RESUMEN
In view of the current public health hazards of food-borne pathogens, it is urgent to develop a rapid detection method with high sensitivity, good specificity and operational convenience, as well as to determine an effective sterilization strategy. Herein, versatile gold-ruthenium nanocomposites modified with antibody (Au@Ru-pAb Ncs) have been constructed for the sensitive detection of Salmonella typhimurium (S. typhimurium) via the lateral flow immunochromatographic assay (LFIA). Au@Ru-pAb Ncs based LFIA exhibited a wide detection range from 2.9 × 106 CFU/mL to 2.9 × 1011 CFU/mL with the limit of detection of 9.8 × 104 CFU/mL for S. typhimurium, and displayed excellent specificity. In addition, Au@Ru-pAb Ncs irradiated with 808 nm (500 mW/cm2) near-infrared light (NIR) had a significant antibacterial effect within only 5 min, attributed to its high photothermal conversion efficiency of 54.14%. Therefore, both sensitive detection of S. typhimurium and effective NIR-triggered photothermal sterilization were achieved by using versatile Au@Ru-pAb Ncs, showing great prospects in the field of pathogen detection and treatment.
Asunto(s)
Nanocompuestos , Salmonella typhimurium , Oro/química , Rayos Infrarrojos , Nanocompuestos/química , EsterilizaciónRESUMEN
As a neglected member of the platinum group elements, osmium, the metal with the highest density in the earth, is very suitable for the preparation of a peroxidase with high catalytic activity and stability, and can also be associated with the development of a sensor. In this study, we accessed Os nano-hydrangeas (OsNHs) with one-pot synthesis and utilized them in a bifunctional immunosensor that can present both catalytic chromogenic and tinctorial signal for nanozyme-linked immunosorbent assay (NLISA) and lateral flow immunoassay (LFIA) for use in folic acid (FA) detection. In the OsNHs-NLISA, the linear range is from 9.42 to 167.53 ng mL-1. The limit of detection (LOD) is 4.03 ng mL-1 and the IC50 value is 39.73 ng mL-1. In OsNHs-LFIA, the visual cut-off value and limit of detection (v-LOD) are 100 ng mL-1 and 0.01 ng mL-1, respectively. Additionally, the outcome from the specificity and spiked sample analysis offered recovery from the spiked milk powder sample ranging from 93.9 to 103.6% with a coefficient of variation under 4.9%, compared with UPLC-MS/MS for a correlation of R2 = 0.999 and admirable validation. The promising application of the OsNHs can also be used in other bioprobes, and this bifunctional immunosensor analysis mode is suitable for diversified analytes.
Asunto(s)
Técnicas Biosensibles , Hydrangea , Cromatografía Liquida , Ácido Fólico , Inmunoensayo , Osmio , Espectrometría de Masas en TándemRESUMEN
Currently, the infection with Helicobacter pylori affects about half of the world's population, and the most common therapy to treat H. pylori is the first line clarithromycin-based triple therapy or the quadruple therapy. However, drug resistance, eradication in a low level, high rate of reinfection, and gastrointestinal side effects among the causative organisms for H. pylori infection pose a critical challenge to the global health care community. Therefore, new approaches to treat H. pylori infections are urgently needed. Chicken egg yolk constituting a source of immunoglobulin Y (IgY) has attracted noticeable attention for its advantages of cost-effective extraction, minimization of animal harm and suffering, and induction of no specific resistance and is, therefore, being regarded as an alternative therapy for H. pylori infection. This review is intended to summarize various H. pylori antigens for IgY preparation in terms of their application, mechanism, and limitations.
Asunto(s)
Helicobacter pylori , Animales , Anticuerpos , Yema de Huevo , Inmunoglobulinas , UreasaRESUMEN
In this paper, five fluorescein-labeled dehydroepiandrosterone (DHEA) derivatives (tracers) with different chain lengths between the fluorescein and hapten were synthesized and featured so as to establish a fluorescence polarization immunoassay (FPIA) for DHEA detection in human urine samples with previously prepared polyclonal antibody against DHEA. The outcomes of the structure of tracer on FPIA sensitivity were investigated. Under the optimal condition, the fluorescence polarization value (FP) decreases linearly in DHEA concentration, ranging from 1.6 to 243.3 ng mL-1, with the limit of detection of 1.1 ng mL-1 and IC50 value of 25.1 ng mL-1. Moreover, the developed FPIA was time-saving as it could complete the detection within 3 min. FPIA and commercial enzyme-linked immunosorbent assay kit were both applied to analyze the spiked human urine samples with DHEA. Excellent recoveries (92.1-108.0%) and satisfactory correlation coefficient (R2 = 0.98) were acquired with the two methods, indicating that the developed FPIA was a fast and efficient screening immunoassay with accuracy and sensitivity for DHEA detection in human urine samples. Graphical abstract.