RESUMEN
An interventional robot is a means for vascular diagnosis and treatment, and it can perform dredging, releasing drug and operating. Normal hemodynamic indicators are a prerequisite for the application of interventional robots. The current hemodynamic research is limited to the absence of interventional devices or interventional devices in fixed positions. Considering the coupling effect of blood, vessels and robots, based on the bi-directional fluid-structure interaction, using the computational fluid dynamics and particle image velocimetry methods, combined with the sliding and moving mesh technologies, we theoretically and experimentally study the hemodynamic indicators such as blood flow lines, blood pressure, equivalent stress, deformation and wall shear stress of blood vessels when the robot precesses, rotates or does not intervene in the pulsating blood flow. The results show that the intervention of the robot increase the blood flow rate, blood pressure, equivalent stress and deformation of the vessels by 76.4%, 55.4%, 76.5%, and 346%, respectively. The operating mode of the robot during low-speed operation has little impact on the hemodynamic indicators. Using the methyl silicone oil as the experimental fluid, the elastic silicone pipe as the experimental pipe, and the intervention robot having a bioplastic outer shell, the velocity of the fluid around the robot is measured on the developed experimental device for fluid flow field in a pulsating flow when the robot runs. The experimental results are similar to the numerical results. Our work provides an important reference for the hemodynamic study and optimization of the mobile interventional devices.
Asunto(s)
Robótica , Hemodinámica , Presión Sanguínea , Aorta , Flujo Pulsátil , Modelos Cardiovasculares , Velocidad del Flujo Sanguíneo , Simulación por ComputadorRESUMEN
Objectives: Autism spectrum disorder (ASD) is a juvenile onset neurodevelopmental disorder with social impairment and stereotyped behavior as the main symptoms. Unaffected relatives may also exhibit similar ASD features due to genetic factors. Although previous studies have demonstrated atypical brain morphological features as well as task-state brain function abnormalities in unaffected parents with ASD children, it remains unclear the pattern of brain function in the resting state. Methods: A total of 42 unaffected parents of ASD children (pASD) and 39 age-, sex-, and handedness-matched controls were enrolled. Multiple resting-state fMRI (rsfMRI) analyzing methods were applied, including amplitude of low-frequency fluctuation (ALFF), regional homogeneity (ReHo), degree centrality (DC), and functional connectivity (FC), to reveal the functional abnormalities of unaffected parents in ASD-related brain regions. Spearman Rho correlation analysis between imaging metric values and the severity of ASD traits were evaluated as well. Results: ALFF, ReHo, and DC methods all revealed abnormal brain regions in the pASD group, such as the left medial orbitofrontal cortex (mOFC) and rectal gyrus (ROI-1), bilateral supplementary motor area (ROI-2), right caudate nucleus head and right amygdala/para-hippocampal gyrus (ROI-3). FC decreasing was observed between ROI-1 and right anterior cingulate cortex (ACC), ROI-2, and bilateral precuneus. FC enhancing was observed between ROI-3 and right anterior cerebellar lobe, left medial temporal gyrus, left superior temporal gyrus, left medial frontal gyrus, left precentral gyrus, right postcentral gyrus in pASD. In addition, ALFF values in ROI-1, DC values in ROI-3 were positively correlated with AQ scores in pASD (ρ 1 = 0.298, P 1 = 0.007; ρ 2 = 0.220, P 2 = 0.040), while FC values between ROI-1 and right ACC were negatively correlated with AQ scores (ρ3 = -0.334, P 3 = 0.002). Conclusion: rsfMRI metrics could be used as biomarkers to reveal the underlying neurobiological feature of ASD for unaffected parents.
