RESUMEN
This work presents data on the relationship between the oligomeric state and catalytic activity of ß-like DNA polymerases isolated from three cancer cell lines upon replacement of magnesium ions with iron. It was shown that the suppression of the catalytic activity of ß-like DNA polymerases in the presence of divalent iron ions is related to enzyme oligomerization that can be responsible for the suppression of the magnetic isotope effect.
Asunto(s)
ADN Polimerasa Dirigida por ADN , Magnesio , Iones , Hierro , Isótopos , Magnesio/metabolismo , Magnesio/farmacologíaRESUMEN
Pituitary tumor-transforming gene-1 (PTTG1) encodes securin, a multifunctional protein involved in development of various types of cancer. Securin participates in the regulation of sister chromatids separation and the expression of multiple genes involved in the control of the cell cycle, metabolism, and angiogenesis. In several human cell lines, we have found a novel short isoform of securin mRNA, which does not contain exons 3 and 4. After the translation of this new mRNA, a shortened protein is produced that, like the full-size form, is able to activate the transcription of cyclin D3 gene (CCND3), which controls the G1/S transition and angiogenesis factors VEGFA (vascular endothelial growth factor), and FGF2 (fibroblast growth factor 2) in HEK293 cells. However, unlike the full-size protein, the short isoform of PTTG1 does not affect the MYC gene expression because it lacks the DNA-binding domain, which is needed for its interactions with the MYC promoter. Furthermore, the short form of securin does not influence the expression of MYC transcriptional targets, such as TP53 and IL-8. Thus, we found a novel isoform of securin which is able to activate a more restricted repertoire of genes compared to the full-size protein.
Asunto(s)
Ciclina D3/biosíntesis , Factor 2 de Crecimiento de Fibroblastos/biosíntesis , Regulación Neoplásica de la Expresión Génica , Proteínas Proto-Oncogénicas c-myc/biosíntesis , Securina/metabolismo , Factor A de Crecimiento Endotelial Vascular/biosíntesis , Ciclina D3/genética , Factor 2 de Crecimiento de Fibroblastos/genética , Células HEK293 , Células Hep G2 , Humanos , Células Jurkat , Células K562 , Células MCF-7 , Isoformas de Proteínas/biosíntesis , Isoformas de Proteínas/genética , Proteínas Proto-Oncogénicas c-myc/genética , Securina/genética , Factor A de Crecimiento Endotelial Vascular/genéticaRESUMEN
The molecular dynamics method has been applied to investigate the conformational behavior of biologically important chiral molecules of cholesterol and ergosterol. The formation of strings in the solution of cholesterol in methanol and the lack of strings in solutions of ergosterol in methanol has been experimentally detected. It was shown that the intermolecular dynamics in the molecule has a significant impact on the potential of structure formation. We proposed alternative explanation of the functional significance of cholesterol, apparently associated with the formation of interconnect structures outside the membrane as the biological feasibility of finding ergosterol in non-switched cells of fungi and cholesterol in the switching cells of macroorganisms.
Asunto(s)
Colesterol/química , Ergosterol/química , Membrana Dobles de Lípidos/química , Conformación Molecular , Geles/química , Simulación de Dinámica Molecular , Soluciones/químicaRESUMEN
The phycobilisome (PBS) is a major light-harvesting complex in cyanobacteria and red algae. To obtain the detailed structure of the hemidiscoidal PBS core composed of allophycocyanin (APC) and minor polypeptide components, we analyzed all nine available 3D structures of APCs from different photosynthetic species and found several variants of crystal packing that potentially correspond to PBS core organization. Combination of face-to-face APC trimer crystal packing with back-to-back APC hexamer packing suggests two variants of the tricylindrical PBS core. To choose one of these structures, a computational model of the PBS core complex and photosystem II (PSII) dimer with minimized distance between the terminal PBS emitters and neighboring antenna chlorophylls was built. In the selected model, the distance between two types of pigments does not exceed 37 Å corresponding to the Förster mechanism of energy transfer. We also propose a model of PBS and photosystem I (PSI) monomer interaction showing a possibility of supercomplex formation and direct energy transfer from the PBS to PSI.
Asunto(s)
Complejo de Proteína del Fotosistema I/química , Complejo de Proteína del Fotosistema II/química , Ficobilisomas/química , Clorofila/química , Cianobacterias/metabolismo , Estructura Molecular , Rhodophyta/metabolismoRESUMEN
We report on theoretical efficiency of non-photochemical fluorescense quenching of phycobilisomes by the orange carotenoid protein. The created 3D computer model of the three-cylindrical phycobilisomes core allowed us to determine the distances between centers of mass of all phycobilin chromophores of the core and calculate the time and an average number of energy migration steps for the resulting non-radiative excitation transfer from the phycobilisomes to photosystem II. The obtained kinetic scheme equations for a way of energy transfer confirm the incomplete interception of energy flow in the phycobilisomes core by the orange carotenoid protein. Theoretical estimation of the rate of phycobilisomes quenching is in good agreement with experimental data.
