RESUMEN
Radiation therapy is commonly applied in breast cancer (BC) patients. However, radioresistance and side effects are limiting factors of this practice. Therefore, studying substances that can enhance the radiation effect and, at the same time, protect normal cells is very relevant. Thus, the aim of this work was to assess the radiosensitizer effect of resveratrol (RV) on BC cells (MCF-7). A high cytotoxic and antiproliferative effect was observed in the treatment with 10 µM of RV + 3 Gy ionizing radiation (IR). Our results indicate that, 24 h after the exposition of cell cultures to RV + IR, an induction of necrosis/senescence has occurred. Furthermore, was observed the activation of extrinsic apoptosis pathway through a decrease of the Bax/Bcl-2 ratio and a high activity of caspase 8. Moreover, our data show that this treatment affected the oxidative cell metabolism, increasing oxidative protein, lipid and membrane damage and also acted to decrease the antioxidant enzymes activity. The antiproliferative effect on 72 h cultures may be associated with a high expression of p53 and an interruption of cell cycle in the S phase. Therefore, our results suggest that RV is a potential radiosensitizer of MCF-7 BC cells.
Asunto(s)
Neoplasias de la Mama/tratamiento farmacológico , Fármacos Sensibilizantes a Radiaciones/farmacología , Estilbenos/farmacología , Antioxidantes/metabolismo , Apoptosis/efectos de los fármacos , Neoplasias de la Mama/metabolismo , Ciclo Celular/efectos de los fármacos , Línea Celular Tumoral , Proliferación Celular/efectos de los fármacos , Femenino , Humanos , Células MCF-7 , Resveratrol , Transducción de Señal/efectos de los fármacos , Proteína p53 Supresora de Tumor/metabolismoRESUMEN
Nowadays there is an increase in the number of people taking herbals worldwide. Scutia buxifolia is used for the treatment of hypertension, but little is known about its action on liver. Thirty-two Wistar rats were divided into four groups: control and groups treated during 30 days with 100, 200 and 400 mg of lyophilized aqueous extract of S. buxifolia (SBSB)/kg of body weight. This study was planned to explore hepatotoxic effect of SBSB, which was assessed by serum transaminases (ALT and AST). Thiobarbituric acid reactive substances (TBARS) levels were determined in liver, along with thiols content (NPSH), catalase (CAT) activity and, superoxide dismutase (SOD) enzymes. Histopathological studies of liver tissue were performed. Flavonoids and phenolics were quantified in SBSB by high performance liquid chromatography with diode array detection (HPLC/DAD). We did not observe alterations on redox status (TBARS, NPSH, CAT and, SOD) in the control and experimental groups. An increase on AST activity was only observed at 200 mg of SBSB, whereas ALT score was not affected by SBSB. Moreover, no morphological alterations were observed on the hepatocytes, matching the analysed biochemical parameters. This way, we conclude that SBSB was not toxic.
Asunto(s)
Hígado/efectos de los fármacos , Extractos Vegetales/toxicidad , Rhamnaceae/metabolismo , Animales , Antioxidantes/análisis , Catalasa/efectos de los fármacos , Catalasa/metabolismo , Flavonoides/análisis , Medicina de Hierbas , Pruebas de Función Hepática , Oxidación-Reducción/efectos de los fármacos , Ratas , Ratas Wistar , Superóxido Dismutasa/efectos de los fármacos , Superóxido Dismutasa/metabolismo , Sustancias Reactivas al Ácido Tiobarbitúrico/análisis , Transaminasas/efectos de los fármacosRESUMEN
Phytochemical analysis of lyophilised aqueous extract of the stem bark of Scutia buxifolia (SBSB) was carried out by determining total phenolics (0.280 ± 0.02 mg of gallic acid equivalents/g of extract), flavonoids (17.42 ± 2.95 mg of quercetin equivalents/g of extract) and tannins (1.28 ± 0.15 mg of catechin equivalents/g of extract) contents followed by a high-performance liquid chromatography/diode array detection (HPLC/DAD) analysis. The HPLC profile showed caffeic acid, being the major constituent of SBSB (247.21 ± 2.17 mg g⻹ of extract). The antioxidant scavenging capacity of SBSB was determined by 2,2-diphenyl-2-picrylhydrazyl (DPPH) assay. The antioxidant power of SBSB was comparable with that of the antioxidant ascorbic acid. Acute toxicity was assayed in rats whereas catalase activity and malondialdehyde production were determined in rats' liver. The SBSB showed safety in the dose tested. This report is the first realised in animals for S. buxifolia.