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BACKGROUND: Long non-coding RNA (lncRNA) is reported to be involved in multiple biological processes in numerous human tumors. Furthermore, an increasing number of studies have confirmed the involvement of lncRNA in the initiation and development of human cancers, including cervical cancer (CC). METHODS: The present study explored the potential role of lncRNA glypican 3 antisense transcript 1 (GPC3-AS1) with respect to regulating CC cell proliferation and migration. RESULTS: A quantitative reverse transcriptase-polymerase chain reaction confirmed that GPC3-AS1 was up-regulated in CC cells compared to normal CRL-2614 cells. Loss-of-function assays demonstrated the negative effect of GPC3-AS1 depletion on CC cell proliferation and migration. GPC3-AS1 positively regulated its nearby gene glypican 3 (GPC3). Significant up-regulation of GPC3 was also observed in CC cells, consistently with GPC3-AS1. In addition, GPC3-AS1 and GPC3 synergistically promoted the proliferative and migratory abilities of CC cells. Mechanistic investigation showed that ELK1 acted as the transcription activator of GPC3-AS1 and GPC3, thus contributing to their up-regulation in CC cells. Rescue assays indicated that the ELK1-induced GPC3-AS1/GPC3 axis promoted cell proliferation and migration in CC. CONCLUSIONS: The results of the present study have revealed a novel molecular pathway that can regulate CC cell proliferation and migration, thus providing a new basis for investigating the molecular mechanism associated with CC progression.
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Glipicanos/metabolismo , ARN sin Sentido/metabolismo , ARN Largo no Codificante/metabolismo , Neoplasias del Cuello Uterino/metabolismo , Proteína Elk-1 con Dominio ets/metabolismo , Línea Celular Tumoral , Movimiento Celular/genética , Proliferación Celular/genética , Femenino , Regulación Neoplásica de la Expresión Génica/genética , Glipicanos/genética , Humanos , Motivos de Nucleótidos , Regiones Promotoras Genéticas , ARN sin Sentido/genética , ARN Largo no Codificante/genética , Regulación hacia Arriba , Neoplasias del Cuello Uterino/genética , Proteína Elk-1 con Dominio ets/genéticaRESUMEN
A novel conjugated microporous polymer based on perylene tetraanhydride bisimide (DP2A2) has been synthesized through Sonogashira-Hagihara cross-coupling polymerization of tetrabromo-substituted perylene tetraanhydride bisimide derivative (DPBr2ABr2) with 1,4-diethynylbenzene, whose Brunauer-Emmett-Teller (BET) specific surface area is about 378â¯m2â¯g-1. The fluorescence quenching behaviors of the DP2A2 were investigated. It is found that the DP2A2 shows high sensitivity and selectivity to tracing o-nitrophenol (o-NP) in THF with KsV constant of 2.00â¯×â¯104â¯Lâ¯mol-1. The detection limit (LOD) is 1.50â¯×â¯10-9â¯molâ¯L-1. The possible sensing mechanism for the luminescent quenching of DP2A2 towards o-NP exciting at 365â¯nm was considered the donor-acceptor electron transfer mechanism, which is a combined result from both dynamic (collisional) and static quenching. Moreover, the static quenching process is dominant for DP2A2.
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To examine the amygdala volume in 2-5-year-old preschool children with autism and explore the relationship between amygdala volumes based on MRI findings and clinical features. A total of 39 cases with clinically diagnosed autism were collected. The oblique coronal T1 weighted image (T1WI) sequence was used to measure the volume of amygdala and the MRI signals were measured and analyzed. The data were compared to that of 24 age-matched healthy children and correlated to the clinical manifestations. The autism and the control groups were subject to brain scanning in 1 week after Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition (DSM-IV) review. The 39 cases, diagnosed with autism, were associated with social and behavioral deficits through clinical observation, physical and neurological examination, and assessments according to DSM IV, and the range of ABC scores in the autism group was 47-124, with an average score of 84.7 ± 24.1. Abnormal MRI signals were found in 19/78 (24.4%) amygdala in the autism group, the amygdala lesions showed punctuate or flaky low signal, slightly low signal, low to iso-signal, slightly high signal, or iso to high-signal intensity on T1 weighted three-dimendional fast low angle shot(T1FL3D) images. The right amygdala volume average was 1.088 ± 0.38 cm3, while that of the left amygdala was 1.04 ± 0.41 cm3, without any statistically significant difference (t = 0.533, p = 0.596) in the autism group. Among the 24 cases in the control group, the right amygdala volume average was 0.754 ± 0.194 cm3, while that of the left amygdala was 0.666 ± 0.252 cm3; the autism group had a significantly larger right and left amygdala volumes as compared to the age-matched typically developing group with a significant positive correlation between age and right amygdala volume (r = 0.406, p = 0.01). The preschool children with autism had significantly larger bilateral amygdala volumes as compared to age-matched typically developing children, the amygdala lesions may show abnormal signal. A relationship between age and right amygdala volume in the preschool children with autism was established.