RESUMEN

Stromal cells from pediatric myelodysplastic syndromes (MDS) and acute myeloid leukemia (AML) associated with MDS (MDS-AML) present high expression of leukemia inhibitor factor (LIF). We demonstrated using mitogen-activated protein kinase (MAPK) inhibitors that in stromal cells from pediatric MDS and MDS-AML, p38MAPK was critical in serum-induced secretion of LIF. The serum induction of phosphorylated p38MAPK form was observed only in stromal cells from healthy children, whereas in MDS and MDS-AML basal levels were maintained suggesting constitutive p38MAPK activation. Our study suggested the possible importance in pediatric MDS of p38MAPK signaling pathway which may be a future therapeutic target.

Asunto(s)

Células de la Médula Ósea/metabolismo , Factor Inhibidor de Leucemia/metabolismo , Síndromes Mielodisplásicos/metabolismo , Células del Estroma/metabolismo , Proteínas Quinasas p38 Activadas por Mitógenos/fisiología , Antracenos/farmacología , Células de la Médula Ósea/patología , Células Cultivadas , Niño , Preescolar , Femenino , Flavonoides/farmacología , Humanos , Imidazoles/farmacología , Lactante , Masculino , Síndromes Mielodisplásicos/patología , Inhibidores de Proteínas Quinasas/farmacología , Piridinas/farmacología , Suero/fisiología , Células del Estroma/patología , Proteínas Quinasas p38 Activadas por Mitógenos/antagonistas & inhibidores