Asunto(s)
Simulación por Computador , Fluorescencia , Ficobilisomas/química , Proteínas Bacterianas/química , Proteínas Bacterianas/farmacología , Transferencia de Energía , Cinética , Ficobilisomas/efectos de los fármacosRESUMEN
In cyanobacteria, phycobilisomes (PBS) act as antennae of the photosynthetic pigment apparatus. They contain brightly colored phycobiliproteins (PBP) and form giant supramolecular complexes (up to 3000-7000 kDa) containing 200 to 500 phycobilin chromophores covalently bound to the proteins. Over ten various PBP are known, which fall into three groups: phycoerythrins, phycocyanins, and allophycocyanins. Hollow disks of PBP trimers and hexamers are arranged into cylinders by colorless linker proteins; the cylinders are then assembled into PBS. Typical semidiscoid PBS consist of a central nucleus formed by three allophycocyanin cylinders and of six lateral cylinders consisting of other PBP and attached as fans to the nucleus. The PBS number, size, and pigment composition in cyanobacteria depend on illumination and other ambient factors. While PBS have certain advantages compared to other antennae, these pigment-protein complexes require more energy than the chlorophyll a/b- and chlorophyll a/c-proteins of oxygenic photosynthetic organisms.
Asunto(s)
Cianobacterias/química , Complejos Multiproteicos/química , Ficobilisomas/química , Ficocianina/química , Ficoeritrina/química , Adaptación Fisiológica , Clorofila/química , Clorofila/clasificación , Clorofila/metabolismo , Cianobacterias/fisiología , Cianobacterias/efectos de la radiación , Evolución Molecular , Luz , Complejos Multiproteicos/metabolismo , Fotosíntesis , Ficobilisomas/metabolismo , Ficobilisomas/efectos de la radiación , Ficobilisomas/ultraestructura , Ficocianina/metabolismo , Ficoeritrina/metabolismo , Multimerización de Proteína , TermodinámicaRESUMEN
Energy transfer pathways between phycobiliproteins chromophores in the phycobilisome (PBS) core of the cyanobacterium Synechocystis sp. PCC 6803 were investigated. The computer 3D model of the PBS core with determination of chromophore to chromophore distance was created. Our kinetic equations based on this model allowed us to describe the relative intensities of the fluorescence emission of the short(peaked at 665 nm) and long-wavelength (peaked at 680 nm) chromophores in the PBS core at low and room temperatures. The difference of emissions of the PBS core at 77 and 293 K are due to the back energy transfer, which is observed at room temperature and is negligible at 77 K.
Asunto(s)
Transferencia de Energía , Ficobilisomas/química , Secuencia de Aminoácidos , Datos de Secuencia Molecular , Ficobilisomas/efectos de la radiación , Synechocystis/química , Rayos UltravioletaRESUMEN
A molecular dynamics study has been undertaken for a model of liquid TIP4P water. Thermal dependencies of water density and radial distribution functions were calculated for model verification. Three methods have been used for calculation of diffusion factor thermal dependencies. Their sensitivity to molecular system size and length of used trajectory has been analyzed. It has been shown that Green-Kubo formula-based approach which associates diffusion factor with speed autocorrelation function integral is preferred in case of short MD simulations. The second approach based on Einstein equation which associates mean square displacement of molecule with time is preferred in case of long simulations. It has been also demonstrated that it is possible to modify the second approach to make it more stable and reliable. This modification is to use a slope of the graph of the mean square displacement on time as the estimation of the diffusion factor instead of the ratio of molecule mean square displacement and time.
Asunto(s)
Modelos Químicos , Agua/químicaRESUMEN
A model of DSPC lipid membrane in gel and liquid-crystalline states has been developed. The parameters have been determined that enable one to calculate the molecular dynamics of lipid bilayers in the full-atromic approximation. The parameters of packing and mobility of lipid molecules for the liquid crystalline state of the bilayer have been calculated. The values agree well with experimental data. Based on the model of the liquid crystalline state of the membrane, a system in the gel-like state has been constructed. The model of the gel-like state reproduces well the packing of lipids in real bilayers, whereas the mobility of molecules in the gel-like state was found to be overestimated.