RESUMEN
Ectodermal mesenchymal stem cells (EMSCs) are cells harvested from the stem cell niche (nasal mucosa) with high therapeutic potential. To the best of our knowledge, however, the antiinflammatory properties of these neural crestderived EMSCs have been rarely reported. The present study aimed to explore the effects of aerosolized EMSCSecretome (EMSCSec) and clarify underlying mechanisms in treating acute lung injury (ALI). EMSCs were isolated by adherent method and identified by immunofluorescence staining. EMSCSec was collected and evaluated using western blotting, BCA and ELISA tests. Then, mouse lung epithelial cells (MLE12) were used to mimic inflammatory stimulation with lipopolysaccharide (LPS). After developing an ALI model through intraperitoneal injection of LPS, mice were treated with an EMSCSec spray. The lung in each group underwent an observation and measurement to preliminarily assess the extent of damage. H&E staining, immunohistochemical staining, immunofluorescence and westernblotting were utilized to further access the impacts of EMSCSec. The results showed that EMSCSec had great antiinflammatory potential and was highly successful in vitro and in vivo. EMSCSec mitigated LPSinduced ALI with low inflammatory cell inflation and mild damage. EMSCSec could regulate inflammation via the NFκB(p50/p65)/NLRP3 pathway. Overall, the present study demonstrated that EMSCSec regulated inflammation, hoping to provide a novel strategy for ALI treatment.
Asunto(s)
Lesión Pulmonar Aguda , Células Madre Mesenquimatosas , Ratones , Animales , Lipopolisacáridos/toxicidad , FN-kappa B/metabolismo , Secretoma , Aerosoles y Gotitas Respiratorias , Lesión Pulmonar Aguda/inducido químicamente , Lesión Pulmonar Aguda/tratamiento farmacológico , Pulmón/metabolismo , Inflamación/tratamiento farmacológico , Antiinflamatorios/uso terapéutico , Células Madre Mesenquimatosas/metabolismoRESUMEN
As a potent clinical strategy, cancer therapy has sparked an academic boom over the past few years. Immune checkpoint inhibitors (ICIs) have been demonstrated to be highly successful. These achievements have progressed cancer treatment and have made an indelible mark on cancer. However, the inherent complexity of cancer means that only part of the population can benefit from this treatment. Pyroptosis is a new suicidal cellular mechanism that induces inflammation by releasing immunogenic cellular components. Inflammatory signaling cascades mediated by pyroptosis commonly inspire numerous cell lysis in immune diseases. Contrariwise, this consequence may be a promising target in cancer research. Therefore, the present study briefly described programmed cell death processes and their potential roles in cancer. Because of the rapid development of bioengineering in cancer, the present study also examined the associated scaffolding available for cancer, highlighting advances in tumor engineering approaches. Ultimately, an improved understanding of pyroptosis and tumor scaffolding might shed light on a combination that can be manipulated for therapeutic purposes.
RESUMEN
Objective: To investigate the association between IL7R rs6897932 and multiple sclerosis (MS) in southern Chinese people. Methods: In total, 147 MS patients and 530 healthy controls were recruited according to the revised McDonald criteria. The TaqMan method was used for genotyping. Results: With genetic models, we can observe that the additive model, the dominant model, and the recessive model of IL7R rs6897932 were significantly associated with MS [additive model: p=0.032; dominant model (adjusted): p<0.001, OR=3.61 (95% CI 2.25-5.83); recessive model (adjusted): p<0.001, OR=6.80 (95% CI 3.49-13.89)]. Conclusion: Our results suggest that IL7R rs6897932 is associated with MS in a southern Chinese population. More and larger MS studies to explore the genetic risk factors of MS are warranted.