RESUMEN
An Ultra performance liquid chromatography (UPLC) coupled to UV detection method was developed to determine acetamiprid residues in water reservoirs of northern Benin, close to cotton fields. The quantification limit of this method was 0.2 µg L-1 acetamiprid in water, its precision ranged between 8% and 22%, and its trueness between 99% and 117% (for concentrations ranging from 0.2 to 5.0 µg L-1). Acetamiprid residues were determined in water samples collected in four reservoirs from northern Benin during the phytosanitary treatment period of cotton. The minimum and maximum concentrations of acetamiprid residues in water were 0.2 and 7.7 µg L-1, respectively. These levels do not represent any risk for human consumption of this water, but indicate a regular use of acetamiprid, possibly together with other pesticides which could be more harmful for both humans and aquatic species.