of variance (ANOVA). Results were expressed as mean ± standard deviation, with P<0.05 considered significantly different. Preventive Ex impeded histological changes and increased the enzymatic defense system (SOD and GPx) by reducing oxidative damage in lipids and proteins. This preventive effect of prior physical Ex alleviates damage caused by CS exposure.
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Pulmón/efectos de los fármacos , Pulmón/metabolismo , Estrés Oxidativo/efectos de los fármacos , Condicionamiento Físico Animal , Contaminación por Humo de Tabaco/efectos adversos , Animales , Masculino , Ratones , Ratones Endogámicos C57BLRESUMEN
It has been identified worldwide that amphibians are experiencing massive population declines. This decrease could be further enhanced by the exposure of amphibians to pollutants, which would enhance reactive oxygen species production and cause subsequent alterations in oxidant defense levels. The present study was aimed at understanding the impact of mineral coal on amphibians. For this purpose, chemical elemental contents and oxidative stress indexes in Hypsiboas faber from coal-mining areas and in an unpolluted area in the Catarinense Coal Basin, Brazil, were assessed. The highest contents of sulfur, chlorine, iron, zinc, and bromine were registered in specimens from the coal-mining area, whereas the highest contents of potassium calcium, and silicon were registered in specimens from the control area. It was found that there was a significant increase (p < 0.05) in the activity of super oxide dismutase (SOD) and glutathione peroxidase (GPx) in the animals from the coal-mining area, whereas the level of catalase showed no differences between the animal groups. The levels of TBARS showed no differences between the tested groups. However, carbonylation decreased significantly (p < 0.05) in animals from the coal-mining area, and there was a significant increase (p < 0.05) in the formation of total thiols in animals from the coal-mining area. In conclusion, the antioxidant system of H. faber is sensitive to pollutants present in coal-mining wastes, and its SOD and GPx activity may be a potential biomarker for monitoring the level of contaminants in the environment.
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Metales Pesados/metabolismo , Contaminantes Químicos del Agua/metabolismo , Animales , Anuros/metabolismo , Biomarcadores/metabolismo , Brasil , Catalasa/metabolismo , Minas de Carbón , Glutatión Peroxidasa/metabolismo , Metales Pesados/toxicidad , Estrés Oxidativo , Especies Reactivas de Oxígeno/metabolismo , Superóxido Dismutasa/metabolismo , Sustancias Reactivas al Ácido Tiobarbitúrico/metabolismo , Contaminantes Químicos del Agua/toxicidadRESUMEN
INTRODUCTION: The aim of the study was to evaluate the effects of TPU together with DMSO on oxidative stress parameters after eccentric exercise. METHODS: Thirty and six animals were divided in control; eccentric exercise (EE); EE+saline gel 0.9%; EE+TPU 0.8 W/cm(2); EE+DMSO gel; EE+TPU+DMSO gel and submitted to one 90-min downhill run (1.0 km h(-1)). TPU was used 2, 12, 24, 46 h after exercise session and 48 h after the animals were killed and the gastrocnemius muscles were surgically removed. Production of superoxide anion, creatine kinase (CK) levels, lipoperoxidation, carbonylation, and antioxidants enzymes were analyzed. RESULTS: Showed that TPU and gel-DMSO improved muscle healing. Moreover, superoxide anion production, TBARS level and protein carbonyls levels, superoxide dismutase and catalase activity were all decreased in the group TPU plus gel-DMSO. DISCUSSION: Our results show that DMSO is effective in the reduction of the muscular lesion and in the oxidative stress after eccentric exercise only when used with TPU.